This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.26 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 40,006 (1.6%) Reads that were too short: 17,292 (0.7%) Reads written (passing filters): 22,714 (0.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 4,561,098 bp (0.6%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 40006 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 133 628.5 0 133 7 2206 628.5 0 2206 8 1931 628.5 0 1931 9 49 628.5 0 49 10 54 628.5 0 54 11 63 628.5 0 63 12 60 628.5 0 60 13 39 628.5 0 39 14 74 628.5 0 74 15 37 628.5 0 37 16 39 628.5 0 39 17 30 628.5 0 30 18 38 628.5 0 38 19 45 628.5 0 45 20 55 628.5 0 55 21 77 628.5 0 77 22 93 628.5 0 93 23 42 628.5 0 42 24 49 628.5 0 49 25 35 628.5 0 35 26 34 628.5 0 34 27 33 628.5 0 33 28 24 628.5 0 24 29 40 628.5 0 40 30 59 628.5 0 59 31 40 628.5 0 40 32 44 628.5 0 44 33 34 628.5 0 34 34 40 628.5 0 40 35 47 628.5 0 47 36 68 628.5 0 68 37 69 628.5 0 69 38 70 628.5 0 70 39 78 628.5 0 78 40 101 628.5 0 101 41 66 628.5 0 66 42 41 628.5 0 41 43 44 628.5 0 44 44 43 628.5 0 43 45 43 628.5 0 43 46 40 628.5 0 40 47 35 628.5 0 35 48 163 628.5 0 163 49 79 628.5 0 79 50 38 628.5 0 38 51 70 628.5 0 70 52 54 628.5 0 54 53 47 628.5 0 47 54 45 628.5 0 45 55 55 628.5 0 55 56 44 628.5 0 44 57 53 628.5 0 53 58 43 628.5 0 43 59 41 628.5 0 41 60 98 628.5 0 98 61 108 628.5 0 108 62 80 628.5 0 80 63 40 628.5 0 40 64 55 628.5 0 55 65 58 628.5 0 58 66 48 628.5 0 48 67 62 628.5 0 62 68 67 628.5 0 67 69 295 628.5 0 295 70 412 628.5 0 412 71 511 628.5 0 511 72 256 628.5 0 256 73 78 628.5 0 78 74 354 628.5 0 354 75 47 628.5 0 47 76 41 628.5 0 41 77 45 628.5 0 45 78 45 628.5 0 45 79 59 628.5 0 59 80 124 628.5 0 124 81 125 628.5 0 125 82 105 628.5 0 105 83 108 628.5 0 108 84 364 628.5 0 364 85 177 628.5 0 177 86 227 628.5 0 227 87 61 628.5 0 61 88 70 628.5 0 70 89 48 628.5 0 48 90 64 628.5 0 64 91 60 628.5 0 60 92 50 628.5 0 50 93 43 628.5 0 43 94 46 628.5 0 46 95 49 628.5 0 49 96 45 628.5 0 45 97 61 628.5 0 61 98 51 628.5 0 51 99 71 628.5 0 71 100 54 628.5 0 54 101 89 628.5 0 89 102 66 628.5 0 66 103 44 628.5 0 44 104 44 628.5 0 44 105 35 628.5 0 35 106 40 628.5 0 40 107 51 628.5 0 51 108 50 628.5 0 50 109 47 628.5 0 47 110 115 628.5 0 115 111 173 628.5 0 173 112 295 628.5 0 295 113 53 628.5 0 53 114 45 628.5 0 45 115 40 628.5 0 40 116 41 628.5 0 41 117 47 628.5 0 47 118 29 628.5 0 29 119 53 628.5 0 53 120 44 628.5 0 44 121 50 628.5 0 50 122 28 628.5 0 28 123 67 628.5 0 67 124 69 628.5 0 69 125 62 628.5 0 62 126 44 628.5 0 44 127 45 628.5 0 45 128 46 628.5 0 46 129 35 628.5 0 35 130 40 628.5 0 40 131 73 628.5 0 73 132 273 628.5 0 273 133 54 628.5 0 54 134 48 628.5 0 48 135 38 628.5 0 38 136 41 628.5 0 41 137 42 628.5 0 42 138 64 628.5 0 64 139 51 628.5 0 51 140 45 628.5 0 45 141 53 628.5 0 53 142 65 628.5 0 65 143 60 628.5 0 60 144 52 628.5 0 52 145 94 628.5 0 94 146 131 628.5 0 131 147 155 628.5 0 155 148 240 628.5 0 240 149 166 628.5 0 166 150 221 628.5 0 221 151 130 628.5 0 130 152 100 628.5 0 100 153 143 628.5 0 143 154 63 628.5 0 63 155 62 628.5 0 62 156 178 628.5 0 178 157 131 628.5 0 131 158 167 628.5 0 167 159 98 628.5 0 98 160 37 628.5 0 37 161 68 628.5 0 68 162 38 628.5 0 38 163 49 628.5 0 49 164 50 628.5 0 50 165 53 628.5 0 53 166 46 628.5 0 46 167 65 628.5 0 65 168 101 628.5 0 101 169 121 628.5 0 121 170 192 628.5 0 192 171 59 628.5 0 59 172 51 628.5 0 51 173 55 628.5 0 55 174 70 628.5 0 70 175 77 628.5 0 77 176 390 628.5 0 390 177 364 628.5 0 364 178 542 628.5 0 542 179 52 628.5 0 52 180 49 628.5 0 49 181 47 