This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 148.96 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 368,316 (11.6%) Reads that were too short: 8,161 (0.3%) Reads written (passing filters): 360,155 (11.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 103,725,337 bp (10.9%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 368316 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 7444 773.1 0 7444 7 181049 773.1 0 181049 8 1663 773.1 0 1663 9 152009 773.1 0 152009 10 84 773.1 0 84 11 24 773.1 0 24 12 23 773.1 0 23 13 35 773.1 0 35 14 27 773.1 0 27 15 30 773.1 0 30 16 34 773.1 0 34 17 30 773.1 0 30 18 24 773.1 0 24 19 32 773.1 0 32 20 17 773.1 0 17 21 26 773.1 0 26 22 29 773.1 0 29 23 38 773.1 0 38 24 23 773.1 0 23 25 16 773.1 0 16 26 30 773.1 0 30 27 29 773.1 0 29 28 33 773.1 0 33 29 33 773.1 0 33 30 28 773.1 0 28 31 37 773.1 0 37 32 30 773.1 0 30 33 25 773.1 0 25 34 26 773.1 0 26 35 37 773.1 0 37 36 18 773.1 0 18 37 27 773.1 0 27 38 29 773.1 0 29 39 30 773.1 0 30 40 32 773.1 0 32 41 46 773.1 0 46 42 78 773.1 0 78 43 94 773.1 0 94 44 69 773.1 0 69 45 26 773.1 0 26 46 16 773.1 0 16 47 20 773.1 0 20 48 27 773.1 0 27 49 25 773.1 0 25 50 40 773.1 0 40 51 27 773.1 0 27 52 25 773.1 0 25 53 13 773.1 0 13 54 34 773.1 0 34 55 40 773.1 0 40 56 35 773.1 0 35 57 27 773.1 0 27 58 43 773.1 0 43 59 27 773.1 0 27 60 12 773.1 0 12 61 29 773.1 0 29 62 39 773.1 0 39 63 29 773.1 0 29 64 29 773.1 0 29 65 51 773.1 0 51 66 52 773.1 0 52 67 30 773.1 0 30 68 33 773.1 0 33 69 27 773.1 0 27 70 31 773.1 0 31 71 29 773.1 0 29 72 28 773.1 0 28 73 37 773.1 0 37 74 58 773.1 0 58 75 71 773.1 0 71 76 56 773.1 0 56 77 59 773.1 0 59 78 21 773.1 0 21 79 93 773.1 0 93 80 73 773.1 0 73 81 48 773.1 0 48 82 15 773.1 0 15 83 26 773.1 0 26 84 17 773.1 0 17 85 31 773.1 0 31 86 30 773.1 0 30 87 41 773.1 0 41 88 47 773.1 0 47 89 56 773.1 0 56 90 84 773.1 0 84 91 77 773.1 0 77 92 781 773.1 0 781 93 1533 773.1 0 1533 94 4613 773.1 0 4613 95 52 773.1 0 52 96 32 773.1 0 32 97 36 773.1 0 36 98 34 773.1 0 34 99 32 773.1 0 32 100 19 773.1 0 19 101 32 773.1 0 32 102 29 773.1 0 29 103 19 773.1 0 19 104 27 773.1 0 27 105 35 773.1 0 35 106 29 773.1 0 29 107 38 773.1 0 38 108 56 773.1 0 56 109 36 773.1 0 36 110 46 773.1 0 46 111 37 773.1 0 37 112 38 773.1 0 38 113 73 773.1 0 73 114 77 773.1 0 77 115 35 773.1 0 35 116 36 773.1 0 36 117 47 773.1 0 47 118 43 773.1 0 43 119 46 773.1 0 46 120 54 773.1 0 54 121 49 773.1 0 49 122 46 773.1 0 46 123 378 773.1 0 378 124 1326 773.1 0 1326 125 599 773.1 0 599 126 37 773.1 0 37 127 26 773.1 0 26 128 104 773.1 0 104 129 88 773.1 0 88 130 144 773.1 0 144 131 179 773.1 0 179 132 161 773.1 0 161 133 37 773.1 0 37 134 23 773.1 0 23 135 41 773.1 0 41 136 125 773.1 0 125 137 47 773.1 0 47 138 38 773.1 0 38 139 40 773.1 0 40 140 29 773.1 0 29 141 24 773.1 0 24 142 40 773.1 0 40 143 35 773.1 0 35 144 31 773.1 0 31 145 44 773.1 0 44 146 94 773.1 0 94 147 94 773.1 0 94 148 59 773.1 0 59 149 29 773.1 0 29 150 33 773.1 0 33 151 23 773.1 0 23 152 36 773.1 0 36 153 30 773.1 0 30 154 22 773.1 0 22 155 37 773.1 0 37 156 26 773.1 0 26 157 31 773.1 0 31 158 28 773.1 0 28 159 37 773.1 0 37 160 24 773.1 0 24 161 27 773.1 0 27 162 45 773.1 0 45 163 35 773.1 0 35 164 46 773.1 0 46 165 32 773.1 0 32 166 34 773.1 0 34 167 58 773.1 0 58 168 29 773.1 0 29 169 35 773.1 0 35 170 31 773.1 0 31 171 20 773.1 0 20 172 33 773.1 0 33 173 75 773.1 0 75 174 48 773.1 0 48 175 51 773.1 0 51 176 52 773.1 0 52 177 43 773.1 0 43 178 131 773.1 0 131 179 120 773.1 0 120 180 183 773.1 0 183 181 72 773.1 0 72 182 40 773.1 0 40 183 36 773.1 0 36 184 32 773.1 0 32 185 101 773.1 0 101 