This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.90 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 25,549 (1.0%) Reads that were too short: 7,914 (0.3%) Reads written (passing filters): 17,635 (0.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 3,885,703 bp (0.5%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 25549 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 129 628.5 0 129 7 2125 628.5 0 2125 8 2313 628.5 0 2313 9 50 628.5 0 50 10 39 628.5 0 39 11 80 628.5 0 80 12 37 628.5 0 37 13 41 628.5 0 41 14 61 628.5 0 61 15 44 628.5 0 44 16 53 628.5 0 53 17 39 628.5 0 39 18 27 628.5 0 27 19 50 628.5 0 50 20 41 628.5 0 41 21 45 628.5 0 45 22 55 628.5 0 55 23 55 628.5 0 55 24 32 628.5 0 32 25 40 628.5 0 40 26 30 628.5 0 30 27 39 628.5 0 39 28 47 628.5 0 47 29 49 628.5 0 49 30 54 628.5 0 54 31 47 628.5 0 47 32 44 628.5 0 44 33 43 628.5 0 43 34 44 628.5 0 44 35 27 628.5 0 27 36 44 628.5 0 44 37 43 628.5 0 43 38 32 628.5 0 32 39 38 628.5 0 38 40 46 628.5 0 46 41 51 628.5 0 51 42 43 628.5 0 43 43 54 628.5 0 54 44 34 628.5 0 34 45 47 628.5 0 47 46 42 628.5 0 42 47 30 628.5 0 30 48 64 628.5 0 64 49 71 628.5 0 71 50 53 628.5 0 53 51 42 628.5 0 42 52 38 628.5 0 38 53 42 628.5 0 42 54 48 628.5 0 48 55 64 628.5 0 64 56 70 628.5 0 70 57 59 628.5 0 59 58 43 628.5 0 43 59 34 628.5 0 34 60 68 628.5 0 68 61 42 628.5 0 42 62 46 628.5 0 46 63 48 628.5 0 48 64 35 628.5 0 35 65 51 628.5 0 51 66 46 628.5 0 46 67 58 628.5 0 58 68 63 628.5 0 63 69 261 628.5 0 261 70 516 628.5 0 516 71 672 628.5 0 672 72 306 628.5 0 306 73 84 628.5 0 84 74 352 628.5 0 352 75 53 628.5 0 53 76 43 628.5 0 43 77 33 628.5 0 33 78 45 628.5 0 45 79 48 628.5 0 48 80 70 628.5 0 70 81 72 628.5 0 72 82 82 628.5 0 82 83 52 628.5 0 52 84 329 628.5 0 329 85 176 628.5 0 176 86 251 628.5 0 251 87 32 628.5 0 32 88 45 628.5 0 45 89 45 628.5 0 45 90 39 628.5 0 39 91 37 628.5 0 37 92 33 628.5 0 33 93 44 628.5 0 44 94 70 628.5 0 70 95 39 628.5 0 39 96 35 628.5 0 35 97 46 628.5 0 46 98 33 628.5 0 33 99 56 628.5 0 56 100 41 628.5 0 41 101 44 628.5 0 44 102 49 628.5 0 49 103 26 628.5 0 26 104 32 628.5 0 32 105 31 628.5 0 31 106 35 628.5 0 35 107 54 628.5 0 54 108 44 628.5 0 44 109 39 628.5 0 39 110 85 628.5 0 85 111 146 628.5 0 146 112 230 628.5 0 230 113 48 628.5 0 48 114 46 628.5 0 46 115 35 628.5 0 35 116 29 628.5 0 29 117 43 628.5 0 43 118 26 628.5 0 26 119 29 628.5 0 29 120 48 628.5 0 48 121 65 628.5 0 65 122 27 628.5 0 27 123 28 628.5 0 28 124 53 628.5 0 53 125 54 628.5 0 54 126 40 628.5 0 40 127 25 628.5 0 25 128 49 628.5 0 49 129 41 628.5 0 41 130 31 628.5 0 31 131 69 628.5 0 69 132 230 628.5 0 230 133 42 628.5 0 42 134 43 628.5 0 43 135 36 628.5 0 36 136 26 628.5 0 26 137 36 628.5 0 36 138 49 628.5 0 49 139 42 628.5 0 42 140 33 628.5 0 33 141 39 628.5 0 39 142 35 628.5 0 35 143 36 628.5 0 36 144 40 628.5 0 40 145 56 628.5 0 56 146 54 628.5 0 54 147 70 628.5 0 70 148 168 628.5 0 168 149 89 628.5 0 89 150 139 628.5 0 139 151 45 628.5 0 45 152 31 628.5 0 31 153 48 628.5 0 48 154 36 628.5 0 36 155 41 628.5 0 41 156 82 628.5 0 82 157 54 628.5 0 54 158 81 628.5 