This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.12 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 128,613 (4.1%) Reads that were too short: 18,618 (0.6%) Reads written (passing filters): 109,995 (3.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 26,352,346 bp (2.8%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 128613 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 326 773.1 0 326 7 2077 773.1 0 2077 8 28893 773.1 0 28893 9 34176 773.1 0 34176 10 45 773.1 0 45 11 9 773.1 0 9 12 7 773.1 0 7 13 11 773.1 0 11 14 7 773.1 0 7 15 6 773.1 0 6 16 8 773.1 0 8 17 8 773.1 0 8 18 11 773.1 0 11 19 9 773.1 0 9 20 8 773.1 0 8 21 9 773.1 0 9 22 12 773.1 0 12 23 14 773.1 0 14 24 11 773.1 0 11 25 17 773.1 0 17 26 8 773.1 0 8 27 9 773.1 0 9 28 9 773.1 0 9 29 16 773.1 0 16 30 11 773.1 0 11 31 12 773.1 0 12 32 23 773.1 0 23 33 64 773.1 0 64 34 67 773.1 0 67 35 16 773.1 0 16 36 29 773.1 0 29 37 160 773.1 0 160 38 124 773.1 0 124 39 150 773.1 0 150 40 16 773.1 0 16 41 17 773.1 0 17 42 34 773.1 0 34 43 16 773.1 0 16 44 10 773.1 0 10 45 12 773.1 0 12 46 14 773.1 0 14 47 21 773.1 0 21 48 14 773.1 0 14 49 16 773.1 0 16 50 12 773.1 0 12 51 25 773.1 0 25 52 207 773.1 0 207 53 154 773.1 0 154 54 164 773.1 0 164 55 12 773.1 0 12 56 8 773.1 0 8 57 7 773.1 0 7 58 21 773.1 0 21 59 9 773.1 0 9 60 164 773.1 0 164 61 91 773.1 0 91 62 55 773.1 0 55 63 16 773.1 0 16 64 9 773.1 0 9 65 17 773.1 0 17 66 17 773.1 0 17 67 15 773.1 0 15 68 18 773.1 0 18 69 11 773.1 0 11 70 13 773.1 0 13 71 29 773.1 0 29 72 62 773.1 0 62 73 91 773.1 0 91 74 13 773.1 0 13 75 15 773.1 0 15 76 6 773.1 0 6 77 14 773.1 0 14 78 13 773.1 0 13 79 10 773.1 0 10 80 17 773.1 0 17 81 22 773.1 0 22 82 19 773.1 0 19 83 18 773.1 0 18 84 118 773.1 0 118 85 34 773.1 0 34 86 110 773.1 0 110 87 24 773.1 0 24 88 14 773.1 0 14 89 20 773.1 0 20 90 50 773.1 0 50 91 39 773.1 0 39 92 49 773.1 0 49 93 25 773.1 0 25 94 19 773.1 0 19 95 32 773.1 0 32 96 16 773.1 0 16 97 69 773.1 0 69 98 27 773.1 0 27 99 14 773.1 0 14 100 23 773.1 0 23 101 27 773.1 0 27 102 10 773.1 0 10 103 31 773.1 0 31 104 33 773.1 0 33 105 39 773.1 0 39 106 32 773.1 0 32 107 40 773.1 0 40 108 107 773.1 0 107 109 63 773.1 0 63 110 115 773.1 0 115 111 54 773.1 0 54 112 67 773.1 0 67 113 67 773.1 0 67 114 22 773.1 0 22 115 22 773.1 0 22 116 24 773.1 0 24 117 70 773.1 0 70 118 27 773.1 0 27 119 60 773.1 0 60 120 13 773.1 0 13 121 24 773.1 0 24 122 16 773.1 0 16 123 20 773.1 0 20 124 38 773.1 0 38 125 46 773.1 0 46 126 87 773.1 0 87 127 409 773.1 0 409 128 116 773.1 0 116 129 177 773.1 0 177 130 29 773.1 0 29 131 46 773.1 0 46 132 129 773.1 0 129 133 33 773.1 0 33 134 36 773.1 0 36 135 23 773.1 0 23 136 30 773.1 0 30 137 57 773.1 0 57 138 58 773.1 0 58 139 123 773.1 0 123 140 161 773.1 0 161 141 230 773.1 0 230 142 407 773.1 0 407 143 666 773.1 0 666 144 13590 773.1 0 13590 145 9937 773.1 0 9937 146 11364 773.1 0 11364 147 77 773.1 0 77 148 27 773.1 0 27 149 80 773.1 0 80 150 20 773.1 0 20 151 102 773.1 0 102 152 81 773.1 0 81 153 100 773.1 0 100 154 53 773.1 0 53 155 18 773.1 0 18 156 102 773.1 0 102 157 79 773.1 0 79 158 156 773.1 0 156 159 20 773.1 0 20 160 13 773.1 0 13 161 22 773.1 0 22 162 16 773.1 0 16 163 20 773.1 0 20 164 9 773.1 0 9 165 11 773.1 0 11 166 26 773.1 0 26 167 10 773.1 0 10 168 16 773.1 0 16 169 7 773.1 0 7 170 13 773.1 0 13 171 29 773.1 0 29 172 36 773.1 0 36 173 20 773.1 0 20 174 21 773.1 0 21 175 17 773.1 0 17 176 16 773.1 0 16 177 23 773.1 0 23 178 27 773.1 0 27 179 131 773.1 0 131 180 112 773.1 0 112 181 81 773.1 0 81 182 13 