This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.57 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 127,438 (4.9%) Reads that were too short: 26,512 (1.0%) Reads written (passing filters): 100,926 (3.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 24,433,951 bp (3.2%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 127438 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3222 628.5 0 3222 7 5978 628.5 0 5978 8 156 628.5 0 156 9 6385 628.5 0 6385 10 72 628.5 0 72 11 53 628.5 0 53 12 119 628.5 0 119 13 83 628.5 0 83 14 76 628.5 0 76 15 92 628.5 0 92 16 103 628.5 0 103 17 83 628.5 0 83 18 94 628.5 0 94 19 80 628.5 0 80 20 97 628.5 0 97 21 98 628.5 0 98 22 86 628.5 0 86 23 81 628.5 0 81 24 93 628.5 0 93 25 90 628.5 0 90 26 103 628.5 0 103 27 140 628.5 0 140 28 157 628.5 0 157 29 196 628.5 0 196 30 141 628.5 0 141 31 144 628.5 0 144 32 320 628.5 0 320 33 202 628.5 0 202 34 158 628.5 0 158 35 142 628.5 0 142 36 153 628.5 0 153 37 160 628.5 0 160 38 222 628.5 0 222 39 212 628.5 0 212 40 266 628.5 0 266 41 339 628.5 0 339 42 5734 628.5 0 5734 43 6525 628.5 0 6525 44 4526 628.5 0 4526 45 120 628.5 0 120 46 93 628.5 0 93 47 169 628.5 0 169 48 193 628.5 0 193 49 155 628.5 0 155 50 152 628.5 0 152 51 101 628.5 0 101 52 143 628.5 0 143 53 176 628.5 0 176 54 113 628.5 0 113 55 151 628.5 0 151 56 104 628.5 0 104 57 130 628.5 0 130 58 171 628.5 0 171 59 152 628.5 0 152 60 259 628.5 0 259 61 381 628.5 0 381 62 422 628.5 0 422 63 776 628.5 0 776 64 1263 628.5 0 1263 65 16454 628.5 0 16454 66 9068 628.5 0 9068 67 21493 628.5 0 21493 68 161 628.5 0 161 69 80 628.5 0 80 70 71 628.5 0 71 71 69 628.5 0 69 72 106 628.5 0 106 73 80 628.5 0 80 74 72 628.5 0 72 75 87 628.5 0 87 76 99 628.5 0 99 77 134 628.5 0 134 78 79 628.5 0 79 79 88 628.5 0 88 80 75 628.5 0 75 81 73 628.5 0 73 82 83 628.5 0 83 83 67 628.5 0 67 84 75 628.5 0 75 85 75 628.5 0 75 86 56 628.5 0 56 87 164 628.5 0 164 88 85 628.5 0 85 89 67 628.5 0 67 90 55 628.5 0 55 91 76 628.5 0 76 92 71 628.5 0 71 93 76 628.5 0 76 94 86 628.5 0 86 95 99 628.5 0 99 96 74 628.5 0 74 97 63 628.5 0 63 98 73 628.5 0 73 99 97 628.5 0 97 100 132 628.5 0 132 101 141 628.5 0 141 102 206 628.5 0 206 103 81 628.5 0 81 104 72 628.5 0 72 105 73 628.5 0 73 106 83 628.5 0 83 107 92 628.5 0 92 108 85 628.5 0 85 109 55 628.5 0 55 110 114 628.5 0 114 111 73 628.5 0 73 112 86 628.5 0 86 113 75 628.5 0 75 114 59 628.5 0 59 115 82 628.5 0 82 116 63 628.5 0 63 117 62 628.5 0 62 118 82 628.5 0 82 119 82 628.5 0 82 120 77 628.5 0 77 121 95 628.5 0 95 122 60 628.5 0 60 123 76 628.5 0 76 124 92 628.5 0 92 125 61 628.5 0 61 126 61 628.5 0 61 127 63 628.5 0 63 128 79 628.5 0 79 129 91 628.5 0 91 130 96 628.5 0 96 131 86 628.5 0 