This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.81 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 126,392 (4.9%) Reads that were too short: 23,284 (0.9%) Reads written (passing filters): 103,108 (4.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 24,812,325 bp (3.2%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 126392 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 729 628.5 0 729 7 712 628.5 0 712 8 30289 628.5 0 30289 9 26692 628.5 0 26692 10 110 628.5 0 110 11 104 628.5 0 104 12 97 628.5 0 97 13 76 628.5 0 76 14 83 628.5 0 83 15 88 628.5 0 88 16 88 628.5 0 88 17 100 628.5 0 100 18 99 628.5 0 99 19 93 628.5 0 93 20 79 628.5 0 79 21 73 628.5 0 73 22 101 628.5 0 101 23 88 628.5 0 88 24 114 628.5 0 114 25 154 628.5 0 154 26 178 628.5 0 178 27 88 628.5 0 88 28 100 628.5 0 100 29 117 628.5 0 117 30 85 628.5 0 85 31 77 628.5 0 77 32 55 628.5 0 55 33 74 628.5 0 74 34 108 628.5 0 108 35 110 628.5 0 110 36 182 628.5 0 182 37 94 628.5 0 94 38 108 628.5 0 108 39 86 628.5 0 86 40 74 628.5 0 74 41 67 628.5 0 67 42 76 628.5 0 76 43 97 628.5 0 97 44 78 628.5 0 78 45 71 628.5 0 71 46 84 628.5 0 84 47 89 628.5 0 89 48 80 628.5 0 80 49 92 628.5 0 92 50 67 628.5 0 67 51 68 628.5 0 68 52 92 628.5 0 92 53 78 628.5 0 78 54 98 628.5 0 98 55 78 628.5 0 78 56 61 628.5 0 61 57 84 628.5 0 84 58 93 628.5 0 93 59 64 628.5 0 64 60 63 628.5 0 63 61 84 628.5 0 84 62 92 628.5 0 92 63 93 628.5 0 93 64 85 628.5 0 85 65 126 628.5 0 126 66 103 628.5 0 103 67 193 628.5 0 193 68 89 628.5 0 89 69 210 628.5 0 210 70 125 628.5 0 125 71 206 628.5 0 206 72 87 628.5 0 87 73 81 628.5 0 81 74 73 628.5 0 73 75 111 628.5 0 111 76 144 628.5 0 144 77 260 628.5 0 260 78 433 628.5 0 433 79 139 628.5 0 139 80 729 628.5 0 729 81 309 628.5 0 309 82 326 628.5 0 326 83 153 628.5 0 153 84 97 628.5 0 97 85 98 628.5 0 98 86 120 628.5 0 120 87 102 628.5 0 102 88 93 628.5 0 93 89 132 628.5 0 132 90 120 628.5 0 120 91 105 628.5 0 105 92 116 628.5 0 116 93 156 628.5 0 156 94 163 628.5 0 163 95 185 628.5 0 185 96 1017 628.5 0 1017 97 2649 628.5 0 2649 98 3471 628.5 0 3471 99 142 628.5 0 142 100 143 628.5 0 143 101 127 628.5 0 127 102 102 628.5 0 102 103 97 628.5 0 97 104 92 628.5 0 92 105 88 628.5 0 88 106 78 628.5 0 78 107 104 628.5 0 104 108 109 628.5 0 109 109 80 628.5 0 80 110 106 628.5 0 106 111 109 628.5 0 109 112 117 628.5 0 117 113 118 628.5 0 118 114 158 628.5 0 158 115 114 628.5 0 114 116 94 628.5 0 94 117 83 628.5 0 83 118 83 628.5 0 83 119 94 628.5 0 94 120 126 628.5 0 126 121 93 628.5 0 93 122 81 628.5 0 81 123 99 628.5 0 99 124 109 628.5 0 109 125 104 628.5 0 104 126 129 628.5 0 129 127 161 628.5 0 161 128 270 628.5 0 270 129 243 628.5 0 243 130 723 628.5 0 723 131 652 628.5 0 652 132 740 628.5 0 740 133 360 628.5 0 360 134 326 628.5 0 326 135 141 628.5 0 141 136 93 628.5 0 93 137 102 628.5 0 102 138 144 628.5 0 144 139 194 