This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 154.92 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 400,699 (12.7%) Reads that were too short: 13,580 (0.4%) Reads written (passing filters): 387,119 (12.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 109,907,310 bp (11.5%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 400699 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3213 773.1 0 3213 7 5584 773.1 0 5584 8 203410 773.1 0 203410 9 145392 773.1 0 145392 10 140 773.1 0 140 11 9 773.1 0 9 12 4 773.1 0 4 13 30 773.1 0 30 14 107 773.1 0 107 15 126 773.1 0 126 16 32 773.1 0 32 17 9 773.1 0 9 18 49 773.1 0 49 19 38 773.1 0 38 20 30 773.1 0 30 21 4 773.1 0 4 22 1 773.1 0 1 23 4 773.1 0 4 24 2 773.1 0 2 25 4 773.1 0 4 26 8 773.1 0 8 27 17 773.1 0 17 28 10 773.1 0 10 29 5 773.1 0 5 30 3 773.1 0 3 31 2 773.1 0 2 32 5 773.1 0 5 33 6 773.1 0 6 34 4 773.1 0 4 35 10 773.1 0 10 36 4 773.1 0 4 38 4 773.1 0 4 39 6 773.1 0 6 40 12 773.1 0 12 41 16 773.1 0 16 42 6 773.1 0 6 43 92 773.1 0 92 44 272 773.1 0 272 45 341 773.1 0 341 46 93 773.1 0 93 47 11 773.1 0 11 48 7 773.1 0 7 49 5 773.1 0 5 50 33 773.1 0 33 51 7 773.1 0 7 52 18 773.1 0 18 53 14 773.1 0 14 54 11 773.1 0 11 55 4 773.1 0 4 56 9 773.1 0 9 57 11 773.1 0 11 58 3 773.1 0 3 59 48 773.1 0 48 60 42 773.1 0 42 61 20 773.1 0 20 62 20 773.1 0 20 63 16 773.1 0 16 64 34 773.1 0 34 65 132 773.1 0 132 66 42 773.1 0 42 67 64 773.1 0 64 68 48 773.1 0 48 69 40 773.1 0 40 70 23 773.1 0 23 71 23 773.1 0 23 72 45 773.1 0 45 73 73 773.1 0 73 74 79 773.1 0 79 75 110 773.1 0 110 76 352 773.1 0 352 77 4058 773.1 0 4058 78 2524 773.1 0 2524 79 5887 773.1 0 5887 80 45 773.1 0 45 81 11 773.1 0 11 82 42 773.1 0 42 83 15 773.1 0 15 84 23 773.1 0 23 85 12 773.1 0 12 86 34 773.1 0 34 87 2 773.1 0 2 88 5 773.1 0 5 89 4 773.1 0 4 90 10 773.1 0 10 91 13 773.1 0 13 92 40 773.1 0 40 93 75 773.1 0 75 94 23 773.1 0 23 95 11 773.1 0 11 96 26 773.1 0 26 97 7 773.1 0 7 98 20 773.1 0 20 99 12 773.1 0 12 100 5 773.1 0 5 101 11 773.1 0 11 102 4 773.1 0 4 103 10 773.1 0 10 104 4 773.1 0 4 105 5 773.1 0 5 106 3 773.1 0 3 107 6 773.1 0 6 108 4 773.1 0 4 109 3 773.1 0 3 110 10 773.1 0 10 111 4 773.1 0 4 112 10 773.1 0 10 113 17 773.1 0 17 114 41 773.1 0 41 115 52 773.1 0 52 116 102 773.1 0 102 117 7 773.1 0 7 118 5 773.1 0 5 119 5 773.1 0 5 120 13 773.1 0 13 121 19 773.1 0 19 122 22 773.1 0 22 123 25 773.1 0 25 124 12 773.1 0 12 125 16 773.1 0 16 126 18 773.1 0 18 127 29 773.1 0 29 128 26 773.1 0 26 129 18 773.1 0 18 130 64 773.1 0 64 131 13 773.1 0 13 132 18 773.1 0 18 133 15 773.1 0 15 134 34 773.1 0 34 135 92 773.1 0 92 136 152 773.1 0 152 137 296 773.1 0 296 138 209 773.1 0 209 139 173 773.1 0 173 140 54 773.1 0 54 141 41 773.1 0 41 142 48 773.1 0 48 143 33 773.1 0 33 144 18 773.1 0 18 145 11 773.1 0 11 146 14 773.1 0 14 147 13 773.1 0 13 148 19 773.1 0 19 149 28 773.1 0 28 150 29 773.1 0 29 151 14 773.1 0 14 152 9 773.1 0 9 153 16 773.1 0 16 154 21 773.1 0 21 155 24 773.1 0 24 156 9 773.1 0 9 157 22 773.1 0 22 158 55 773.1 0 55 159 10 773.1 0 10 160 9 773.1 0 9 161 17 773.1 0 17 