This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 143.19 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 719,354 (22.7%) Reads that were too short: 588,480 (18.6%) Reads written (passing filters): 130,874 (4.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 21,499,940 bp (2.3%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 719354 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2487 773.1 0 2487 7 12670 773.1 0 12670 8 537 773.1 0 537 9 15634 773.1 0 15634 10 93 773.1 0 93 11 63 773.1 0 63 12 58 773.1 0 58 13 50 773.1 0 50 14 36 773.1 0 36 15 41 773.1 0 41 16 27 773.1 0 27 17 50 773.1 0 50 18 36 773.1 0 36 19 35 773.1 0 35 20 32 773.1 0 32 21 46 773.1 0 46 22 46 773.1 0 46 23 28 773.1 0 28 24 39 773.1 0 39 25 31 773.1 0 31 26 29 773.1 0 29 27 10 773.1 0 10 28 29 773.1 0 29 29 40 773.1 0 40 30 45 773.1 0 45 31 214 773.1 0 214 32 142 773.1 0 142 33 71 773.1 0 71 34 97 773.1 0 97 35 108 773.1 0 108 36 44 773.1 0 44 37 58 773.1 0 58 38 50 773.1 0 50 39 57 773.1 0 57 40 34 773.1 0 34 41 57 773.1 0 57 42 40 773.1 0 40 43 30 773.1 0 30 44 34 773.1 0 34 45 47 773.1 0 47 46 33 773.1 0 33 47 43 773.1 0 43 48 36 773.1 0 36 49 30 773.1 0 30 50 37 773.1 0 37 51 22 773.1 0 22 52 34 773.1 0 34 53 34 773.1 0 34 54 50 773.1 0 50 55 39 773.1 0 39 56 58 773.1 0 58 57 81 773.1 0 81 58 65 773.1 0 65 59 73 773.1 0 73 60 115 773.1 0 115 61 46 773.1 0 46 62 31 773.1 0 31 63 32 773.1 0 32 64 39 773.1 0 39 65 80 773.1 0 80 66 93 773.1 0 93 67 56 773.1 0 56 68 25 773.1 0 25 69 35 773.1 0 35 70 23 773.1 0 23 71 47 773.1 0 47 72 35 773.1 0 35 73 24 773.1 0 24 74 43 773.1 0 43 75 118 773.1 0 118 76 188 773.1 0 188 77 165 773.1 0 165 78 67 773.1 0 67 79 22 773.1 0 22 80 30 773.1 0 30 81 42 773.1 0 42 82 36 773.1 0 36 83 28 773.1 0 28 84 39 773.1 0 39 85 32 773.1 0 32 86 43 773.1 0 43 87 34 773.1 0 34 88 57 773.1 0 57 89 62 773.1 0 62 90 67 773.1 0 67 91 72 773.1 0 72 92 64 773.1 0 64 93 65 773.1 0 65 94 59 773.1 0 59 95 54 773.1 0 54 96 53 773.1 0 53 97 43 773.1 0 43 98 56 773.1 0 56 99 68 773.1 0 68 100 72 773.1 0 72 101 49 773.1 0 49 102 61 773.1 0 61 103 67 773.1 0 67 104 49 773.1 0 49 105 45 773.1 0 45 106 59 773.1 0 59 107 60 773.1 0 60 108 50 773.1 0 50 109 43 773.1 0 43 110 52 773.1 0 52 111 55 773.1 0 55 112 78 773.1 0 78 113 1459 773.1 0 1459 114 751 773.1 0 751 115 843 773.1 0 843 116 89 773.1 0 89 117 63 773.1 0 63 118 48 773.1 0 48 119 34 773.1 0 34 120 48 773.1 0 48 121 110 773.1 0 110 122 87 773.1 0 87 123 98 773.1 0 98 124 87 773.1 0 87 125 56 773.1 0 56 126 98 773.1 0 98 127 66 773.1 0 66 128 150 773.1 0 150 129 337 773.1 0 337 130 76 773.1 0 76 131 52 773.1 0 52 132 71 773.1 0 71 133 111 773.1 0 111 134 144 773.1 0 144 135 89 773.1 0 89 136 105 773.1 0 105 137 106 773.1 0 106 138 91 773.1 0 91 139 114 773.1 0 114 140 105 773.1 0 105 141 118 773.1 0 118 142 223 773.1 0 223 143 176 773.1 0 176 144 284 773.1 0 284 145 205 773.1 0 205 146 267 773.1 0 267 147 5411 773.1 0 5411 148 4556 773.1 0 4556 149 5177 773.1 0 5177 150 425 773.1 0 425 151 57 773.1 0 57 152 54 773.1 0 54 153 110 773.1 0 110 154 75 773.1 0 75 155 117 773.1 0 117 156 105 773.1 0 105 157 104 773.1 0 104 158 100 773.1 0 100 159 72 773.1 0 72 160 87 773.1 0 87 161 122 773.1 0 122 162 113 773.1 0 113 163 102 773.1 0 102 164 82 773.1 0 82 165 96 773.1 0 96 166 50 773.1 0 50 167 50 773.1 0 50 168 112 773.1 0 112 169 113 773.1 0 113 170 89 773.1 0 89 171 60 773.1 0 60 172 66 773.1 0 66 173 121 773.1 0 121 174 112 773.1 0 112 175 127 773.1 0 127 176 143 773.1 0 143 177 99 773.1 0 99 178 112 773.1 0 112 179 145 773.1 0 145 180 154 773.1 0 154 181 196 773.1 0 196 182 178 773.1 0 178 183 165 773.1 0 165 184 134 773.1 0 134 185 193 773.1 0 193 186 253 773.1 0 253 187 377 773.1 0 377 188 281 773.1 0 281 189 1224 773.1 0 1224 190 1291 773.1 0 1291 191 1344 773.1 0 1344 192 989 773.1 0 989 193 560 773.1 0 560 194 514 773.1 0 514 195 800 773.1 0 800 196 680 773.1 0 680 197 815 773.1 0 815 198 1254 773.1 