This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.94 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 58,961 (2.3%) Reads that were too short: 21,577 (0.8%) Reads written (passing filters): 37,384 (1.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 9,045,777 bp (1.2%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 58961 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 153 628.5 0 153 7 458 628.5 0 458 8 9467 628.5 0 9467 9 10199 628.5 0 10199 10 65 628.5 0 65 11 43 628.5 0 43 12 47 628.5 0 47 13 47 628.5 0 47 14 52 628.5 0 52 15 40 628.5 0 40 16 54 628.5 0 54 17 42 628.5 0 42 18 37 628.5 0 37 19 37 628.5 0 37 20 48 628.5 0 48 21 54 628.5 0 54 22 52 628.5 0 52 23 42 628.5 0 42 24 43 628.5 0 43 25 41 628.5 0 41 26 48 628.5 0 48 27 51 628.5 0 51 28 53 628.5 0 53 29 36 628.5 0 36 30 47 628.5 0 47 31 56 628.5 0 56 32 58 628.5 0 58 33 61 628.5 0 61 34 42 628.5 0 42 35 40 628.5 0 40 36 63 628.5 0 63 37 52 628.5 0 52 38 105 628.5 0 105 39 55 628.5 0 55 40 77 628.5 0 77 41 66 628.5 0 66 42 52 628.5 0 52 43 47 628.5 0 47 44 46 628.5 0 46 45 50 628.5 0 50 46 40 628.5 0 40 47 66 628.5 0 66 48 57 628.5 0 57 49 28 628.5 0 28 50 48 628.5 0 48 51 68 628.5 0 68 52 59 628.5 0 59 53 58 628.5 0 58 54 48 628.5 0 48 55 62 628.5 0 62 56 63 628.5 0 63 57 46 628.5 0 46 58 46 628.5 0 46 59 58 628.5 0 58 60 94 628.5 0 94 61 61 628.5 0 61 62 68 628.5 0 68 63 73 628.5 0 73 64 62 628.5 0 62 65 50 628.5 0 50 66 54 628.5 0 54 67 72 628.5 0 72 68 71 628.5 0 71 69 61 628.5 0 61 70 64 628.5 0 64 71 67 628.5 0 67 72 59 628.5 0 59 73 44 628.5 0 44 74 128 628.5 0 128 75 66 628.5 0 66 76 54 628.5 0 54 77 56 628.5 0 56 78 82 628.5 0 82 79 65 628.5 0 65 80 62 628.5 0 62 81 51 628.5 0 51 82 79 628.5 0 79 83 56 628.5 0 56 84 88 628.5 0 88 85 54 628.5 0 54 86 59 628.5 0 59 87 77 628.5 0 77 88 82 628.5 0 82 89 67 628.5 0 67 90 46 628.5 0 46 91 96 628.5 0 96 92 63 628.5 0 63 93 71 628.5 0 71 94 124 628.5 0 124 95 103 628.5 0 103 96 93 628.5 0 93 97 439 628.5 0 439 98 207 628.5 0 207 99 296 628.5 0 296 100 139 628.5 0 139 101 61 628.5 0 61 102 54 628.5 0 54 103 53 628.5 0 53 104 63 628.5 0 63 105 64 628.5 0 64 106 71 628.5 0 71 107 69 628.5 0 69 108 91 628.5 0 91 109 107 628.5 0 107 110 89 628.5 0 89 111 689 628.5 0 689 112 757 628.5 0 757 113 641 628.5 0 641 114 52 628.5 0 52 115 43 628.5 0 43 116 59 628.5 0 59 117 57 628.5 0 57 118 57 628.5 0 57 119 60 628.5 0 60 120 52 628.5 0 52 121 41 628.5 0 41 122 61 628.5 0 61 123 55 628.5 0 55 124 53 628.5 0 53 125 67 628.5 0 67 126 53 628.5 0 53 127 69 628.5 0 69 128 70 628.5 0 70 129 78 628.5 0 78 130 60 628.5 0 60 131 66 628.5 0 66 132 91 628.5 0 91 133 70 628.5 0 70 134 135 628.5 0 135 135 60 628.5 0 60 136 65 628.5 0 65 137 49 628.5 0 49 138 70 628.5 0 70 139 59 628.5 0 59 140 54 628.5 0 54 141 70 628.5 0 70 142 54 628.5 0 54 143 46 628.5 0 46 144 66 628.5 0 66 145 80 628.5 0 80 146 90 628.5 0 90 147 99 628.5 0 99 148 80 628.5 0 80 149 66 628.5 0 66 150 49 628.5 0 49 151 51 628.5 0 51 152 70 628.5 0 70 153 53 628.5 0 53 154 119 628.5 0 119 155 149 628.5 0 149 156 329 628.5 0 329 157 384 628.5 0 384 158 61 628.5 0 61 159 37 628.5 0 37 160 58 628.5 0 58 161 65 628.5 0 65 162 60 628.5 0 60 163 52 628.5 0 52 164 81 628.5 0 81 165 65 628.5 0 65 166 138 628.5 0 138 167 103 628.5 0 103 168 97 628.5 0 97 169 44 628.5 0 44 170 49 628.5 0 49 171 46 628.5 0 46 172 57 628.5 0 57 173 48 628.5 0 48 174 67 628.5 0 67 175 45 628.5 0 45 176 56 628.5 0 56 177 58 628.5 0 58 178 58 628.5 0 58 179 71 