186 115 773.1 0 115 187 28 773.1 0 28 188 99 773.1 0 99 189 47 773.1 0 47 190 37 773.1 0 37 191 31 773.1 0 31 192 47 773.1 0 47 193 56 773.1 0 56 194 50 773.1 0 50 195 57 773.1 0 57 196 34 773.1 0 34 197 35 773.1 0 35 198 160 773.1 0 160 199 222 773.1 0 222 200 169 773.1 0 169 201 128 773.1 0 128 202 57 773.1 0 57 203 40 773.1 0 40 204 46 773.1 0 46 205 61 773.1 0 61 206 63 773.1 0 63 207 60 773.1 0 60 208 57 773.1 0 57 209 35 773.1 0 35 210 33 773.1 0 33 211 33 773.1 0 33 212 30 773.1 0 30 213 48 773.1 0 48 214 41 773.1 0 41 215 42 773.1 0 42 216 43 773.1 0 43 217 40 773.1 0 40 218 58 773.1 0 58 219 95 773.1 0 95 220 89 773.1 0 89 221 91 773.1 0 91 222 280 773.1 0 280 223 242 773.1 0 242 224 410 773.1 0 410 225 63 773.1 0 63 226 43 773.1 0 43 227 38 773.1 0 38 228 60 773.1 0 60 229 59 773.1 0 59 230 38 773.1 0 38 231 28 773.1 0 28 232 39 773.1 0 39 233 32 773.1 0 32 234 53 773.1 0 53 235 36 773.1 0 36 236 49 773.1 0 49 237 44 773.1 0 44 238 117 773.1 0 117 239 140 773.1 0 140 240 501 773.1 0 501 241 574 773.1 0 574 242 855 773.1 0 855 243 53 773.1 0 53 244 56 773.1 0 56 245 77 773.1 0 77 246 45 773.1 0 45 247 36 773.1 0 36 248 66 773.1 0 66 249 54 773.1 0 54 250 87 773.1 0 87 251 81 773.1 0 81 252 62 773.1 0 62 253 52 773.1 0 52 254 45 773.1 0 45 255 34 773.1 0 34 256 55 773.1 0 55 257 49 773.1 0 49 258 47 773.1 0 47 259 47 773.1 0 47 260 45 773.1 0 45 261 92 773.1 0 92 262 42 773.1 0 42 263 77 773.1 0 77 264 44 773.1 0 44 265 43 773.1 0 43 266 41 773.1 0 41 267 46 773.1 0 46 268 48 773.1 0 48 269 44 773.1 0 44 270 36 773.1 0 36 271 44 773.1 0 44 272 32 773.1 0 32 273 30 773.1 0 30 274 33 773.1 0 33 275 37 773.1 0 37 276 35 773.1 0 35 277 47 773.1 0 47 278 42 773.1 0 42 279 36 773.1 0 36 280 35 773.1 0 35 281 37 773.1 0 37 282 41 773.1 0 41 283 48 773.1 0 48 284 86 773.1 0 86 285 57 773.1 0 57 286 37 773.1 0 37 287 63 773.1 0 63 288 138 773.1 0 138 289 150 773.1 0 150 290 161 773.1 0 161 291 84 773.1 0 84 292 47 773.1 0 47 293 56 773.1 0 56 294 41 773.1 0 41 295 45 773.1 0 45 296 38 773.1 0 38 297 53 773.1 0 53 298 42 773.1 0 42 299 102 773.1 0 102 300 106 773.1 0 106 301 91 773.1 0 91 Finished in 25.52 s (71 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 360,155 Reads with adapters: 154,921 (43.0%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 154,901 (43.0%) Total basepairs processed: 103,725,337 bp Total written (filtered): 42,597,512 bp (41.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 154921 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 54 0.0 3 0 0 53 1 16 809 0.0 3 0 787 22 17 153339 0.0 3 150280 2963 87 9 18 226 0.0 3 8 206 11 1 19 69 0.0 3 1 1 57 10 20 362 0.0 3 298 4 0 60 22 1 0.0 3 0 0 1 23 1 0.0 3 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 1 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 1 0 1 36 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 160 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 170 1 0.0 3 0 1 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 5 0.0 3 0 0 0 5 194 3 0.0 3 0 0 0 3 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 221 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.52 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 154,901 Reads with adapters: 7,128 (4.6%) Reads written (passing filters): 154,901 (100.0%) Total basepairs processed: 42,597,512 bp Total written (filtered): 42,576,009 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 7128 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.3% C: 4.8% G: 15.9% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 7012 2420.3 0 7012 4 114 605.1 0 114 5 1 151.3 0 1 6 1 37.8 0 1