This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.51 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 79,060 (3.1%) Reads that were too short: 28,730 (1.1%) Reads written (passing filters): 50,330 (2.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,266,789 bp (1.5%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 79060 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 628.5 0 208 7 640 628.5 0 640 8 9610 628.5 0 9610 9 8796 628.5 0 8796 10 97 628.5 0 97 11 76 628.5 0 76 12 88 628.5 0 88 13 81 628.5 0 81 14 83 628.5 0 83 15 75 628.5 0 75 16 79 628.5 0 79 17 88 628.5 0 88 18 53 628.5 0 53 19 54 628.5 0 54 20 106 628.5 0 106 21 90 628.5 0 90 22 105 628.5 0 105 23 80 628.5 0 80 24 93 628.5 0 93 25 87 628.5 0 87 26 63 628.5 0 63 27 92 628.5 0 92 28 82 628.5 0 82 29 79 628.5 0 79 30 87 628.5 0 87 31 93 628.5 0 93 32 97 628.5 0 97 33 90 628.5 0 90 34 79 628.5 0 79 35 87 628.5 0 87 36 97 628.5 0 97 37 90 628.5 0 90 38 171 628.5 0 171 39 104 628.5 0 104 40 193 628.5 0 193 41 91 628.5 0 91 42 86 628.5 0 86 43 69 628.5 0 69 44 65 628.5 0 65 45 68 628.5 0 68 46 86 628.5 0 86 47 76 628.5 0 76 48 71 628.5 0 71 49 82 628.5 0 82 50 75 628.5 0 75 51 104 628.5 0 104 52 113 628.5 0 113 53 88 628.5 0 88 54 90 628.5 0 90 55 111 628.5 0 111 56 96 628.5 0 96 57 78 628.5 0 78 58 94 628.5 0 94 59 84 628.5 0 84 60 127 628.5 0 127 61 102 628.5 0 102 62 86 628.5 0 86 63 117 628.5 0 117 64 108 628.5 0 108 65 93 628.5 0 93 66 112 628.5 0 112 67 121 628.5 0 121 68 147 628.5 0 147 69 122 628.5 0 122 70 99 628.5 0 99 71 99 628.5 0 99 72 106 628.5 0 106 73 98 628.5 0 98 74 175 628.5 0 175 75 145 628.5 0 145 76 104 628.5 0 104 77 88 628.5 0 88 78 140 628.5 0 140 79 113 628.5 0 113 80 108 628.5 0 108 81 91 628.5 0 91 82 134 628.5 0 134 83 87 628.5 0 87 84 102 628.5 0 102 85 70 628.5 0 70 86 95 628.5 0 95 87 123 628.5 0 123 88 114 628.5 0 114 89 130 628.5 0 130 90 86 628.5 0 86 91 150 628.5 0 150 92 112 628.5 0 112 93 110 628.5 0 110 94 168 628.5 0 168 95 203 628.5 0 203 96 207 628.5 0 207 97 1513 628.5 0 1513 98 805 628.5 0 805 99 1233 628.5 0 1233 100 321 628.5 0 321 101 104 628.5 0 104 102 64 628.5 0 64 103 82 628.5 0 82 104 95 628.5 0 95 105 105 628.5 0 105 106 108 628.5 0 108 107 143 628.5 0 143 108 190 628.5 0 190 109 231 628.5 0 231 110 151 628.5 0 151 111 636 628.5 0 636 112 666 628.5 0 666 113 736 628.5 0 736 114 100 628.5 0 100 115 81 628.5 0 81 116 80 628.5 0 80 117 110 628.5 0 110 118 96 628.5 0 96 119 99 628.5 0 99 120 95 628.5 0 95 121 80 628.5 0 80 122 100 628.5 0 100 123 72 628.5 0 72 124 88 628.5 0 88 125 99 628.5 0 99 126 114 628.5 0 114 127 83 628.5 0 83 128 121 628.5 0 121 129 133 628.5 0 133 130 164 628.5 0 164 131 131 628.5 0 131 132 173 628.5 0 173 133 158 628.5 0 158 134 314 628.5 0 314 135 119 628.5 0 119 136 135 628.5 0 135 137 100 628.5 0 100 138 90 628.5 0 90 139 88 628.5 0 88 140 112 628.5 0 112 141 119 628.5 0 119 142 124 628.5 0 124 143 104 628.5 0 104 144 106 628.5 0 106 145 106 628.5 0 106 146 143 628.5 0 143 147 180 628.5 0 180 148 168 628.5 0 168 149 124 628.5 0 124 150 86 628.5 0 86 151 112 628.5 0 112 152 117 628.5 0 117 153 123 628.5 0 123 154 243 628.5 0 243 155 390 628.5 0 390 156 799 628.5 0 799 157 842 628.5 0 842 158 91 628.5 0 91 159 98 628.5 0 98 160 112 628.5 0 112 161 106 628.5 0 106 162 98 628.5 0 98 163 75 628.5 0 75 164 117 628.5 0 117 165 102 628.5 0 102 166 315 628.5 0 315 167 232 628.5 0 232 168 327 628.5 0 327 169 90 628.5 0 90 170 103 628.5 0 103 171 94 628.5 0 94 172 101 628.5 0 101 173 67 628.5 0 67 174 92 628.5 0 92 175 96 628.5 0 96 176 91 628.5 0 91 177 87 628.5 0 87 178 105 628.5 0 105 179 102 628.5 0 102 180 127 628.5 0 127 181 129 628.5 0 129 182 101 628.5 0 101 183 105 628.5 0 105 184 68 628.5 0 68 185 115 628.5 0 115 186 126 628.5 0 126 187 107 628.5 0 107 188 121 628.5 0 121 189 115 628.5 0 115 190 139 