This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.23 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 134,079 (5.2%) Reads that were too short: 18,080 (0.7%) Reads written (passing filters): 115,999 (4.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 27,942,224 bp (3.6%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 134079 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3687 628.5 0 3687 7 7168 628.5 0 7168 8 165 628.5 0 165 9 6232 628.5 0 6232 10 97 628.5 0 97 11 99 628.5 0 99 12 151 628.5 0 151 13 141 628.5 0 141 14 112 628.5 0 112 15 110 628.5 0 110 16 193 628.5 0 193 17 159 628.5 0 159 18 150 628.5 0 150 19 116 628.5 0 116 20 177 628.5 0 177 21 163 628.5 0 163 22 140 628.5 0 140 23 178 628.5 0 178 24 176 628.5 0 176 25 158 628.5 0 158 26 141 628.5 0 141 27 163 628.5 0 163 28 197 628.5 0 197 29 251 628.5 0 251 30 211 628.5 0 211 31 196 628.5 0 196 32 351 628.5 0 351 33 236 628.5 0 236 34 167 628.5 0 167 35 195 628.5 0 195 36 200 628.5 0 200 37 193 628.5 0 193 38 290 628.5 0 290 39 265 628.5 0 265 40 302 628.5 0 302 41 300 628.5 0 300 42 5704 628.5 0 5704 43 6592 628.5 0 6592 44 4522 628.5 0 4522 45 165 628.5 0 165 46 170 628.5 0 170 47 219 628.5 0 219 48 313 628.5 0 313 49 194 628.5 0 194 50 192 628.5 0 192 51 190 628.5 0 190 52 174 628.5 0 174 53 230 628.5 0 230 54 188 628.5 0 188 55 206 628.5 0 206 56 139 628.5 0 139 57 206 628.5 0 206 58 224 628.5 0 224 59 203 628.5 0 203 60 271 628.5 0 271 61 429 628.5 0 429 62 540 628.5 0 540 63 847 628.5 0 847 64 1347 628.5 0 1347 65 18068 628.5 0 18068 66 10526 628.5 0 10526 67 25376 628.5 0 25376 68 221 628.5 0 221 69 142 628.5 0 142 70 143 628.5 0 143 71 132 628.5 0 132 72 150 628.5 0 150 73 138 628.5 0 138 74 118 628.5 0 118 75 105 628.5 0 105 76 119 628.5 0 119 77 151 628.5 0 151 78 127 628.5 0 127 79 145 628.5 0 145 80 129 628.5 0 129 81 134 628.5 0 134 82 133 628.5 0 133 83 134 628.5 0 134 84 152 628.5 0 152 85 132 628.5 0 132 86 118 628.5 0 118 87 167 628.5 0 167 88 127 628.5 0 127 89 132 628.5 0 132 90 121 628.5 0 121 91 124 628.5 0 124 92 127 628.5 0 127 93 132 628.5 0 132 94 131 628.5 0 131 95 184 628.5 0 184 96 122 628.5 0 122 97 125 628.5 0 125 98 148 628.5 0 148 99 156 628.5 0 156 100 139 628.5 0 139 101 144 628.5 0 144 102 155 628.5 0 155 103 125 628.5 0 125 104 130 628.5 0 130 105 125 628.5 0 125 106 137 628.5 0 137 107 128 628.5 0 128 108 143 628.5 0 143 109 110 628.5 0 110 110 131 628.5 0 131 111 129 628.5 0 129 112 147 628.5 0 147 113 123 628.5 0 123 114 110 628.5 0 110 115 117 628.5 0 117 116 107 628.5 0 107 117 100 628.5 0 100 118 124 628.5 0 124 119 88 628.5 0 88 120 97 628.5 0 97 121 132 628.5 0 132 122 86 628.5 0 86 123 117 628.5 0 117 124 125 628.5 0 125 125 118 628.5 0 118 126 129 628.5 0 129 127 111 628.5 0 111 128 95 628.5 0 95 129 95 628.5 0 95 130 103 628.5 0 103 131 114 628.5 0 114 132 139 628.5 0 139 133 125 628.5 0 125 134 172 628.5 0 172 135 155 628.5 0 155 136 151 628.5 0 151 137 112 628.5 0 112 138 146 628.5 0 146 139 125 628.5 0 125 140 131 628.5 0 131 141 108 628.5 0 108 142 104 628.5 0 104 143 87 628.5 0 87 144 95 628.5 0 95 145 95 628.5 0 95 146 115 628.5 0 115 147 87 628.5 0 87 148 99 628.5 0 99 149 106 628.5 0 106 150 81 628.5 0 81 151 86 628.5 0 86 152 86 628.5 0 86 153 87 628.5 0 87 154 102 628.5 0 102 155 73 628.5 0 73 156 86 628.5 0 86 157 84 628.5 0 84 158 71 628.5 0 71 159 108 628.5 0 108 160 86 628.5 0 86 161 94 628.5 0 94 162 102 628.5 0 102 163 99 628.5 0 99 164 102 628.5 0 102 165 71 628.5 0 71 166 94 628.5 0 94 167 87 628.5 0 87 168 116 628.5 0 116 169 100 628.5 0 100 170 115 628.5 0 115 171 90 628.5 0 90 172 107 628.5 0 107 173 105 628.5 0 105 174 120 628.5 0 120 175 