This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 139.38 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 143,310 (4.5%) Reads that were too short: 35,084 (1.1%) Reads written (passing filters): 108,226 (3.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 26,002,128 bp (2.7%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 143310 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 560 773.1 0 560 7 2930 773.1 0 2930 8 31551 773.1 0 31551 9 28182 773.1 0 28182 10 50 773.1 0 50 11 27 773.1 0 27 12 48 773.1 0 48 13 18 773.1 0 18 14 25 773.1 0 25 15 16 773.1 0 16 16 19 773.1 0 19 17 29 773.1 0 29 18 22 773.1 0 22 19 22 773.1 0 22 20 22 773.1 0 22 21 12 773.1 0 12 22 20 773.1 0 20 23 40 773.1 0 40 24 19 773.1 0 19 25 33 773.1 0 33 26 27 773.1 0 27 27 32 773.1 0 32 28 14 773.1 0 14 29 29 773.1 0 29 30 27 773.1 0 27 31 27 773.1 0 27 32 61 773.1 0 61 33 67 773.1 0 67 34 75 773.1 0 75 35 37 773.1 0 37 36 47 773.1 0 47 37 204 773.1 0 204 38 217 773.1 0 217 39 336 773.1 0 336 40 61 773.1 0 61 41 83 773.1 0 83 42 164 773.1 0 164 43 17 773.1 0 17 44 34 773.1 0 34 45 23 773.1 0 23 46 21 773.1 0 21 47 41 773.1 0 41 48 47 773.1 0 47 49 37 773.1 0 37 50 25 773.1 0 25 51 36 773.1 0 36 52 513 773.1 0 513 53 247 773.1 0 247 54 325 773.1 0 325 55 24 773.1 0 24 56 21 773.1 0 21 57 35 773.1 0 35 58 28 773.1 0 28 59 23 773.1 0 23 60 37 773.1 0 37 61 58 773.1 0 58 62 96 773.1 0 96 63 25 773.1 0 25 64 12 773.1 0 12 65 45 773.1 0 45 66 71 773.1 0 71 67 60 773.1 0 60 68 77 773.1 0 77 69 21 773.1 0 21 70 24 773.1 0 24 71 82 773.1 0 82 72 157 773.1 0 157 73 147 773.1 0 147 74 16 773.1 0 16 75 22 773.1 0 22 76 13 773.1 0 13 77 22 773.1 0 22 78 27 773.1 0 27 79 16 773.1 0 16 80 19 773.1 0 19 81 38 773.1 0 38 82 31 773.1 0 31 83 26 773.1 0 26 84 76 773.1 0 76 85 65 773.1 0 65 86 145 773.1 0 145 87 34 773.1 0 34 88 18 773.1 0 18 89 31 773.1 0 31 90 105 773.1 0 105 91 122 773.1 0 122 92 90 773.1 0 90 93 105 773.1 0 105 94 90 773.1 0 90 95 120 773.1 0 120 96 35 773.1 0 35 97 71 773.1 0 71 98 33 773.1 0 33 99 17 773.1 0 17 100 18 773.1 0 18 101 25 773.1 0 25 102 21 773.1 0 21 103 33 773.1 0 33 104 64 773.1 0 64 105 65 773.1 0 65 106 58 773.1 0 58 107 89 773.1 0 89 108 329 773.1 0 329 109 143 773.1 0 143 110 386 773.1 0 386 111 168 773.1 0 168 112 168 773.1 0 168 113 148 773.1 0 148 114 35 773.1 0 35 115 39 773.1 0 39 116 32 773.1 0 32 117 103 773.1 0 103 118 77 773.1 0 77 119 101 773.1 0 101 120 23 773.1 0 23 121 22 773.1 0 22 122 34 773.1 0 34 123 17 773.1 0 17 124 47 773.1 0 47 125 51 773.1 0 51 126 78 773.1 0 78 127 507 773.1 0 507 128 180 773.1 0 180 129 377 773.1 0 377 130 40 773.1 0 40 131 54 773.1 0 54 132 128 773.1 0 128 133 68 773.1 0 68 134 60 773.1 0 60 135 37 773.1 0 37 136 35 773.1 0 35 137 38 773.1 0 38 138 72 773.1 0 72 139 185 773.1 0 185 140 162 773.1 0 162 141 244 773.1 0 244 142 358 773.1 0 358 143 673 773.1 0 673 144 11053 773.1 0 11053 145 7922 773.1 0 7922 146 9545 773.1 0 9545 147 92 773.1 0 92 148 36 773.1 0 36 149 124 773.1 0 124 150 54 773.1 0 54 151 255 773.1 0 255 152 178 773.1 0 178 153 422 773.1 0 422 154 170 773.1 0 170 155 68 773.1 0 68 156 226 773.1 0 226 157 215 773.1 0 215 158 322 773.1 0 322 159 63 773.1 0 63 160 36 773.1 0 36 161 65 773.1 0 65 162 29 773.1 0 29 163 33 773.1 0 33 164 31 773.1 0 31 165 44 773.1 0 44 166 39 773.1 0 39 167 21 773.1 0 21 168 22 773.1 0 22 169 25 773.1 0 25 170 48 773.1 0 48 171 66 773.1 0 66 172 79 773.1 0 79 173 69 773.1 0 69 174 52 773.1 0 52 175 35 773.1 0 35 176 42 773.1 0 42 177 60 773.1 0 60 178 68 773.1 0 68 179 258 773.1 0 258 180 211 773.1 0 211 181 153 773.1 0 153 182 26 773.1 0 26 183 37 773.1 0 37 184 36 773.1 0 36 185 47 773.1 0 47 186 56 773.1 0 56 187 51 773.1 0 51 188 52 773.1 0 52 189 31 773.1 0 31 190 302 773.1 0 302 191 78 773.1 0 78 192 94 773.1 0 94 193 55 773.1 0 55 194 40 773.1 0 40 195 45 773.1 0 45 196 48 