628.5 0 194 140 110 628.5 0 110 141 132 628.5 0 132 142 94 628.5 0 94 143 142 628.5 0 142 144 108 628.5 0 108 145 103 628.5 0 103 146 105 628.5 0 105 147 130 628.5 0 130 148 1216 628.5 0 1216 149 1366 628.5 0 1366 150 441 628.5 0 441 151 92 628.5 0 92 152 92 628.5 0 92 153 76 628.5 0 76 154 86 628.5 0 86 155 103 628.5 0 103 156 100 628.5 0 100 157 91 628.5 0 91 158 165 628.5 0 165 159 144 628.5 0 144 160 152 628.5 0 152 161 111 628.5 0 111 162 89 628.5 0 89 163 98 628.5 0 98 164 96 628.5 0 96 165 101 628.5 0 101 166 85 628.5 0 85 167 79 628.5 0 79 168 94 628.5 0 94 169 92 628.5 0 92 170 93 628.5 0 93 171 74 628.5 0 74 172 103 628.5 0 103 173 84 628.5 0 84 174 87 628.5 0 87 175 107 628.5 0 107 176 93 628.5 0 93 177 87 628.5 0 87 178 107 628.5 0 107 179 110 628.5 0 110 180 123 628.5 0 123 181 134 628.5 0 134 182 109 628.5 0 109 183 115 628.5 0 115 184 212 628.5 0 212 185 268 628.5 0 268 186 275 628.5 0 275 187 1331 628.5 0 1331 188 4108 628.5 0 4108 189 4133 628.5 0 4133 190 1377 628.5 0 1377 191 102 628.5 0 102 192 84 628.5 0 84 193 79 628.5 0 79 194 96 628.5 0 96 195 84 628.5 0 84 196 96 628.5 0 96 197 103 628.5 0 103 198 115 628.5 0 115 199 80 628.5 0 80 200 61 628.5 0 61 201 97 628.5 0 97 202 77 628.5 0 77 203 99 628.5 0 99 204 102 628.5 0 102 205 128 628.5 0 128 206 103 628.5 0 103 207 149 628.5 0 149 208 118 628.5 0 118 209 599 628.5 0 599 210 636 628.5 0 636 211 605 628.5 0 605 212 293 628.5 0 293 213 101 628.5 0 101 214 106 628.5 0 106 215 103 628.5 0 103 216 76 628.5 0 76 217 82 628.5 0 82 218 66 628.5 0 66 219 99 628.5 0 99 220 98 628.5 0 98 221 82 628.5 0 82 222 107 628.5 0 107 223 129 628.5 0 129 224 110 628.5 0 110 225 99 628.5 0 99 226 198 628.5 0 198 227 171 628.5 0 171 228 131 628.5 0 131 229 94 628.5 0 94 230 125 628.5 0 125 231 130 628.5 0 130 232 140 628.5 0 140 233 176 628.5 0 176 234 246 628.5 0 246 235 335 628.5 0 335 236 166 628.5 0 166 237 125 628.5 0 125 238 111 628.5 0 111 239 124 628.5 0 124 240 476 628.5 0 476 241 634 628.5 0 634 242 864 628.5 0 864 243 233 628.5 0 233 244 272 628.5 0 272 245 144 628.5 0 144 246 153 628.5 0 153 247 163 628.5 0 163 248 196 628.5 0 196 249 541 628.5 0 541 250 504 628.5 0 504 251 689 628.5 0 689 252 305 628.5 0 305 253 886 628.5 0 886 254 1006 628.5 0 1006 255 755 628.5 0 755 256 459 628.5 0 459 257 142 628.5 0 142 258 208 628.5 0 208 259 201 628.5 0 201 260 483 628.5 0 483 261 339 628.5 0 339 262 483 628.5 0 483 263 162 628.5 0 162 264 105 628.5 0 105 265 193 628.5 0 193 266 212 628.5 0 212 267 231 628.5 0 231 268 210 628.5 0 210 269 237 628.5 0 237 270 395 628.5 0 395 271 361 628.5 0 361 272 531 628.5 0 531 273 506 628.5 0 506 274 596 628.5 0 596 275 433 628.5 0 433 276 73 628.5 0 73 277 58 628.5 0 58 278 63 628.5 0 63 279 93 628.5 0 93 280 92 628.5 0 92 281 117 628.5 0 117 282 115 628.5 0 115 283 176 628.5 0 176 284 178 628.5 0 178 285 155 628.5 0 155 286 