101 628.5 0 101 176 74 628.5 0 74 177 96 628.5 0 96 178 112 628.5 0 112 179 130 628.5 0 130 180 106 628.5 0 106 181 119 628.5 0 119 182 82 628.5 0 82 183 89 628.5 0 89 184 102 628.5 0 102 185 111 628.5 0 111 186 111 628.5 0 111 187 76 628.5 0 76 188 104 628.5 0 104 189 106 628.5 0 106 190 90 628.5 0 90 191 130 628.5 0 130 192 86 628.5 0 86 193 82 628.5 0 82 194 98 628.5 0 98 195 87 628.5 0 87 196 82 628.5 0 82 197 134 628.5 0 134 198 110 628.5 0 110 199 111 628.5 0 111 200 142 628.5 0 142 201 106 628.5 0 106 202 84 628.5 0 84 203 111 628.5 0 111 204 87 628.5 0 87 205 90 628.5 0 90 206 79 628.5 0 79 207 76 628.5 0 76 208 77 628.5 0 77 209 109 628.5 0 109 210 91 628.5 0 91 211 98 628.5 0 98 212 79 628.5 0 79 213 68 628.5 0 68 214 80 628.5 0 80 215 91 628.5 0 91 216 120 628.5 0 120 217 108 628.5 0 108 218 114 628.5 0 114 219 110 628.5 0 110 220 134 628.5 0 134 221 89 628.5 0 89 222 237 628.5 0 237 223 182 628.5 0 182 224 328 628.5 0 328 225 290 628.5 0 290 226 106 628.5 0 106 227 96 628.5 0 96 228 113 628.5 0 113 229 92 628.5 0 92 230 105 628.5 0 105 231 76 628.5 0 76 232 79 628.5 0 79 233 93 628.5 0 93 234 85 628.5 0 85 235 97 628.5 0 97 236 133 628.5 0 133 237 110 628.5 0 110 238 99 628.5 0 99 239 79 628.5 0 79 240 109 628.5 0 109 241 98 628.5 0 98 242 214 628.5 0 214 243 202 628.5 0 202 244 146 628.5 0 146 245 163 628.5 0 163 246 126 628.5 0 126 247 133 628.5 0 133 248 89 628.5 0 89 249 109 628.5 0 109 250 139 628.5 0 139 251 143 628.5 0 143 252 139 628.5 0 139 253 219 628.5 0 219 254 284 628.5 0 284 255 1714 628.5 0 1714 256 498 628.5 0 498 257 1131 628.5 0 1131 258 201 628.5 0 201 259 133 628.5 0 133 260 997 628.5 0 997 261 1632 628.5 0 1632 262 553 628.5 0 553 263 259 628.5 0 259 264 126 628.5 0 126 265 121 628.5 0 121 266 171 628.5 0 171 267 97 628.5 0 97 268 77 628.5 0 77 269 102 628.5 0 102 270 272 628.5 0 272 271 290 628.5 0 290 272 393 628.5 0 393 273 99 628.5 0 99 274 81 628.5 0 81 275 91 628.5 0 91 276 85 628.5 0 85 277 99 628.5 0 99 278 119 628.5 0 119 279 113 628.5 0 113 280 86 628.5 0 86 281 64 628.5 0 64 282 69 628.5 0 69 283 53 628.5 0 53 284 91 628.5 0 91 285 67 628.5 0 67 286 69 628.5 0 69 287 76 628.5 0 76 288 66 628.5 0 66 289 68 628.5 0 68 290 79 628.5 0 79 291 89 628.5 0 89 292 120 628.5 0 120 293 126 628.5 0 126 294 223 628.5 0 223 295 68 628.5 0 68 296 87 628.5 0 87 297 66 628.5 0 66 298 84 628.5 0 84 299 147 628.5 0 147 300 107 628.5 0 107 301 113 628.5 0 113 Finished in 4.84 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 115,999 Reads with adapters: 7,599 (6.6%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 7,579 (6.5%) Total basepairs processed: 27,942,224 bp Total written (filtered): 2,082,125 bp (7.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7599 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 61 0.0 3 0 59 2 17 6157 0.0 3 6072 82 3 18 10 0.0 3 1 9 19 22 0.0 3 3 10 6 3 20 1308 0.0 3 1273 16 2 17 21 1 0.0 3 0 1 22 2 0.0 3 0 0 2 30 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 57 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 167 5 0.0 3 0 0 1 4 171 2 0.0 3 0 0 1 1 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 210 1 0.0 3 1 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 258 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 7,579 Reads with adapters: 165 (2.2%) Reads written (passing filters): 7,579 (100.0%) Total basepairs processed: 2,082,125 bp Total written (filtered): 2,081,617 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 165 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 70.3% C: 9.7% G: 17.6% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 154 118.4 0 154 4 9 29.6 0 9 5 2 7.4 0 2