This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 146.52 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 371,589 (11.7%) Reads that were too short: 9,697 (0.3%) Reads written (passing filters): 361,892 (11.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 83,081,956 bp (8.7%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 371589 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 609 773.1 0 609 7 1166 773.1 0 1166 8 48661 773.1 0 48661 9 53873 773.1 0 53873 10 81 773.1 0 81 11 65 773.1 0 65 12 34 773.1 0 34 13 55 773.1 0 55 14 41 773.1 0 41 15 40 773.1 0 40 16 43 773.1 0 43 17 39 773.1 0 39 18 56 773.1 0 56 19 49 773.1 0 49 20 35 773.1 0 35 21 45 773.1 0 45 22 50 773.1 0 50 23 33 773.1 0 33 24 54 773.1 0 54 25 40 773.1 0 40 26 44 773.1 0 44 27 49 773.1 0 49 28 66 773.1 0 66 29 30 773.1 0 30 30 73 773.1 0 73 31 98 773.1 0 98 32 60 773.1 0 60 33 60 773.1 0 60 34 75 773.1 0 75 35 72 773.1 0 72 36 66 773.1 0 66 37 462 773.1 0 462 38 84 773.1 0 84 39 84 773.1 0 84 40 70 773.1 0 70 41 55 773.1 0 55 42 74 773.1 0 74 43 48 773.1 0 48 44 60 773.1 0 60 45 53 773.1 0 53 46 92 773.1 0 92 47 140 773.1 0 140 48 55 773.1 0 55 49 71 773.1 0 71 50 53 773.1 0 53 51 94 773.1 0 94 52 80 773.1 0 80 53 127 773.1 0 127 54 228 773.1 0 228 55 178 773.1 0 178 56 66 773.1 0 66 57 71 773.1 0 71 58 101 773.1 0 101 59 39 773.1 0 39 60 65 773.1 0 65 61 98 773.1 0 98 62 73 773.1 0 73 63 126 773.1 0 126 64 268 773.1 0 268 65 105 773.1 0 105 66 168 773.1 0 168 67 134 773.1 0 134 68 115 773.1 0 115 69 347 773.1 0 347 70 129 773.1 0 129 71 81 773.1 0 81 72 94 773.1 0 94 73 100 773.1 0 100 74 145 773.1 0 145 75 203 773.1 0 203 76 304 773.1 0 304 77 366 773.1 0 366 78 653 773.1 0 653 79 1488 773.1 0 1488 80 3448 773.1 0 3448 81 71104 773.1 0 71104 82 47476 773.1 0 47476 83 45762 773.1 0 45762 84 244 773.1 0 244 85 249 773.1 0 249 86 120 773.1 0 120 87 305 773.1 0 305 88 69 773.1 0 69 89 51 773.1 0 51 90 56 773.1 0 56 91 44 773.1 0 44 92 38 773.1 0 38 93 44 773.1 0 44 94 47 773.1 0 47 95 64 773.1 0 64 96 78 773.1 0 78 97 51 773.1 0 51 98 81 773.1 0 81 99 73 773.1 0 73 100 76 773.1 0 76 101 85 773.1 0 85 102 128 773.1 0 128 103 102 773.1 0 102 104 130 773.1 0 130 105 80 773.1 0 80 106 141 773.1 0 141 107 134 773.1 0 134 108 193 773.1 0 193 109 337 773.1 0 337 110 88 773.1 0 88 111 247 773.1 0 247 112 205 773.1 0 205 113 103 773.1 0 103 114 122 773.1 0 122 115 209 773.1 0 209 116 189 773.1 0 189 117 202 773.1 0 202 118 186 773.1 0 186 119 491 773.1 0 491 120 515 773.1 0 515 121 730 773.1 0 730 122 21801 773.1 0 21801 123 12455 773.1 0 12455 124 21899 773.1 0 21899 125 147 773.1 0 147 126 100 773.1 0 100 127 75 773.1 0 75 128 68 773.1 0 68 129 75 773.1 0 75 130 66 773.1 0 66 131 68 773.1 0 68 132 49 773.1 0 49 133 53 773.1 0 53 134 43 773.1 0 43 135 58 773.1 0 58 136 60 773.1 0 60 137 73 773.1 0 73 138 58 773.1 0 58 139 73 773.1 0 73 140 65 773.1 0 65 141 88 773.1 0 88 142 589 773.1 0 589 143 397 773.1 0 397 144 1014 773.1 0 1014 145 504 773.1 0 504 146 579 773.1 0 579 147 293 773.1 0 293 148 73 773.1 0 73 149 132 773.1 0 132 150 102 773.1 0 102 151 300 773.1 0 300 152 107 773.1 0 107 153 182 773.1 0 182 154 62 773.1 0 62 155 62 773.1 0 62 156 35 773.1 0 35 157 36 773.1 0 36 158 46 773.1 0 46 159 44 773.1 0 44 160 70 773.1 0 70 161 61 773.1 0 61 162 48 773.1 0 48 163 53 773.1 0 53 164 65 773.1 0 65 165 77 773.1 0 77 166 64 773.1 0 64 167 305 773.1 0 305 168 79 773.1 0 79 169 162 773.1 0 162 170 103 773.1 0 103 171 86 773.1 0 86 172 58 773.1 0 58 173 54 773.1 0 54 174 43 773.1 0 43 175 64 773.1 0 64 176 58 773.1 0 58 177 72 773.1 0 72 178 81 773.1 0 81 179 78 773.1 0 78 180 113 773.1 0 113 181 226 773.1 0 226 182 3639 773.1 0 3639 183 2252 773.1 0 2252 184 3723 773.1 0 3723 185 88 773.1 0 88 186 69 773.1 0 69 187 65 773.1 0 65 188 35 773.1 0 35 189 62 773.1 0 62 190 63 773.1 0 63 191 31 773.1 