This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 146.56 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 149,773 (4.7%) Reads that were too short: 43,675 (1.4%) Reads written (passing filters): 106,098 (3.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 24,907,337 bp (2.6%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 149773 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 514 773.1 0 514 7 2256 773.1 0 2256 8 457 773.1 0 457 9 1810 773.1 0 1810 10 1327 773.1 0 1327 11 45468 773.1 0 45468 12 116 773.1 0 116 13 63 773.1 0 63 14 77 773.1 0 77 15 69 773.1 0 69 16 51 773.1 0 51 17 48 773.1 0 48 18 76 773.1 0 76 19 62 773.1 0 62 20 87 773.1 0 87 21 46 773.1 0 46 22 67 773.1 0 67 23 57 773.1 0 57 24 66 773.1 0 66 25 73 773.1 0 73 26 103 773.1 0 103 27 128 773.1 0 128 28 164 773.1 0 164 29 514 773.1 0 514 30 80 773.1 0 80 31 79 773.1 0 79 32 81 773.1 0 81 33 74 773.1 0 74 34 79 773.1 0 79 35 59 773.1 0 59 36 71 773.1 0 71 37 79 773.1 0 79 38 75 773.1 0 75 39 87 773.1 0 87 40 94 773.1 0 94 41 68 773.1 0 68 42 58 773.1 0 58 43 68 773.1 0 68 44 78 773.1 0 78 45 72 773.1 0 72 46 70 773.1 0 70 47 78 773.1 0 78 48 101 773.1 0 101 49 144 773.1 0 144 50 87 773.1 0 87 51 52 773.1 0 52 52 69 773.1 0 69 53 73 773.1 0 73 54 72 773.1 0 72 55 134 773.1 0 134 56 110 773.1 0 110 57 93 773.1 0 93 58 69 773.1 0 69 59 60 773.1 0 60 60 97 773.1 0 97 61 77 773.1 0 77 62 74 773.1 0 74 63 79 773.1 0 79 64 84 773.1 0 84 65 80 773.1 0 80 66 78 773.1 0 78 67 79 773.1 0 79 68 59 773.1 0 59 69 87 773.1 0 87 70 127 773.1 0 127 71 314 773.1 0 314 72 114 773.1 0 114 73 65 773.1 0 65 74 53 773.1 0 53 75 74 773.1 0 74 76 81 773.1 0 81 77 66 773.1 0 66 78 93 773.1 0 93 79 318 773.1 0 318 80 143 773.1 0 143 81 239 773.1 0 239 82 177 773.1 0 177 83 888 773.1 0 888 84 902 773.1 0 902 85 1060 773.1 0 1060 86 78 773.1 0 78 87 71 773.1 0 71 88 71 773.1 0 71 89 78 773.1 0 78 90 173 773.1 0 173 91 67 773.1 0 67 92 48 773.1 0 48 93 53 773.1 0 53 94 57 773.1 0 57 95 75 773.1 0 75 96 136 773.1 0 136 97 124 773.1 0 124 98 257 773.1 0 257 99 155 773.1 0 155 100 167 773.1 0 167 101 104 773.1 0 104 102 70 773.1 0 70 103 94 773.1 0 94 104 90 773.1 0 90 105 203 773.1 0 203 106 126 773.1 0 126 107 120 773.1 0 120 108 116 773.1 0 116 109 123 773.1 0 123 110 156 773.1 0 156 111 196 773.1 0 196 112 357 773.1 0 357 113 5353 773.1 0 5353 114 3227 773.1 0 3227 115 6507 773.1 0 6507 116 196 773.1 0 196 117 85 773.1 0 85 118 87 773.1 0 87 119 127 773.1 0 127 120 66 773.1 0 66 121 76 773.1 0 76 122 119 773.1 0 119 123 120 773.1 0 120 124 155 773.1 0 155 125 156 773.1 0 156 126 130 773.1 0 130 127 168 773.1 0 168 128 226 773.1 0 226 129 2260 773.1 0 2260 130 2782 773.1 0 2782 131 3775 773.1 0 3775 132 95 773.1 0 95 133 89 773.1 0 89 134 99 773.1 0 99 135 129 773.1 0 129 136 162 773.1 0 162 137 177 773.1 0 177 138 148 773.1 0 148 139 257 773.1 0 257 140 216 773.1 0 216 141 227 773.1 0 227 142 123 773.1 0 123 143 104 773.1 0 104 144 110 773.1 0 110 145 95 773.1 0 95 146 118 773.1 0 118 147 102 773.1 0 102 148 81 773.1 0 81 149 147 773.1 0 147 150 108 773.1 0 108 151 97 773.1 0 97 152 102 773.1 0 102 153 138 773.1 0 138 154 1053 773.1 0 1053 155 1346 773.1 0 1346 156 3245 773.1 0 3245 157 2130 773.1 0 2130 158 812 773.1 0 812 159 147 773.1 0 147 160 60 773.1 0 60 161 67 773.1 0 67 162 54 773.1 0 54 163 81 773.1 0 81 164 113 773.1 0 113 165 51 773.1 0 51 166 66 773.1 0 66 167 167 773.1 0 167 168 166 773.1 0 166 169 198 773.1 0 198 170 107 773.1 0 107 171 61 773.1 0 61 172 93 773.1 0 93 173 49 773.1 0 49 174 56 773.1 0 56 175 69 773.1 0 69 176 67 773.1 0 67 177 57 773.1 0 57 178 341 773.1 0 341 179 54 773.1 0 54 180 73 773.1 0 73 181 39 773.1 0 39 182 56 773.1 0 56 183 59 773.1 0 59 184 