This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 151.57 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 220,043 (6.9%) Reads that were too short: 11,299 (0.4%) Reads written (passing filters): 208,744 (6.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 60,240,832 bp (6.3%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 220043 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1631 773.1 0 1631 7 107207 773.1 0 107207 8 89439 773.1 0 89439 9 63 773.1 0 63 10 15 773.1 0 15 11 7 773.1 0 7 12 4 773.1 0 4 13 14 773.1 0 14 14 17 773.1 0 17 15 15 773.1 0 15 16 14 773.1 0 14 17 13 773.1 0 13 18 57 773.1 0 57 19 65 773.1 0 65 20 62 773.1 0 62 21 7 773.1 0 7 22 10 773.1 0 10 23 19 773.1 0 19 24 14 773.1 0 14 25 12 773.1 0 12 26 28 773.1 0 28 27 35 773.1 0 35 28 44 773.1 0 44 29 32 773.1 0 32 30 17 773.1 0 17 31 13 773.1 0 13 32 11 773.1 0 11 33 41 773.1 0 41 34 27 773.1 0 27 35 41 773.1 0 41 36 196 773.1 0 196 37 166 773.1 0 166 38 602 773.1 0 602 39 50 773.1 0 50 40 16 773.1 0 16 41 16 773.1 0 16 42 13 773.1 0 13 43 199 773.1 0 199 44 383 773.1 0 383 45 944 773.1 0 944 46 6 773.1 0 6 47 11 773.1 0 11 48 12 773.1 0 12 49 21 773.1 0 21 50 18 773.1 0 18 51 8 773.1 0 8 52 20 773.1 0 20 53 12 773.1 0 12 54 13 773.1 0 13 55 28 773.1 0 28 56 19 773.1 0 19 57 48 773.1 0 48 58 14 773.1 0 14 59 13 773.1 0 13 60 7 773.1 0 7 61 18 773.1 0 18 62 13 773.1 0 13 63 23 773.1 0 23 64 17 773.1 0 17 65 12 773.1 0 12 66 10 773.1 0 10 67 14 773.1 0 14 68 13 773.1 0 13 69 23 773.1 0 23 70 11 773.1 0 11 71 26 773.1 0 26 72 42 773.1 0 42 73 25 773.1 0 25 74 26 773.1 0 26 75 16 773.1 0 16 76 12 773.1 0 12 77 23 773.1 0 23 78 20 773.1 0 20 79 40 773.1 0 40 80 26 773.1 0 26 81 20 773.1 0 20 82 33 773.1 0 33 83 33 773.1 0 33 84 19 773.1 0 19 85 27 773.1 0 27 86 27 773.1 0 27 87 23 773.1 0 23 88 23 773.1 0 23 89 22 773.1 0 22 90 21 773.1 0 21 91 12 773.1 0 12 92 11 773.1 0 11 93 14 773.1 0 14 94 19 773.1 0 19 95 23 773.1 0 23 96 37 773.1 0 37 97 36 773.1 0 36 98 22 773.1 0 22 99 13 773.1 0 13 100 6 773.1 0 6 101 21 773.1 0 21 102 17 773.1 0 17 103 12 773.1 0 12 104 21 773.1 0 21 105 20 773.1 0 20 106 21 773.1 0 21 107 26 773.1 0 26 108 28 773.1 0 28 109 16 773.1 0 16 110 13 773.1 0 13 111 18 773.1 0 18 112 27 773.1 0 27 113 33 773.1 0 33 114 35 773.1 0 35 115 33 773.1 0 33 116 23 773.1 0 23 117 7 773.1 0 7 118 25 773.1 0 25 119 23 773.1 0 23 120 26 773.1 0 26 121 27 773.1 0 27 122 66 773.1 0 66 123 22 773.1 0 22 124 19 773.1 0 19 125 33 773.1 0 33 126 122 773.1 0 122 127 125 773.1 0 125 128 190 773.1 0 190 129 76 773.1 0 76 130 80 773.1 0 80 131 130 773.1 0 130 132 129 773.1 0 129 133 251 773.1 0 251 134 50 773.1 0 50 135 339 773.1 0 339 136 141 773.1 0 141 137 297 773.1 0 297 138 34 773.1 0 