This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 149.86 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 195,896 (6.2%) Reads that were too short: 69,247 (2.2%) Reads written (passing filters): 126,649 (4.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 22,971,663 bp (2.4%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 195896 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 268 773.1 0 268 7 13025 773.1 0 13025 8 99 773.1 0 99 9 84 773.1 0 84 10 104 773.1 0 104 11 85 773.1 0 85 12 76 773.1 0 76 13 160 773.1 0 160 14 104 773.1 0 104 15 76 773.1 0 76 16 69 773.1 0 69 17 76 773.1 0 76 18 88 773.1 0 88 19 92 773.1 0 92 20 85 773.1 0 85 21 75 773.1 0 75 22 58 773.1 0 58 23 97 773.1 0 97 24 79 773.1 0 79 25 116 773.1 0 116 26 102 773.1 0 102 27 99 773.1 0 99 28 101 773.1 0 101 29 63 773.1 0 63 30 64 773.1 0 64 31 70 773.1 0 70 32 83 773.1 0 83 33 86 773.1 0 86 34 91 773.1 0 91 35 66 773.1 0 66 36 75 773.1 0 75 37 76 773.1 0 76 38 84 773.1 0 84 39 85 773.1 0 85 40 70 773.1 0 70 41 70 773.1 0 70 42 69 773.1 0 69 43 112 773.1 0 112 44 102 773.1 0 102 45 97 773.1 0 97 46 113 773.1 0 113 47 76 773.1 0 76 48 62 773.1 0 62 49 78 773.1 0 78 50 87 773.1 0 87 51 97 773.1 0 97 52 88 773.1 0 88 53 59 773.1 0 59 54 74 773.1 0 74 55 63 773.1 0 63 56 91 773.1 0 91 57 92 773.1 0 92 58 97 773.1 0 97 59 69 773.1 0 69 60 84 773.1 0 84 61 86 773.1 0 86 62 82 773.1 0 82 63 74 773.1 0 74 64 80 773.1 0 80 65 79 773.1 0 79 66 109 773.1 0 109 67 103 773.1 0 103 68 113 773.1 0 113 69 105 773.1 0 105 70 107 773.1 0 107 71 88 773.1 0 88 72 106 773.1 0 106 73 151 773.1 0 151 74 238 773.1 0 238 75 160 773.1 0 160 76 283 773.1 0 283 77 180 773.1 0 180 78 355 773.1 0 355 79 83 773.1 0 83 80 85 773.1 0 85 81 82 773.1 0 82 82 89 773.1 0 89 83 84 773.1 0 84 84 95 773.1 0 95 85 96 773.1 0 96 86 79 773.1 0 79 87 66 773.1 0 66 88 72 773.1 0 72 89 67 773.1 0 67 90 72 773.1 0 72 91 108 773.1 0 108 92 81 773.1 0 81 93 99 773.1 0 99 94 84 773.1 0 84 95 88 773.1 0 88 96 89 773.1 0 89 97 109 773.1 0 109 98 85 773.1 0 85 99 105 773.1 0 105 100 87 773.1 0 87 101 99 773.1 0 99 102 105 773.1 0 105 103 130 773.1 0 130 104 109 773.1 0 109 105 114 773.1 0 114 106 110 773.1 0 110 107 279 773.1 0 279 108 240 773.1 0 240 109 292 773.1 0 292 110 151 773.1 0 151 111 123 773.1 0 123 112 93 773.1 0 93 113 150 773.1 0 150 114 130 773.1 0 130 115 139 773.1 0 139 116 131 773.1 0 131 117 123 773.1 0 123 118 130 773.1 0 130 119 181 773.1 0 181 120 256 773.1 0 256 121 298 773.1 0 298 122 135 773.1 0 135 123 226 773.1 0 226 124 342 773.1 0 342 125 200 773.1 0 200 126 172 773.1 0 172 127 282 773.1 0 282 128 273 773.1 0 273 129 324 773.1 0 324 130 320 773.1 0 320 131 478 773.1 0 478 132 819 773.1 0 819 133 4602 773.1 0 4602 134 28934 773.1 0 28934 135 16697 773.1 0 16697 136 24844 773.1 0 24844 137 430 773.1 0 430 138 174 773.1 0 174 139 170 773.1 0 170 140 189 773.1 0 189 141 265 773.1 0 265 142 187 773.1 0 187 143 130 773.1 0 130 144 129 773.1 0 129 145 136 773.1 0 136 146 123 773.1 0 123 147 446 773.1 0 446 148 198 773.1 0 198 149 286 773.1 0 286 150 121 773.1 0 121 151 142 773.1 0 142 152 198 773.1 0 198 153 254 773.1 0 254 154 1409 773.1 0 1409 155 1366 773.1 0 1366 156 1125 773.1 0 1125 157 4133 773.1 0 4133 158 2223 773.1 0 2223 159 2729 773.1 0 2729 160 251 773.1 0 251 161 269 773.1 0 269 162 212 773.1 0 212 163 140 773.1 0 140 164 232 773.1 0 232 165 215 773.1 0 215 166 239 773.1 0 239 167 219 773.1 0 219 168 110 773.1 0 110 169 94 773.1 0 94 170 86 773.1 0 86 171 77 773.1 0 77 172 103 773.1 0 103 173 142 773.1 0 142 174 144 773.1 0 144 175 133 773.1 0 133 176 100 773.1 0 100 177 97 773.1 0 97 178 95 773.1 0 95 179 88 773.1 0 88 180 97 773.1 0 97 181 138 773.1 0 138 182 154 773.1 0 154 183 100 773.1 0 100 184 157 773.1 0 157 185 