This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 147.32 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 352,733 (11.1%) Reads that were too short: 11,069 (0.3%) Reads written (passing filters): 341,664 (10.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 78,483,603 bp (8.2%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 352733 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 786 773.1 0 786 7 1975 773.1 0 1975 8 52243 773.1 0 52243 9 45491 773.1 0 45491 10 103 773.1 0 103 11 52 773.1 0 52 12 64 773.1 0 64 13 43 773.1 0 43 14 39 773.1 0 39 15 32 773.1 0 32 16 37 773.1 0 37 17 30 773.1 0 30 18 34 773.1 0 34 19 35 773.1 0 35 20 38 773.1 0 38 21 49 773.1 0 49 22 35 773.1 0 35 23 41 773.1 0 41 24 107 773.1 0 107 25 82 773.1 0 82 26 73 773.1 0 73 27 42 773.1 0 42 28 49 773.1 0 49 29 29 773.1 0 29 30 62 773.1 0 62 31 112 773.1 0 112 32 53 773.1 0 53 33 46 773.1 0 46 34 107 773.1 0 107 35 88 773.1 0 88 36 88 773.1 0 88 37 410 773.1 0 410 38 82 773.1 0 82 39 56 773.1 0 56 40 76 773.1 0 76 41 52 773.1 0 52 42 55 773.1 0 55 43 59 773.1 0 59 44 51 773.1 0 51 45 52 773.1 0 52 46 85 773.1 0 85 47 114 773.1 0 114 48 49 773.1 0 49 49 52 773.1 0 52 50 44 773.1 0 44 51 86 773.1 0 86 52 60 773.1 0 60 53 139 773.1 0 139 54 257 773.1 0 257 55 334 773.1 0 334 56 70 773.1 0 70 57 66 773.1 0 66 58 68 773.1 0 68 59 29 773.1 0 29 60 47 773.1 0 47 61 70 773.1 0 70 62 68 773.1 0 68 63 110 773.1 0 110 64 213 773.1 0 213 65 89 773.1 0 89 66 158 773.1 0 158 67 125 773.1 0 125 68 125 773.1 0 125 69 304 773.1 0 304 70 123 773.1 0 123 71 116 773.1 0 116 72 88 773.1 0 88 73 79 773.1 0 79 74 140 773.1 0 140 75 174 773.1 0 174 76 330 773.1 0 330 77 407 773.1 0 407 78 637 773.1 0 637 79 1533 773.1 0 1533 80 3996 773.1 0 3996 81 63065 773.1 0 63065 82 43255 773.1 0 43255 83 41612 773.1 0 41612 84 228 773.1 0 228 85 319 773.1 0 319 86 318 773.1 0 318 87 589 773.1 0 589 88 60 773.1 0 60 89 44 773.1 0 44 90 39 773.1 0 39 91 34 773.1 0 34 92 41 773.1 0 41 93 34 773.1 0 34 94 48 773.1 0 48 95 55 773.1 0 55 96 55 773.1 0 55 97 50 773.1 0 50 98 83 773.1 0 83 99 57 773.1 0 57 100 86 773.1 0 86 101 62 773.1 0 62 102 143 773.1 0 143 103 76 773.1 0 76 104 174 773.1 0 174 105 73 773.1 0 73 106 87 773.1 0 87 107 92 773.1 0 92 108 175 773.1 0 175 109 276 773.1 0 276 110 97 773.1 0 97 111 219 773.1 0 219 112 168 773.1 0 168 113 90 773.1 0 90 114 115 773.1 0 115 115 162 773.1 0 162 116 140 773.1 0 140 117 202 773.1 0 202 118 213 773.1 0 213 119 778 773.1 0 778 120 696 773.1 0 696 121 1120 773.1 0 1120 122 21223 773.1 0 21223 123 12723 773.1 0 12723 124 21545 773.1 0 21545 125 194 773.1 0 194 126 173 773.1 0 173 127 83 773.1 0 83 128 59 773.1 0 59 129 67 773.1 0 67 130 38 773.1 0 38 131 54 773.1 0 54 132 44 773.1 0 44 133 54 773.1 0 54 134 62 773.1 0 62 135 56 773.1 0 56 136 52 773.1 0 52 137 58 773.1 0 58 138 62 773.1 0 62 139 81 773.1 0 81 140 56 773.1 0 56 141 80 773.1 0 80 142 534 773.1 0 534 143 415 773.1 0 415 144 1029 773.1 0 1029 145 577 773.1 0 577 146 744 773.1 0 744 147 304 773.1 0 304 148 115 773.1 0 115 149 245 773.1 0 245 150 136 773.1 0 136 151 461 773.1 0 461 152 95 773.1 0 95 153 202 773.1 0 202 154 86 773.1 0 86 155 76 773.1 0 76 156 46 773.1 0 46 157 25 773.1 0 25 158 40 773.1 0 40 159 40 773.1 0 40 160 63 773.1 0 63 161 58 773.1 0 58 162 45 773.1 0 45 163 50 773.1 0 50 164 58 773.1 0 58 165 38 773.1 0 38 166 78 773.1 0 78 167 302 773.1 0 302 168 54 773.1 0 54 169 169 773.1 0 169 170 125 773.1 0 125 171 89 773.1 0 89 172 39 773.1 0 39 173 57 773.1 0 57 174 59 773.1 0 59 175 76 773.1 0 76 176 47 773.1 0 47 177 51 773.1 0 51 178 47 773.1 0 47 179 60 773.1 0 60 180 113 773.1 0 113 181 223 773.1 0 223 182 3079 773.1 0 3079 183 1970 773.1 0 1970 184 3040 773.1 0 3040 185 93 773.1 0 93 186 68 773.1 0 68 187 78 773.1 0 78 188 44 773.1 0 44 189 39 773.1 0 39 190 52 773.1 0 52 191 38 773.1 0 38 192 32 773.1 0 32 193 77 773.1 0 77 194 129 773.1 0 129 195 107 773.1 0 107 196 63 773.1 0 63 197 35 773.1 0 35 198 91 773.1 0 91 199 69 773.1 0 69 200 97 773.1 0 97 201 55 