34 139 60 773.1 0 60 140 124 773.1 0 124 141 165 773.1 0 165 142 165 773.1 0 165 143 53 773.1 0 53 144 30 773.1 0 30 145 47 773.1 0 47 146 44 773.1 0 44 147 64 773.1 0 64 148 157 773.1 0 157 149 235 773.1 0 235 150 180 773.1 0 180 151 35 773.1 0 35 152 35 773.1 0 35 153 24 773.1 0 24 154 34 773.1 0 34 155 43 773.1 0 43 156 23 773.1 0 23 157 28 773.1 0 28 158 16 773.1 0 16 159 38 773.1 0 38 160 78 773.1 0 78 161 46 773.1 0 46 162 36 773.1 0 36 163 35 773.1 0 35 164 25 773.1 0 25 165 36 773.1 0 36 166 54 773.1 0 54 167 67 773.1 0 67 168 37 773.1 0 37 169 89 773.1 0 89 170 25 773.1 0 25 171 19 773.1 0 19 172 34 773.1 0 34 173 27 773.1 0 27 174 27 773.1 0 27 175 45 773.1 0 45 176 32 773.1 0 32 177 35 773.1 0 35 178 41 773.1 0 41 179 34 773.1 0 34 180 42 773.1 0 42 181 46 773.1 0 46 182 85 773.1 0 85 183 54 773.1 0 54 184 58 773.1 0 58 185 52 773.1 0 52 186 44 773.1 0 44 187 47 773.1 0 47 188 35 773.1 0 35 189 140 773.1 0 140 190 45 773.1 0 45 191 54 773.1 0 54 192 35 773.1 0 35 193 31 773.1 0 31 194 69 773.1 0 69 195 44 773.1 0 44 196 53 773.1 0 53 197 18 773.1 0 18 198 28 773.1 0 28 199 22 773.1 0 22 200 33 773.1 0 33 201 51 773.1 0 51 202 47 773.1 0 47 203 28 773.1 0 28 204 24 773.1 0 24 205 28 773.1 0 28 206 30 773.1 0 30 207 95 773.1 0 95 208 168 773.1 0 168 209 239 773.1 0 239 210 99 773.1 0 99 211 66 773.1 0 66 212 46 773.1 0 46 213 113 773.1 0 113 214 183 773.1 0 183 215 196 773.1 0 196 216 118 773.1 0 118 217 62 773.1 0 62 218 36 773.1 0 36 219 60 773.1 0 60 220 63 773.1 0 63 221 80 773.1 0 80 222 52 773.1 0 52 223 40 773.1 0 40 224 38 773.1 0 38 225 52 773.1 0 52 226 54 773.1 0 54 227 48 773.1 0 48 228 75 773.1 0 75 229 67 773.1 0 67 230 116 773.1 0 116 231 59 773.1 0 59 232 59 773.1 0 59 233 103 773.1 0 103 234 112 773.1 0 112 235 120 773.1 0 120 236 77 773.1 0 77 237 36 773.1 0 36 238 31 773.1 0 31 239 51 773.1 0 51 240 44 773.1 0 44 241 41 773.1 0 41 242 59 773.1 0 59 243 60 773.1 0 60 244 73 773.1 0 73 245 56 773.1 0 56 246 46 773.1 0 46 247 39 773.1 0 39 248 43 773.1 0 43 249 43 773.1 0 43 250 39 773.1 0 39 251 39 773.1 0 39 252 33 773.1 0 33 253 47 773.1 0 47 254 55 773.1 0 55 255 44 773.1 0 44 256 56 773.1 0 56 257 61 773.1 0 61 258 67 773.1 0 67 259 74 773.1 0 74 260 79 773.1 0 79 261 78 773.1 0 78 262 61 773.1 0 61 263 47 773.1 0 47 264 53 773.1 0 53 265 56 773.1 0 56 266 50 773.1 0 50 267 59 773.1 0 59 268 99 773.1 0 99 269 243 773.1 0 243 270 144 773.1 0 144 271 277 773.1 0 277 272 183 773.1 0 183 273 170 773.1 0 170 274 229 773.1 0 229 275 133 773.1 0 133 276 94 773.1 0 94 277 91 773.1 0 91 278 44 773.1 0 44 279 129 773.1 0 129 280 171 773.1 0 171 281 350 773.1 0 350 282 142 773.1 0 142 283 96 773.1 0 96 284 66 773.1 0 66 285 75 773.1 0 75 286 78 773.1 0 78 287 60 