This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 149.88 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 217,013 (6.9%) Reads that were too short: 88,134 (2.8%) Reads written (passing filters): 128,879 (4.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 23,559,763 bp (2.5%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 217013 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 241 773.1 0 241 7 13204 773.1 0 13204 8 132 773.1 0 132 9 120 773.1 0 120 10 157 773.1 0 157 11 118 773.1 0 118 12 106 773.1 0 106 13 152 773.1 0 152 14 130 773.1 0 130 15 106 773.1 0 106 16 129 773.1 0 129 17 92 773.1 0 92 18 124 773.1 0 124 19 115 773.1 0 115 20 124 773.1 0 124 21 124 773.1 0 124 22 102 773.1 0 102 23 139 773.1 0 139 24 114 773.1 0 114 25 118 773.1 0 118 26 121 773.1 0 121 27 126 773.1 0 126 28 92 773.1 0 92 29 95 773.1 0 95 30 111 773.1 0 111 31 101 773.1 0 101 32 115 773.1 0 115 33 134 773.1 0 134 34 131 773.1 0 131 35 137 773.1 0 137 36 105 773.1 0 105 37 108 773.1 0 108 38 91 773.1 0 91 39 113 773.1 0 113 40 96 773.1 0 96 41 90 773.1 0 90 42 109 773.1 0 109 43 111 773.1 0 111 44 93 773.1 0 93 45 96 773.1 0 96 46 104 773.1 0 104 47 110 773.1 0 110 48 113 773.1 0 113 49 93 773.1 0 93 50 129 773.1 0 129 51 105 773.1 0 105 52 100 773.1 0 100 53 116 773.1 0 116 54 100 773.1 0 100 55 101 773.1 0 101 56 123 773.1 0 123 57 127 773.1 0 127 58 117 773.1 0 117 59 96 773.1 0 96 60 121 773.1 0 121 61 123 773.1 0 123 62 110 773.1 0 110 63 123 773.1 0 123 64 136 773.1 0 136 65 101 773.1 0 101 66 107 773.1 0 107 67 133 773.1 0 133 68 131 773.1 0 131 69 191 773.1 0 191 70 134 773.1 0 134 71 106 773.1 0 106 72 158 773.1 0 158 73 134 773.1 0 134 74 244 773.1 0 244 75 176 773.1 0 176 76 246 773.1 0 246 77 228 773.1 0 228 78 306 773.1 0 306 79 126 773.1 0 126 80 147 773.1 0 147 81 139 773.1 0 139 82 139 773.1 0 139 83 126 773.1 0 126 84 163 773.1 0 163 85 151 773.1 0 151 86 108 773.1 0 108 87 138 773.1 0 138 88 131 773.1 0 131 89 151 773.1 0 151 90 138 773.1 0 138 91 151 773.1 0 151 92 118 773.1 0 118 93 137 773.1 0 137 94 120 773.1 0 120 95 144 773.1 0 144 96 150 773.1 0 150 97 137 773.1 0 137 98 134 773.1 0 134 99 132 773.1 0 132 100 117 773.1 0 117 101 135 773.1 0 135 102 114 773.1 0 114 103 172 773.1 0 172 104 140 773.1 0 140 105 147 773.1 0 147 106 159 773.1 0 159 107 272 773.1 0 272 108 225 773.1 0 225 109 299 773.1 0 299 110 190 773.1 0 190 111 168 773.1 0 168 112 130 773.1 0 130 113 181 773.1 0 181 114 178 773.1 0 178 115 154 773.1 0 154 116 141 773.1 0 141 117 180 773.1 0 180 118 193 773.1 0 193 119 183 773.1 0 183 120 300 773.1 0 300 121 341 773.1 0 341 122 216 773.1 0 216 123 244 773.1 0 244 124 306 773.1 0 306 125 218 773.1 0 218 126 233 773.1 0 233 127 275 773.1 0 275 128 260 773.1 0 260 129 348 773.1 0 348 130 319 773.1 0 319 131 488 773.1 0 488 132 669 773.1 0 669 133 4411 773.1 0 4411 134 27339 773.1 0 27339 135 15821 773.1 0 15821 136 24433 773.1 0 24433 137 370 773.1 0 370 138 215 773.1 0 215 139 210 773.1 0 210 140 175 773.1 0 175 141 220 773.1 0 220 142 194 773.1 0 194 143 149 773.1 0 149 144 138 773.1 0 138 145 155 773.1 0 155 146 168 773.1 0 168 147 309 773.1 0 309 148 201 773.1 0 201 149 250 773.1 0 250 150 168 773.1 0 168 151 134 773.1 0 134 152 203 773.1 0 203 153 262 773.1 0 262 154 1417 773.1 0 1417 155 1383 773.1 0 1383 156 1165 773.1 0 1165 157 4714 773.1 0 4714 158 2668 773.1 0 2668 159 3073 773.1 0 3073 160 201 773.1 0 201 161 175 773.1 0 175 162 175 773.1 0 175 163 125 773.1 0 125 164 154 773.1 0 154 165 180 773.1 0 180 166 171 773.1 0 171 167 203 773.1 0 203 168 124 773.1 0 124 169 152 773.1 0 152 170 103 773.1 0 103 171 123 773.1 0 123 172 122 773.1 0 122 173 136 773.1 0 136 174 172 773.1 0 172 175 140 773.1 0 140 176 145 773.1 0 145 177 116 773.1 0 116 178 133 