This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.37 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 215,903 (6.8%) Reads that were too short: 5,902 (0.2%) Reads written (passing filters): 210,001 (6.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 60,880,754 bp (6.4%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 215903 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1199 773.1 0 1199 7 95909 773.1 0 95909 8 105712 773.1 0 105712 9 70 773.1 0 70 10 12 773.1 0 12 11 8 773.1 0 8 12 9 773.1 0 9 13 9 773.1 0 9 14 11 773.1 0 11 15 18 773.1 0 18 16 17 773.1 0 17 17 16 773.1 0 16 18 26 773.1 0 26 19 21 773.1 0 21 20 16 773.1 0 16 21 17 773.1 0 17 22 18 773.1 0 18 23 30 773.1 0 30 24 19 773.1 0 19 25 26 773.1 0 26 26 14 773.1 0 14 27 26 773.1 0 26 28 12 773.1 0 12 29 15 773.1 0 15 30 20 773.1 0 20 31 15 773.1 0 15 32 10 773.1 0 10 33 15 773.1 0 15 34 14 773.1 0 14 35 17 773.1 0 17 36 132 773.1 0 132 37 128 773.1 0 128 38 218 773.1 0 218 39 24 773.1 0 24 40 16 773.1 0 16 41 28 773.1 0 28 42 17 773.1 0 17 43 52 773.1 0 52 44 122 773.1 0 122 45 234 773.1 0 234 46 13 773.1 0 13 47 14 773.1 0 14 48 17 773.1 0 17 49 27 773.1 0 27 50 23 773.1 0 23 51 9 773.1 0 9 52 26 773.1 0 26 53 20 773.1 0 20 54 14 773.1 0 14 55 22 773.1 0 22 56 37 773.1 0 37 57 23 773.1 0 23 58 27 773.1 0 27 59 31 773.1 0 31 60 14 773.1 0 14 61 23 773.1 0 23 62 16 773.1 0 16 63 23 773.1 0 23 64 9 773.1 0 9 65 14 773.1 0 14 66 6 773.1 0 6 67 9 773.1 0 9 68 9 773.1 0 9 69 15 773.1 0 15 70 7 773.1 0 7 71 10 773.1 0 10 72 47 773.1 0 47 73 16 773.1 0 16 74 16 773.1 0 16 75 23 773.1 0 23 76 26 773.1 0 26 77 22 773.1 0 22 78 29 773.1 0 29 79 22 773.1 0 22 80 15 773.1 0 15 81 13 773.1 0 13 82 26 773.1 0 26 83 15 773.1 0 15 84 28 773.1 0 28 85 17 773.1 0 17 86 19 773.1 0 19 87 35 773.1 0 35 88 25 773.1 0 25 89 28 773.1 0 28 90 24 773.1 0 24 91 20 773.1 0 20 92 30 773.1 0 30 93 24 773.1 0 24 94 29 773.1 0 29 95 26 773.1 0 26 96 50 773.1 0 50 97 23 773.1 0 23 98 19 773.1 0 19 99 18 773.1 0 18 100 17 773.1 0 17 101 12 773.1 0 12 102 16 773.1 0 16 103 16 773.1 0 16 104 27 773.1 0 27 105 16 773.1 0 16 106 18 773.1 0 18 107 9 773.1 0 9 108 31 773.1 0 31 109 16 773.1 0 16 110 9 773.1 0 9 111 14 773.1 0 14 112 15 773.1 0 15 113 16 773.1 0 16 114 24 773.1 0 24 115 33 773.1 0 33 116 17 773.1 0 17 117 13 773.1 0 13 118 25 773.1 0 25 119 14 773.1 0 14 120 30 773.1 0 30 121 28 773.1 0 28 122 43 773.1 0 43 123 28 773.1 0 28 124 16 773.1 0 16 125 24 773.1 0 24 126 71 773.1 0 71 127 88 773.1 0 88 128 72 773.1 0 72 129 27 773.1 0 27 130 50 773.1 0 50 131 191 773.1 0 191 132 149 773.1 0 149 133 275 773.1 0 275 134 78 773.1 0 78 135 324 773.1 0 324 136 147 773.1 0 147 137 216 773.1 0 216 138 32 773.1 0 32 139 58 773.1 0 58 140 94 773.1 0 94 141 130 773.1 0 130 142 138 773.1 0 138 143 38 773.1 0 38 144 18 773.1 0 18 145 28 773.1 0 28 146 28 773.1 0 28 147 54 773.1 0 54 148 88 773.1 0 88 149 120 773.1 0 120 150 108 773.1 0 108 151 35 773.1 0 35 152 30 773.1 0 30 153 24 773.1 0 24 154 24 773.1 0 24 155 22 773.1 0 22 156 14 773.1 0 14 157 18 773.1 0 18 158 19 773.1 0 19 159 21 773.1 0 21 160 54 773.1 0 54 161 26 773.1 0 26 162 17 773.1 0 17 163 23 773.1 0 23 164 19 773.1 0 19 165 17 773.1 0 17 166 21 773.1 0 21 167 25 773.1 0 25 168 15 773.1 0 15 169 71 773.1 0 71 170 13 773.1 0 13 171 25 773.1 0 25 172 21 773.1 0 21 173 26 773.1 0 26 174 26 773.1 0 26 175 16 773.1 0 16 176 29 773.1 0 29 177 22 773.1 0 22 178 15 773.1 0 15 179 22 773.1 0 22 180 33 773.1 0 33 181 28 773.1 0 28 182 24 773.1 0 24 183 23 773.1 0 23 184 31 773.1 0 31 185 29 773.1 