86 132 94 628.5 0 94 133 92 628.5 0 92 134 308 628.5 0 308 135 279 628.5 0 279 136 294 628.5 0 294 137 126 628.5 0 126 138 137 628.5 0 137 139 88 628.5 0 88 140 101 628.5 0 101 141 69 628.5 0 69 142 63 628.5 0 63 143 61 628.5 0 61 144 54 628.5 0 54 145 76 628.5 0 76 146 76 628.5 0 76 147 77 628.5 0 77 148 86 628.5 0 86 149 65 628.5 0 65 150 38 628.5 0 38 151 61 628.5 0 61 152 56 628.5 0 56 153 60 628.5 0 60 154 54 628.5 0 54 155 46 628.5 0 46 156 66 628.5 0 66 157 75 628.5 0 75 158 130 628.5 0 130 159 127 628.5 0 127 160 79 628.5 0 79 161 86 628.5 0 86 162 72 628.5 0 72 163 60 628.5 0 60 164 63 628.5 0 63 165 68 628.5 0 68 166 63 628.5 0 63 167 71 628.5 0 71 168 88 628.5 0 88 169 72 628.5 0 72 170 87 628.5 0 87 171 48 628.5 0 48 172 55 628.5 0 55 173 64 628.5 0 64 174 81 628.5 0 81 175 70 628.5 0 70 176 53 628.5 0 53 177 73 628.5 0 73 178 95 628.5 0 95 179 124 628.5 0 124 180 126 628.5 0 126 181 110 628.5 0 110 182 99 628.5 0 99 183 117 628.5 0 117 184 125 628.5 0 125 185 88 628.5 0 88 186 107 628.5 0 107 187 41 628.5 0 41 188 49 628.5 0 49 189 75 628.5 0 75 190 64 628.5 0 64 191 68 628.5 0 68 192 70 628.5 0 70 193 48 628.5 0 48 194 54 628.5 0 54 195 72 628.5 0 72 196 107 628.5 0 107 197 112 628.5 0 112 198 120 628.5 0 120 199 84 628.5 0 84 200 86 628.5 0 86 201 102 628.5 0 102 202 169 628.5 0 169 203 189 628.5 0 189 204 155 628.5 0 155 205 71 628.5 0 71 206 55 628.5 0 55 207 94 628.5 0 94 208 75 628.5 0 75 209 89 628.5 0 89 210 63 628.5 0 63 211 70 628.5 0 70 212 57 628.5 0 57 213 91 628.5 0 91 214 61 628.5 0 61 215 83 628.5 0 83 216 112 628.5 0 112 217 112 628.5 0 112 218 112 628.5 0 112 219 106 628.5 0 106 220 106 628.5 0 106 221 137 628.5 0 137 222 509 628.5 0 509 223 629 628.5 0 629 224 981 628.5 0 981 225 1093 628.5 0 1093 226 81 628.5 0 81 227 83 628.5 0 83 228 93 628.5 0 93 229 63 628.5 0 63 230 69 628.5 0 69 231 62 628.5 0 62 232 72 628.5 0 72 233 87 628.5 0 87 234 59 628.5 0 59 235 83 628.5 0 83 236 95 628.5 0 95 237 87 628.5 0 87 238 88 628.5 0 88 239 75 628.5 0 75 240 94 628.5 0 94 241 90 628.5 0 90 242 284 628.5 0 284 243 330 628.5 0 330 244 299 628.5 0 299 245 267 628.5 0 267 246 104 628.5 0 104 247 92 628.5 0 92 248 82 628.5 0 82 249 85 628.5 0 85 250 113 628.5 0 113 251 179 628.5 0 179 252 176 628.5 0 176 253 356 628.5 0 356 254 440 628.5 0 440 255 4370 628.5 0 4370 256 922 628.5 0 922 257 2487 628.5 0 2487 258 306 628.5 0 306 259 122 628.5 0 122 260 801 628.5 0 801 261 1169 628.5 0 1169 262 492 628.5 0 492 263 292 628.5 0 292 264 155 628.5 0 155 265 148 628.5 0 148 266 219 628.5 0 219 267 114 628.5 0 114 268 67 628.5 0 67 269 111 628.5 0 111 270 392 628.5 0 392 271 801 628.5 0 801 272 1455 628.5 0 1455 273 102 628.5 0 102 274 94 628.5 0 