This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 137.54 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 87,664 (2.8%) Reads that were too short: 7,801 (0.2%) Reads written (passing filters): 79,863 (2.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 21,532,668 bp (2.3%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 87664 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 502 773.1 0 502 7 31134 773.1 0 31134 8 30440 773.1 0 30440 9 51 773.1 0 51 10 27 773.1 0 27 11 24 773.1 0 24 12 17 773.1 0 17 13 36 773.1 0 36 14 28 773.1 0 28 15 22 773.1 0 22 16 28 773.1 0 28 17 25 773.1 0 25 18 64 773.1 0 64 19 46 773.1 0 46 20 67 773.1 0 67 21 38 773.1 0 38 22 44 773.1 0 44 23 23 773.1 0 23 24 28 773.1 0 28 25 31 773.1 0 31 26 43 773.1 0 43 27 28 773.1 0 28 28 39 773.1 0 39 29 22 773.1 0 22 30 50 773.1 0 50 31 35 773.1 0 35 32 45 773.1 0 45 33 78 773.1 0 78 34 37 773.1 0 37 35 48 773.1 0 48 36 39 773.1 0 39 37 40 773.1 0 40 38 37 773.1 0 37 39 27 773.1 0 27 40 44 773.1 0 44 41 49 773.1 0 49 42 55 773.1 0 55 43 25 773.1 0 25 44 38 773.1 0 38 45 47 773.1 0 47 46 46 773.1 0 46 47 99 773.1 0 99 48 59 773.1 0 59 49 38 773.1 0 38 50 32 773.1 0 32 51 66 773.1 0 66 52 32 773.1 0 32 53 33 773.1 0 33 54 51 773.1 0 51 55 31 773.1 0 31 56 33 773.1 0 33 57 40 773.1 0 40 58 38 773.1 0 38 59 58 773.1 0 58 60 26 773.1 0 26 61 35 773.1 0 35 62 37 773.1 0 37 63 38 773.1 0 38 64 52 773.1 0 52 65 36 773.1 0 36 66 52 773.1 0 52 67 55 773.1 0 55 68 63 773.1 0 63 69 81 773.1 0 81 70 89 773.1 0 89 71 918 773.1 0 918 72 1017 773.1 0 1017 73 1110 773.1 0 1110 74 43 773.1 0 43 75 28 773.1 0 28 76 47 773.1 0 47 77 80 773.1 0 80 78 57 773.1 0 57 79 105 773.1 0 105 80 37 773.1 0 37 81 23 773.1 0 23 82 39 773.1 0 39 83 31 773.1 0 31 84 39 773.1 0 39 85 38 773.1 0 38 86 44 773.1 0 44 87 61 773.1 0 61 88 28 773.1 0 28 89 68 773.1 0 68 90 50 773.1 0 50 91 48 773.1 0 48 92 45 773.1 0 45 93 62 773.1 0 62 94 72 773.1 0 72 95 93 773.1 0 93 96 831 773.1 0 831 97 977 773.1 0 977 98 575 773.1 0 575 99 261 773.1 0 261 100 42 773.1 0 42 101 28 773.1 0 28 102 34 773.1 0 34 103 47 773.1 0 47 104 45 773.1 0 45 105 55 773.1 0 55 106 54 773.1 0 54 107 40 773.1 0 40 108 66 773.1 0 66 109 62 773.1 0 62 110 37 773.1 0 37 111 29 773.1 0 29 112 43 773.1 0 43 113 38 773.1 0 38 114 45 773.1 0 45 115 61 773.1 0 61 116 49 773.1 0 49 117 50 773.1 0 50 118 69 773.1 0 69 119 49 773.1 0 49 120 37 773.1 0 37 121 39 773.1 0 39 122 34 773.1 0 34 123 40 773.1 0 40 124 54 773.1 0 54 125 104 773.1 0 104 126 96 773.1 0 96 127 64 773.1 0 64 128 53 773.1 0 53 129 68 773.1 0 68 130 87 773.1 0 87 131 48 773.1 0 48 132 55 773.1 0 55 133 50 773.1 0 50 134 56 773.1 0 56 135 98 773.1 0 98 136 46 773.1 0 46 137 48 773.1 0 48 138 46 773.1 0 46 139 47 773.1 0 47 140 84 773.1 0 84 141 97 773.1 0 97 142 57 773.1 0 57 143 180 773.1 0 180 144 225 773.1 0 225 145 39 773.1 0 39 146 58 773.1 0 58 147 47 773.1 0 47 148 74 773.1 0 74 149 35 773.1 0 35 150 53 773.1 0 53 151 101 773.1 0 101 152 58 773.1 0 58 153 31 773.1 0 31 154 52 773.1 0 52 155 56 773.1 0 56 156 57 773.1 0 57 157 49 773.1 0 49 158 46 773.1 0 46 159 45 773.1 0 45 160 56 773.1 0 56 161 43 773.1 0 43 162 34 773.1 0 34 163 37 773.1 0 37 164 44 773.1 0 44 165 64 773.1 0 64 166 39 773.1 0 39 167 48 773.1 0 48 168 41 773.1 0 41 169 43 773.1 0 43 170 49 773.1 0 49 171 49 773.1 0 49 172 76 773.1 0 76 173 140 773.1 0 140 174 114 773.1 0 114 175 172 773.1 0 172 176 37 773.1 0 37 177 52 773.1 0 52 178 52 