This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.96 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 207,913 (8.1%) Reads that were too short: 16,046 (0.6%) Reads written (passing filters): 191,867 (7.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 42,687,520 bp (5.5%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 207913 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 873 628.5 0 873 7 834 628.5 0 834 8 913 628.5 0 913 9 125 628.5 0 125 10 138 628.5 0 138 11 110 628.5 0 110 12 134 628.5 0 134 13 128 628.5 0 128 14 104 628.5 0 104 15 119 628.5 0 119 16 121 628.5 0 121 17 112 628.5 0 112 18 157 628.5 0 157 19 166 628.5 0 166 20 159 628.5 0 159 21 153 628.5 0 153 22 157 628.5 0 157 23 148 628.5 0 148 24 142 628.5 0 142 25 130 628.5 0 130 26 158 628.5 0 158 27 112 628.5 0 112 28 181 628.5 0 181 29 269 628.5 0 269 30 105 628.5 0 105 31 90 628.5 0 90 32 78 628.5 0 78 33 112 628.5 0 112 34 122 628.5 0 122 35 137 628.5 0 137 36 197 628.5 0 197 37 150 628.5 0 150 38 252 628.5 0 252 39 460 628.5 0 460 40 190 628.5 0 190 41 241 628.5 0 241 42 171 628.5 0 171 43 240 628.5 0 240 44 325 628.5 0 325 45 202 628.5 0 202 46 221 628.5 0 221 47 166 628.5 0 166 48 193 628.5 0 193 49 376 628.5 0 376 50 351 628.5 0 351 51 589 628.5 0 589 52 780 628.5 0 780 53 938 628.5 0 938 54 2192 628.5 0 2192 55 3293 628.5 0 3293 56 47985 628.5 0 47985 57 25877 628.5 0 25877 58 55046 628.5 0 55046 59 287 628.5 0 287 60 124 628.5 0 124 61 106 628.5 0 106 62 174 628.5 0 174 63 135 628.5 0 135 64 142 628.5 0 142 65 87 628.5 0 87 66 108 628.5 0 108 67 121 628.5 0 121 68 93 628.5 0 93 69 123 628.5 0 123 70 105 628.5 0 105 71 127 628.5 0 127 72 116 628.5 0 116 73 112 628.5 0 112 74 129 628.5 0 129 75 114 628.5 0 114 76 103 628.5 0 103 77 80 628.5 0 80 78 84 628.5 0 84 79 128 628.5 0 128 80 134 628.5 0 134 81 126 628.5 0 126 82 107 628.5 0 107 83 132 628.5 0 132 84 112 628.5 0 112 85 94 628.5 0 94 86 107 628.5 0 107 87 104 628.5 0 104 88 97 628.5 0 97 89 93 628.5 0 93 90 101 628.5 0 101 91 68 628.5 0 68 92 96 628.5 0 96 93 79 628.5 0 79 94 81 628.5 0 81 95 60 628.5 0 60 96 102 628.5 0 102 97 85 628.5 0 85 98 77 628.5 0 77 99 94 628.5 0 94 100 116 628.5 0 116 101 94 628.5 0 94 102 95 628.5 0 95 103 169 628.5 0 169 104 184 628.5 0 184 105 188 628.5 0 188 106 92 628.5 0 92 107 89 628.5 0 89 108 114 628.5 0 114 109 110 628.5 0 110 110 115 628.5 0 115 111 136 628.5 0 136 112 82 628.5 0 82 113 127 628.5 0 127 114 121 628.5 0 121 115 96 628.5 0 96 116 113 628.5 0 113 117 97 628.5 0 97 118 105 628.5 0 105 119 456 628.5 0 456 120 125 628.5 0 125 121 108 628.5 0 108 122 110 628.5 0 110 123 114 628.5 0 114 124 312 628.5 0 312 125 596 628.5 0 596 126 695 628.5 0 695 127 142 628.5 0 142 128 123 628.5 0 123 129 100 628.5 0 100 130 118 628.5 0 118 131 90 628.5 0 90 132 109 628.5 0 109 133 105 628.5 0 105 134 105 628.5 0 105 135 121 628.5 0 121 136 92 628.5 0 92 137 109 628.5 0 109 138 138 628.5 0 138 139 138 628.5 0 138 140 179 628.5 0 179 141 465 628.5 0 465 142 175 628.5 0 175 143 142 628.5 0 142 144 167 628.5 0 167 145 180 628.5 0 180 146 276 628.5 0 276 147 1598 628.5 0 1598 148 974 628.5 0 974 149 1688 628.5 0 1688 150 828 628.5 0 828 151 236 628.5 0 236 152 842 628.5 0 842 153 115 628.5 0 115 154 120 628.5 0 120 155 106 628.5 0 106 156 185 628.5 0 185 157 119 628.5 0 119 158 108 628.5 0 108 159 151 628.5 0 151 160 488 628.5 0 488 161 256 628.5 0 256 162 252 628.5 0 252 163 198 628.5 0 198 164 147 628.5 0 147 165 318 628.5 0 318 166 425 628.5 0 425 167 5764 628.5 0 5764 168 899 628.5 0 899 169 3962 628.5 0 3962 170 146 628.5 0 146 171 191 628.5 0 191 172 143 628.5 0 143 173 192 628.5 0 192 174 212 628.5 0 212 175 215 628.5 0 215 176 309 628.5 0 309 177 273 628.5 0 273 178 122 628.5 0 122 179 81 628.5 0 81 180 150 628.5 0 150 181 131 628.5 0 131 182 110 628.5 0 110 183 149 628.5 0 149 