This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 146.20 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 365,884 (11.6%) Reads that were too short: 11,936 (0.4%) Reads written (passing filters): 353,948 (11.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 101,259,026 bp (10.6%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 365884 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 7931 773.1 0 7931 7 143502 773.1 0 143502 8 2715 773.1 0 2715 9 176893 773.1 0 176893 10 110 773.1 0 110 11 61 773.1 0 61 12 24 773.1 0 24 13 64 773.1 0 64 14 55 773.1 0 55 15 42 773.1 0 42 16 53 773.1 0 53 17 80 773.1 0 80 18 45 773.1 0 45 19 54 773.1 0 54 20 31 773.1 0 31 21 40 773.1 0 40 22 41 773.1 0 41 23 62 773.1 0 62 24 33 773.1 0 33 25 25 773.1 0 25 26 44 773.1 0 44 27 48 773.1 0 48 28 57 773.1 0 57 29 52 773.1 0 52 30 50 773.1 0 50 31 49 773.1 0 49 32 36 773.1 0 36 33 28 773.1 0 28 34 54 773.1 0 54 35 49 773.1 0 49 36 41 773.1 0 41 37 52 773.1 0 52 38 40 773.1 0 40 39 54 773.1 0 54 40 53 773.1 0 53 41 57 773.1 0 57 42 159 773.1 0 159 43 148 773.1 0 148 44 134 773.1 0 134 45 33 773.1 0 33 46 41 773.1 0 41 47 45 773.1 0 45 48 55 773.1 0 55 49 57 773.1 0 57 50 76 773.1 0 76 51 49 773.1 0 49 52 57 773.1 0 57 53 34 773.1 0 34 54 46 773.1 0 46 55 58 773.1 0 58 56 40 773.1 0 40 57 38 773.1 0 38 58 61 773.1 0 61 59 50 773.1 0 50 60 24 773.1 0 24 61 40 773.1 0 40 62 55 773.1 0 55 63 32 773.1 0 32 64 43 773.1 0 43 65 85 773.1 0 85 66 75 773.1 0 75 67 59 773.1 0 59 68 31 773.1 0 31 69 46 773.1 0 46 70 37 773.1 0 37 71 42 773.1 0 42 72 47 773.1 0 47 73 76 773.1 0 76 74 62 773.1 0 62 75 153 773.1 0 153 76 98 773.1 0 98 77 103 773.1 0 103 78 46 773.1 0 46 79 128 773.1 0 128 80 135 773.1 0 135 81 67 773.1 0 67 82 43 773.1 0 43 83 35 773.1 0 35 84 36 773.1 0 36 85 55 773.1 0 55 86 60 773.1 0 60 87 62 773.1 0 62 88 54 773.1 0 54 89 59 773.1 0 59 90 97 773.1 0 97 91 99 773.1 0 99 92 872 773.1 0 872 93 1404 773.1 0 1404 94 4347 773.1 0 4347 95 56 773.1 0 56 96 41 773.1 0 41 97 37 773.1 0 37 98 44 773.1 0 44 99 47 773.1 0 47 100 43 773.1 0 43 101 38 773.1 0 38 102 35 773.1 0 35 103 22 773.1 0 22 104 43 773.1 0 43 105 59 773.1 0 59 106 51 773.1 0 51 107 46 773.1 0 46 108 58 773.1 0 58 109 57 773.1 0 57 110 68 773.1 0 68 111 48 773.1 0 48 112 49 773.1 0 49 113 90 773.1 0 90 114 93 773.1 0 93 115 41 773.1 0 41 116 63 773.1 0 63 117 75 773.1 0 75 118 70 773.1 0 70 119 84 773.1 0 84 120 65 773.1 0 65 121 84 773.1 0 84 122 56 773.1 0 56 123 438 773.1 0 438 124 1337 773.1 0 1337 125 601 773.1 0 601 126 87 773.1 0 87 127 60 773.1 0 60 128 150 773.1 0 150 129 128 773.1 0 128 130 273 773.1 0 273 131 299 773.1 0 299 132 286 773.1 0 286 133 35 773.1 0 35 134 47 773.1 0 47 135 48 773.1 0 48 136 155 773.1 0 155 137 51 773.1 0 51 138 56 773.1 0 56 139 42 773.1 0 42 140 39 773.1 0 39 141 36 773.1 0 36 142 37 773.1 0 37 143 42 773.1 0 42 144 41 773.1 0 41 145 37 773.1 0 