This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 144.02 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 96,678 (3.1%) Reads that were too short: 7,201 (0.2%) Reads written (passing filters): 89,477 (2.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 22,966,300 bp (2.4%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 96678 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1328 773.1 0 1328 7 25332 773.1 0 25332 8 658 773.1 0 658 9 27350 773.1 0 27350 10 56 773.1 0 56 11 186 773.1 0 186 12 82 773.1 0 82 13 177 773.1 0 177 14 33 773.1 0 33 15 46 773.1 0 46 16 114 773.1 0 114 17 122 773.1 0 122 18 137 773.1 0 137 19 38 773.1 0 38 20 49 773.1 0 49 21 64 773.1 0 64 22 66 773.1 0 66 23 43 773.1 0 43 24 42 773.1 0 42 25 41 773.1 0 41 26 37 773.1 0 37 27 28 773.1 0 28 28 35 773.1 0 35 29 52 773.1 0 52 30 33 773.1 0 33 31 45 773.1 0 45 32 68 773.1 0 68 33 65 773.1 0 65 34 46 773.1 0 46 35 41 773.1 0 41 36 48 773.1 0 48 37 52 773.1 0 52 38 46 773.1 0 46 39 50 773.1 0 50 40 33 773.1 0 33 41 38 773.1 0 38 42 68 773.1 0 68 43 58 773.1 0 58 44 55 773.1 0 55 45 54 773.1 0 54 46 427 773.1 0 427 47 498 773.1 0 498 48 354 773.1 0 354 49 58 773.1 0 58 50 61 773.1 0 61 51 62 773.1 0 62 52 50 773.1 0 50 53 41 773.1 0 41 54 32 773.1 0 32 55 39 773.1 0 39 56 62 773.1 0 62 57 51 773.1 0 51 58 34 773.1 0 34 59 63 773.1 0 63 60 43 773.1 0 43 61 66 773.1 0 66 62 41 773.1 0 41 63 82 773.1 0 82 64 48 773.1 0 48 65 68 773.1 0 68 66 70 773.1 0 70 67 68 773.1 0 68 68 42 773.1 0 42 69 55 773.1 0 55 70 61 773.1 0 61 71 54 773.1 0 54 72 52 773.1 0 52 73 46 773.1 0 46 74 58 773.1 0 58 75 51 773.1 0 51 76 85 773.1 0 85 77 60 773.1 0 60 78 67 773.1 0 67 79 313 773.1 0 313 80 325 773.1 0 325 81 560 773.1 0 560 82 105 773.1 0 105 83 150 773.1 0 150 84 118 773.1 0 118 85 101 773.1 0 101 86 81 773.1 0 81 87 74 773.1 0 74 88 80 773.1 0 80 89 74 773.1 0 74 90 97 773.1 0 97 91 121 773.1 0 121 92 183 773.1 0 183 93 249 773.1 0 249 94 3166 773.1 0 3166 95 2508 773.1 0 2508 96 6635 773.1 0 6635 97 1793 773.1 0 1793 98 2734 773.1 0 2734 99 45 773.1 0 45 100 41 773.1 0 41 101 37 773.1 0 37 102 42 773.1 0 42 103 28 773.1 0 28 104 43 773.1 0 43 105 57 773.1 0 57 106 34 773.1 0 34 107 47 773.1 0 47 108 90 773.1 0 90 109 44 773.1 0 44 110 120 773.1 0 120 111 80 773.1 0 80 112 78 773.1 0 78 113 44 773.1 0 44 114 46 773.1 0 46 115 46 773.1 0 46 116 60 773.1 0 60 117 72 773.1 0 72 118 203 773.1 0 203 119 46 773.1 0 46 120 60 773.1 0 60 121 56 773.1 0 56 122 64 773.1 0 64 123 64 773.1 0 64 124 420 773.1 0 420 125 245 773.1 0 245 126 221 773.1 0 221 127 62 773.1 0 62 128 45 773.1 0 45 129 91 773.1 0 91 130 219 773.1 0 219 131 466 773.1 0 466 132 307 773.1 0 307 133 501 773.1 0 501 134 148 773.1 0 148 135 68 773.1 0 68 136 78 773.1 0 78 137 162 773.1 0 162 138 249 773.1 0 249 139 545 773.1 0 545 140 71 773.1 0 71 141 118 773.1 0 118 142 108 773.1 0 108 143 169 773.1 0 169 144 151 773.1 0 151 145 133 773.1 0 133 146 124 773.1 0 124 147 93 773.1 0 93 148 84 773.1 0 84 149 119 773.1 0 119 150 59 773.1 0 59 151 56 773.1 0 56 152 40 773.1 0 40 153 41 773.1 0 41 154 41 773.1 0 41 155 47 773.1 0 47 156 59 773.1 0 59 157 78 773.1 0 78 158 364 773.1 0 364 159 219 773.1 0 219 160 308 773.1 0 308 161 41 773.1 0 41 162 38 773.1 0 38 163 49 773.1 0 49 164 46 773.1 0 46 165 58 773.1 0 58 166 49 773.1 0 49 167 40 773.1 0 40 168 59 773.1 0 59 169 47 773.1 0 47 170 38 773.1 0 38 171 38 773.1 0 38 172 39 773.1 0 39 173 43 773.1 0 43 174 50 773.1 0 50 175 40 773.1 0 40 176 88 773.1 0 88 177 67 773.1 0 67 178 54 773.1 0 54 179 62 773.1 0 62 180 52 773.1 0 52 181 48 773.1 0 48 182 52 773.1 0 52 183 59 773.1 0 59 184 80 773.1 0 80 185 47 773.1 0 47 186 110 773.1 0 110 187 71 773.1 0 71 188 85 773.1 0 85 189 45 773.1 0 45 190 49 773.1 0 49 191 42 773.1 