94 275 68 628.5 0 68 276 100 628.5 0 100 277 138 628.5 0 138 278 147 628.5 0 147 279 225 628.5 0 225 280 112 628.5 0 112 281 69 628.5 0 69 282 42 628.5 0 42 283 73 628.5 0 73 284 70 628.5 0 70 285 70 628.5 0 70 286 54 628.5 0 54 287 61 628.5 0 61 288 51 628.5 0 51 289 50 628.5 0 50 290 87 628.5 0 87 291 85 628.5 0 85 292 129 628.5 0 129 293 114 628.5 0 114 294 239 628.5 0 239 295 42 628.5 0 42 296 65 628.5 0 65 297 73 628.5 0 73 298 76 628.5 0 76 299 112 628.5 0 112 300 111 628.5 0 111 301 123 628.5 0 123 Finished in 4.82 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 100,926 Reads with adapters: 8,049 (8.0%) Reads that were too short: 121 (0.1%) Reads written (passing filters): 7,928 (7.9%) Total basepairs processed: 24,433,951 bp Total written (filtered): 2,130,539 bp (8.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8049 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 2 1 19 87 0.0 3 0 66 12 7 2 20 5809 0.0 4 5415 314 61 15 4 21 12 0.0 4 1 7 2 2 22 34 0.0 4 5 19 6 4 23 1633 0.0 4 1464 128 26 12 3 24 5 0.0 4 0 3 0 0 2 26 13 0.0 4 0 0 0 5 8 28 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 54 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 1 66 1 0.0 4 0 0 1 79 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 1 92 48 0.0 4 0 0 0 0 48 94 1 0.0 4 0 0 0 1 98 1 0.0 4 0 0 0 0 1 107 12 0.0 4 0 0 0 0 12 112 1 0.0 4 0 0 0 0 1 113 28 0.0 4 0 0 0 0 28 114 2 0.0 4 0 0 0 0 2 115 10 0.0 4 0 0 0 0 10 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 201 0.0 4 0 0 0 0 201 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 10 0.0 4 0 0 0 1 9 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 158 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 2 0.0 4 0 0 0 0 2 176 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 0 2 192 1 0.0 4 0 0 0 0 1 193 38 0.0 4 0 0 0 0 38 194 43 0.0 4 0 0 0 1 42 195 4 0.0 4 0 0 0 0 4 196 2 0.0 4 0 0 0 0 2 199 3 0.0 4 0 0 0 0 3 202 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 4 0.0 4 0 0 0 0 4 230 2 0.0 4 0 0 0 0 2 247 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 7,928 Reads with adapters: 800 (10.1%) Reads written (passing filters): 7,928 (100.0%) Total basepairs processed: 2,130,539 bp Total written (filtered): 2,118,959 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 800 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.1% C: 14.4% G: 78.9% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26 123.9 0 26 4 6 31.0 0 6 6 1 1.9 0 1 8 1 0.1 0 1 9 71 0.0 0 9 62 10 301 0.0 1 19 282 11 267 0.0 1 15 252 12 107 0.0 1 2 105 13 4 0.0 1 0 4 124 1 0.0 1 1 152 2 0.0 1 1 1 171 1 0.0 1 0 1 189 1 0.0 1 0 1 208 1 0.0 1 1 210 1 0.0 1 1 223 1 0.0 1 1 264 7 0.0 1 7 265 1 0.0 1 1