This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 138.14 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 114,002 (3.6%) Reads that were too short: 80,941 (2.6%) Reads written (passing filters): 33,061 (1.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 8,684,707 bp (0.9%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 114002 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 472 773.1 0 472 7 12083 773.1 0 12083 8 261 773.1 0 261 9 9987 773.1 0 9987 10 40 773.1 0 40 11 37 773.1 0 37 12 38 773.1 0 38 13 47 773.1 0 47 14 36 773.1 0 36 15 47 773.1 0 47 16 52 773.1 0 52 17 56 773.1 0 56 18 63 773.1 0 63 19 45 773.1 0 45 20 43 773.1 0 43 21 43 773.1 0 43 22 46 773.1 0 46 23 49 773.1 0 49 24 36 773.1 0 36 25 40 773.1 0 40 26 43 773.1 0 43 27 28 773.1 0 28 28 61 773.1 0 61 29 51 773.1 0 51 30 42 773.1 0 42 31 36 773.1 0 36 32 42 773.1 0 42 33 71 773.1 0 71 34 67 773.1 0 67 35 52 773.1 0 52 36 39 773.1 0 39 37 52 773.1 0 52 38 63 773.1 0 63 39 54 773.1 0 54 40 54 773.1 0 54 41 42 773.1 0 42 42 55 773.1 0 55 43 45 773.1 0 45 44 55 773.1 0 55 45 38 773.1 0 38 46 38 773.1 0 38 47 60 773.1 0 60 48 39 773.1 0 39 49 55 773.1 0 55 50 41 773.1 0 41 51 54 773.1 0 54 52 61 773.1 0 61 53 62 773.1 0 62 54 123 773.1 0 123 55 51 773.1 0 51 56 38 773.1 0 38 57 53 773.1 0 53 58 73 773.1 0 73 59 55 773.1 0 55 60 68 773.1 0 68 61 39 773.1 0 39 62 44 773.1 0 44 63 65 773.1 0 65 64 50 773.1 0 50 65 40 773.1 0 40 66 38 773.1 0 38 67 39 773.1 0 39 68 38 773.1 0 38 69 44 773.1 0 44 70 44 773.1 0 44 71 116 773.1 0 116 72 118 773.1 0 118 73 106 773.1 0 106 74 82 773.1 0 82 75 32 773.1 0 32 76 21 773.1 0 21 77 34 773.1 0 34 78 23 773.1 0 23 79 35 773.1 0 35 80 35 773.1 0 35 81 37 773.1 0 37 82 33 773.1 0 33 83 35 773.1 0 35 84 36 773.1 0 36 85 44 773.1 0 44 86 29 773.1 0 29 87 22 773.1 0 22 88 38 773.1 0 38 89 35 773.1 0 35 90 31 773.1 0 31 91 49 773.1 0 49 92 69 773.1 0 69 93 32 773.1 0 32 94 33 773.1 0 33 95 44 773.1 0 44 96 56 773.1 0 56 97 79 773.1 0 79 98 28 773.1 0 28 99 39 773.1 0 39 100 39 773.1 0 39 101 37 773.1 0 37 102 39 773.1 0 39 103 55 773.1 0 55 104 40 773.1 0 40 105 35 773.1 0 35 106 29 773.1 0 29 107 25 773.1 0 25 108 28 773.1 0 28 109 27 773.1 0 27 110 35 773.1 0 35 111 42 773.1 0 42 112 23 773.1 0 23 113 33 773.1 0 33 114 25 773.1 0 25 115 33 773.1 0 33 116 27 773.1 0 27 117 45 773.1 0 45 118 33 773.1 0 33 119 121 773.1 0 121 120 71 773.1 0 71 121 32 773.1 0 32 122 20 773.1 0 20 123 84 773.1 0 84 124 345 773.1 0 345 125 32 773.1 0 32 126 34 773.1 0 34 127 70 773.1 0 70 128 38 773.1 0 38 129 44 773.1 0 44 130 116 773.1 0 116 131 353 773.1 0 353 132 188 773.1 0 188 133 150 773.1 0 150 134 146 773.1 0 146 135 30 773.1 0 30 136 93 773.1 0 93 137 359 773.1 0 359 138 258 773.1 0 258 139 193 773.1 0 193 140 80 773.1 0 80 141 32 773.1 0 32 142 40 773.1 0 40 143 26 773.1 0 26 144 27 773.1 0 27 145 33 773.1 0 33 146 51 773.1 0 51 147 19 773.1 0 19 148 41 773.1 0 41 149 33 773.1 0 33 150 39 773.1 0 39 151 88 773.1 0 88 152 67 773.1 0 67 153 63 773.1 0 63 154 21 773.1 0 21 155 30 773.1 0 30 156 40 773.1 0 40 157 36 773.1 0 36 158 39 773.1 0 39 159 29 773.1 0 29 160 21 773.1 0 21 161 19 773.1 0 19 162 26 773.1 0 26 163 33 773.1 0 33 164 29 773.1 0 29 165 21 773.1 0 21 166 31 773.1 0 31 167 32 773.1 0 32 168 39 773.1 0 39 169 18 773.1 0 18 170 29 773.1 0 29 171 22 773.1 0 22 172 26 773.1 0 26 173 30 773.1 0 30 174 35 773.1 0 35 175 23 773.1 0 23 176 32 773.1 0 32 177 50 773.1 0 50 178 261 773.1 0 261 179 48 773.1 0 48 