111 773.1 0 111 186 178 773.1 0 178 187 96 773.1 0 96 188 117 773.1 0 117 189 143 773.1 0 143 190 254 773.1 0 254 191 216 773.1 0 216 192 176 773.1 0 176 193 117 773.1 0 117 194 103 773.1 0 103 195 53 773.1 0 53 196 110 773.1 0 110 197 80 773.1 0 80 198 62 773.1 0 62 199 92 773.1 0 92 200 129 773.1 0 129 201 111 773.1 0 111 202 139 773.1 0 139 203 118 773.1 0 118 204 112 773.1 0 112 205 98 773.1 0 98 206 115 773.1 0 115 207 141 773.1 0 141 208 120 773.1 0 120 209 112 773.1 0 112 210 98 773.1 0 98 211 78 773.1 0 78 212 104 773.1 0 104 213 139 773.1 0 139 214 167 773.1 0 167 215 185 773.1 0 185 216 138 773.1 0 138 217 111 773.1 0 111 218 104 773.1 0 104 219 117 773.1 0 117 220 145 773.1 0 145 221 149 773.1 0 149 222 124 773.1 0 124 223 108 773.1 0 108 224 112 773.1 0 112 225 164 773.1 0 164 226 120 773.1 0 120 227 136 773.1 0 136 228 153 773.1 0 153 229 132 773.1 0 132 230 120 773.1 0 120 231 109 773.1 0 109 232 194 773.1 0 194 233 190 773.1 0 190 234 176 773.1 0 176 235 137 773.1 0 137 236 84 773.1 0 84 237 104 773.1 0 104 238 117 773.1 0 117 239 109 773.1 0 109 240 143 773.1 0 143 241 123 773.1 0 123 242 128 773.1 0 128 243 123 773.1 0 123 244 104 773.1 0 104 245 115 773.1 0 115 246 99 773.1 0 99 247 108 773.1 0 108 248 100 773.1 0 100 249 70 773.1 0 70 250 130 773.1 0 130 251 211 773.1 0 211 252 385 773.1 0 385 253 282 773.1 0 282 254 331 773.1 0 331 255 126 773.1 0 126 256 111 773.1 0 111 257 121 773.1 0 121 258 121 773.1 0 121 259 120 773.1 0 120 260 109 773.1 0 109 261 140 773.1 0 140 262 172 773.1 0 172 263 162 773.1 0 162 264 135 773.1 0 135 265 102 773.1 0 102 266 119 773.1 0 119 267 147 773.1 0 147 268 328 773.1 0 328 269 319 773.1 0 319 270 953 773.1 0 953 271 1044 773.1 0 1044 272 544 773.1 0 544 273 178 773.1 0 178 274 227 773.1 0 227 275 451 773.1 0 451 276 1777 773.1 0 1777 277 2147 773.1 0 2147 278 2826 773.1 0 2826 279 988 773.1 0 988 280 253 773.1 0 253 281 240 773.1 0 240 282 311 773.1 0 311 283 215 773.1 0 215 284 232 773.1 0 232 285 361 773.1 0 361 286 192 773.1 0 192 287 150 773.1 0 150 288 305 773.1 0 305 289 500 773.1 0 500 290 840 773.1 0 840 291 587 773.1 0 587 292 360 773.1 0 360 293 474 773.1 0 474 294 715 773.1 0 715 295 13304 773.1 0 13304 296 11820 773.1 0 11820 297 17055 773.1 0 17055 298 151 773.1 0 151 299 121 773.1 0 121 300 132 773.1 0 132 301 131 773.1 0 131 Finished in 7.78 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 126,649 Reads with adapters: 6,034 (4.8%) Reads that were too short: 47 (0.0%) Reads written (passing filters): 5,987 (4.7%) Total basepairs processed: 22,971,663 bp Total written (filtered): 1,637,256 bp (7.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6034 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 6 0.0 3 0 0 5 1 19 54 0.0 3 0 45 4 5 20 5868 0.0 4 5172 605 66 17 8 21 10 0.0 4 2 8 22 10 0.0 4 6 1 0 0 3 23 11 0.0 4 0 0 0 5 6 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 81 12 0.0 4 0 0 0 1 11 82 6 0.0 4 0 0 0 0 6 83 2 0.0 4 0 0 0 0 2 86 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 102 11 0.0 4 0 0 0 0 11 111 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 127 19 0.0 4 0 0 0 0 19 150 2 0.0 4 0 0 1 0 1 159 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 5,987 Reads with adapters: 287 (4.8%) Reads written (passing filters): 5,987 (100.0%) Total basepairs processed: 1,637,256 bp Total written (filtered): 1,634,012 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 287 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.0% C: 8.0% G: 84.7% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 19 93.5 0 19 4 2 23.4 0 2 5 1 5.8 0 1 6 1 1.5 0 1 10 6 0.0 1 1 5 11 217 0.0 1 4 213 12 36 0.0 1 0 36 13 1 0.0 1 0 1 21 2 0.0 1 2 24 1 0.0 1 1 210 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.