0 42 192 126 773.1 0 126 193 86 773.1 0 86 194 101 773.1 0 101 195 43 773.1 0 43 196 52 773.1 0 52 197 45 773.1 0 45 198 40 773.1 0 40 199 47 773.1 0 47 200 32 773.1 0 32 201 31 773.1 0 31 202 38 773.1 0 38 203 59 773.1 0 59 204 56 773.1 0 56 205 335 773.1 0 335 206 422 773.1 0 422 207 530 773.1 0 530 208 47 773.1 0 47 209 45 773.1 0 45 210 49 773.1 0 49 211 33 773.1 0 33 212 31 773.1 0 31 213 42 773.1 0 42 214 38 773.1 0 38 215 25 773.1 0 25 216 31 773.1 0 31 217 41 773.1 0 41 218 26 773.1 0 26 219 43 773.1 0 43 220 34 773.1 0 34 221 36 773.1 0 36 222 42 773.1 0 42 223 70 773.1 0 70 224 47 773.1 0 47 225 26 773.1 0 26 226 42 773.1 0 42 227 61 773.1 0 61 228 63 773.1 0 63 229 55 773.1 0 55 230 51 773.1 0 51 231 29 773.1 0 29 232 60 773.1 0 60 233 62 773.1 0 62 234 36 773.1 0 36 235 37 773.1 0 37 236 45 773.1 0 45 237 48 773.1 0 48 238 51 773.1 0 51 239 53 773.1 0 53 240 54 773.1 0 54 241 63 773.1 0 63 242 48 773.1 0 48 243 50 773.1 0 50 244 43 773.1 0 43 245 48 773.1 0 48 246 44 773.1 0 44 247 50 773.1 0 50 248 41 773.1 0 41 249 51 773.1 0 51 250 44 773.1 0 44 251 37 773.1 0 37 252 70 773.1 0 70 253 68 773.1 0 68 254 107 773.1 0 107 255 56 773.1 0 56 256 56 773.1 0 56 257 76 773.1 0 76 258 45 773.1 0 45 259 52 773.1 0 52 260 39 773.1 0 39 261 76 773.1 0 76 262 81 773.1 0 81 263 86 773.1 0 86 264 77 773.1 0 77 265 37 773.1 0 37 266 78 773.1 0 78 267 66 773.1 0 66 268 37 773.1 0 37 269 47 773.1 0 47 270 89 773.1 0 89 271 107 773.1 0 107 272 112 773.1 0 112 273 64 773.1 0 64 274 56 773.1 0 56 275 47 773.1 0 47 276 73 773.1 0 73 277 94 773.1 0 94 278 87 773.1 0 87 279 77 773.1 0 77 280 44 773.1 0 44 281 94 773.1 0 94 282 144 773.1 0 144 283 148 773.1 0 148 284 78 773.1 0 78 285 42 773.1 0 42 286 65 773.1 0 65 287 82 773.1 0 82 288 102 773.1 0 102 289 101 773.1 0 101 290 99 773.1 0 99 291 53 773.1 0 53 292 72 773.1 0 72 293 41 773.1 0 41 294 54 773.1 0 54 295 83 773.1 0 83 296 72 773.1 0 72 297 61 773.1 0 61 298 76 773.1 0 76 299 118 773.1 0 118 300 127 773.1 0 127 301 73 773.1 0 73 Finished in 6.34 s (71 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 89,477 Reads with adapters: 26,806 (30.0%) Reads that were too short: 96 (0.1%) Reads written (passing filters): 26,710 (29.9%) Total basepairs processed: 22,966,300 bp Total written (filtered): 7,146,234 bp (31.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26806 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 5 19 262 0.0 3 0 231 14 15 2 20 24740 0.0 4 23679 884 129 42 6 21 101 0.0 4 25 64 7 2 3 22 6 0.0 4 1 0 4 1 23 37 0.0 4 0 0 0 26 11 24 4 0.0 4 3 0 0 1 27 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 56 1 0.0 4 1 74 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 116 3 0.0 4 0 0 0 0 3 117 5 0.0 4 0 0 0 0 5 124 1 0.0 4 0 0 0 0 1 126 19 0.0 4 0 0 0 0 19 127 1089 0.0 4 0 0 0 0 1089 128 1 0.0 4 0 0 0 0 1 130 47 0.0 4 0 0 0 0 47 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 134 4 0.0 4 0 0 0 0 4 135 378 0.0 4 0 0 0 0 378 138 1 0.0 4 0 0 0 0 1 141 48 0.0 4 0 0 0 2 46 142 18 0.0 4 0 0 0 0 18 144 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 1 258 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.36 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 26,710 Reads with adapters: 1,168 (4.4%) Reads written (passing filters): 26,710 (100.0%) Total basepairs processed: 7,146,234 bp Total written (filtered): 7,131,723 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1168 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.3% C: 12.0% G: 78.1% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 111 417.3 0 111 4 16 104.3 0 16 9 16 0.1 0 1 15 10 19 0.0 1 1 18 11 672 0.0 1 16 656 12 308 0.0 1 3 305 13 5 0.0 1 0 5 14 2 0.0 1 0 2 15 4 0.0 1 0 4 152 11 0.0 1 6 5 210 2 0.0 1 2 222 1 0.0 1 0 1 225 1 0.0 1 1