This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 139.32 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 94,955 (3.0%) Reads that were too short: 13,611 (0.4%) Reads written (passing filters): 81,344 (2.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 21,592,246 bp (2.3%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 94955 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 620 773.1 0 620 7 34797 773.1 0 34797 8 25586 773.1 0 25586 9 54 773.1 0 54 10 41 773.1 0 41 11 41 773.1 0 41 12 30 773.1 0 30 13 41 773.1 0 41 14 31 773.1 0 31 15 29 773.1 0 29 16 34 773.1 0 34 17 32 773.1 0 32 18 145 773.1 0 145 19 59 773.1 0 59 20 97 773.1 0 97 21 31 773.1 0 31 22 35 773.1 0 35 23 35 773.1 0 35 24 48 773.1 0 48 25 50 773.1 0 50 26 48 773.1 0 48 27 56 773.1 0 56 28 34 773.1 0 34 29 26 773.1 0 26 30 38 773.1 0 38 31 36 773.1 0 36 32 42 773.1 0 42 33 72 773.1 0 72 34 44 773.1 0 44 35 48 773.1 0 48 36 40 773.1 0 40 37 41 773.1 0 41 38 40 773.1 0 40 39 55 773.1 0 55 40 38 773.1 0 38 41 49 773.1 0 49 42 39 773.1 0 39 43 32 773.1 0 32 44 50 773.1 0 50 45 34 773.1 0 34 46 36 773.1 0 36 47 96 773.1 0 96 48 34 773.1 0 34 49 42 773.1 0 42 50 44 773.1 0 44 51 58 773.1 0 58 52 34 773.1 0 34 53 44 773.1 0 44 54 51 773.1 0 51 55 54 773.1 0 54 56 40 773.1 0 40 57 39 773.1 0 39 58 43 773.1 0 43 59 55 773.1 0 55 60 42 773.1 0 42 61 43 773.1 0 43 62 47 773.1 0 47 63 36 773.1 0 36 64 24 773.1 0 24 65 51 773.1 0 51 66 80 773.1 0 80 67 81 773.1 0 81 68 57 773.1 0 57 69 89 773.1 0 89 70 85 773.1 0 85 71 816 773.1 0 816 72 981 773.1 0 981 73 995 773.1 0 995 74 44 773.1 0 44 75 52 773.1 0 52 76 36 773.1 0 36 77 79 773.1 0 79 78 60 773.1 0 60 79 104 773.1 0 104 80 51 773.1 0 51 81 43 773.1 0 43 82 53 773.1 0 53 83 45 773.1 0 45 84 52 773.1 0 52 85 67 773.1 0 67 86 60 773.1 0 60 87 63 773.1 0 63 88 49 773.1 0 49 89 36 773.1 0 36 90 58 773.1 0 58 91 62 773.1 0 62 92 52 773.1 0 52 93 66 773.1 0 66 94 91 773.1 0 91 95 111 773.1 0 111 96 763 773.1 0 763 97 785 773.1 0 785 98 545 773.1 0 545 99 188 773.1 0 188 100 34 773.1 0 34 101 30 773.1 0 30 102 53 773.1 0 53 103 53 773.1 0 53 104 49 773.1 0 49 105 56 773.1 0 56 106 46 773.1 0 46 107 61 773.1 0 61 108 85 773.1 0 85 109 58 773.1 0 58 110 52 773.1 0 52 111 38 773.1 0 38 112 47 773.1 0 47 113 49 773.1 0 49 114 71 773.1 0 71 115 59 773.1 0 59 116 52 773.1 0 52 117 55 773.1 0 55 118 84 773.1 0 84 119 49 773.1 0 49 120 50 773.1 0 50 121 54 773.1 0 54 122 50 773.1 0 50 123 57 773.1 0 57 124 94 773.1 0 94 125 132 773.1 0 132 126 132 773.1 0 132 127 117 773.1 0 117 128 90 773.1 0 90 129 118 773.1 0 118 130 131 773.1 0 131 131 79 773.1 0 79 132 80 773.1 0 80 133 73 773.1 0 73 134 89 773.1 0 89 135 131 773.1 0 131 136 67 773.1 0 67 137 74 773.1 0 74 138 67 773.1 0 67 139 76 773.1 0 76 140 126 773.1 0 126 141 116 773.1 0 116 142 77 773.1 0 77 143 195 773.1 0 195 144 252 773.1 0 252 145 67 773.1 0 67 146 68 773.1 0 68 147 54 773.1 0 54 148 85 773.1 0 85 149 53 773.1 0 53 150 70 773.1 0 70 151 107 773.1 0 107 152 70 773.1 0 70 153 51 773.1 0 51 154 78 773.1 0 78 155 73 773.1 0 73 156 79 773.1 0 79 157 54 773.1 0 54 158 53 773.1 0 53 159 52 773.1 0 52 160 60 773.1 0 60 161 65 773.1 0 65 162 51 773.1 0 51 163 51 773.1 0 51 164 75 773.1 0 75 165 93 773.1 0 93 166 61 773.1 0 61 167 68 773.1 0 68 168 68 773.1 0 68 169 54 773.1 0 54 170 56 773.1 0 56 171 78 773.1 0 78 172 103 773.1 0 103 173 260 773.1 0 260 174 229 773.1 0 229 175 315 773.1 0 315 176 90 773.1 0 90 177 57 773.1 0 57 178 62 773.1 0 62 179 59 773.1 0 59 180 92 773.1 0 92 181 59 773.1 0 59 182 55 773.1 0 55 183 55 773.1 0 55 184 85 773.1 0 85 185 85 773.1 0 85 186 109 773.1 0 109 187 72 773.1 0 72 188 71 773.1 0 71 189 63 773.1 0 63 190 108 773.1 0 108 191 181 773.1 0 181 192 672 773.1 0 672 193 928 773.1 0 928 194 838 