184 95 628.5 0 95 185 130 628.5 0 130 186 158 628.5 0 158 187 228 628.5 0 228 188 137 628.5 0 137 189 125 628.5 0 125 190 141 628.5 0 141 191 115 628.5 0 115 192 149 628.5 0 149 193 157 628.5 0 157 194 129 628.5 0 129 195 356 628.5 0 356 196 378 628.5 0 378 197 360 628.5 0 360 198 374 628.5 0 374 199 522 628.5 0 522 200 4803 628.5 0 4803 201 2205 628.5 0 2205 202 3193 628.5 0 3193 203 187 628.5 0 187 204 80 628.5 0 80 205 105 628.5 0 105 206 96 628.5 0 96 207 107 628.5 0 107 208 123 628.5 0 123 209 108 628.5 0 108 210 67 628.5 0 67 211 83 628.5 0 83 212 108 628.5 0 108 213 117 628.5 0 117 214 80 628.5 0 80 215 90 628.5 0 90 216 118 628.5 0 118 217 99 628.5 0 99 218 75 628.5 0 75 219 137 628.5 0 137 220 129 628.5 0 129 221 132 628.5 0 132 222 104 628.5 0 104 223 98 628.5 0 98 224 76 628.5 0 76 225 88 628.5 0 88 226 108 628.5 0 108 227 121 628.5 0 121 228 129 628.5 0 129 229 199 628.5 0 199 230 178 628.5 0 178 231 220 628.5 0 220 232 268 628.5 0 268 233 162 628.5 0 162 234 236 628.5 0 236 235 99 628.5 0 99 236 94 628.5 0 94 237 96 628.5 0 96 238 98 628.5 0 98 239 97 628.5 0 97 240 116 628.5 0 116 241 123 628.5 0 123 242 126 628.5 0 126 243 140 628.5 0 140 244 107 628.5 0 107 245 86 628.5 0 86 246 100 628.5 0 100 247 108 628.5 0 108 248 112 628.5 0 112 249 125 628.5 0 125 250 107 628.5 0 107 251 91 628.5 0 91 252 134 628.5 0 134 253 104 628.5 0 104 254 93 628.5 0 93 255 94 628.5 0 94 256 111 628.5 0 111 257 91 628.5 0 91 258 110 628.5 0 110 259 109 628.5 0 109 260 115 628.5 0 115 261 94 628.5 0 94 262 111 628.5 0 111 263 78 628.5 0 78 264 76 628.5 0 76 265 114 628.5 0 114 266 106 628.5 0 106 267 89 628.5 0 89 268 165 628.5 0 165 269 164 628.5 0 164 270 143 628.5 0 143 271 124 628.5 0 124 272 109 628.5 0 109 273 232 628.5 0 232 274 120 628.5 0 120 275 160 628.5 0 160 276 183 628.5 0 183 277 161 628.5 0 161 278 222 628.5 0 222 279 212 628.5 0 212 280 156 628.5 0 156 281 222 628.5 0 222 282 279 628.5 0 279 283 282 628.5 0 282 284 101 628.5 0 101 285 118 628.5 0 118 286 103 628.5 0 103 287 101 628.5 0 101 288 157 628.5 0 157 289 198 628.5 0 198 290 143 628.5 0 143 291 154 628.5 0 154 292 97 628.5 0 97 293 135 628.5 0 135 294 154 628.5 0 154 295 131 628.5 0 131 296 119 628.5 0 119 297 102 628.5 0 102 298 106 628.5 0 106 299 131 628.5 0 131 300 163 628.5 0 163 301 224 628.5 0 224 Finished in 7.25 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 191,867 Reads with adapters: 843 (0.4%) Reads that were too short: 64 (0.0%) Reads written (passing filters): 779 (0.4%) Total basepairs processed: 42,687,520 bp Total written (filtered): 208,083 bp (0.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 843 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 3 0.0 3 0 3 17 719 0.0 3 700 19 18 2 0.0 3 1 1 19 2 0.0 3 0 0 1 1 20 5 0.0 3 0 0 0 5 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 25 1 0.0 3 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 1 1 35 1 0.0 3 0 0 1 39 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 1 51 4 0.0 3 0 0 0 4 52 1 0.0 3 0 0 0 1 53 11 0.0 3 0 0 0 11 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 1 88 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 128 1 0.0 3 1 141 1 0.0 3 0 0 0 1 142 3 0.0 3 1 0 0 2 143 2 0.0 3 0 1 0 1 144 2 0.0 3 0 1 0 1 145 13 0.0 3 0 0 0 13 165 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 1 168 4 0.0 3 0 0 0 4 172 2 0.0 3 0 0 0 2 207 1 0.0 3 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 231 3 0.0 3 0 0 0 3 232 2 0.0 3 0 0 0 2 233 3 0.0 3 0 0 0 3 234 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 0 2 263 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 779 Reads with adapters: 1 (0.1%) Reads written (passing filters): 779 (100.0%) Total basepairs processed: 208,083 bp Total written (filtered): 208,079 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 1 3.0 0 1