This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 141.32 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 141,291 (4.5%) Reads that were too short: 12,026 (0.4%) Reads written (passing filters): 129,265 (4.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 35,458,691 bp (3.7%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 141291 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 735 773.1 0 735 7 52937 773.1 0 52937 8 1435 773.1 0 1435 9 44527 773.1 0 44527 10 155 773.1 0 155 11 108 773.1 0 108 12 107 773.1 0 107 13 126 773.1 0 126 14 96 773.1 0 96 15 103 773.1 0 103 16 103 773.1 0 103 17 119 773.1 0 119 18 129 773.1 0 129 19 114 773.1 0 114 20 119 773.1 0 119 21 143 773.1 0 143 22 163 773.1 0 163 23 125 773.1 0 125 24 130 773.1 0 130 25 155 773.1 0 155 26 153 773.1 0 153 27 112 773.1 0 112 28 111 773.1 0 111 29 124 773.1 0 124 30 133 773.1 0 133 31 113 773.1 0 113 32 120 773.1 0 120 33 148 773.1 0 148 34 218 773.1 0 218 35 3490 773.1 0 3490 36 1260 773.1 0 1260 37 2042 773.1 0 2042 38 93 773.1 0 93 39 78 773.1 0 78 40 81 773.1 0 81 41 76 773.1 0 76 42 98 773.1 0 98 43 109 773.1 0 109 44 86 773.1 0 86 45 89 773.1 0 89 46 105 773.1 0 105 47 99 773.1 0 99 48 80 773.1 0 80 49 85 773.1 0 85 50 160 773.1 0 160 51 83 773.1 0 83 52 106 773.1 0 106 53 88 773.1 0 88 54 100 773.1 0 100 55 91 773.1 0 91 56 90 773.1 0 90 57 82 773.1 0 82 58 109 773.1 0 109 59 86 773.1 0 86 60 147 773.1 0 147 61 114 773.1 0 114 62 99 773.1 0 99 63 115 773.1 0 115 64 108 773.1 0 108 65 116 773.1 0 116 66 98 773.1 0 98 67 93 773.1 0 93 68 185 773.1 0 185 69 150 773.1 0 150 70 168 773.1 0 168 71 222 773.1 0 222 72 98 773.1 0 98 73 86 773.1 0 86 74 74 773.1 0 74 75 85 773.1 0 85 76 100 773.1 0 100 77 99 773.1 0 99 78 81 773.1 0 81 79 124 773.1 0 124 80 111 773.1 0 111 81 97 773.1 0 97 82 105 773.1 0 105 83 106 773.1 0 106 84 110 773.1 0 110 85 132 773.1 0 132 86 104 773.1 0 104 87 118 773.1 0 118 88 122 773.1 0 122 89 115 773.1 0 115 90 144 773.1 0 144 91 110 773.1 0 110 92 97 773.1 0 97 93 95 773.1 0 95 94 99 773.1 0 99 95 113 773.1 0 113 96 129 773.1 0 129 97 126 773.1 0 126 98 123 773.1 0 123 99 312 773.1 0 312 100 175 773.1 0 175 101 382 773.1 0 382 102 131 773.1 0 131 103 138 773.1 0 138 104 119 773.1 0 119 105 111 773.1 0 111 106 106 773.1 0 106 107 113 773.1 0 113 108 114 773.1 0 114 109 105 773.1 0 105 110 153 773.1 0 153 111 376 773.1 0 376 112 310 773.1 0 310 113 259 773.1 0 259 114 125 773.1 0 125 115 91 773.1 0 91 116 95 773.1 0 95 117 99 773.1 0 99 118 90 773.1 0 90 119 102 773.1 0 102 120 88 773.1 0 88 121 162 773.1 0 162 122 188 773.1 0 188 123 107 773.1 0 107 124 131 773.1 0 131 125 81 773.1 0 81 126 121 773.1 0 121 127 122 773.1 0 122 128 141 773.1 0 141 129 131 773.1 0 131 130 121 773.1 0 121 131 126 773.1 0 126 132 133 773.1 0 133 133 129 773.1 0 129 134 100 773.1 0 100 135 87 773.1 0 87 136 109 773.1 0 109 137 81 773.1 0 81 138 107 773.1 0 107 139 170 773.1 0 170 140 131 773.1 0 131 141 198 773.1 0 198 142 130 773.1 0 130 143 96 773.1 0 96 144 96 773.1 0 96 145 110 773.1 0 110 146 95 773.1 0 95 147 137 773.1 0 137 148 118 773.1 0 118 149 130 773.1 0 130 150 97 773.1 0 97 151 81 773.1 0 81 152 111 773.1 0 111 153 92 773.1 0 92 154 64 773.1 0 64 155 100 773.1 0 100 156 89 773.1 0 89 157 106 773.1 0 106 158 113 773.1 0 113 159 371 773.1 0 371 160 204 773.1 0 204 161 325 773.1 0 325 162 72 773.1 0 72 163 68 773.1 0 68 164 107 773.1 0 107 165 93 773.1 0 93 166 76 773.1 0 76 167 111 773.1 0 111 168 114 773.1 0 114 169 103 773.1 0 103 170 100 773.1 0 100 171 78 773.1 0 78 172 87 773.1 0 87 173 159 773.1 0 159 174 87 773.1 0 87 175 106 773.1 0 106 176 91 773.1 0 91 177 102 773.1 0 102 178 106 773.1 0 106 179 103 773.1 0 103 180 105 773.1 0 105 181 108 773.1 0 108 182 105 773.1 0 105 183 114 773.1 0 114 184 123 773.1 0 123 185 126 773.1 0 126 186 106 773.1 0 106 187 109 773.1 0 109 188 105 773.1 0 105 189 112 773.1 0 112 190 102 773.1 