This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 146.41 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 288,525 (9.1%) Reads that were too short: 14,987 (0.5%) Reads written (passing filters): 273,538 (8.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 50,184,484 bp (5.3%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 288525 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 141 773.1 0 141 7 4038 773.1 0 4038 8 148 773.1 0 148 9 4282 773.1 0 4282 10 18 773.1 0 18 11 18 773.1 0 18 12 19 773.1 0 19 13 33 773.1 0 33 14 19 773.1 0 19 15 35 773.1 0 35 16 33 773.1 0 33 17 28 773.1 0 28 18 17 773.1 0 17 19 29 773.1 0 29 20 29 773.1 0 29 21 45 773.1 0 45 22 26 773.1 0 26 23 36 773.1 0 36 24 30 773.1 0 30 25 38 773.1 0 38 26 42 773.1 0 42 27 44 773.1 0 44 28 30 773.1 0 30 29 22 773.1 0 22 30 31 773.1 0 31 31 44 773.1 0 44 32 42 773.1 0 42 33 53 773.1 0 53 34 34 773.1 0 34 35 42 773.1 0 42 36 30 773.1 0 30 37 49 773.1 0 49 38 34 773.1 0 34 39 76 773.1 0 76 40 46 773.1 0 46 41 31 773.1 0 31 42 34 773.1 0 34 43 30 773.1 0 30 44 29 773.1 0 29 45 38 773.1 0 38 46 48 773.1 0 48 47 40 773.1 0 40 48 43 773.1 0 43 49 36 773.1 0 36 50 32 773.1 0 32 51 56 773.1 0 56 52 77 773.1 0 77 53 47 773.1 0 47 54 62 773.1 0 62 55 65 773.1 0 65 56 117 773.1 0 117 57 93 773.1 0 93 58 105 773.1 0 105 59 1146 773.1 0 1146 60 227 773.1 0 227 61 236 773.1 0 236 62 52 773.1 0 52 63 28 773.1 0 28 64 53 773.1 0 53 65 48 773.1 0 48 66 25 773.1 0 25 67 52 773.1 0 52 68 50 773.1 0 50 69 80 773.1 0 80 70 76 773.1 0 76 71 43 773.1 0 43 72 64 773.1 0 64 73 38 773.1 0 38 74 68 773.1 0 68 75 61 773.1 0 61 76 61 773.1 0 61 77 270 773.1 0 270 78 58 773.1 0 58 79 49 773.1 0 49 80 47 773.1 0 47 81 51 773.1 0 51 82 43 773.1 0 43 83 63 773.1 0 63 84 81 773.1 0 81 85 67 773.1 0 67 86 83 773.1 0 83 87 647 773.1 0 647 88 58 773.1 0 58 89 64 773.1 0 64 90 62 773.1 0 62 91 53 773.1 0 53 92 107 773.1 0 107 93 59 773.1 0 59 94 82 773.1 0 82 95 105 773.1 0 105 96 157 773.1 0 157 97 340 773.1 0 340 98 104 773.1 0 104 99 168 773.1 0 168 100 160 773.1 0 160 101 205 773.1 0 205 102 643 773.1 0 643 103 252 773.1 0 252 104 127 773.1 0 127 105 78 773.1 0 78 106 295 773.1 0 295 107 376 773.1 0 376 108 412 773.1 0 412 109 466 773.1 0 466 110 703 773.1 0 703 111 725 773.1 0 725 112 1723 773.1 0 1723 113 3400 773.1 0 3400 114 61884 773.1 0 61884 115 37977 773.1 0 37977 116 93208 773.1 0 93208 117 479 773.1 0 479 118 81 773.1 0 81 119 41 773.1 0 41 120 60 773.1 0 60 121 36 773.1 0 36 122 19 773.1 0 19 123 45 773.1 0 45 124 68 773.1 0 68 125 47 773.1 0 47 126 91 773.1 0 91 127 88 773.1 0 88 128 184 773.1 0 184 129 148 773.1 0 148 130 86 773.1 0 86 131 99 773.1 0 99 132 336 773.1 0 336 133 45 773.1 0 45 134 51 773.1 0 51 135 83 773.1 0 83 136 80 773.1 0 80 137 131 773.1 0 131 138 70 773.1 0 70 139 55 773.1 0 55 140 181 773.1 0 181 141 335 773.1 0 335 142 306 773.1 0 306 143 275 773.1 0 275 144 185 773.1 0 185 145 294 773.1 0 294 146 445 773.1 0 445 147 12640 773.1 0 12640 148 5164 773.1 0 5164 149 11399 773.1 0 11399 150 259 773.1 0 259 151 183 773.1 0 183 152 389 773.1 0 389 153 6144 773.1 0 6144 154 4089 773.1 0 4089 155 5765 773.1 0 5765 156 270 773.1 0 270 157 246 773.1 0 246 158 94 773.1 0 94 159 36 773.1 0 36 160 56 773.1 0 56 161 56 773.1 0 56 162 123 773.1 0 123 163 63 773.1 0 63 164 48 773.1 0 48 165 46 773.1 0 46 166 41 773.1 0 41 167 53 773.1 0 53 168 185 773.1 0 185 169 206 773.1 0 206 170 136 773.1 0 136 171 74 773.1 0 74 172 46 773.1 0 46 173 30 773.1 0 30 174 32 773.1 0 32 175 64 773.1 0 64 176 47 773.1 0 47 177 45 773.1 0 45 178 31 773.1 0 31 179 36 773.1 0 36 180 38 773.1 0 38 181 