628.5 0 139 191 97 628.5 0 97 192 151 628.5 0 151 193 465 628.5 0 465 194 864 628.5 0 864 195 914 628.5 0 914 196 443 628.5 0 443 197 148 628.5 0 148 198 150 628.5 0 150 199 155 628.5 0 155 200 340 628.5 0 340 201 333 628.5 0 333 202 383 628.5 0 383 203 118 628.5 0 118 204 101 628.5 0 101 205 100 628.5 0 100 206 118 628.5 0 118 207 153 628.5 0 153 208 120 628.5 0 120 209 101 628.5 0 101 210 116 628.5 0 116 211 108 628.5 0 108 212 98 628.5 0 98 213 106 628.5 0 106 214 117 628.5 0 117 215 155 628.5 0 155 216 187 628.5 0 187 217 186 628.5 0 186 218 142 628.5 0 142 219 121 628.5 0 121 220 626 628.5 0 626 221 740 628.5 0 740 222 805 628.5 0 805 223 897 628.5 0 897 224 99 628.5 0 99 225 90 628.5 0 90 226 97 628.5 0 97 227 114 628.5 0 114 228 122 628.5 0 122 229 129 628.5 0 129 230 100 628.5 0 100 231 80 628.5 0 80 232 93 628.5 0 93 233 129 628.5 0 129 234 84 628.5 0 84 235 97 628.5 0 97 236 127 628.5 0 127 237 142 628.5 0 142 238 198 628.5 0 198 239 458 628.5 0 458 240 187 628.5 0 187 241 281 628.5 0 281 242 247 628.5 0 247 243 720 628.5 0 720 244 377 628.5 0 377 245 538 628.5 0 538 246 278 628.5 0 278 247 94 628.5 0 94 248 134 628.5 0 134 249 141 628.5 0 141 250 165 628.5 0 165 251 211 628.5 0 211 252 233 628.5 0 233 253 240 628.5 0 240 254 510 628.5 0 510 255 555 628.5 0 555 256 978 628.5 0 978 257 155 628.5 0 155 258 174 628.5 0 174 259 165 628.5 0 165 260 179 628.5 0 179 261 212 628.5 0 212 262 1615 628.5 0 1615 263 1893 628.5 0 1893 264 3633 628.5 0 3633 265 252 628.5 0 252 266 111 628.5 0 111 267 84 628.5 0 84 268 110 628.5 0 110 269 157 628.5 0 157 270 167 628.5 0 167 271 183 628.5 0 183 272 169 628.5 0 169 273 205 628.5 0 205 274 127 628.5 0 127 275 157 628.5 0 157 276 175 628.5 0 175 277 128 628.5 0 128 278 102 628.5 0 102 279 147 628.5 0 147 280 337 628.5 0 337 281 152 628.5 0 152 282 275 628.5 0 275 283 171 628.5 0 171 284 128 628.5 0 128 285 130 628.5 0 130 286 135 628.5 0 135 287 112 628.5 0 112 288 130 628.5 0 130 289 214 628.5 0 214 290 194 628.5 0 194 291 220 628.5 0 220 292 145 628.5 0 145 293 603 628.5 0 603 294 643 628.5 0 643 295 222 628.5 0 222 296 143 628.5 0 143 297 136 628.5 0 136 298 147 628.5 0 147 299 167 628.5 0 167 300 212 628.5 0 212 301 168 628.5 0 168 Finished in 2.41 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 50,330 Reads with adapters: 9,764 (19.4%) Reads that were too short: 26 (0.1%) Reads written (passing filters): 9,738 (19.3%) Total basepairs processed: 11,266,789 bp Total written (filtered): 2,596,365 bp (23.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9764 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 5 1 18 7 0.0 3 0 0 3 2 2 19 180 0.0 3 0 140 19 14 7 20 8953 0.0 4 8156 592 127 56 22 21 22 0.0 4 7 11 3 1 22 3 0.0 4 0 0 2 1 23 67 0.0 4 6 2 0 39 20 27 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 36 3 0.0 4 0 0 0 0 3 37 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 1 61 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 78 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 96 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 126 10 0.0 4 0 0 0 0 10 127 478 0.0 4 0 0 0 0 478 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 164 4 0.0 4 0 0 0 0 4 176 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 9,738 Reads with adapters: 2,271 (23.3%) Reads written (passing filters): 9,738 (100.0%) Total basepairs processed: 2,596,365 bp Total written (filtered): 2,573,664 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2271 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 9.1% G: 86.1% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 152.2 0 8 4 4 38.0 0 4 7 1 0.6 0 1 9 632 0.0 0 65 567 10 1331 0.0 1 113 1218 11 268 0.0 1 19 249 12 14 0.0 1 0 14 13 9 0.0 1 0 9 15 1 0.0 1 0 1 50 1 0.0 1 0 1 150 1 0.0 1 1 208 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.