This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 143.86 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 146,991 (4.6%) Reads that were too short: 14,037 (0.4%) Reads written (passing filters): 132,954 (4.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 36,115,748 bp (3.8%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 146991 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 852 773.1 0 852 7 47555 773.1 0 47555 8 1749 773.1 0 1749 9 46887 773.1 0 46887 10 180 773.1 0 180 11 149 773.1 0 149 12 129 773.1 0 129 13 142 773.1 0 142 14 118 773.1 0 118 15 122 773.1 0 122 16 138 773.1 0 138 17 149 773.1 0 149 18 133 773.1 0 133 19 143 773.1 0 143 20 142 773.1 0 142 21 172 773.1 0 172 22 208 773.1 0 208 23 137 773.1 0 137 24 131 773.1 0 131 25 169 773.1 0 169 26 171 773.1 0 171 27 132 773.1 0 132 28 167 773.1 0 167 29 129 773.1 0 129 30 140 773.1 0 140 31 167 773.1 0 167 32 163 773.1 0 163 33 226 773.1 0 226 34 542 773.1 0 542 35 4626 773.1 0 4626 36 1634 773.1 0 1634 37 2346 773.1 0 2346 38 113 773.1 0 113 39 120 773.1 0 120 40 100 773.1 0 100 41 105 773.1 0 105 42 130 773.1 0 130 43 134 773.1 0 134 44 143 773.1 0 143 45 138 773.1 0 138 46 137 773.1 0 137 47 119 773.1 0 119 48 114 773.1 0 114 49 100 773.1 0 100 50 194 773.1 0 194 51 109 773.1 0 109 52 136 773.1 0 136 53 109 773.1 0 109 54 123 773.1 0 123 55 117 773.1 0 117 56 130 773.1 0 130 57 108 773.1 0 108 58 107 773.1 0 107 59 104 773.1 0 104 60 146 773.1 0 146 61 99 773.1 0 99 62 100 773.1 0 100 63 117 773.1 0 117 64 136 773.1 0 136 65 115 773.1 0 115 66 108 773.1 0 108 67 110 773.1 0 110 68 220 773.1 0 220 69 159 773.1 0 159 70 236 773.1 0 236 71 253 773.1 0 253 72 134 773.1 0 134 73 127 773.1 0 127 74 115 773.1 0 115 75 114 773.1 0 114 76 133 773.1 0 133 77 117 773.1 0 117 78 143 773.1 0 143 79 137 773.1 0 137 80 136 773.1 0 136 81 117 773.1 0 117 82 124 773.1 0 124 83 105 773.1 0 105 84 146 773.1 0 146 85 137 773.1 0 137 86 99 773.1 0 99 87 117 773.1 0 117 88 115 773.1 0 115 89 136 773.1 0 136 90 155 773.1 0 155 91 102 773.1 0 102 92 136 773.1 0 136 93 109 773.1 0 109 94 166 773.1 0 166 95 133 773.1 0 133 96 181 773.1 0 181 97 156 773.1 0 156 98 141 773.1 0 141 99 284 773.1 0 284 100 200 773.1 0 200 101 387 773.1 0 387 102 131 773.1 0 131 103 165 773.1 0 165 104 152 773.1 0 152 105 128 773.1 0 128 106 116 773.1 0 116 107 135 773.1 0 135 108 148 773.1 0 148 109 136 773.1 0 136 110 206 773.1 0 206 111 505 773.1 0 505 112 418 773.1 0 418 113 253 773.1 0 253 114 126 773.1 0 126 115 114 773.1 0 114 116 139 773.1 0 139 117 119 773.1 0 119 118 112 773.1 0 112 119 152 773.1 0 152 120 130 773.1 0 130 121 184 773.1 0 184 122 243 773.1 0 243 123 161 773.1 0 161 124 183 773.1 0 183 125 132 773.1 0 132 126 139 773.1 0 139 127 191 773.1 0 191 128 197 773.1 0 197 129 134 773.1 0 134 130 91 773.1 0 91 131 138 773.1 0 138 132 154 773.1 0 154 133 166 773.1 0 166 134 132 773.1 0 132 135 113 773.1 0 113 136 124 773.1 0 124 137 108 773.1 0 108 138 127 773.1 0 127 139 219 773.1 0 219 140 185 773.1 0 185 141 277 773.1 0 277 142 168 773.1 0 168 143 122 773.1 0 122 144 110 773.1 0 110 145 115 773.1 0 115 146 117 773.1 0 117 147 202 773.1 0 202 148 205 773.1 0 205 149 163 773.1 0 163 150 91 773.1 0 91 151 94 773.1 0 94 152 109 773.1 0 109 153 110 773.1 0 110 154 112 773.1 0 112 155 130 773.1 0 130 156 100 773.1 0 100 157 115 773.1 0 115 158 120 773.1 0 120 159 304 773.1 0 304 160 213 773.1 0 213 161 278 773.1 0 278 162 88 773.1 0 88 163 80 773.1 0 80 164 110 773.1 0 110 165 101 773.1 0 101 166 80 773.1 0 80 167 101 773.1 0 101 168 122 773.1 0 122 169 126 773.1 0 126 170 106 773.1 0 106 171 87 773.1 0 87 172 117 773.1 0 117 173 150 773.1 0 150 174 114 773.1 0 114 175 105 773.1 0 105 176 92 773.1 0 92 177 99 773.1 0 99 178 96 773.1 0 96 179 122 773.1 0 122 180 117 773.1 0 117 181 128 773.1 0 128 182 124 773.1 0 124 183 120 773.1 0 120 184 115 773.1 0 115 185 113 773.1 0 113 186 118 773.1 0 118 187 139 773.1 0 139 188 117 773.1 0 117 189 130 773.1 0 130 190 105 773.1 0 105 191 136 773.1 0 136 192 94 773.1 0 94 193 115 773.1 0 115 194 130 773.1 0 130 195 101 773.1 0 