628.5 0 71 180 82 628.5 0 82 181 85 628.5 0 85 182 64 628.5 0 64 183 62 628.5 0 62 184 56 628.5 0 56 185 57 628.5 0 57 186 50 628.5 0 50 187 69 628.5 0 69 188 62 628.5 0 62 189 67 628.5 0 67 190 72 628.5 0 72 191 76 628.5 0 76 192 79 628.5 0 79 193 146 628.5 0 146 194 615 628.5 0 615 195 602 628.5 0 602 196 346 628.5 0 346 197 67 628.5 0 67 198 68 628.5 0 68 199 71 628.5 0 71 200 119 628.5 0 119 201 107 628.5 0 107 202 107 628.5 0 107 203 49 628.5 0 49 204 53 628.5 0 53 205 63 628.5 0 63 206 69 628.5 0 69 207 61 628.5 0 61 208 64 628.5 0 64 209 74 628.5 0 74 210 63 628.5 0 63 211 62 628.5 0 62 212 49 628.5 0 49 213 63 628.5 0 63 214 65 628.5 0 65 215 74 628.5 0 74 216 73 628.5 0 73 217 80 628.5 0 80 218 61 628.5 0 61 219 56 628.5 0 56 220 210 628.5 0 210 221 212 628.5 0 212 222 283 628.5 0 283 223 326 628.5 0 326 224 64 628.5 0 64 225 59 628.5 0 59 226 63 628.5 0 63 227 64 628.5 0 64 228 71 628.5 0 71 229 71 628.5 0 71 230 56 628.5 0 56 231 46 628.5 0 46 232 49 628.5 0 49 233 67 628.5 0 67 234 63 628.5 0 63 235 66 628.5 0 66 236 64 628.5 0 64 237 90 628.5 0 90 238 169 628.5 0 169 239 575 628.5 0 575 240 103 628.5 0 103 241 191 628.5 0 191 242 113 628.5 0 113 243 391 628.5 0 391 244 158 628.5 0 158 245 201 628.5 0 201 246 96 628.5 0 96 247 70 628.5 0 70 248 91 628.5 0 91 249 118 628.5 0 118 250 106 628.5 0 106 251 119 628.5 0 119 252 147 628.5 0 147 253 172 628.5 0 172 254 203 628.5 0 203 255 210 628.5 0 210 256 415 628.5 0 415 257 92 628.5 0 92 258 92 628.5 0 92 259 105 628.5 0 105 260 139 628.5 0 139 261 155 628.5 0 155 262 1767 628.5 0 1767 263 2162 628.5 0 2162 264 4798 628.5 0 4798 265 137 628.5 0 137 266 64 628.5 0 64 267 64 628.5 0 64 268 62 628.5 0 62 269 75 628.5 0 75 270 74 628.5 0 74 271 85 628.5 0 85 272 81 628.5 0 81 273 95 628.5 0 95 274 75 628.5 0 75 275 91 628.5 0 91 276 76 628.5 0 76 277 72 628.5 0 72 278 60 628.5 0 60 279 104 628.5 0 104 280 163 628.5 0 163 281 111 628.5 0 111 282 113 628.5 0 113 283 97 628.5 0 97 284 72 628.5 0 72 285 79 628.5 0 79 286 96 628.5 0 96 287 91 628.5 0 91 288 82 628.5 0 82 289 131 628.5 0 131 290 134 628.5 0 134 291 161 628.5 0 161 292 133 628.5 0 133 293 940 628.5 0 940 294 1038 628.5 0 1038 295 276 628.5 0 276 296 76 628.5 0 76 297 90 628.5 0 90 298 92 628.5 0 92 299 109 628.5 0 109 300 122 628.5 0 122 301 88 628.5 0 88 Finished in 1.70 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 37,384 Reads with adapters: 9,506 (25.4%) Reads that were too short: 43 (0.1%) Reads written (passing filters): 9,463 (25.3%) Total basepairs processed: 9,045,777 bp Total written (filtered): 2,608,228 bp (28.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9506 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 80 0.0 3 0 78 2 17 9320 0.0 3 9151 164 5 18 21 0.0 3 0 20 1 19 4 0.0 3 1 0 3 20 10 0.0 3 1 0 0 9 32 1 0.0 3 0 0 0 1 38 1 0.0 3 1 55 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 76 10 0.0 3 0 0 0 10 77 4 0.0 3 0 0 0 4 93 4 0.0 3 0 0 0 4 94 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 188 36 0.0 3 0 0 1 35 189 3 0.0 3 0 0 0 3 217 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 9,463 Reads with adapters: 57 (0.6%) Reads written (passing filters): 9,463 (100.0%) Total basepairs processed: 2,608,228 bp Total written (filtered): 2,608,010 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 57 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.5% C: 5.3% G: 73.7% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 10 147.9 0 10 4 47 37.0 0 47