90 773.1 0 90 185 99 773.1 0 99 186 92 773.1 0 92 187 72 773.1 0 72 188 128 773.1 0 128 189 69 773.1 0 69 190 94 773.1 0 94 191 85 773.1 0 85 192 98 773.1 0 98 193 60 773.1 0 60 194 57 773.1 0 57 195 51 773.1 0 51 196 119 773.1 0 119 197 75 773.1 0 75 198 58 773.1 0 58 199 58 773.1 0 58 200 200 773.1 0 200 201 131 773.1 0 131 202 213 773.1 0 213 203 67 773.1 0 67 204 92 773.1 0 92 205 72 773.1 0 72 206 66 773.1 0 66 207 73 773.1 0 73 208 55 773.1 0 55 209 78 773.1 0 78 210 89 773.1 0 89 211 209 773.1 0 209 212 260 773.1 0 260 213 311 773.1 0 311 214 161 773.1 0 161 215 149 773.1 0 149 216 148 773.1 0 148 217 76 773.1 0 76 218 78 773.1 0 78 219 54 773.1 0 54 220 65 773.1 0 65 221 61 773.1 0 61 222 88 773.1 0 88 223 74 773.1 0 74 224 92 773.1 0 92 225 73 773.1 0 73 226 78 773.1 0 78 227 109 773.1 0 109 228 813 773.1 0 813 229 599 773.1 0 599 230 1114 773.1 0 1114 231 171 773.1 0 171 232 139 773.1 0 139 233 117 773.1 0 117 234 137 773.1 0 137 235 213 773.1 0 213 236 254 773.1 0 254 237 200 773.1 0 200 238 110 773.1 0 110 239 96 773.1 0 96 240 75 773.1 0 75 241 80 773.1 0 80 242 123 773.1 0 123 243 135 773.1 0 135 244 130 773.1 0 130 245 97 773.1 0 97 246 153 773.1 0 153 247 203 773.1 0 203 248 244 773.1 0 244 249 447 773.1 0 447 250 116 773.1 0 116 251 116 773.1 0 116 252 100 773.1 0 100 253 126 773.1 0 126 254 139 773.1 0 139 255 269 773.1 0 269 256 240 773.1 0 240 257 374 773.1 0 374 258 155 773.1 0 155 259 67 773.1 0 67 260 56 773.1 0 56 261 62 773.1 0 62 262 82 773.1 0 82 263 108 773.1 0 108 264 77 773.1 0 77 265 74 773.1 0 74 266 67 773.1 0 67 267 75 773.1 0 75 268 166 773.1 0 166 269 346 773.1 0 346 270 378 773.1 0 378 271 219 773.1 0 219 272 125 773.1 0 125 273 107 773.1 0 107 274 151 773.1 0 151 275 95 773.1 0 95 276 130 773.1 0 130 277 119 773.1 0 119 278 93 773.1 0 93 279 84 773.1 0 84 280 125 773.1 0 125 281 194 773.1 0 194 282 158 773.1 0 158 283 242 773.1 0 242 284 201 773.1 0 201 285 289 773.1 0 289 286 146 773.1 0 146 287 195 773.1 0 195 288 250 773.1 0 250 289 204 773.1 0 204 290 172 773.1 0 172 291 223 773.1 0 223 292 183 773.1 0 183 293 214 773.1 0 214 294 759 773.1 0 759 295 1558 773.1 0 1558 296 4302 773.1 0 4302 297 2606 773.1 0 2606 298 5046 773.1 0 5046 299 6986 773.1 0 6986 300 6477 773.1 0 6477 301 288 773.1 0 288 Finished in 6.86 s (65 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 106,098 Reads with adapters: 1,452 (1.4%) Reads that were too short: 45 (0.0%) Reads written (passing filters): 1,407 (1.3%) Total basepairs processed: 24,907,337 bp Total written (filtered): 379,634 bp (1.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1452 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 1 19 18 0.0 3 0 18 20 1353 0.0 4 1286 49 15 3 21 5 0.0 4 1 1 0 2 1 22 2 0.0 4 0 0 2 23 5 0.0 4 0 0 0 3 2 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 1 51 1 0.0 4 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 1 120 1 0.0 4 0 0 0 0 1 121 14 0.0 4 0 0 0 0 14 123 2 0.0 4 0 0 0 0 2 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 169 12 0.0 4 0 0 0 0 12 170 2 0.0 4 0 0 0 0 2 174 1 0.0 4 0 0 0 0 1 176 1 0.0 4 1 178 1 0.0 4 0 0 0 1 197 1 0.0 4 1 202 1 0.0 4 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 1,407 Reads with adapters: 261 (18.6%) Reads written (passing filters): 1,407 (100.0%) Total basepairs processed: 379,634 bp Total written (filtered): 371,811 bp (97.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 261 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.8% C: 5.7% G: 92.0% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 2 5.5 0 2 9 61 0.0 0 6 55 10 136 0.0 1 5 131 11 40 0.0 1 0 40 13 1 0.0 1 0 1 72 1 0.0 1 1 269 19 0.0 1 16 3 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.