73 628.5 0 73 287 70 628.5 0 70 288 57 628.5 0 57 289 75 628.5 0 75 290 56 628.5 0 56 291 61 628.5 0 61 292 99 628.5 0 99 293 98 628.5 0 98 294 113 628.5 0 113 295 101 628.5 0 101 296 86 628.5 0 86 297 82 628.5 0 82 298 86 628.5 0 86 299 118 628.5 0 118 300 84 628.5 0 84 301 158 628.5 0 158 Finished in 5.07 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 103,108 Reads with adapters: 28,051 (27.2%) Reads that were too short: 166 (0.2%) Reads written (passing filters): 27,885 (27.0%) Total basepairs processed: 24,812,325 bp Total written (filtered): 7,567,115 bp (30.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28051 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 5 2 18 38 0.0 3 0 0 13 13 12 19 453 0.0 3 0 368 39 34 12 20 26977 0.0 4 24424 1950 417 140 46 21 69 0.0 4 6 56 5 2 22 4 0.0 4 1 0 1 0 2 23 154 0.0 4 0 0 0 102 52 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 26 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 37 2 0.0 4 1 0 0 0 1 38 2 0.0 4 0 0 0 0 2 41 5 0.0 4 0 0 0 0 5 42 2 0.0 4 0 0 0 1 1 43 2 0.0 4 0 0 0 0 2 53 1 0.0 4 0 0 0 0 1 54 10 0.0 4 0 0 0 0 10 55 2 0.0 4 0 0 0 0 2 56 56 0.0 4 0 0 0 1 55 58 26 0.0 4 0 0 0 0 26 61 1 0.0 4 0 0 0 1 64 1 0.0 4 0 0 0 0 1 74 2 0.0 4 1 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 84 3 0.0 4 0 1 1 0 1 86 1 0.0 4 0 0 1 87 8 0.0 4 0 0 0 0 8 88 13 0.0 4 0 0 0 1 12 89 2 0.0 4 0 2 92 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 100 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 127 0.0 4 0 0 0 0 127 130 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 147 2 0.0 4 0 0 0 0 2 148 2 0.0 4 0 0 0 0 2 149 4 0.0 4 0 0 0 0 4 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 1 160 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 192 4 0.0 4 0 0 0 0 4 193 1 0.0 4 0 0 0 0 1 195 2 0.0 4 0 0 0 0 2 196 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 0 8 229 4 0.0 4 0 0 0 0 4 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.02 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 27,885 Reads with adapters: 7,383 (26.5%) Reads written (passing filters): 27,885 (100.0%) Total basepairs processed: 7,567,115 bp Total written (filtered): 7,271,859 bp (96.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7383 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 14.3% C: 9.4% G: 74.1% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 21 435.7 0 21 4 7 108.9 0 7 7 3 1.7 0 3 8 2 0.4 0 2 9 1603 0.1 0 190 1413 10 3501 0.0 1 327 3174 11 1285 0.0 1 58 1227 12 21 0.0 1 0 21 13 24 0.0 1 0 24 14 4 0.0 1 0 4 15 1 0.0 1 0 1 150 28 0.0 1 23 5 152 1 0.0 1 0 1 169 1 0.0 1 1 177 1 0.0 1 1 208 12 0.0 1 11 1 209 1 0.0 1 1 210 1 0.0 1 1 223 12 0.0 1 11 1 224 1 0.0 1 1 256 2 0.0 1 2 257 590 0.0 1 548 42 258 25 0.0 1 21 4 259 10 0.0 1 9 1 260 2 0.0 1 2 261 1 0.0 1 1 262 210 0.0 1 192 18 263 9 0.0 1 9 264 2 0.0 1 2 265 2 0.0 1 2