773.1 0 13 183 18 773.1 0 18 184 18 773.1 0 18 185 20 773.1 0 20 186 32 773.1 0 32 187 25 773.1 0 25 188 54 773.1 0 54 189 28 773.1 0 28 190 427 773.1 0 427 191 34 773.1 0 34 192 78 773.1 0 78 193 14 773.1 0 14 194 31 773.1 0 31 195 17 773.1 0 17 196 16 773.1 0 16 197 33 773.1 0 33 198 77 773.1 0 77 199 44 773.1 0 44 200 34 773.1 0 34 201 27 773.1 0 27 202 18 773.1 0 18 203 23 773.1 0 23 204 27 773.1 0 27 205 25 773.1 0 25 206 34 773.1 0 34 207 23 773.1 0 23 208 16 773.1 0 16 209 10 773.1 0 10 210 16 773.1 0 16 211 14 773.1 0 14 212 9 773.1 0 9 213 19 773.1 0 19 214 27 773.1 0 27 215 19 773.1 0 19 216 22 773.1 0 22 217 25 773.1 0 25 218 40 773.1 0 40 219 102 773.1 0 102 220 17 773.1 0 17 221 34 773.1 0 34 222 15 773.1 0 15 223 12 773.1 0 12 224 18 773.1 0 18 225 15 773.1 0 15 226 23 773.1 0 23 227 30 773.1 0 30 228 41 773.1 0 41 229 62 773.1 0 62 230 107 773.1 0 107 231 133 773.1 0 133 232 47 773.1 0 47 233 34 773.1 0 34 234 35 773.1 0 35 235 270 773.1 0 270 236 252 773.1 0 252 237 311 773.1 0 311 238 193 773.1 0 193 239 59 773.1 0 59 240 195 773.1 0 195 241 139 773.1 0 139 242 110 773.1 0 110 243 30 773.1 0 30 244 24 773.1 0 24 245 87 773.1 0 87 246 78 773.1 0 78 247 171 773.1 0 171 248 83 773.1 0 83 249 79 773.1 0 79 250 118 773.1 0 118 251 118 773.1 0 118 252 118 773.1 0 118 253 51 773.1 0 51 254 26 773.1 0 26 255 25 773.1 0 25 256 43 773.1 0 43 257 51 773.1 0 51 258 87 773.1 0 87 259 221 773.1 0 221 260 2442 773.1 0 2442 261 3543 773.1 0 3543 262 3327 773.1 0 3327 263 1521 773.1 0 1521 264 39 773.1 0 39 265 22 773.1 0 22 266 38 773.1 0 38 267 70 773.1 0 70 268 73 773.1 0 73 269 54 773.1 0 54 270 154 773.1 0 154 271 171 773.1 0 171 272 101 773.1 0 101 273 25 773.1 0 25 274 20 773.1 0 20 275 24 773.1 0 24 276 32 773.1 0 32 277 79 773.1 0 79 278 68 773.1 0 68 279 123 773.1 0 123 280 120 773.1 0 120 281 108 773.1 0 108 282 76 773.1 0 76 283 143 773.1 0 143 284 542 773.1 0 542 285 117 773.1 0 117 286 162 773.1 0 162 287 38 773.1 0 38 288 159 773.1 0 159 289 118 773.1 0 118 290 108 773.1 0 108 291 120 773.1 0 120 292 139 773.1 0 139 293 213 773.1 0 213 294 139 773.1 0 139 295 115 773.1 0 115 296 96 773.1 0 96 297 37 773.1 0 37 298 24 773.1 0 24 299 24 773.1 0 24 300 22 773.1 0 22 301 41 773.1 0 41 Finished in 6.91 s (63 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 109,995 Reads with adapters: 29,863 (27.1%) Reads that were too short: 70 (0.1%) Reads written (passing filters): 29,793 (27.1%) Total basepairs processed: 26,352,346 bp Total written (filtered): 8,222,741 bp (31.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 29863 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 5 0.0 3 0 0 5 16 178 0.0 3 0 175 2 1 17 29493 0.0 3 29005 464 20 4 18 61 0.0 3 1 57 2 1 19 25 0.0 3 5 0 17 3 20 19 0.0 3 0 0 0 19 21 1 0.0 3 1 44 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 59 1 0.0 3 1 61 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 143 1 0.0 3 0 1 149 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 217 57 0.0 3 0 0 1 56 234 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.69 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 29,793 Reads with adapters: 238 (0.8%) Reads written (passing filters): 29,793 (100.0%) Total basepairs processed: 8,222,741 bp Total written (filtered): 8,221,936 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 238 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.7% C: 5.9% G: 26.1% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 152 465.5 0 152 4 83 116.4 0 83 5 2 29.1 0 2 7 1 1.8 0 1