This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.16 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 207,620 (8.1%) Reads that were too short: 21,809 (0.8%) Reads written (passing filters): 185,811 (7.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 40,234,408 bp (5.2%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 207620 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 854 628.5 0 854 7 906 628.5 0 906 8 785 628.5 0 785 9 125 628.5 0 125 10 285 628.5 0 285 11 191 628.5 0 191 12 184 628.5 0 184 13 147 628.5 0 147 14 110 628.5 0 110 15 126 628.5 0 126 16 115 628.5 0 115 17 110 628.5 0 110 18 124 628.5 0 124 19 182 628.5 0 182 20 137 628.5 0 137 21 150 628.5 0 150 22 144 628.5 0 144 23 137 628.5 0 137 24 129 628.5 0 129 25 135 628.5 0 135 26 126 628.5 0 126 27 97 628.5 0 97 28 150 628.5 0 150 29 290 628.5 0 290 30 108 628.5 0 108 31 110 628.5 0 110 32 101 628.5 0 101 33 113 628.5 0 113 34 106 628.5 0 106 35 125 628.5 0 125 36 143 628.5 0 143 37 151 628.5 0 151 38 225 628.5 0 225 39 357 628.5 0 357 40 169 628.5 0 169 41 259 628.5 0 259 42 169 628.5 0 169 43 169 628.5 0 169 44 274 628.5 0 274 45 173 628.5 0 173 46 184 628.5 0 184 47 169 628.5 0 169 48 224 628.5 0 224 49 401 628.5 0 401 50 386 628.5 0 386 51 584 628.5 0 584 52 736 628.5 0 736 53 889 628.5 0 889 54 2171 628.5 0 2171 55 4093 628.5 0 4093 56 42343 628.5 0 42343 57 22631 628.5 0 22631 58 45988 628.5 0 45988 59 292 628.5 0 292 60 161 628.5 0 161 61 181 628.5 0 181 62 554 628.5 0 554 63 221 628.5 0 221 64 486 628.5 0 486 65 96 628.5 0 96 66 128 628.5 0 128 67 121 628.5 0 121 68 114 628.5 0 114 69 132 628.5 0 132 70 140 628.5 0 140 71 117 628.5 0 117 72 118 628.5 0 118 73 113 628.5 0 113 74 136 628.5 0 136 75 112 628.5 0 112 76 96 628.5 0 96 77 81 628.5 0 81 78 77 628.5 0 77 79 117 628.5 0 117 80 149 628.5 0 149 81 146 628.5 0 146 82 124 628.5 0 124 83 186 628.5 0 186 84 161 628.5 0 161 85 176 628.5 0 176 86 156 628.5 0 156 87 146 628.5 0 146 88 206 628.5 0 206 89 172 628.5 0 172 90 115 628.5 0 115 91 85 628.5 0 85 92 83 628.5 0 83 93 97 628.5 0 97 94 78 628.5 0 78 95 107 628.5 0 107 96 87 628.5 0 87 97 102 628.5 0 102 98 73 628.5 0 73 99 96 628.5 0 96 100 106 628.5 0 106 101 101 628.5 0 101 102 89 628.5 0 89 103 211 628.5 0 211 104 328 628.5 0 328 105 316 628.5 0 316 106 91 628.5 0 91 107 86 628.5 0 86 108 105 628.5 0 105 109 97 628.5 0 97 110 99 628.5 0 99 111 108 628.5 0 108 112 91 628.5 0 91 113 219 628.5 0 219 114 154 628.5 0 154 115 107 628.5 0 107 116 97 628.5 0 97 117 127 628.5 0 127 118 117 628.5 0 117 119 405 628.5 0 405 120 108 628.5 0 108 121 106 628.5 0 106 122 81 628.5 0 81 123 141 628.5 0 141 124 396 628.5 0 396 125 809 628.5 0 809 126 821 628.5 0 821 127 182 628.5 0 182 128 130 628.5 0 130 129 140 628.5 0 140 130 151 628.5 0 151 131 98 628.5 0 98 132 128 628.5 0 128 133 104 628.5 0 104 134 93 628.5 0 93 135 118 628.5 0 118 136 106 628.5 0 106 137 97 628.5 0 97 138 143 628.5 0 143 139 124 628.5 0 124 140 252 628.5 0 252 141 466 628.5 0 466 142 222 628.5 0 222 143 196 628.5 0 196 144 189 628.5 0 189 145 330 628.5 0 330 146 493 628.5 0 493 147 3636 628.5 0 3636 148 1681 628.5 0 1681 149 2976 628.5 0 2976 150 1149 628.5 0 1149 151 333 628.5 0 333 152 943 628.5 0 943 153 190 628.5 0 190 154 156 628.5 0 156 155 131 628.5 0 131 156 152 628.5 0 152 157 128 628.5 0 128 158 133 628.5 0 133 159 192 628.5 0 192 160 878 628.5 0 878 161 603 628.5 0 603 162 495 628.5 0 495 163 163 628.5 0 163 164 155 628.5 0 155 165 302 628.5 0 302 166 466 628.5 0 466 167 5229 628.5 0 5229 168 1207 628.5 0 1207 169 3807 628.5 0 3807 170 190 628.5 0 190 171 309 628.5 0 309 172 309 628.5 0 309 173 321 628.5 0 321 174 307 628.5 0 307 175 606 628.5 0 606 176 642 628.5 0 642 177 878 628.5 0 878 178 207 628.5 0 207 179 126 628.5 0 126 180 173 628.5 0 173 181 177 628.5 0 177 182 167 628.5 0 167 183 186 628.5 0 186 184 143 628.5 0 143 185 154 628.5 0 154 186 183 628.5 0 183 187 238 628.5 0 238 188 202 628.5 0 202 189 139 628.5 0 139 190 162 628.5 0 162 191 184 628.5 0 184 