773.1 0 838 195 449 773.1 0 449 196 60 773.1 0 60 197 58 773.1 0 58 198 78 773.1 0 78 199 98 773.1 0 98 200 74 773.1 0 74 201 67 773.1 0 67 202 37 773.1 0 37 203 68 773.1 0 68 204 94 773.1 0 94 205 89 773.1 0 89 206 90 773.1 0 90 207 94 773.1 0 94 208 68 773.1 0 68 209 112 773.1 0 112 210 126 773.1 0 126 211 128 773.1 0 128 212 100 773.1 0 100 213 81 773.1 0 81 214 57 773.1 0 57 215 139 773.1 0 139 216 138 773.1 0 138 217 197 773.1 0 197 218 64 773.1 0 64 219 135 773.1 0 135 220 181 773.1 0 181 221 148 773.1 0 148 222 77 773.1 0 77 223 58 773.1 0 58 224 55 773.1 0 55 225 90 773.1 0 90 226 153 773.1 0 153 227 141 773.1 0 141 228 88 773.1 0 88 229 61 773.1 0 61 230 64 773.1 0 64 231 78 773.1 0 78 232 69 773.1 0 69 233 80 773.1 0 80 234 72 773.1 0 72 235 67 773.1 0 67 236 93 773.1 0 93 237 103 773.1 0 103 238 91 773.1 0 91 239 82 773.1 0 82 240 127 773.1 0 127 241 120 773.1 0 120 242 106 773.1 0 106 243 61 773.1 0 61 244 54 773.1 0 54 245 103 773.1 0 103 246 104 773.1 0 104 247 140 773.1 0 140 248 88 773.1 0 88 249 126 773.1 0 126 250 96 773.1 0 96 251 70 773.1 0 70 252 73 773.1 0 73 253 71 773.1 0 71 254 143 773.1 0 143 255 159 773.1 0 159 256 128 773.1 0 128 257 94 773.1 0 94 258 97 773.1 0 97 259 114 773.1 0 114 260 110 773.1 0 110 261 109 773.1 0 109 262 79 773.1 0 79 263 124 773.1 0 124 264 92 773.1 0 92 265 97 773.1 0 97 266 119 773.1 0 119 267 147 773.1 0 147 268 129 773.1 0 129 269 98 773.1 0 98 270 188 773.1 0 188 271 219 773.1 0 219 272 1065 773.1 0 1065 273 719 773.1 0 719 274 1110 773.1 0 1110 275 107 773.1 0 107 276 96 773.1 0 96 277 102 773.1 0 102 278 135 773.1 0 135 279 130 773.1 0 130 280 194 773.1 0 194 281 154 773.1 0 154 282 118 773.1 0 118 283 83 773.1 0 83 284 83 773.1 0 83 285 103 773.1 0 103 286 114 773.1 0 114 287 138 773.1 0 138 288 173 773.1 0 173 289 176 773.1 0 176 290 146 773.1 0 146 291 143 773.1 0 143 292 111 773.1 0 111 293 133 773.1 0 133 294 218 773.1 0 218 295 182 773.1 0 182 296 190 773.1 0 190 297 92 773.1 0 92 298 66 773.1 0 66 299 73 773.1 0 73 300 119 773.1 0 119 301 85 773.1 0 85 Finished in 5.89 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 81,344 Reads with adapters: 27,610 (33.9%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 27,584 (33.9%) Total basepairs processed: 21,592,246 bp Total written (filtered): 7,398,968 bp (34.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27610 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 18 0.0 3 0 0 14 4 19 434 0.0 3 0 379 32 17 6 20 25756 0.0 4 24508 1012 163 57 16 21 57 0.0 4 8 46 1 0 2 22 9 0.0 4 0 4 5 23 56 0.0 4 0 0 0 44 12 26 1 0.0 4 1 29 1 0.0 4 1 38 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 101 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 19 0.0 4 0 0 0 0 19 127 1203 0.0 4 0 0 0 0 1203 128 4 0.0 4 0 0 0 0 4 129 3 0.0 4 0 0 0 1 2 130 6 0.0 4 0 0 0 0 6 149 1 0.0 4 0 0 0 1 153 4 0.0 4 0 0 0 1 3 180 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 0 9 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 281 2 0.0 4 0 0 0 1 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.54 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 27,584 Reads with adapters: 5,376 (19.5%) Reads written (passing filters): 27,584 (100.0%) Total basepairs processed: 7,398,968 bp Total written (filtered): 7,337,925 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5376 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 4.7% G: 91.8% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 34 431.0 0 34 4 8 107.8 0 8 5 1 26.9 0 1 7 1 1.7 0 1 8 1 0.4 0 1 9 31 0.1 0 1 30 10 188 0.0 1 11 177 11 5074 0.0 1 114 4960 12 17 0.0 1 1 16 13 2 0.0 1 0 2 14 2 0.0 1 0 2 21 1 0.0 1 1 24 1 0.0 1 1 151 8 0.0 1 7 1 170 2 0.0 1 1 1 209 4 0.0 1 1 3 229 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.