37 146 84 773.1 0 84 147 68 773.1 0 68 148 73 773.1 0 73 149 31 773.1 0 31 150 32 773.1 0 32 151 45 773.1 0 45 152 26 773.1 0 26 153 32 773.1 0 32 154 39 773.1 0 39 155 54 773.1 0 54 156 38 773.1 0 38 157 23 773.1 0 23 158 39 773.1 0 39 159 47 773.1 0 47 160 41 773.1 0 41 161 35 773.1 0 35 162 52 773.1 0 52 163 53 773.1 0 53 164 49 773.1 0 49 165 39 773.1 0 39 166 49 773.1 0 49 167 96 773.1 0 96 168 63 773.1 0 63 169 61 773.1 0 61 170 33 773.1 0 33 171 47 773.1 0 47 172 54 773.1 0 54 173 140 773.1 0 140 174 75 773.1 0 75 175 70 773.1 0 70 176 86 773.1 0 86 177 60 773.1 0 60 178 235 773.1 0 235 179 305 773.1 0 305 180 387 773.1 0 387 181 86 773.1 0 86 182 44 773.1 0 44 183 60 773.1 0 60 184 54 773.1 0 54 185 129 773.1 0 129 186 83 773.1 0 83 187 44 773.1 0 44 188 79 773.1 0 79 189 62 773.1 0 62 190 49 773.1 0 49 191 39 773.1 0 39 192 66 773.1 0 66 193 94 773.1 0 94 194 111 773.1 0 111 195 143 773.1 0 143 196 127 773.1 0 127 197 91 773.1 0 91 198 334 773.1 0 334 199 402 773.1 0 402 200 397 773.1 0 397 201 392 773.1 0 392 202 125 773.1 0 125 203 101 773.1 0 101 204 63 773.1 0 63 205 103 773.1 0 103 206 109 773.1 0 109 207 136 773.1 0 136 208 90 773.1 0 90 209 73 773.1 0 73 210 60 773.1 0 60 211 57 773.1 0 57 212 47 773.1 0 47 213 67 773.1 0 67 214 46 773.1 0 46 215 90 773.1 0 90 216 77 773.1 0 77 217 84 773.1 0 84 218 100 773.1 0 100 219 284 773.1 0 284 220 228 773.1 0 228 221 249 773.1 0 249 222 351 773.1 0 351 223 208 773.1 0 208 224 320 773.1 0 320 225 81 773.1 0 81 226 77 773.1 0 77 227 68 773.1 0 68 228 136 773.1 0 136 229 69 773.1 0 69 230 71 773.1 0 71 231 37 773.1 0 37 232 54 773.1 0 54 233 36 773.1 0 36 234 48 773.1 0 48 235 48 773.1 0 48 236 73 773.1 0 73 237 44 773.1 0 44 238 210 773.1 0 210 239 242 773.1 0 242 240 586 773.1 0 586 241 547 773.1 0 547 242 911 773.1 0 911 243 62 773.1 0 62 244 59 773.1 0 59 245 71 773.1 0 71 246 66 773.1 0 66 247 89 773.1 0 89 248 117 773.1 0 117 249 73 773.1 0 73 250 92 773.1 0 92 251 162 773.1 0 162 252 118 773.1 0 118 253 92 773.1 0 92 254 75 773.1 0 75 255 77 773.1 0 77 256 61 773.1 0 61 257 70 773.1 0 70 258 50 773.1 0 50 259 44 773.1 0 44 260 63 773.1 0 63 261 114 773.1 0 114 262 63 773.1 0 63 263 85 773.1 0 85 264 66 773.1 0 66 265 51 773.1 0 51 266 52 773.1 0 52 267 57 773.1 0 57 268 43 773.1 0 43 269 53 773.1 0 53 270 67 773.1 0 67 271 57 773.1 0 57 272 66 773.1 0 66 273 46 773.1 0 46 274 40 773.1 0 40 275 53 773.1 0 53 276 43 773.1 0 43 277 80 773.1 0 80 278 88 773.1 0 88 279 99 773.1 0 99 280 53 773.1 0 53 281 57 773.1 0 57 282 53 773.1 0 53 283 94 773.1 0 94 284 121 773.1 0 121 285 89 773.1 0 89 286 124 773.1 0 124 287 107 773.1 0 107 288 366 773.1 0 366 289 325 773.1 0 325 290 403 773.1 0 403 291 171 773.1 0 171 292 110 773.1 0 110 293 60 773.1 0 60 294 54 773.1 0 54 295 60 773.1 0 60 296 71 773.1 0 71 297 77 773.1 0 77 298 63 773.1 0 63 299 153 773.1 0 153 300 161 773.1 0 161 301 94 773.1 0 94 Finished in 26.28 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 353,948 Reads with adapters: 149,453 (42.2%) Reads that were too short: 95 (0.0%) Reads written (passing filters): 149,358 (42.2%) Total basepairs processed: 101,259,026 bp Total written (filtered): 40,894,490 bp (40.