0 1254 199 5337 773.1 0 5337 200 31697 773.1 0 31697 201 20375 773.1 0 20375 202 29049 773.1 0 29049 203 3270 773.1 0 3270 204 3161 773.1 0 3161 205 56863 773.1 0 56863 206 54173 773.1 0 54173 207 73612 773.1 0 73612 208 21838 773.1 0 21838 209 6531 773.1 0 6531 210 1819 773.1 0 1819 211 2477 773.1 0 2477 212 1387 773.1 0 1387 213 1751 773.1 0 1751 214 960 773.1 0 960 215 2127 773.1 0 2127 216 1288 773.1 0 1288 217 2114 773.1 0 2114 218 1441 773.1 0 1441 219 6912 773.1 0 6912 220 5654 773.1 0 5654 221 6181 773.1 0 6181 222 4966 773.1 0 4966 223 3063 773.1 0 3063 224 2658 773.1 0 2658 225 20808 773.1 0 20808 226 41905 773.1 0 41905 227 40143 773.1 0 40143 228 28747 773.1 0 28747 229 129 773.1 0 129 230 68 773.1 0 68 231 117 773.1 0 117 232 61 773.1 0 61 233 37 773.1 0 37 234 64 773.1 0 64 235 108 773.1 0 108 236 108 773.1 0 108 237 88 773.1 0 88 238 162 773.1 0 162 239 81 773.1 0 81 240 75 773.1 0 75 241 54 773.1 0 54 242 71 773.1 0 71 243 78 773.1 0 78 244 434 773.1 0 434 245 430 773.1 0 430 246 680 773.1 0 680 247 447 773.1 0 447 248 749 773.1 0 749 249 1370 773.1 0 1370 250 337 773.1 0 337 251 720 773.1 0 720 252 511 773.1 0 511 253 488 773.1 0 488 254 313 773.1 0 313 255 522 773.1 0 522 256 366 773.1 0 366 257 529 773.1 0 529 258 611 773.1 0 611 259 1637 773.1 0 1637 260 1829 773.1 0 1829 261 2286 773.1 0 2286 262 44915 773.1 0 44915 263 35615 773.1 0 35615 264 58670 773.1 0 58670 265 540 773.1 0 540 266 157 773.1 0 157 267 61 773.1 0 61 268 65 773.1 0 65 269 113 773.1 0 113 270 255 773.1 0 255 271 182 773.1 0 182 272 52 773.1 0 52 273 102 773.1 0 102 274 85 773.1 0 85 275 146 773.1 0 146 276 122 773.1 0 122 277 124 773.1 0 124 278 143 773.1 0 143 279 67 773.1 0 67 280 53 773.1 0 53 281 62 773.1 0 62 282 129 773.1 0 129 283 351 773.1 0 351 284 493 773.1 0 493 285 194 773.1 0 194 286 72 773.1 0 72 287 59 773.1 0 59 288 55 773.1 0 55 289 113 773.1 0 113 290 77 773.1 0 77 291 106 773.1 0 106 292 1258 773.1 0 1258 293 926 773.1 0 926 294 1634 773.1 0 1634 295 216 773.1 0 216 296 146 773.1 0 146 297 173 773.1 0 173 298 170 773.1 0 170 299 76 773.1 0 76 300 100 773.1 0 100 301 145 773.1 0 145 Finished in 7.41 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 130,874 Reads with adapters: 15,077 (11.5%) Reads that were too short: 72 (0.1%) Reads written (passing filters): 15,005 (11.5%) Total basepairs processed: 21,499,940 bp Total written (filtered): 3,984,002 bp (18.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15077 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 8 19 210 0.0 3 0 181 21 4 4 20 13537 0.0 4 12878 537 95 18 9 21 39 0.0 4 18 17 2 2 22 6 0.0 4 3 1 1 1 23 23 0.0 4 0 0 0 15 8 27 1 0.0 4 1 30 1 0.0 4 1 36 4 0.0 4 0 0 0 1 3 39 1 0.0 4 0 0 0 0 1 64 4 0.0 4 0 0 0 0 4 65 1 0.0 4 0 0 0 0 1 66 3 0.0 4 0 0 0 0 3 86 1 0.0 4 0 0 0 0 1 91 6 0.0 4 0 1 0 0 5 92 11 0.0 4 0 0 0 1 10 94 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 104 7 0.0 4 0 0 0 0 7 105 4 0.0 4 0 0 0 0 4 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 16 0.0 4 0 0 0 0 16 127 1153 0.0 4 0 0 0 0 1153 128 7 0.0 4 0 0 0 0 7 129 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 139 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 1 228 11 0.0 4 0 0 0 0 11 229 4 0.0 4 0 0 0 0 4 234 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.77 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 15,005 Reads with adapters: 528 (3.5%) Reads written (passing filters): 15,005 (100.0%) Total basepairs processed: 3,984,002 bp Total written (filtered): 3,977,121 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 528 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.9% C: 12.3% G: 72.9% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 73 234.5 0 73 4 12 58.6 0 12 7 1 0.9 0 1 9 4 0.1 0 1 3 10 4 0.0 1 0 4 11 205 0.0 1 4 201 12 206 0.0 1 5 201 13 7 0.0 1 0 7 14 1 0.0 1 1 39 7 0.0 1 4 3 124 2 0.0 1 2 152 1 0.0 1 1 171 2 0.0 1 2 209 1 0.0 1 0 1 211 1 0.0 1 0 1 264 1 0.0 1 1