92 773.1 0 92 182 106 773.1 0 106 183 277 773.1 0 277 184 168 773.1 0 168 185 269 773.1 0 269 186 203 773.1 0 203 187 63 773.1 0 63 188 57 773.1 0 57 189 125 773.1 0 125 190 125 773.1 0 125 191 417 773.1 0 417 192 39 773.1 0 39 193 91 773.1 0 91 194 32 773.1 0 32 195 48 773.1 0 48 196 26 773.1 0 26 197 53 773.1 0 53 198 108 773.1 0 108 199 56 773.1 0 56 200 36 773.1 0 36 201 45 773.1 0 45 202 187 773.1 0 187 203 116 773.1 0 116 204 155 773.1 0 155 205 44 773.1 0 44 206 29 773.1 0 29 207 41 773.1 0 41 208 39 773.1 0 39 209 32 773.1 0 32 210 27 773.1 0 27 211 37 773.1 0 37 212 26 773.1 0 26 213 33 773.1 0 33 214 31 773.1 0 31 215 33 773.1 0 33 216 175 773.1 0 175 217 122 773.1 0 122 218 158 773.1 0 158 219 28 773.1 0 28 220 35 773.1 0 35 221 46 773.1 0 46 222 37 773.1 0 37 223 25 773.1 0 25 224 26 773.1 0 26 225 26 773.1 0 26 226 72 773.1 0 72 227 41 773.1 0 41 228 58 773.1 0 58 229 31 773.1 0 31 230 36 773.1 0 36 231 35 773.1 0 35 232 41 773.1 0 41 233 59 773.1 0 59 234 92 773.1 0 92 235 125 773.1 0 125 236 101 773.1 0 101 237 55 773.1 0 55 238 55 773.1 0 55 239 42 773.1 0 42 240 52 773.1 0 52 241 26 773.1 0 26 242 47 773.1 0 47 243 30 773.1 0 30 244 25 773.1 0 25 245 41 773.1 0 41 246 75 773.1 0 75 247 34 773.1 0 34 248 41 773.1 0 41 249 65 773.1 0 65 250 54 773.1 0 54 251 49 773.1 0 49 252 36 773.1 0 36 253 46 773.1 0 46 254 42 773.1 0 42 255 36 773.1 0 36 256 27 773.1 0 27 257 38 773.1 0 38 258 38 773.1 0 38 259 47 773.1 0 47 260 35 773.1 0 35 261 37 773.1 0 37 262 80 773.1 0 80 263 45 773.1 0 45 264 29 773.1 0 29 265 53 773.1 0 53 266 53 773.1 0 53 267 94 773.1 0 94 268 94 773.1 0 94 269 64 773.1 0 64 270 103 773.1 0 103 271 104 773.1 0 104 272 122 773.1 0 122 273 81 773.1 0 81 274 81 773.1 0 81 275 112 773.1 0 112 276 82 773.1 0 82 277 90 773.1 0 90 278 166 773.1 0 166 279 403 773.1 0 403 280 746 773.1 0 746 281 2583 773.1 0 2583 282 1766 773.1 0 1766 283 2610 773.1 0 2610 284 112 773.1 0 112 285 110 773.1 0 110 286 100 773.1 0 100 287 46 773.1 0 46 288 34 773.1 0 34 289 87 773.1 0 87 290 276 773.1 0 276 291 317 773.1 0 317 292 294 773.1 0 294 293 176 773.1 0 176 294 123 773.1 0 123 295 104 773.1 0 104 296 96 773.1 0 96 297 100 773.1 0 100 298 55 773.1 0 55 299 42 773.1 0 42 300 41 773.1 0 41 301 41 773.1 0 41 Finished in 14.43 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 273,538 Reads with adapters: 4,057 (1.5%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 4,014 (1.5%) Total basepairs processed: 50,184,484 bp Total written (filtered): 1,103,854 bp (2.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4057 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 36 0.0 3 0 34 2 17 3909 0.0 3 3823 83 3 18 14 0.0 3 3 10 1 19 2 0.0 3 0 0 2 20 1 0.0 3 1 24 1 0.0 3 0 0 0 1 33 7 0.0 3 0 0 0 7 42 1 0.0 3 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 6 0.0 3 0 0 0 6 53 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 74 1 0.0 3 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 85 23 0.0 3 0 0 0 23 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 1 110 6 0.0 3 0 0 0 6 129 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 138 2 0.0 3 1 0 0 1 139 1 0.0 3 1 148 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 172 6 0.0 3 0 0 0 6 173 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 175 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 4,014 Reads with adapters: 6 (0.1%) Reads written (passing filters): 4,014 (100.0%) Total basepairs processed: 1,103,854 bp Total written (filtered): 1,103,836 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 50.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 62.7 0 6