0 29 186 27 773.1 0 27 187 28 773.1 0 28 188 28 773.1 0 28 189 135 773.1 0 135 190 21 773.1 0 21 191 26 773.1 0 26 192 25 773.1 0 25 193 20 773.1 0 20 194 52 773.1 0 52 195 39 773.1 0 39 196 29 773.1 0 29 197 12 773.1 0 12 198 22 773.1 0 22 199 31 773.1 0 31 200 17 773.1 0 17 201 27 773.1 0 27 202 39 773.1 0 39 203 28 773.1 0 28 204 27 773.1 0 27 205 21 773.1 0 21 206 13 773.1 0 13 207 53 773.1 0 53 208 59 773.1 0 59 209 78 773.1 0 78 210 44 773.1 0 44 211 45 773.1 0 45 212 34 773.1 0 34 213 39 773.1 0 39 214 74 773.1 0 74 215 79 773.1 0 79 216 62 773.1 0 62 217 35 773.1 0 35 218 17 773.1 0 17 219 39 773.1 0 39 220 32 773.1 0 32 221 35 773.1 0 35 222 32 773.1 0 32 223 24 773.1 0 24 224 19 773.1 0 19 225 29 773.1 0 29 226 35 773.1 0 35 227 24 773.1 0 24 228 33 773.1 0 33 229 40 773.1 0 40 230 44 773.1 0 44 231 29 773.1 0 29 232 27 773.1 0 27 233 44 773.1 0 44 234 45 773.1 0 45 235 34 773.1 0 34 236 47 773.1 0 47 237 21 773.1 0 21 238 30 773.1 0 30 239 29 773.1 0 29 240 22 773.1 0 22 241 19 773.1 0 19 242 41 773.1 0 41 243 30 773.1 0 30 244 32 773.1 0 32 245 24 773.1 0 24 246 27 773.1 0 27 247 21 773.1 0 21 248 19 773.1 0 19 249 25 773.1 0 25 250 12 773.1 0 12 251 27 773.1 0 27 252 23 773.1 0 23 253 36 773.1 0 36 254 42 773.1 0 42 255 35 773.1 0 35 256 25 773.1 0 25 257 24 773.1 0 24 258 35 773.1 0 35 259 29 773.1 0 29 260 39 773.1 0 39 261 44 773.1 0 44 262 33 773.1 0 33 263 32 773.1 0 32 264 35 773.1 0 35 265 23 773.1 0 23 266 31 773.1 0 31 267 32 773.1 0 32 268 42 773.1 0 42 269 125 773.1 0 125 270 91 773.1 0 91 271 142 773.1 0 142 272 64 773.1 0 64 273 52 773.1 0 52 274 89 773.1 0 89 275 67 773.1 0 67 276 54 773.1 0 54 277 38 773.1 0 38 278 31 773.1 0 31 279 65 773.1 0 65 280 105 773.1 0 105 281 191 773.1 0 191 282 53 773.1 0 53 283 41 773.1 0 41 284 32 773.1 0 32 285 46 773.1 0 46 286 46 773.1 0 46 287 43 773.1 0 43 288 44 773.1 0 44 289 35 773.1 0 35 290 42 773.1 0 42 291 73 773.1 0 73 292 51 773.1 0 51 293 67 773.1 0 67 294 274 773.1 0 274 295 531 773.1 0 531 296 432 773.1 0 432 297 223 773.1 0 223 298 81 773.1 0 81 299 38 773.1 0 38 300 95 773.1 0 95 301 243 773.1 0 243 Finished in 14.93 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 210,001 Reads with adapters: 94,296 (44.9%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 94,262 (44.9%) Total basepairs processed: 60,880,754 bp Total written (filtered): 26,106,001 bp (42.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 94296 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 10 0.0 3 0 0 10 16 520 0.0 3 0 504 14 2 17 93403 0.0 3 91709 1638 53 3 18 189 0.0 3 8 169 10 2 19 34 0.0 3 0 0 30 4 20 70 0.0 3 0 0 0 70 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 26 1 0.0 3 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 52 7 0.0 3 0 0 0 7 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 65 3 0.0 3 0 1 0 2 68 1 0.0 3 0 0 0 1 75 1 0.0 3 1 92 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 128 3 0.0 3 0 0 0 3 135 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 6 0.0 3 0 0 0 6 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.23 s (55 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 94,262 Reads with adapters: 821 (0.9%) Reads written (passing filters): 94,262 (100.0%) Total basepairs processed: 26,106,001 bp Total written (filtered): 26,103,491 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 821 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.2% C: 8.4% G: 14.4% T: 48.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 779 1472.8 0 779 4 38 368.2 0 38 5 3 92.1 0 3 6 1 23.0 0 1