192 246 628.5 0 246 193 242 628.5 0 242 194 334 628.5 0 334 195 965 628.5 0 965 196 1197 628.5 0 1197 197 1003 628.5 0 1003 198 763 628.5 0 763 199 456 628.5 0 456 200 3020 628.5 0 3020 201 1771 628.5 0 1771 202 2284 628.5 0 2284 203 190 628.5 0 190 204 79 628.5 0 79 205 111 628.5 0 111 206 120 628.5 0 120 207 172 628.5 0 172 208 135 628.5 0 135 209 100 628.5 0 100 210 80 628.5 0 80 211 86 628.5 0 86 212 125 628.5 0 125 213 102 628.5 0 102 214 116 628.5 0 116 215 117 628.5 0 117 216 112 628.5 0 112 217 101 628.5 0 101 218 104 628.5 0 104 219 262 628.5 0 262 220 260 628.5 0 260 221 344 628.5 0 344 222 134 628.5 0 134 223 86 628.5 0 86 224 73 628.5 0 73 225 123 628.5 0 123 226 95 628.5 0 95 227 94 628.5 0 94 228 168 628.5 0 168 229 429 628.5 0 429 230 292 628.5 0 292 231 336 628.5 0 336 232 692 628.5 0 692 233 378 628.5 0 378 234 522 628.5 0 522 235 85 628.5 0 85 236 94 628.5 0 94 237 105 628.5 0 105 238 107 628.5 0 107 239 121 628.5 0 121 240 142 628.5 0 142 241 156 628.5 0 156 242 170 628.5 0 170 243 148 628.5 0 148 244 86 628.5 0 86 245 96 628.5 0 96 246 111 628.5 0 111 247 106 628.5 0 106 248 175 628.5 0 175 249 134 628.5 0 134 250 166 628.5 0 166 251 119 628.5 0 119 252 113 628.5 0 113 253 90 628.5 0 90 254 83 628.5 0 83 255 85 628.5 0 85 256 94 628.5 0 94 257 111 628.5 0 111 258 151 628.5 0 151 259 106 628.5 0 106 260 126 628.5 0 126 261 174 628.5 0 174 262 185 628.5 0 185 263 144 628.5 0 144 264 98 628.5 0 98 265 92 628.5 0 92 266 148 628.5 0 148 267 174 628.5 0 174 268 341 628.5 0 341 269 421 628.5 0 421 270 422 628.5 0 422 271 226 628.5 0 226 272 123 628.5 0 123 273 215 628.5 0 215 274 172 628.5 0 172 275 191 628.5 0 191 276 244 628.5 0 244 277 194 628.5 0 194 278 328 628.5 0 328 279 258 628.5 0 258 280 258 628.5 0 258 281 424 628.5 0 424 282 472 628.5 0 472 283 426 628.5 0 426 284 153 628.5 0 153 285 89 628.5 0 89 286 95 628.5 0 95 287 172 628.5 0 172 288 244 628.5 0 244 289 358 628.5 0 358 290 260 628.5 0 260 291 430 628.5 0 430 292 245 628.5 0 245 293 345 628.5 0 345 294 387 628.5 0 387 295 275 628.5 0 275 296 162 628.5 0 162 297 143 628.5 0 143 298 111 628.5 0 111 299 126 628.5 0 126 300 358 628.5 0 358 301 624 628.5 0 624 Finished in 7.80 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 185,811 Reads with adapters: 1,497 (0.8%) Reads that were too short: 62 (0.0%) Reads written (passing filters): 1,435 (0.8%) Total basepairs processed: 40,234,408 bp Total written (filtered): 383,127 bp (1.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1497 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 0 1 19 9 0.0 3 0 6 1 2 20 674 0.0 4 614 49 9 1 1 21 5 0.0 4 3 1 1 22 4 0.0 4 0 0 2 2 23 669 0.0 4 597 50 13 5 4 24 1 0.0 4 1 25 1 0.0 4 0 0 1 26 7 0.0 4 0 0 0 3 4 27 1 0.0 4 0 1 45 4 0.0 4 0 0 0 0 4 46 4 0.0 4 0 0 0 1 3 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 70 3 0.0 4 0 0 0 1 2 71 1 0.0 4 0 0 0 0 1 79 11 0.0 4 0 0 0 0 11 83 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 1 113 1 0.0 4 0 0 0 0 1 122 17 0.0 4 0 0 0 1 16 123 8 0.0 4 0 0 0 0 8 126 3 0.0 4 0 0 0 0 3 127 16 0.0 4 0 0 0 0 16 147 1 0.0 4 0 0 1 148 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 155 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 2 0.0 4 0 0 0 0 2 169 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 1 188 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 27 0.0 4 0 0 0 2 25 220 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 1,435 Reads with adapters: 357 (24.9%) Reads written (passing filters): 1,435 (100.0%) Total basepairs processed: 383,127 bp Total written (filtered): 376,880 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 357 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 8.4% G: 86.0% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 22.4 0 1 4 1 5.6 0 1 9 41 0.0 0 8 33 10 105 0.0 1 11 94 11 189 0.0 1 6 183 13 1 0.0 1 0 1 90 8 0.0 1 8 91 1 0.0 1 0 1 92 1 0.0 1 1 151 1 0.0 1 0 1 177 3 0.0 1 3 180 1 0.0 1 1 215 2 0.0 1 2 267 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.