773.1 0 52 179 58 773.1 0 58 180 48 773.1 0 48 181 33 773.1 0 33 182 37 773.1 0 37 183 38 773.1 0 38 184 32 773.1 0 32 185 63 773.1 0 63 186 47 773.1 0 47 187 53 773.1 0 53 188 38 773.1 0 38 189 52 773.1 0 52 190 77 773.1 0 77 191 107 773.1 0 107 192 669 773.1 0 669 193 903 773.1 0 903 194 672 773.1 0 672 195 347 773.1 0 347 196 50 773.1 0 50 197 46 773.1 0 46 198 47 773.1 0 47 199 58 773.1 0 58 200 49 773.1 0 49 201 44 773.1 0 44 202 26 773.1 0 26 203 47 773.1 0 47 204 73 773.1 0 73 205 60 773.1 0 60 206 50 773.1 0 50 207 75 773.1 0 75 208 48 773.1 0 48 209 38 773.1 0 38 210 38 773.1 0 38 211 58 773.1 0 58 212 45 773.1 0 45 213 90 773.1 0 90 214 52 773.1 0 52 215 87 773.1 0 87 216 62 773.1 0 62 217 85 773.1 0 85 218 47 773.1 0 47 219 56 773.1 0 56 220 81 773.1 0 81 221 59 773.1 0 59 222 50 773.1 0 50 223 41 773.1 0 41 224 38 773.1 0 38 225 88 773.1 0 88 226 114 773.1 0 114 227 73 773.1 0 73 228 46 773.1 0 46 229 73 773.1 0 73 230 46 773.1 0 46 231 32 773.1 0 32 232 35 773.1 0 35 233 47 773.1 0 47 234 47 773.1 0 47 235 44 773.1 0 44 236 69 773.1 0 69 237 54 773.1 0 54 238 43 773.1 0 43 239 54 773.1 0 54 240 71 773.1 0 71 241 59 773.1 0 59 242 54 773.1 0 54 243 34 773.1 0 34 244 32 773.1 0 32 245 54 773.1 0 54 246 61 773.1 0 61 247 71 773.1 0 71 248 52 773.1 0 52 249 65 773.1 0 65 250 47 773.1 0 47 251 44 773.1 0 44 252 39 773.1 0 39 253 52 773.1 0 52 254 74 773.1 0 74 255 81 773.1 0 81 256 67 773.1 0 67 257 74 773.1 0 74 258 61 773.1 0 61 259 64 773.1 0 64 260 59 773.1 0 59 261 46 773.1 0 46 262 70 773.1 0 70 263 77 773.1 0 77 264 54 773.1 0 54 265 84 773.1 0 84 266 77 773.1 0 77 267 72 773.1 0 72 268 79 773.1 0 79 269 57 773.1 0 57 270 165 773.1 0 165 271 225 773.1 0 225 272 580 773.1 0 580 273 342 773.1 0 342 274 487 773.1 0 487 275 59 773.1 0 59 276 50 773.1 0 50 277 49 773.1 0 49 278 81 773.1 0 81 279 90 773.1 0 90 280 136 773.1 0 136 281 94 773.1 0 94 282 65 773.1 0 65 283 48 773.1 0 48 284 59 773.1 0 59 285 63 773.1 0 63 286 71 773.1 0 71 287 74 773.1 0 74 288 88 773.1 0 88 289 88 773.1 0 88 290 75 773.1 0 75 291 110 773.1 0 110 292 51 773.1 0 51 293 61 773.1 0 61 294 85 773.1 0 85 295 80 773.1 0 80 296 83 773.1 0 83 297 59 773.1 0 59 298 60 773.1 0 60 299 58 773.1 0 58 300 70 773.1 0 70 301 93 773.1 0 93 Finished in 5.35 s (67 us/read; 0.90 M reads/minute). === Summary === Total reads processed: 79,863 Reads with adapters: 30,725 (38.5%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 30,714 (38.5%) Total basepairs processed: 21,532,668 bp Total written (filtered): 8,497,525 bp (39.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30725 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 1 1 16 159 0.0 3 0 156 2 1 17 26899 0.0 3 26358 522 16 3 18 70 0.0 3 2 67 1 19 3533 0.0 3 3446 70 13 4 20 29 0.0 3 2 8 0 19 21 3 0.0 3 0 0 3 26 1 0.0 3 0 1 32 1 0.0 3 1 37 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 1 73 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 1 139 1 0.0 3 1 143 1 0.0 3 1 147 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.69 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 30,714 Reads with adapters: 167 (0.5%) Reads written (passing filters): 30,714 (100.0%) Total basepairs processed: 8,497,525 bp Total written (filtered): 8,497,023 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 167 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.5% C: 9.0% G: 21.0% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 166 479.9 0 166 4 1 120.0 0 1