This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 148.30 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 148,932 (4.7%) Reads that were too short: 35,946 (1.1%) Reads written (passing filters): 112,986 (3.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 26,926,971 bp (2.8%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 148932 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 486 773.1 0 486 7 2037 773.1 0 2037 8 434 773.1 0 434 9 1957 773.1 0 1957 10 1080 773.1 0 1080 11 53925 773.1 0 53925 12 115 773.1 0 115 13 54 773.1 0 54 14 52 773.1 0 52 15 65 773.1 0 65 16 55 773.1 0 55 17 40 773.1 0 40 18 71 773.1 0 71 19 47 773.1 0 47 20 60 773.1 0 60 21 61 773.1 0 61 22 67 773.1 0 67 23 54 773.1 0 54 24 51 773.1 0 51 25 60 773.1 0 60 26 56 773.1 0 56 27 63 773.1 0 63 28 81 773.1 0 81 29 499 773.1 0 499 30 79 773.1 0 79 31 65 773.1 0 65 32 84 773.1 0 84 33 70 773.1 0 70 34 86 773.1 0 86 35 69 773.1 0 69 36 76 773.1 0 76 37 78 773.1 0 78 38 75 773.1 0 75 39 77 773.1 0 77 40 81 773.1 0 81 41 63 773.1 0 63 42 72 773.1 0 72 43 43 773.1 0 43 44 71 773.1 0 71 45 63 773.1 0 63 46 58 773.1 0 58 47 57 773.1 0 57 48 87 773.1 0 87 49 136 773.1 0 136 50 56 773.1 0 56 51 76 773.1 0 76 52 57 773.1 0 57 53 56 773.1 0 56 54 75 773.1 0 75 55 101 773.1 0 101 56 101 773.1 0 101 57 63 773.1 0 63 58 57 773.1 0 57 59 85 773.1 0 85 60 86 773.1 0 86 61 76 773.1 0 76 62 79 773.1 0 79 63 66 773.1 0 66 64 78 773.1 0 78 65 60 773.1 0 60 66 82 773.1 0 82 67 62 773.1 0 62 68 75 773.1 0 75 69 90 773.1 0 90 70 132 773.1 0 132 71 298 773.1 0 298 72 83 773.1 0 83 73 71 773.1 0 71 74 48 773.1 0 48 75 57 773.1 0 57 76 68 773.1 0 68 77 71 773.1 0 71 78 82 773.1 0 82 79 234 773.1 0 234 80 106 773.1 0 106 81 249 773.1 0 249 82 186 773.1 0 186 83 1055 773.1 0 1055 84 1040 773.1 0 1040 85 1109 773.1 0 1109 86 59 773.1 0 59 87 78 773.1 0 78 88 67 773.1 0 67 89 72 773.1 0 72 90 154 773.1 0 154 91 65 773.1 0 65 92 56 773.1 0 56 93 65 773.1 0 65 94 65 773.1 0 65 95 69 773.1 0 69 96 158 773.1 0 158 97 93 773.1 0 93 98 262 773.1 0 262 99 168 773.1 0 168 100 164 773.1 0 164 101 124 773.1 0 124 102 71 773.1 0 71 103 121 773.1 0 121 104 69 773.1 0 69 105 226 773.1 0 226 106 115 773.1 0 115 107 140 773.1 0 140 108 112 773.1 0 112 109 121 773.1 0 121 110 174 773.1 0 174 111 186 773.1 0 186 112 285 773.1 0 285 113 4821 773.1 0 4821 114 2787 773.1 0 2787 115 5929 773.1 0 5929 116 207 773.1 0 207 117 73 773.1 0 73 118 98 773.1 0 98 119 130 773.1 0 130 120 90 773.1 0 90 121 74 773.1 0 74 122 119 773.1 0 119 123 99 773.1 0 99 124 122 773.1 0 122 125 151 773.1 0 151 126 126 773.1 0 126 127 124 773.1 0 124 128 159 773.1 0 159 129 2047 773.1 0 2047 130 2586 773.1 0 2586 131 3739 773.1 0 3739 132 98 773.1 0 98 133 73 773.1 0 73 134 93 773.1 0 93 135 91 773.1 0 91 136 102 773.1 0 102 137 95 773.1 0 95 138 130 773.1 0 130 139 124 773.1 0 124 140 118 773.1 0 118 141 133 773.1 0 133 142 97 773.1 0 97 143 96 773.1 0 96 144 85 773.1 0 85 145 91 773.1 0 91 146 94 773.1 0 94 147 102 773.1 0 102 148 101 773.1 0 101 149 192 773.1 0 192 150 121 773.1 0 121 151 108 773.1 0 108 152 111 773.1 0 111 153 167 773.1 0 167 154 1242 773.1 0 1242 155 1621 773.1 0 1621 156 3881 773.1 0 3881 157 2541 773.1 0 2541 158 941 773.1 0 941 159 185 773.1 0 185 160 60 773.1 0 60 161 49 773.1 0 49 162 51 773.1 0 51 163 95 773.1 0 95 164 124 773.1 0 124 165 71 773.1 0 71 166 59 773.1 0 59 167 154 773.1 0 154 168 148 773.1 0 148 169 164 773.1 0 164 170 131 773.1 0 131 171 90 773.1 0 90 172 88 773.1 0 88 173 70 773.1 0 70 174 76 773.1 0 76 175 71 773.1 0 71 176 68 773.1 0 68 177 65 773.1 0 65 178 423 773.1 0 423 179 59 773.1 0 59 180 82 773.1 0 82 181 46 773.1 0 46 182 59 773.1 0 59 183 61 773.1 0 61 184 81 773.1 0 81 185 88 773.1 0 88 186 86 