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 149453 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 6 0.0 3 0 0 0 4 2 18 64 0.0 3 0 0 45 16 3 19 1625 0.0 3 0 1392 143 67 23 20 146812 0.0 4 140338 5397 819 214 44 21 271 0.0 4 31 222 11 5 2 22 13 0.0 4 2 0 11 23 232 0.0 4 0 0 1 186 45 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 34 1 0.0 4 0 0 1 38 1 0.0 4 1 43 1 0.0 4 0 0 0 1 44 1 0.0 4 0 0 0 1 47 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 86 23 0.0 4 0 0 0 0 23 90 1 0.0 4 0 0 0 0 1 92 4 0.0 4 0 0 0 0 4 112 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 241 0.0 4 0 0 0 0 241 128 3 0.0 4 0 0 0 0 3 129 2 0.0 4 0 0 0 0 2 130 25 0.0 4 0 0 0 0 25 137 1 0.0 4 0 0 0 0 1 140 2 0.0 4 1 0 0 0 1 145 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 0 2 153 10 0.0 4 0 0 0 0 10 158 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 194 7 0.0 4 0 0 0 1 6 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 0 8 229 10 0.0 4 0 0 0 1 9 230 4 0.0 4 0 0 0 0 4 231 2 0.0 4 0 0 0 0 2 232 2 0.0 4 0 0 0 0 2 233 5 0.0 4 0 0 0 0 5 234 2 0.0 4 0 0 0 0 2 235 5 0.0 4 0 0 0 1 4 236 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 1 254 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 6 0.0 4 0 0 0 0 6 263 2 0.0 4 0 0 0 0 2 265 3 0.0 4 0 0 0 0 3 267 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 280 2 0.0 4 0 0 0 0 2 281 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 283 3 0.0 4 0 0 0 1 2 287 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.66 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 149,358 Reads with adapters: 14,602 (9.8%) Reads written (passing filters): 149,358 (100.0%) Total basepairs processed: 40,894,490 bp Total written (filtered): 40,633,196 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14602 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.7% C: 5.5% G: 87.1% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 364 2333.7 0 364 4 72 583.4 0 72 5 8 145.9 0 8 6 1 36.5 0 1 7 3 9.1 0 3 8 4 2.3 0 4 9 33 0.6 0 2 31 10 148 0.1 1 4 144 11 12632 0.0 1 226 12406 12 709 0.0 1 31 678 13 62 0.0 1 5 57 14 4 0.0 1 0 4 15 23 0.0 1 7 16 16 4 0.0 1 1 3 26 1 0.0 1 0 1 29 1 0.0 1 0 1 46 1 0.0 1 0 1 57 1 0.0 1 0 1 58 1 0.0 1 1 72 1 0.0 1 1 81 1 0.0 1 1 84 1 0.0 1 1 124 1 0.0 1 1 149 4 0.0 1 3 1 152 15 0.0 1 9 6 171 1 0.0 1 1 172 1 0.0 1 0 1 191 2 0.0 1 0 2 203 2 0.0 1 2 205 1 0.0 1 1 206 262 0.0 1 245 17 207 16 0.0 1 14 2 208 4 0.0 1 4 209 2 0.0 1 1 1 210 174 0.0 1 148 26 211 15 0.0 1 13 2 225 20 0.0 1 17 3 228 2 0.0 1 2 230 1 0.0 1 1 273 3 0.0 1 3 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.