628.5 0 47 182 102 628.5 0 102 183 138 628.5 0 138 184 88 628.5 0 88 185 44 628.5 0 44 186 62 628.5 0 62 187 132 628.5 0 132 188 202 628.5 0 202 189 273 628.5 0 273 190 105 628.5 0 105 191 71 628.5 0 71 192 53 628.5 0 53 193 50 628.5 0 50 194 65 628.5 0 65 195 105 628.5 0 105 196 185 628.5 0 185 197 395 628.5 0 395 198 695 628.5 0 695 199 500 628.5 0 500 200 96 628.5 0 96 201 116 628.5 0 116 202 421 628.5 0 421 203 758 628.5 0 758 204 792 628.5 0 792 205 376 628.5 0 376 206 193 628.5 0 193 207 58 628.5 0 58 208 47 628.5 0 47 209 84 628.5 0 84 210 109 628.5 0 109 211 91 628.5 0 91 212 123 628.5 0 123 213 209 628.5 0 209 214 233 628.5 0 233 215 180 628.5 0 180 216 169 628.5 0 169 217 139 628.5 0 139 218 130 628.5 0 130 219 145 628.5 0 145 220 98 628.5 0 98 221 98 628.5 0 98 222 247 628.5 0 247 223 877 628.5 0 877 224 1103 628.5 0 1103 225 815 628.5 0 815 226 63 628.5 0 63 227 67 628.5 0 67 228 80 628.5 0 80 229 65 628.5 0 65 230 49 628.5 0 49 231 48 628.5 0 48 232 41 628.5 0 41 233 61 628.5 0 61 234 97 628.5 0 97 235 94 628.5 0 94 236 78 628.5 0 78 237 65 628.5 0 65 238 83 628.5 0 83 239 101 628.5 0 101 240 93 628.5 0 93 241 182 628.5 0 182 242 160 628.5 0 160 243 154 628.5 0 154 244 127 628.5 0 127 245 152 628.5 0 152 246 137 628.5 0 137 247 73 628.5 0 73 248 83 628.5 0 83 249 88 628.5 0 88 250 63 628.5 0 63 251 77 628.5 0 77 252 53 628.5 0 53 253 55 628.5 0 55 254 92 628.5 0 92 255 75 628.5 0 75 256 74 628.5 0 74 257 97 628.5 0 97 258 271 628.5 0 271 259 522 628.5 0 522 260 720 628.5 0 720 261 86 628.5 0 86 262 85 628.5 0 85 263 65 628.5 0 65 264 84 628.5 0 84 265 57 628.5 0 57 266 64 628.5 0 64 267 66 628.5 0 66 268 45 628.5 0 45 269 56 628.5 0 56 270 93 628.5 0 93 271 250 628.5 0 250 272 182 628.5 0 182 273 181 628.5 0 181 274 59 628.5 0 59 275 60 628.5 0 60 276 58 628.5 0 58 277 97 628.5 0 97 278 108 628.5 0 108 279 79 628.5 0 79 280 99 628.5 0 99 281 88 628.5 0 88 282 229 628.5 0 229 283 209 628.5 0 209 284 271 628.5 0 271 285 182 628.5 0 182 286 114 628.5 0 114 287 117 628.5 0 117 288 78 628.5 0 78 289 87 628.5 0 87 290 58 628.5 0 58 291 74 628.5 0 74 292 79 628.5 0 79 293 99 628.5 0 99 294 111 628.5 0 111 295 110 628.5 0 110 296 133 628.5 0 133 297 286 628.5 0 286 298 410 628.5 0 410 299 425 628.5 0 425 300 257 628.5 0 257 301 166 628.5 0 166 Finished in 1.01 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 22,714 Reads with adapters: 2,093 (9.2%) Reads that were too short: 13 (0.1%) Reads written (passing filters): 2,080 (9.2%) Total basepairs processed: 4,561,098 bp Total written (filtered): 560,922 bp (12.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2093 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 0 1 19 45 0.0 3 0 36 5 3 1 20 1953 0.0 4 1750 153 35 15 21 4 0.0 4 1 1 1 1 22 1 0.0 4 0 0 1 23 8 0.0 4 0 0 0 6 2 25 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 1 46 1 0.0 4 0 1 49 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 63 0.0 4 0 0 0 0 63 128 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 2,080 Reads with adapters: 421 (20.2%) Reads written (passing filters): 2,080 (100.0%) Total basepairs processed: 560,922 bp Total written (filtered): 555,695 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 421 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.3% C: 10.7% G: 81.9% T: 3.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 32.5 0 5 4 3 8.1 0 3 10 28 0.0 1 0 28 11 372 0.0 1 15 357 12 4 0.0 1 0 4 13 1 0.0 1 0 1 19 4 0.0 1 2 2 20 1 0.0 1 0 1 151 1 0.0 1 1 260 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.