101 196 101 773.1 0 101 197 108 773.1 0 108 198 124 773.1 0 124 199 99 773.1 0 99 200 94 773.1 0 94 201 130 773.1 0 130 202 127 773.1 0 127 203 131 773.1 0 131 204 126 773.1 0 126 205 288 773.1 0 288 206 358 773.1 0 358 207 267 773.1 0 267 208 216 773.1 0 216 209 178 773.1 0 178 210 148 773.1 0 148 211 129 773.1 0 129 212 113 773.1 0 113 213 148 773.1 0 148 214 131 773.1 0 131 215 118 773.1 0 118 216 91 773.1 0 91 217 90 773.1 0 90 218 105 773.1 0 105 219 102 773.1 0 102 220 117 773.1 0 117 221 127 773.1 0 127 222 180 773.1 0 180 223 128 773.1 0 128 224 115 773.1 0 115 225 121 773.1 0 121 226 99 773.1 0 99 227 93 773.1 0 93 228 107 773.1 0 107 229 130 773.1 0 130 230 119 773.1 0 119 231 145 773.1 0 145 232 146 773.1 0 146 233 104 773.1 0 104 234 133 773.1 0 133 235 270 773.1 0 270 236 251 773.1 0 251 237 314 773.1 0 314 238 111 773.1 0 111 239 97 773.1 0 97 240 118 773.1 0 118 241 161 773.1 0 161 242 225 773.1 0 225 243 185 773.1 0 185 244 93 773.1 0 93 245 85 773.1 0 85 246 97 773.1 0 97 247 94 773.1 0 94 248 205 773.1 0 205 249 245 773.1 0 245 250 157 773.1 0 157 251 76 773.1 0 76 252 108 773.1 0 108 253 82 773.1 0 82 254 93 773.1 0 93 255 171 773.1 0 171 256 233 773.1 0 233 257 219 773.1 0 219 258 230 773.1 0 230 259 296 773.1 0 296 260 437 773.1 0 437 261 307 773.1 0 307 262 125 773.1 0 125 263 77 773.1 0 77 264 94 773.1 0 94 265 82 773.1 0 82 266 83 773.1 0 83 267 95 773.1 0 95 268 70 773.1 0 70 269 74 773.1 0 74 270 83 773.1 0 83 271 78 773.1 0 78 272 98 773.1 0 98 273 116 773.1 0 116 274 148 773.1 0 148 275 126 773.1 0 126 276 78 773.1 0 78 277 92 773.1 0 92 278 111 773.1 0 111 279 124 773.1 0 124 280 150 773.1 0 150 281 122 773.1 0 122 282 166 773.1 0 166 283 110 773.1 0 110 284 127 773.1 0 127 285 106 773.1 0 106 286 109 773.1 0 109 287 99 773.1 0 99 288 97 773.1 0 97 289 143 773.1 0 143 290 117 773.1 0 117 291 111 773.1 0 111 292 102 773.1 0 102 293 134 773.1 0 134 294 120 773.1 0 120 295 108 773.1 0 108 296 108 773.1 0 108 297 123 773.1 0 123 298 98 773.1 0 98 299 109 773.1 0 109 300 114 773.1 0 114 301 120 773.1 0 120 Finished in 9.96 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 132,954 Reads with adapters: 50,548 (38.0%) Reads that were too short: 45 (0.0%) Reads written (passing filters): 50,503 (38.0%) Total basepairs processed: 36,115,748 bp Total written (filtered): 13,509,784 bp (37.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 50548 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 14 0.0 3 0 0 10 2 2 19 550 0.0 3 0 462 63 14 11 20 46702 0.0 4 44695 1642 276 62 27 21 153 0.0 4 32 100 15 2 4 22 6 0.0 4 1 0 5 23 60 0.0 4 0 0 0 43 17 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 1 26 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 50 1 0.0 4 0 0 0 0 1 52 1 0.0 4 1 64 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 98 1 0.0 4 1 99 29 0.0 4 0 0 0 0 29 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 10 0.0 4 0 0 0 0 10 126 47 0.0 4 0 0 0 0 47 127 2899 0.0 4 0 0 0 0 2899 128 5 0.0 4 0 0 0 0 5 130 5 0.0 4 0 0 0 0 5 132 1 0.0 4 0 0 0 0 1 134 2 0.0 4 1 1 136 2 0.0 4 1 0 0 0 1 137 3 0.0 4 0 0 0 0 3 144 2 0.0 4 1 0 0 0 1 153 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 210 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 11 0.0 4 0 0 0 1 10 229 4 0.0 4 0 0 0 0 4 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 1 254 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.79 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 50,503 Reads with adapters: 1,920 (3.8%) Reads written (passing filters): 50,503 (100.0%) Total basepairs processed: 13,509,784 bp Total written (filtered): 13,475,322 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1920 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.6% C: 11.2% G: 73.0% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 232 789.1 0 232 4 16 197.3 0 16 5 1 49.3 0 1 7 1 3.1 0 1 9 6 0.2 0 0 6 10 14 0.0 1 2 12 11 701 0.0 1 15 686 12 863 0.0 1 22 841 13 7 0.0 1 0 7 15 3 0.0 1 0 3 152 6 0.0 1 4 2 153 1 0.0 1 1 179 12 0.0 1 8 4 208 1 0.0 1 1 210 47 0.0 1 41 6 211 2 0.0 1 2 225 6 0.0 1 5 1 230 1 0.0 1 1