773.1 0 133 179 97 773.1 0 97 180 101 773.1 0 101 181 152 773.1 0 152 182 151 773.1 0 151 183 118 773.1 0 118 184 123 773.1 0 123 185 116 773.1 0 116 186 160 773.1 0 160 187 131 773.1 0 131 188 124 773.1 0 124 189 115 773.1 0 115 190 280 773.1 0 280 191 210 773.1 0 210 192 209 773.1 0 209 193 137 773.1 0 137 194 111 773.1 0 111 195 97 773.1 0 97 196 136 773.1 0 136 197 94 773.1 0 94 198 111 773.1 0 111 199 123 773.1 0 123 200 125 773.1 0 125 201 124 773.1 0 124 202 142 773.1 0 142 203 128 773.1 0 128 204 117 773.1 0 117 205 131 773.1 0 131 206 142 773.1 0 142 207 116 773.1 0 116 208 116 773.1 0 116 209 90 773.1 0 90 210 94 773.1 0 94 211 107 773.1 0 107 212 111 773.1 0 111 213 147 773.1 0 147 214 162 773.1 0 162 215 144 773.1 0 144 216 133 773.1 0 133 217 127 773.1 0 127 218 123 773.1 0 123 219 114 773.1 0 114 220 128 773.1 0 128 221 152 773.1 0 152 222 123 773.1 0 123 223 94 773.1 0 94 224 131 773.1 0 131 225 200 773.1 0 200 226 155 773.1 0 155 227 120 773.1 0 120 228 138 773.1 0 138 229 107 773.1 0 107 230 111 773.1 0 111 231 128 773.1 0 128 232 130 773.1 0 130 233 135 773.1 0 135 234 126 773.1 0 126 235 129 773.1 0 129 236 98 773.1 0 98 237 111 773.1 0 111 238 130 773.1 0 130 239 116 773.1 0 116 240 132 773.1 0 132 241 128 773.1 0 128 242 106 773.1 0 106 243 114 773.1 0 114 244 127 773.1 0 127 245 114 773.1 0 114 246 118 773.1 0 118 247 123 773.1 0 123 248 99 773.1 0 99 249 102 773.1 0 102 250 120 773.1 0 120 251 192 773.1 0 192 252 310 773.1 0 310 253 271 773.1 0 271 254 250 773.1 0 250 255 91 773.1 0 91 256 97 773.1 0 97 257 128 773.1 0 128 258 119 773.1 0 119 259 126 773.1 0 126 260 124 773.1 0 124 261 113 773.1 0 113 262 144 773.1 0 144 263 102 773.1 0 102 264 136 773.1 0 136 265 111 773.1 0 111 266 123 773.1 0 123 267 119 773.1 0 119 268 229 773.1 0 229 269 256 773.1 0 256 270 951 773.1 0 951 271 1080 773.1 0 1080 272 587 773.1 0 587 273 168 773.1 0 168 274 234 773.1 0 234 275 418 773.1 0 418 276 2215 773.1 0 2215 277 2464 773.1 0 2464 278 3413 773.1 0 3413 279 1018 773.1 0 1018 280 214 773.1 0 214 281 256 773.1 0 256 282 366 773.1 0 366 283 197 773.1 0 197 284 237 773.1 0 237 285 466 773.1 0 466 286 201 773.1 0 201 287 185 773.1 0 185 288 396 773.1 0 396 289 629 773.1 0 629 290 921 773.1 0 921 291 637 773.1 0 637 292 486 773.1 0 486 293 533 773.1 0 533 294 786 773.1 0 786 295 18329 773.1 0 18329 296 16723 773.1 0 16723 297 24419 773.1 0 24419 298 178 773.1 0 178 299 91 773.1 0 91 300 106 773.1 0 106 301 100 773.1 0 100 Finished in 7.25 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 128,879 Reads with adapters: 6,980 (5.4%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 6,931 (5.4%) Total basepairs processed: 23,559,763 bp Total written (filtered): 1,758,961 bp (7.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6980 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 17 0.0 3 0 15 2 17 5923 0.0 3 5010 850 62 1 18 7 0.0 3 0 5 2 19 2 0.0 3 1 0 0 1 20 11 0.0 3 0 0 0 11 27 2 0.0 3 0 0 0 2 32 83 0.0 3 0 0 1 82 35 10 0.0 3 0 0 0 10 47 1 0.0 3 0 0 1 50 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 859 0.0 3 0 0 0 859 59 4 0.0 3 0 0 0 4 61 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 83 5 0.0 3 0 1 0 4 85 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 23 0.0 3 0 0 0 23 95 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 1 151 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 6,931 Reads with adapters: 20 (0.3%) Reads written (passing filters): 6,931 (100.0%) Total basepairs processed: 1,758,961 bp Total written (filtered): 1,758,898 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 20 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.0% C: 10.0% G: 40.0% T: 40.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 17 108.3 0 17 4 3 27.1 0 3