180 21 773.1 0 21 181 22 773.1 0 22 182 23 773.1 0 23 183 38 773.1 0 38 184 34 773.1 0 34 185 37 773.1 0 37 186 78 773.1 0 78 187 74 773.1 0 74 188 70 773.1 0 70 189 36 773.1 0 36 190 42 773.1 0 42 191 25 773.1 0 25 192 28 773.1 0 28 193 27 773.1 0 27 194 23 773.1 0 23 195 29 773.1 0 29 196 23 773.1 0 23 197 48 773.1 0 48 198 27 773.1 0 27 199 30 773.1 0 30 200 20 773.1 0 20 201 26 773.1 0 26 202 21 773.1 0 21 203 26 773.1 0 26 204 43 773.1 0 43 205 47 773.1 0 47 206 32 773.1 0 32 207 16 773.1 0 16 208 25 773.1 0 25 209 22 773.1 0 22 210 25 773.1 0 25 211 40 773.1 0 40 212 212 773.1 0 212 213 258 773.1 0 258 214 141 773.1 0 141 215 23 773.1 0 23 216 42 773.1 0 42 217 62 773.1 0 62 218 193 773.1 0 193 219 112 773.1 0 112 220 128 773.1 0 128 221 42 773.1 0 42 222 35 773.1 0 35 223 19 773.1 0 19 224 28 773.1 0 28 225 32 773.1 0 32 226 33 773.1 0 33 227 28 773.1 0 28 228 41 773.1 0 41 229 34 773.1 0 34 230 33 773.1 0 33 231 39 773.1 0 39 232 62 773.1 0 62 233 38 773.1 0 38 234 40 773.1 0 40 235 110 773.1 0 110 236 590 773.1 0 590 237 173 773.1 0 173 238 312 773.1 0 312 239 47 773.1 0 47 240 35 773.1 0 35 241 71 773.1 0 71 242 59 773.1 0 59 243 252 773.1 0 252 244 420 773.1 0 420 245 486 773.1 0 486 246 176 773.1 0 176 247 74 773.1 0 74 248 47 773.1 0 47 249 50 773.1 0 50 250 61 773.1 0 61 251 58 773.1 0 58 252 56 773.1 0 56 253 58 773.1 0 58 254 63 773.1 0 63 255 197 773.1 0 197 256 274 773.1 0 274 257 319 773.1 0 319 258 235 773.1 0 235 259 139 773.1 0 139 260 1380 773.1 0 1380 261 691 773.1 0 691 262 1057 773.1 0 1057 263 325 773.1 0 325 264 44 773.1 0 44 265 101 773.1 0 101 266 139 773.1 0 139 267 147 773.1 0 147 268 88 773.1 0 88 269 84 773.1 0 84 270 106 773.1 0 106 271 123 773.1 0 123 272 188 773.1 0 188 273 293 773.1 0 293 274 166 773.1 0 166 275 196 773.1 0 196 276 422 773.1 0 422 277 164 773.1 0 164 278 129 773.1 0 129 279 455 773.1 0 455 280 502 773.1 0 502 281 795 773.1 0 795 282 566 773.1 0 566 283 464 773.1 0 464 284 514 773.1 0 514 285 773 773.1 0 773 286 19935 773.1 0 19935 287 17647 773.1 0 17647 288 25506 773.1 0 25506 289 123 773.1 0 123 290 54 773.1 0 54 291 66 773.1 0 66 292 90 773.1 0 90 293 58 773.1 0 58 294 38 773.1 0 38 295 76 773.1 0 76 296 289 773.1 0 289 297 189 773.1 0 189 298 281 773.1 0 281 299 207 773.1 0 207 300 112 773.1 0 112 301 24 773.1 0 24 Finished in 2.23 s (67 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 33,061 Reads with adapters: 10,325 (31.2%) Reads that were too short: 22 (0.1%) Reads written (passing filters): 10,303 (31.2%) Total basepairs processed: 8,684,707 bp Total written (filtered): 2,831,683 bp (32.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10325 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 62 0.0 3 0 58 2 2 17 10186 0.0 3 9958 223 5 18 18 0.0 3 1 17 19 15 0.0 3 8 0 5 2 20 8 0.0 3 0 0 0 8 23 1 0.0 3 1 25 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 1 0 0 1 66 2 0.0 3 1 0 1 72 1 0.0 3 0 0 0 1 88 1 0.0 3 1 98 1 0.0 3 0 0 0 1 107 3 0.0 3 0 0 0 3 114 1 0.0 3 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 170 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 10,303 Reads with adapters: 430 (4.2%) Reads written (passing filters): 10,303 (100.0%) Total basepairs processed: 2,831,683 bp Total written (filtered): 2,830,377 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 430 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.7% C: 6.5% G: 15.8% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 414 161.0 0 414 4 16 40.2 0 16