This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 155.36 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 404,954 (12.8%) Reads that were too short: 6,957 (0.2%) Reads written (passing filters): 397,997 (12.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 113,399,937 bp (11.9%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 404954 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2951 773.1 0 2951 7 4478 773.1 0 4478 8 184516 773.1 0 184516 9 178994 773.1 0 178994 10 143 773.1 0 143 11 11 773.1 0 11 12 4 773.1 0 4 13 7 773.1 0 7 14 5 773.1 0 5 15 5 773.1 0 5 16 3 773.1 0 3 17 2 773.1 0 2 18 10 773.1 0 10 19 16 773.1 0 16 20 11 773.1 0 11 21 6 773.1 0 6 22 3 773.1 0 3 24 2 773.1 0 2 25 1 773.1 0 1 26 2 773.1 0 2 27 2 773.1 0 2 28 1 773.1 0 1 29 4 773.1 0 4 30 2 773.1 0 2 31 3 773.1 0 3 33 6 773.1 0 6 34 1 773.1 0 1 35 18 773.1 0 18 36 2 773.1 0 2 37 3 773.1 0 3 38 1 773.1 0 1 39 3 773.1 0 3 40 16 773.1 0 16 41 7 773.1 0 7 42 8 773.1 0 8 43 8 773.1 0 8 44 45 773.1 0 45 45 65 773.1 0 65 46 33 773.1 0 33 47 7 773.1 0 7 48 4 773.1 0 4 49 5 773.1 0 5 50 24 773.1 0 24 51 9 773.1 0 9 52 8 773.1 0 8 53 5 773.1 0 5 54 6 773.1 0 6 55 3 773.1 0 3 56 7 773.1 0 7 57 15 773.1 0 15 58 8 773.1 0 8 59 18 773.1 0 18 60 19 773.1 0 19 61 7 773.1 0 7 62 17 773.1 0 17 63 21 773.1 0 21 64 44 773.1 0 44 65 135 773.1 0 135 66 22 773.1 0 22 67 31 773.1 0 31 68 19 773.1 0 19 69 12 773.1 0 12 70 14 773.1 0 14 71 27 773.1 0 27 72 47 773.1 0 47 73 73 773.1 0 73 74 74 773.1 0 74 75 154 773.1 0 154 76 320 773.1 0 320 77 4604 773.1 0 4604 78 2777 773.1 0 2777 79 6460 773.1 0 6460 80 19 773.1 0 19 81 6 773.1 0 6 82 44 773.1 0 44 83 8 773.1 0 8 84 8 773.1 0 8 85 5 773.1 0 5 86 8 773.1 0 8 87 5 773.1 0 5 88 5 773.1 0 5 89 2 773.1 0 2 90 13 773.1 0 13 91 16 773.1 0 16 92 22 773.1 0 22 93 34 773.1 0 34 94 10 773.1 0 10 95 12 773.1 0 12 96 30 773.1 0 30 97 9 773.1 0 9 98 24 773.1 0 24 99 8 773.1 0 8 100 10 773.1 0 10 101 9 773.1 0 9 102 5 773.1 0 5 103 1 773.1 0 1 104 3 773.1 0 3 105 9 773.1 0 9 106 3 773.1 0 3 107 8 773.1 0 8 108 7 773.1 0 7 109 4 773.1 0 4 110 10 773.1 0 10 111 6 773.1 0 6 112 8 773.1 0 8 113 11 773.1 0 11 114 15 773.1 0 15 115 15 773.1 0 15 116 78 773.1 0 78 117 9 773.1 0 9 118 1 773.1 0 1 119 7 773.1 0 7 120 6 773.1 0 6 121 12 773.1 0 12 122 10 773.1 0 10 123 13 773.1 0 13 124 6 773.1 0 6 125 8 773.1 0 8 126 7 773.1 0 7 127 4 773.1 0 4 128 6 773.1 0 6 129 7 773.1 0 7 130 41 773.1 0 41 131 9 773.1 0 9 132 8 773.1 0 8 133 13 773.1 0 13 134 16 773.1 0 16 135 32 773.1 0 32 136 67 773.1 0 67 137 255 773.1 0 255 138 197 773.1 0 197 139 158 773.1 0 158 140 44 773.1 0 44 141 24 773.1 0 24 142 51 773.1 0 51 143 11 773.1 0 11 144 7 773.1 0 7 145 6 773.1 0 6 146 3 773.1 0 3 147 7 773.1 0 7 148 10 773.1 0 10 149 12 773.1 0 12 150 14 773.1 0 14 151 8 773.1 0 8 152 12 773.1 0 12 153 6 773.1 0 6 154 12 773.1 0 12 155 9 773.1 0 9 156 13 773.1 0 13 157 7 773.1 0 7 158 43 773.1 0 43 159 4 773.1 0 4 160 14 773.1 0 14 161 11 773.1 0 11 162 12 773.1 0 12 163 24 773.1 0 24 164 30 773.1 0 30 165 25 773.1 0 25 166 23 773.1 0 23 167 9 773.1 0 9 168 10 773.1 0 10 169 7 773.1 0 7 170 7 773.1 0 7 171 20 773.1 0 20 172 14 773.1 0 14 173 280 773.1 0 280 174 240 773.1 0 240 175 271 773.1 0 271 176 29 773.1 0 29 177 12 773.1 0 12 178 25 773.1 0 25 179 72 773.1 0 72 180 383 773.1 0 383 181 193 773.1 0 193 182 151 773.1 0 151 183 10 773.1 0 10 184 24 773.1 0 24 185 18 773.1 0 18 186 21 773.1 0 21 187 40 773.1 0 40 188 27 773.1 0 27 189 93 773.1 0 93 190 336 773.1 0 336 191 2701 773.1 0 2701 192 2786 773.1 0 2786 193 1722 