773.1 0 60 288 83 773.1 0 83 289 90 773.1 0 90 290 90 773.1 0 90 291 87 773.1 0 87 292 88 773.1 0 88 293 166 773.1 0 166 294 498 773.1 0 498 295 839 773.1 0 839 296 765 773.1 0 765 297 463 773.1 0 463 298 165 773.1 0 165 299 82 773.1 0 82 300 160 773.1 0 160 301 376 773.1 0 376 Finished in 16.72 s (80 us/read; 0.75 M reads/minute). === Summary === Total reads processed: 208,744 Reads with adapters: 92,847 (44.5%) Reads that were too short: 52 (0.0%) Reads written (passing filters): 92,795 (44.5%) Total basepairs processed: 60,240,832 bp Total written (filtered): 25,280,797 bp (42.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 92847 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 11 0.0 3 0 0 0 5 6 18 38 0.0 3 0 0 30 5 3 19 1128 0.0 3 0 952 85 59 32 20 90632 0.0 4 85975 3702 691 194 70 21 214 0.0 4 30 174 8 2 22 20 0.0 4 1 0 13 3 3 23 218 0.0 4 2 0 1 165 50 24 6 0.0 4 0 0 0 0 6 25 1 0.0 4 0 1 29 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 1 0.0 4 1 56 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 1 0.0 4 1 85 1 0.0 4 0 0 0 0 1 91 20 0.0 4 0 0 0 0 20 94 2 0.0 4 0 0 0 1 1 98 11 0.0 4 0 0 0 0 11 106 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 459 0.0 4 0 0 0 0 459 129 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 11 0.0 4 0 0 0 1 10 174 2 0.0 4 0 0 0 0 2 189 2 0.0 4 0 0 0 0 2 193 2 0.0 4 0 0 0 1 1 198 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 225 3 0.0 4 0 0 0 0 3 228 8 0.0 4 0 0 0 1 7 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 4 0.0 4 0 0 0 0 4 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 3 0.0 4 0 0 0 0 3 263 2 0.0 4 0 0 0 0 2 264 2 0.0 4 0 0 0 0 2 265 2 0.0 4 0 0 0 0 2 282 3 0.0 4 0 0 0 0 3 285 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.58 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 92,795 Reads with adapters: 14,281 (15.4%) Reads written (passing filters): 92,795 (100.0%) Total basepairs processed: 25,280,797 bp Total written (filtered): 24,877,591 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14281 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.1% C: 7.1% G: 84.2% T: 4.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 112 1449.9 0 112 4 37 362.5 0 37 5 1 90.6 0 1 7 4 5.7 0 4 8 6 1.4 0 6 9 127 0.4 0 8 119 10 4083 0.1 1 261 3822 11 8756 0.0 1 214 8542 12 114 0.0 1 5 109 13 13 0.0 1 0 13 14 11 0.0 1 1 10 15 4 0.0 1 0 4 18 9 0.0 1 7 2 127 1 0.0 1 1 151 4 0.0 1 2 2 153 1 0.0 1 1 190 3 0.0 1 0 3 209 34 0.0 1 29 5 210 1 0.0 1 1 211 1 0.0 1 1 224 29 0.0 1 28 1 230 17 0.0 1 14 3 231 1 0.0 1 1 259 245 0.0 1 232 13 260 17 0.0 1 14 3 261 1 0.0 1 1 262 1 0.0 1 0 1 268 4 0.0 1 4 269 533 0.0 1 487 46 270 107 0.0 1 98 9 271 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.