773.1 0 48 197 64 773.1 0 64 198 164 773.1 0 164 199 102 773.1 0 102 200 63 773.1 0 63 201 63 773.1 0 63 202 40 773.1 0 40 203 46 773.1 0 46 204 73 773.1 0 73 205 48 773.1 0 48 206 118 773.1 0 118 207 69 773.1 0 69 208 43 773.1 0 43 209 25 773.1 0 25 210 21 773.1 0 21 211 26 773.1 0 26 212 20 773.1 0 20 213 28 773.1 0 28 214 37 773.1 0 37 215 37 773.1 0 37 216 29 773.1 0 29 217 40 773.1 0 40 218 50 773.1 0 50 219 111 773.1 0 111 220 34 773.1 0 34 221 39 773.1 0 39 222 27 773.1 0 27 223 31 773.1 0 31 224 34 773.1 0 34 225 40 773.1 0 40 226 61 773.1 0 61 227 57 773.1 0 57 228 93 773.1 0 93 229 117 773.1 0 117 230 154 773.1 0 154 231 160 773.1 0 160 232 112 773.1 0 112 233 62 773.1 0 62 234 78 773.1 0 78 235 386 773.1 0 386 236 585 773.1 0 585 237 614 773.1 0 614 238 497 773.1 0 497 239 114 773.1 0 114 240 336 773.1 0 336 241 323 773.1 0 323 242 294 773.1 0 294 243 77 773.1 0 77 244 61 773.1 0 61 245 191 773.1 0 191 246 188 773.1 0 188 247 405 773.1 0 405 248 183 773.1 0 183 249 221 773.1 0 221 250 321 773.1 0 321 251 272 773.1 0 272 252 336 773.1 0 336 253 125 773.1 0 125 254 45 773.1 0 45 255 58 773.1 0 58 256 81 773.1 0 81 257 96 773.1 0 96 258 196 773.1 0 196 259 369 773.1 0 369 260 4511 773.1 0 4511 261 5560 773.1 0 5560 262 5711 773.1 0 5711 263 2609 773.1 0 2609 264 66 773.1 0 66 265 71 773.1 0 71 266 109 773.1 0 109 267 146 773.1 0 146 268 153 773.1 0 153 269 94 773.1 0 94 270 241 773.1 0 241 271 253 773.1 0 253 272 250 773.1 0 250 273 58 773.1 0 58 274 44 773.1 0 44 275 47 773.1 0 47 276 64 773.1 0 64 277 135 773.1 0 135 278 121 773.1 0 121 279 242 773.1 0 242 280 259 773.1 0 259 281 245 773.1 0 245 282 257 773.1 0 257 283 181 773.1 0 181 284 473 773.1 0 473 285 124 773.1 0 124 286 260 773.1 0 260 287 115 773.1 0 115 288 310 773.1 0 310 289 246 773.1 0 246 290 271 773.1 0 271 291 469 773.1 0 469 292 588 773.1 0 588 293 830 773.1 0 830 294 634 773.1 0 634 295 379 773.1 0 379 296 286 773.1 0 286 297 99 773.1 0 99 298 70 773.1 0 70 299 50 773.1 0 50 300 48 773.1 0 48 301 71 773.1 0 71 Finished in 7.34 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 108,226 Reads with adapters: 30,924 (28.6%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 30,894 (28.5%) Total basepairs processed: 26,002,128 bp Total written (filtered): 8,250,293 bp (31.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30924 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 17 0.0 3 0 0 12 5 19 431 0.0 3 0 378 28 19 6 20 28811 0.0 4 27400 1126 209 60 16 21 68 0.0 4 7 58 2 1 22 5 0.0 4 0 1 2 1 1 23 95 0.0 4 0 0 0 73 22 24 3 0.0 4 0 0 0 0 3 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 65 3 0.0 4 0 0 0 1 2 122 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 13 0.0 4 0 0 0 0 13 127 1430 0.0 4 0 0 0 0 1430 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 1 130 3 0.0 4 0 0 0 0 3 144 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 164 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 229 5 0.0 4 0 0 0 0 5 230 2 0.0 4 0 0 0 0 2 233 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 1 265 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.76 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 30,894 Reads with adapters: 6,618 (21.4%) Reads written (passing filters): 30,894 (100.0%) Total basepairs processed: 8,250,293 bp Total written (filtered): 8,183,187 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6618 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 5.6% G: 91.1% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 482.7 0 14 4 6 120.7 0 6 6 1 7.5 0 1 7 2 1.9 0 2 8 3 0.5 0 3 9 2013 0.1 0 149 1864 10 3707 0.0 1 203 3504 11 835 0.0 1 15 820 12 14 0.0 1 0 14 13 7 0.0 1 0 7 14 1 0.0 1 0 1 27 1 0.0 1 1 34 1 0.0 1 1 39 1 0.0 1 1 62 1 0.0 1 1 152 1 0.0 1 1 169 2 0.0 1 2 208 6 0.0 1 3 3 210 1 0.0 1 1 241 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.