0 31 192 48 773.1 0 48 193 64 773.1 0 64 194 77 773.1 0 77 195 67 773.1 0 67 196 59 773.1 0 59 197 43 773.1 0 43 198 58 773.1 0 58 199 70 773.1 0 70 200 70 773.1 0 70 201 52 773.1 0 52 202 57 773.1 0 57 203 44 773.1 0 44 204 31 773.1 0 31 205 54 773.1 0 54 206 71 773.1 0 71 207 48 773.1 0 48 208 37 773.1 0 37 209 56 773.1 0 56 210 36 773.1 0 36 211 50 773.1 0 50 212 49 773.1 0 49 213 38 773.1 0 38 214 51 773.1 0 51 215 49 773.1 0 49 216 34 773.1 0 34 217 39 773.1 0 39 218 53 773.1 0 53 219 68 773.1 0 68 220 66 773.1 0 66 221 55 773.1 0 55 222 43 773.1 0 43 223 42 773.1 0 42 224 54 773.1 0 54 225 53 773.1 0 53 226 60 773.1 0 60 227 57 773.1 0 57 228 64 773.1 0 64 229 68 773.1 0 68 230 52 773.1 0 52 231 57 773.1 0 57 232 28 773.1 0 28 233 45 773.1 0 45 234 49 773.1 0 49 235 35 773.1 0 35 236 57 773.1 0 57 237 78 773.1 0 78 238 58 773.1 0 58 239 47 773.1 0 47 240 211 773.1 0 211 241 128 773.1 0 128 242 104 773.1 0 104 243 41 773.1 0 41 244 34 773.1 0 34 245 66 773.1 0 66 246 40 773.1 0 40 247 46 773.1 0 46 248 42 773.1 0 42 249 46 773.1 0 46 250 42 773.1 0 42 251 50 773.1 0 50 252 49 773.1 0 49 253 64 773.1 0 64 254 62 773.1 0 62 255 70 773.1 0 70 256 66 773.1 0 66 257 61 773.1 0 61 258 79 773.1 0 79 259 63 773.1 0 63 260 76 773.1 0 76 261 112 773.1 0 112 262 76 773.1 0 76 263 144 773.1 0 144 264 118 773.1 0 118 265 110 773.1 0 110 266 745 773.1 0 745 267 522 773.1 0 522 268 855 773.1 0 855 269 140 773.1 0 140 270 57 773.1 0 57 271 58 773.1 0 58 272 59 773.1 0 59 273 49 773.1 0 49 274 220 773.1 0 220 275 219 773.1 0 219 276 322 773.1 0 322 277 62 773.1 0 62 278 72 773.1 0 72 279 72 773.1 0 72 280 65 773.1 0 65 281 135 773.1 0 135 282 81 773.1 0 81 283 75 773.1 0 75 284 56 773.1 0 56 285 96 773.1 0 96 286 220 773.1 0 220 287 129 773.1 0 129 288 150 773.1 0 150 289 98 773.1 0 98 290 95 773.1 0 95 291 48 773.1 0 48 292 95 773.1 0 95 293 114 773.1 0 114 294 155 773.1 0 155 295 190 773.1 0 190 296 102 773.1 0 102 297 68 773.1 0 68 298 79 773.1 0 79 299 79 773.1 0 79 300 99 773.1 0 99 301 83 773.1 0 83 Finished in 21.62 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 361,892 Reads with adapters: 49,696 (13.7%) Reads that were too short: 200 (0.1%) Reads written (passing filters): 49,496 (13.7%) Total basepairs processed: 83,081,956 bp Total written (filtered): 13,542,914 bp (16.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49696 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 14 0.0 3 0 0 14 16 262 0.0 3 0 256 5 1 17 48265 0.0 3 47367 867 24 7 18 122 0.0 3 7 111 4 19 26 0.0 3 0 0 24 2 20 38 0.0 3 0 0 0 38 21 1 0.0 3 1 22 14 0.0 3 0 0 0 14 24 2 0.0 3 0 0 0 2 25 12 0.0 3 0 0 10 2 28 40 0.0 3 0 0 0 40 29 2 0.0 3 0 0 1 1 34 301 0.0 3 0 0 0 301 40 1 0.0 3 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 0 4 48 2 0.0 3 1 0 0 1 49 42 0.0 3 0 0 0 42 50 248 0.0 3 0 0 0 248 51 17 0.0 3 0 0 0 17 52 22 0.0 3 0 0 1 21 53 32 0.0 3 0 0 1 31 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 9 0.0 3 0 0 0 9 77 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 118 3 0.0 3 0 0 0 3 121 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 1 157 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 46 0.0 3 0 0 1 45 163 9 0.0 3 0 0 0 9 164 12 0.0 3 0 0 1 11 165 70 0.0 3 0 1 5 64 166 6 0.0 3 0 0 0 6 167 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 189 1 0.0 3 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.79 s (56 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 49,496 Reads with adapters: 232 (0.5%) Reads written (passing filters): 49,496 (100.0%) Total basepairs processed: 13,542,914 bp Total written (filtered): 13,542,147 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 232 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.2% C: 10.3% G: 6.0% T: 6.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 165 773.4 0 165 4 63 193.3 0 63 5 4 48.3 0 4