162 21 773.1 0 21 163 42 773.1 0 42 164 59 773.1 0 59 165 79 773.1 0 79 166 35 773.1 0 35 167 8 773.1 0 8 168 19 773.1 0 19 169 19 773.1 0 19 170 30 773.1 0 30 171 42 773.1 0 42 172 54 773.1 0 54 173 560 773.1 0 560 174 410 773.1 0 410 175 613 773.1 0 613 176 80 773.1 0 80 177 27 773.1 0 27 178 31 773.1 0 31 179 90 773.1 0 90 180 544 773.1 0 544 181 308 773.1 0 308 182 306 773.1 0 306 183 26 773.1 0 26 184 29 773.1 0 29 185 57 773.1 0 57 186 38 773.1 0 38 187 66 773.1 0 66 188 65 773.1 0 65 189 105 773.1 0 105 190 411 773.1 0 411 191 2531 773.1 0 2531 192 2721 773.1 0 2721 193 1800 773.1 0 1800 194 308 773.1 0 308 195 13 773.1 0 13 196 7 773.1 0 7 197 23 773.1 0 23 198 32 773.1 0 32 199 35 773.1 0 35 200 20 773.1 0 20 201 22 773.1 0 22 202 173 773.1 0 173 203 492 773.1 0 492 204 452 773.1 0 452 205 251 773.1 0 251 206 18 773.1 0 18 207 21 773.1 0 21 208 149 773.1 0 149 209 147 773.1 0 147 210 190 773.1 0 190 211 53 773.1 0 53 212 13 773.1 0 13 213 12 773.1 0 12 214 17 773.1 0 17 215 57 773.1 0 57 216 49 773.1 0 49 217 316 773.1 0 316 218 43 773.1 0 43 219 24 773.1 0 24 220 30 773.1 0 30 221 6 773.1 0 6 222 9 773.1 0 9 223 14 773.1 0 14 224 17 773.1 0 17 225 22 773.1 0 22 226 30 773.1 0 30 227 40 773.1 0 40 228 23 773.1 0 23 229 44 773.1 0 44 230 12 773.1 0 12 231 11 773.1 0 11 232 38 773.1 0 38 233 32 773.1 0 32 234 61 773.1 0 61 235 38 773.1 0 38 236 45 773.1 0 45 237 22 773.1 0 22 238 9 773.1 0 9 239 21 773.1 0 21 240 44 773.1 0 44 241 14 773.1 0 14 242 61 773.1 0 61 243 26 773.1 0 26 244 30 773.1 0 30 245 31 773.1 0 31 246 14 773.1 0 14 247 47 773.1 0 47 248 47 773.1 0 47 249 13 773.1 0 13 250 28 773.1 0 28 251 75 773.1 0 75 252 251 773.1 0 251 253 300 773.1 0 300 254 239 773.1 0 239 255 98 773.1 0 98 256 13 773.1 0 13 257 35 773.1 0 35 258 303 773.1 0 303 259 370 773.1 0 370 260 423 773.1 0 423 261 620 773.1 0 620 262 501 773.1 0 501 263 407 773.1 0 407 264 258 773.1 0 258 265 293 773.1 0 293 266 111 773.1 0 111 267 45 773.1 0 45 268 22 773.1 0 22 269 17 773.1 0 17 270 36 773.1 0 36 271 54 773.1 0 54 272 96 773.1 0 96 273 85 773.1 0 85 274 59 773.1 0 59 275 56 773.1 0 56 276 41 773.1 0 41 277 58 773.1 0 58 278 71 773.1 0 71 279 101 773.1 0 101 280 89 773.1 0 89 281 52 773.1 0 52 282 42 773.1 0 42 283 190 773.1 0 190 284 1404 773.1 0 1404 285 964 773.1 0 964 286 1618 773.1 0 1618 287 78 773.1 0 78 288 25 773.1 0 25 289 33 773.1 0 33 290 39 773.1 0 39 291 46 773.1 0 46 292 35 773.1 0 35 293 22 773.1 0 22 294 41 773.1 0 41 295 73 773.1 0 73 296 105 773.1 0 105 297 83 773.1 0 83 298 98 773.1 0 98 299 28 773.1 0 28 300 31 773.1 0 31 301 90 773.1 0 90 Finished in 30.04 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 387,119 Reads with adapters: 158,457 (40.9%) Reads that were too short: 118 (0.0%) Reads written (passing filters): 158,339 (40.9%) Total basepairs processed: 109,907,310 bp Total written (filtered): 43,055,312 bp (39.