773.1 0 1722 194 201 773.1 0 201 195 14 773.1 0 14 196 2 773.1 0 2 197 8 773.1 0 8 198 9 773.1 0 9 199 16 773.1 0 16 200 10 773.1 0 10 201 18 773.1 0 18 202 162 773.1 0 162 203 282 773.1 0 282 204 327 773.1 0 327 205 158 773.1 0 158 206 12 773.1 0 12 207 9 773.1 0 9 208 36 773.1 0 36 209 38 773.1 0 38 210 28 773.1 0 28 211 14 773.1 0 14 212 4 773.1 0 4 213 9 773.1 0 9 214 4 773.1 0 4 215 38 773.1 0 38 216 18 773.1 0 18 217 318 773.1 0 318 218 18 773.1 0 18 219 9 773.1 0 9 220 12 773.1 0 12 221 2 773.1 0 2 222 2 773.1 0 2 223 6 773.1 0 6 224 4 773.1 0 4 225 14 773.1 0 14 226 7 773.1 0 7 227 13 773.1 0 13 228 19 773.1 0 19 229 8 773.1 0 8 230 4 773.1 0 4 231 8 773.1 0 8 232 4 773.1 0 4 233 16 773.1 0 16 234 27 773.1 0 27 235 20 773.1 0 20 236 24 773.1 0 24 237 13 773.1 0 13 238 8 773.1 0 8 239 8 773.1 0 8 240 8 773.1 0 8 241 7 773.1 0 7 242 16 773.1 0 16 243 11 773.1 0 11 244 15 773.1 0 15 245 8 773.1 0 8 246 10 773.1 0 10 247 14 773.1 0 14 248 14 773.1 0 14 249 14 773.1 0 14 250 19 773.1 0 19 251 49 773.1 0 49 252 153 773.1 0 153 253 135 773.1 0 135 254 105 773.1 0 105 255 24 773.1 0 24 256 14 773.1 0 14 257 24 773.1 0 24 258 265 773.1 0 265 259 333 773.1 0 333 260 225 773.1 0 225 261 400 773.1 0 400 262 238 773.1 0 238 263 265 773.1 0 265 264 210 773.1 0 210 265 133 773.1 0 133 266 39 773.1 0 39 267 14 773.1 0 14 268 14 773.1 0 14 269 3 773.1 0 3 270 8 773.1 0 8 271 23 773.1 0 23 272 31 773.1 0 31 273 36 773.1 0 36 274 21 773.1 0 21 275 20 773.1 0 20 276 8 773.1 0 8 277 26 773.1 0 26 278 27 773.1 0 27 279 29 773.1 0 29 280 31 773.1 0 31 281 21 773.1 0 21 282 15 773.1 0 15 283 80 773.1 0 80 284 573 773.1 0 573 285 403 773.1 0 403 286 761 773.1 0 761 287 31 773.1 0 31 288 27 773.1 0 27 289 23 773.1 0 23 290 27 773.1 0 27 291 27 773.1 0 27 292 17 773.1 0 17 293 6 773.1 0 6 294 12 773.1 0 12 295 31 773.1 0 31 296 51 773.1 0 51 297 38 773.1 0 38 298 20 773.1 0 20 299 10 773.1 0 10 300 4 773.1 0 4 301 38 773.1 0 38 Finished in 28.56 s (72 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 397,997 Reads with adapters: 178,607 (44.9%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 178,564 (44.9%) Total basepairs processed: 113,399,937 bp Total written (filtered): 49,279,611 bp (43.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 178607 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 22 0.0 3 0 0 22 16 855 0.0 3 0 835 20 17 176277 0.0 3 173049 3103 107 18 18 411 0.0 3 64 322 10 15 19 67 0.0 3 1 1 59 6 20 889 0.0 3 739 12 0 138 21 6 0.0 3 1 0 0 5 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 1 26 1 0.0 3 0 0 1 29 2 0.0 3 2 30 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 1 61 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 5 0.0 3 5 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 0 7 194 4 0.0 3 0 0 0 4 200 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 1 1 236 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 6 0.0 3 0 0 0 6 280 7 0.0 3 0 0 0 7 281 7 0.0 3 0 0 0 7 282 6 0.0 3 0 0 0 6 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 10.06 s (56 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 178,564 Reads with adapters: 1,132 (0.6%) Reads written (passing filters): 178,564 (100.0%) Total basepairs processed: 49,279,611 bp Total written (filtered): 49,275,665 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1132 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.6% C: 6.4% G: 43.1% T: 4.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 613 2790.1 0 613 4 500 697.5 0 500 5 14 174.4 0 14 6 2 43.6 0 2 7 1 10.9 0 1 9 2 0.7 0 0 2