773.1 0 86 187 67 773.1 0 67 188 137 773.1 0 137 189 78 773.1 0 78 190 107 773.1 0 107 191 73 773.1 0 73 192 78 773.1 0 78 193 72 773.1 0 72 194 73 773.1 0 73 195 61 773.1 0 61 196 75 773.1 0 75 197 80 773.1 0 80 198 77 773.1 0 77 199 62 773.1 0 62 200 227 773.1 0 227 201 117 773.1 0 117 202 198 773.1 0 198 203 69 773.1 0 69 204 100 773.1 0 100 205 76 773.1 0 76 206 69 773.1 0 69 207 71 773.1 0 71 208 69 773.1 0 69 209 72 773.1 0 72 210 65 773.1 0 65 211 108 773.1 0 108 212 98 773.1 0 98 213 101 773.1 0 101 214 118 773.1 0 118 215 118 773.1 0 118 216 105 773.1 0 105 217 68 773.1 0 68 218 116 773.1 0 116 219 73 773.1 0 73 220 62 773.1 0 62 221 69 773.1 0 69 222 79 773.1 0 79 223 77 773.1 0 77 224 77 773.1 0 77 225 76 773.1 0 76 226 68 773.1 0 68 227 73 773.1 0 73 228 309 773.1 0 309 229 268 773.1 0 268 230 392 773.1 0 392 231 112 773.1 0 112 232 93 773.1 0 93 233 106 773.1 0 106 234 99 773.1 0 99 235 110 773.1 0 110 236 130 773.1 0 130 237 111 773.1 0 111 238 80 773.1 0 80 239 95 773.1 0 95 240 80 773.1 0 80 241 46 773.1 0 46 242 80 773.1 0 80 243 124 773.1 0 124 244 94 773.1 0 94 245 78 773.1 0 78 246 59 773.1 0 59 247 107 773.1 0 107 248 147 773.1 0 147 249 411 773.1 0 411 250 72 773.1 0 72 251 93 773.1 0 93 252 88 773.1 0 88 253 71 773.1 0 71 254 74 773.1 0 74 255 116 773.1 0 116 256 100 773.1 0 100 257 140 773.1 0 140 258 102 773.1 0 102 259 62 773.1 0 62 260 66 773.1 0 66 261 64 773.1 0 64 262 73 773.1 0 73 263 90 773.1 0 90 264 69 773.1 0 69 265 64 773.1 0 64 266 73 773.1 0 73 267 74 773.1 0 74 268 114 773.1 0 114 269 181 773.1 0 181 270 218 773.1 0 218 271 152 773.1 0 152 272 82 773.1 0 82 273 74 773.1 0 74 274 90 773.1 0 90 275 65 773.1 0 65 276 127 773.1 0 127 277 111 773.1 0 111 278 73 773.1 0 73 279 84 773.1 0 84 280 86 773.1 0 86 281 133 773.1 0 133 282 138 773.1 0 138 283 135 773.1 0 135 284 140 773.1 0 140 285 302 773.1 0 302 286 139 773.1 0 139 287 119 773.1 0 119 288 160 773.1 0 160 289 125 773.1 0 125 290 126 773.1 0 126 291 164 773.1 0 164 292 132 773.1 0 132 293 138 773.1 0 138 294 459 773.1 0 459 295 973 773.1 0 973 296 2559 773.1 0 2559 297 1728 773.1 0 1728 298 4651 773.1 0 4651 299 7383 773.1 0 7383 300 7678 773.1 0 7678 301 210 773.1 0 210 Finished in 6.87 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 112,986 Reads with adapters: 1,710 (1.5%) Reads that were too short: 79 (0.1%) Reads written (passing filters): 1,631 (1.4%) Total basepairs processed: 26,926,971 bp Total written (filtered): 430,959 bp (1.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1710 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 10 0.0 3 0 10 17 1471 0.0 3 1438 33 18 4 0.0 3 0 4 19 6 0.0 3 5 0 1 20 1 0.0 3 1 23 5 0.0 3 0 0 0 5 32 83 0.0 3 0 0 1 82 34 2 0.0 3 0 0 0 2 35 6 0.0 3 0 0 0 6 38 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 6 0.0 3 0 0 0 6 53 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 58 7 0.0 3 0 0 0 7 59 2 0.0 3 0 0 0 2 62 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 10 0.0 3 0 0 9 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 1 88 2 0.0 3 1 0 0 1 94 1 0.0 3 1 104 1 0.0 3 0 0 1 109 3 0.0 3 0 0 0 3 111 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 1 1 119 1 0.0 3 1 122 2 0.0 3 0 0 0 2 123 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 4 0.0 3 0 0 0 4 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 196 1 0.0 3 1 197 1 0.0 3 1 200 24 0.0 3 0 0 0 24 204 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 236 4 0.0 3 0 0 0 4 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 1,631 Reads with adapters: 1 (0.1%) Reads written (passing filters): 1,631 (100.0%) Total basepairs processed: 430,959 bp Total written (filtered): 430,956 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 25.5 0 1