0 102 191 94 773.1 0 94 192 92 773.1 0 92 193 89 773.1 0 89 194 123 773.1 0 123 195 102 773.1 0 102 196 103 773.1 0 103 197 107 773.1 0 107 198 92 773.1 0 92 199 106 773.1 0 106 200 93 773.1 0 93 201 106 773.1 0 106 202 118 773.1 0 118 203 104 773.1 0 104 204 117 773.1 0 117 205 244 773.1 0 244 206 311 773.1 0 311 207 282 773.1 0 282 208 174 773.1 0 174 209 151 773.1 0 151 210 96 773.1 0 96 211 102 773.1 0 102 212 87 773.1 0 87 213 106 773.1 0 106 214 107 773.1 0 107 215 74 773.1 0 74 216 95 773.1 0 95 217 83 773.1 0 83 218 105 773.1 0 105 219 83 773.1 0 83 220 103 773.1 0 103 221 101 773.1 0 101 222 111 773.1 0 111 223 93 773.1 0 93 224 91 773.1 0 91 225 114 773.1 0 114 226 76 773.1 0 76 227 77 773.1 0 77 228 87 773.1 0 87 229 109 773.1 0 109 230 90 773.1 0 90 231 113 773.1 0 113 232 122 773.1 0 122 233 104 773.1 0 104 234 106 773.1 0 106 235 254 773.1 0 254 236 257 773.1 0 257 237 308 773.1 0 308 238 104 773.1 0 104 239 97 773.1 0 97 240 96 773.1 0 96 241 176 773.1 0 176 242 220 773.1 0 220 243 185 773.1 0 185 244 106 773.1 0 106 245 65 773.1 0 65 246 76 773.1 0 76 247 68 773.1 0 68 248 197 773.1 0 197 249 231 773.1 0 231 250 164 773.1 0 164 251 82 773.1 0 82 252 82 773.1 0 82 253 73 773.1 0 73 254 88 773.1 0 88 255 127 773.1 0 127 256 188 773.1 0 188 257 149 773.1 0 149 258 145 773.1 0 145 259 267 773.1 0 267 260 300 773.1 0 300 261 183 773.1 0 183 262 114 773.1 0 114 263 86 773.1 0 86 264 74 773.1 0 74 265 95 773.1 0 95 266 54 773.1 0 54 267 78 773.1 0 78 268 77 773.1 0 77 269 85 773.1 0 85 270 73 773.1 0 73 271 82 773.1 0 82 272 76 773.1 0 76 273 104 773.1 0 104 274 117 773.1 0 117 275 117 773.1 0 117 276 65 773.1 0 65 277 75 773.1 0 75 278 102 773.1 0 102 279 83 773.1 0 83 280 104 773.1 0 104 281 103 773.1 0 103 282 136 773.1 0 136 283 124 773.1 0 124 284 111 773.1 0 111 285 94 773.1 0 94 286 88 773.1 0 88 287 114 773.1 0 114 288 90 773.1 0 90 289 99 773.1 0 99 290 126 773.1 0 126 291 125 773.1 0 125 292 90 773.1 0 90 293 86 773.1 0 86 294 110 773.1 0 110 295 89 773.1 0 89 296 99 773.1 0 99 297 90 773.1 0 90 298 85 773.1 0 85 299 94 773.1 0 94 300 96 773.1 0 96 301 92 773.1 0 92 Finished in 8.75 s (68 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 129,265 Reads with adapters: 46,325 (35.8%) Reads that were too short: 175 (0.1%) Reads written (passing filters): 46,150 (35.7%) Total basepairs processed: 35,458,691 bp Total written (filtered): 12,679,486 bp (35.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 46325 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 9 16 272 0.0 3 0 268 4 17 45603 0.0 3 44608 967 24 4 18 106 0.0 3 17 85 3 1 19 33 0.0 3 0 0 31 2 20 29 0.0 3 1 0 0 28 21 1 0.0 3 0 1 22 2 0.0 3 0 0 0 2 27 1 0.0 3 1 29 1 0.0 3 1 31 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 70 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 81 7 0.0 3 0 0 0 7 87 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 131 10 0.0 3 0 1 0 9 132 10 0.0 3 0 0 0 10 134 2 0.0 3 0 0 0 2 136 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 146 19 0.0 3 0 0 0 19 147 5 0.0 3 0 0 0 5 148 12 0.0 3 0 0 0 12 149 3 0.0 3 0 0 0 3 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 169 27 0.0 3 0 0 0 27 178 1 0.0 3 0 0 0 1 193 1 0.0 3 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 131 0.0 3 0 0 3 128 218 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 1 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 1 1 250 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.46 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 46,150 Reads with adapters: 596 (1.3%) Reads written (passing filters): 46,150 (100.0%) Total basepairs processed: 12,679,486 bp Total written (filtered): 12,677,641 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 596 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.2% C: 4.9% G: 17.4% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 541 721.1 0 541 4 53 180.3 0 53 5 2 45.1 0 2