773.1 0 55 202 30 773.1 0 30 203 33 773.1 0 33 204 24 773.1 0 24 205 59 773.1 0 59 206 67 773.1 0 67 207 54 773.1 0 54 208 34 773.1 0 34 209 36 773.1 0 36 210 66 773.1 0 66 211 60 773.1 0 60 212 57 773.1 0 57 213 33 773.1 0 33 214 44 773.1 0 44 215 29 773.1 0 29 216 35 773.1 0 35 217 31 773.1 0 31 218 38 773.1 0 38 219 59 773.1 0 59 220 62 773.1 0 62 221 41 773.1 0 41 222 35 773.1 0 35 223 37 773.1 0 37 224 36 773.1 0 36 225 45 773.1 0 45 226 67 773.1 0 67 227 84 773.1 0 84 228 109 773.1 0 109 229 56 773.1 0 56 230 40 773.1 0 40 231 50 773.1 0 50 232 34 773.1 0 34 233 41 773.1 0 41 234 29 773.1 0 29 235 31 773.1 0 31 236 35 773.1 0 35 237 46 773.1 0 46 238 52 773.1 0 52 239 38 773.1 0 38 240 198 773.1 0 198 241 122 773.1 0 122 242 108 773.1 0 108 243 34 773.1 0 34 244 39 773.1 0 39 245 50 773.1 0 50 246 39 773.1 0 39 247 47 773.1 0 47 248 54 773.1 0 54 249 61 773.1 0 61 250 36 773.1 0 36 251 37 773.1 0 37 252 46 773.1 0 46 253 69 773.1 0 69 254 62 773.1 0 62 255 84 773.1 0 84 256 61 773.1 0 61 257 62 773.1 0 62 258 76 773.1 0 76 259 93 773.1 0 93 260 103 773.1 0 103 261 194 773.1 0 194 262 108 773.1 0 108 263 182 773.1 0 182 264 135 773.1 0 135 265 90 773.1 0 90 266 768 773.1 0 768 267 634 773.1 0 634 268 904 773.1 0 904 269 160 773.1 0 160 270 76 773.1 0 76 271 46 773.1 0 46 272 57 773.1 0 57 273 66 773.1 0 66 274 264 773.1 0 264 275 282 773.1 0 282 276 374 773.1 0 374 277 158 773.1 0 158 278 81 773.1 0 81 279 140 773.1 0 140 280 127 773.1 0 127 281 193 773.1 0 193 282 69 773.1 0 69 283 72 773.1 0 72 284 36 773.1 0 36 285 146 773.1 0 146 286 263 773.1 0 263 287 279 773.1 0 279 288 233 773.1 0 233 289 158 773.1 0 158 290 176 773.1 0 176 291 66 773.1 0 66 292 108 773.1 0 108 293 152 773.1 0 152 294 224 773.1 0 224 295 189 773.1 0 189 296 139 773.1 0 139 297 70 773.1 0 70 298 100 773.1 0 100 299 96 773.1 0 96 300 109 773.1 0 109 301 107 773.1 0 107 Finished in 22.16 s (65 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 341,664 Reads with adapters: 48,969 (14.3%) Reads that were too short: 79 (0.0%) Reads written (passing filters): 48,890 (14.3%) Total basepairs processed: 78,483,603 bp Total written (filtered): 13,119,385 bp (16.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 48969 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 0 2 18 33 0.0 3 0 0 26 3 4 19 789 0.0 3 0 697 50 34 8 20 46005 0.0 4 43688 1870 346 82 19 21 111 0.0 4 15 87 6 2 1 22 99 0.0 4 83 4 11 0 1 23 142 0.0 4 0 0 1 107 34 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 60 1 0.0 4 1 63 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 0 2 94 2 0.0 4 0 0 0 0 2 99 12 0.0 4 0 0 0 0 12 100 1 0.0 4 0 0 0 1 101 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 18 0.0 4 0 0 0 0 18 127 1659 0.0 4 0 0 0 0 1659 128 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 148 8 0.0 4 0 0 0 0 8 149 3 0.0 4 0 0 0 0 3 150 5 0.0 4 0 0 0 0 5 151 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 179 6 0.0 4 0 0 0 0 6 185 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 189 3 0.0 4 2 0 0 0 1 191 1 0.0 4 0 0 0 1 192 1 0.0 4 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 1 4 229 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 238 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 3 0.0 4 0 0 0 0 3 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.70 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 48,890 Reads with adapters: 11,075 (22.7%) Reads written (passing filters): 48,890 (100.0%) Total basepairs processed: 13,119,385 bp Total written (filtered): 13,008,699 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11075 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 5.8% G: 91.1% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 763.9 0 14 4 10 191.0 0 10 5 1 47.7 0 1 7 1 3.0 0 1 8 3 0.7 0 3 9 3744 0.2 0 223 3521 10 6081 0.0 1 382 5699 11 1154 0.0 1 36 1118 12 26 0.0 1 1 25 13 27 0.0 1 0 27 72 1 0.0 1 1 150 1 0.0 1 1 169 2 0.0 1 1 1 170 1 0.0 1 1 189 1 0.0 1 0 1 208 6 0.0 1 5 1 269 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.