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 158457 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 20 0.0 3 0 0 0 13 7 18 91 0.0 3 0 0 53 23 15 19 2547 0.0 3 0 2279 124 98 46 20 154331 0.0 4 146162 6539 1207 320 103 21 447 0.0 4 108 303 30 3 3 22 48 0.0 4 5 2 24 5 12 23 591 0.0 4 1 2 1 410 177 24 4 0.0 4 1 0 0 0 3 25 1 0.0 4 0 0 1 26 1 0.0 4 1 27 4 0.0 4 0 0 0 0 4 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 47 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 4 0.0 4 0 0 0 0 4 93 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 1 103 1 0.0 4 0 0 0 0 1 112 1 0.0 4 1 113 1 0.0 4 1 122 1 0.0 4 1 126 1 0.0 4 0 0 0 0 1 127 178 0.0 4 0 0 0 0 178 128 5 0.0 4 0 0 0 0 5 129 4 0.0 4 1 0 0 0 3 130 3 0.0 4 0 0 0 0 3 133 1 0.0 4 0 0 0 0 1 138 2 0.0 4 1 0 0 0 1 140 1 0.0 4 1 142 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 1 146 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 13 0.0 4 0 0 0 1 12 154 1 0.0 4 1 158 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 172 15 0.0 4 0 0 0 0 15 176 1 0.0 4 1 186 1 0.0 4 1 189 2 0.0 4 0 0 0 1 1 191 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 197 2 0.0 4 0 1 1 205 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 209 3 0.0 4 0 0 0 0 3 211 1 0.0 4 0 0 0 0 1 212 1 0.0 4 1 213 2 0.0 4 0 0 0 0 2 214 2 0.0 4 1 0 0 0 1 216 1 0.0 4 0 0 0 0 1 217 2 0.0 4 0 0 0 0 2 218 3 0.0 4 3 220 2 0.0 4 0 0 0 1 1 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 3 0.0 4 0 0 0 0 3 228 12 0.0 4 0 0 0 1 11 229 13 0.0 4 0 0 0 2 11 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 233 3 0.0 4 0 0 0 0 3 234 4 0.0 4 0 0 0 0 4 235 3 0.0 4 0 0 0 0 3 236 2 0.0 4 0 0 0 0 2 237 4 0.0 4 0 0 0 0 4 239 2 0.0 4 1 0 0 1 241 1 0.0 4 0 0 0 1 249 2 0.0 4 0 0 0 0 2 251 2 0.0 4 0 0 1 0 1 252 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 1 262 3 0.0 4 0 0 0 0 3 263 4 0.0 4 0 0 0 0 4 264 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 6 0.0 4 0 0 0 0 6 283 2 0.0 4 0 0 0 0 2 285 2 0.0 4 0 0 0 0 2 292 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.67 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 158,339 Reads with adapters: 40,552 (25.6%) Reads written (passing filters): 158,339 (100.0%) Total basepairs processed: 43,055,312 bp Total written (filtered): 42,599,515 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 40552 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 4.9% G: 91.3% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 81 2474.0 0 81 4 33 618.5 0 33 5 2 154.6 0 2 6 8 38.7 0 8 7 7 9.7 0 7 8 8 2.4 0 8 9 17378 0.6 0 949 16429 10 16958 0.2 1 1033 15925 11 5591 0.0 1 144 5447 12 82 0.0 1 2 80 13 43 0.0 1 1 42 14 4 0.0 1 0 4 15 1 0.0 1 0 1 17 5 0.0 1 2 3 18 1 0.0 1 1 19 2 0.0 1 2 20 1 0.0 1 1 22 8 0.0 1 4 4 34 1 0.0 1 0 1 43 1 0.0 1 0 1 54 1 0.0 1 1 71 1 0.0 1 1 80 1 0.0 1 0 1 92 1 0.0 1 1 114 27 0.0 1 27 120 1 0.0 1 1 134 1 0.0 1 1 147 2 0.0 1 2 150 18 0.0 1 17 1 152 1 0.0 1 0 1 153 1 0.0 1 0 1 158 1 0.0 1 0 1 169 1 0.0 1 0 1 189 4 0.0 1 0 4 206 1 0.0 1 1 207 3 0.0 1 3 208 217 0.0 1 187 30 209 29 0.0 1 23 6 210 10 0.0 1 7 3 211 2 0.0 1 2 212 1 0.0 1 1 219 1 0.0 1 1 223 1 0.0 1 1 224 1 0.0 1 1 226 5 0.0 1 5 228 1 0.0 1 0 1 229 2 0.0 1 1 1 242 1 0.0 1 1 247 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.