0 81 159 67 628.5 0 67 160 33 628.5 0 33 161 79 628.5 0 79 162 36 628.5 0 36 163 27 628.5 0 27 164 29 628.5 0 29 165 36 628.5 0 36 166 44 628.5 0 44 167 38 628.5 0 38 168 91 628.5 0 91 169 80 628.5 0 80 170 160 628.5 0 160 171 49 628.5 0 49 172 55 628.5 0 55 173 39 628.5 0 39 174 47 628.5 0 47 175 42 628.5 0 42 176 122 628.5 0 122 177 131 628.5 0 131 178 107 628.5 0 107 179 35 628.5 0 35 180 53 628.5 0 53 181 41 628.5 0 41 182 110 628.5 0 110 183 119 628.5 0 119 184 62 628.5 0 62 185 50 628.5 0 50 186 53 628.5 0 53 187 67 628.5 0 67 188 78 628.5 0 78 189 75 628.5 0 75 190 42 628.5 0 42 191 42 628.5 0 42 192 46 628.5 0 46 193 38 628.5 0 38 194 35 628.5 0 35 195 48 628.5 0 48 196 81 628.5 0 81 197 130 628.5 0 130 198 156 628.5 0 156 199 194 628.5 0 194 200 45 628.5 0 45 201 32 628.5 0 32 202 118 628.5 0 118 203 243 628.5 0 243 204 225 628.5 0 225 205 138 628.5 0 138 206 78 628.5 0 78 207 39 628.5 0 39 208 51 628.5 0 51 209 47 628.5 0 47 210 57 628.5 0 57 211 47 628.5 0 47 212 43 628.5 0 43 213 58 628.5 0 58 214 66 628.5 0 66 215 82 628.5 0 82 216 59 628.5 0 59 217 47 628.5 0 47 218 54 628.5 0 54 219 52 628.5 0 52 220 65 628.5 0 65 221 42 628.5 0 42 222 74 628.5 0 74 223 226 628.5 0 226 224 359 628.5 0 359 225 279 628.5 0 279 226 43 628.5 0 43 227 50 628.5 0 50 228 64 628.5 0 64 229 38 628.5 0 38 230 42 628.5 0 42 231 39 628.5 0 39 232 45 628.5 0 45 233 51 628.5 0 51 234 47 628.5 0 47 235 62 628.5 0 62 236 62 628.5 0 62 237 44 628.5 0 44 238 46 628.5 0 46 239 62 628.5 0 62 240 57 628.5 0 57 241 72 628.5 0 72 242 68 628.5 0 68 243 55 628.5 0 55 244 75 628.5 0 75 245 79 628.5 0 79 246 63 628.5 0 63 247 55 628.5 0 55 248 45 628.5 0 45 249 63 628.5 0 63 250 62 628.5 0 62 251 50 628.5 0 50 252 31 628.5 0 31 253 42 628.5 0 42 254 62 628.5 0 62 255 57 628.5 0 57 256 48 628.5 0 48 257 56 628.5 0 56 258 147 628.5 0 147 259 262 628.5 0 262 260 425 628.5 0 425 261 84 628.5 0 84 262 37 628.5 0 37 263 42 628.5 0 42 264 41 628.5 0 41 265 35 628.5 0 35 266 43 628.5 0 43 267 38 628.5 0 38 268 44 628.5 0 44 269 46 628.5 0 46 270 53 628.5 0 53 271 113 628.5 0 113 272 86 628.5 0 86 273 92 628.5 0 92 274 42 628.5 0 42 275 46 628.5 0 46 276 50 628.5 0 50 277 57 628.5 0 57 278 64 628.5 0 64 279 61 628.5 0 61 280 60 628.5 0 60 281 58 628.5 0 58 282 99 628.5 0 99 283 119 628.5 0 119 284 115 628.5 0 115 285 94 628.5 0 94 286 57 628.5 0 57 287 87 628.5 0 87 288 51 628.5 0 51 289 51 628.5 0 51 290 42 628.5 0 42 291 53 628.5 0 53 292 35 628.5 0 35 293 48 628.5 0 48 294 67 628.5 0 67 295 47 628.5 0 47 296 53 628.5 0 53 297 112 628.5 0 112 298 170 628.5 0 170 299 154 628.5 0 154 300 87 628.5 0 87 301 63 628.5 0 63 Finished in 0.74 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 17,635 Reads with adapters: 2,064 (11.7%) Reads that were too short: 23 (0.1%) Reads written (passing filters): 2,041 (11.6%) Total basepairs processed: 3,885,703 bp Total written (filtered): 563,118 bp (14.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2064 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 22 0.0 3 0 22 17 1997 0.0 3 1945 49 3 18 6 0.0 3 1 5 20 2 0.0 3 0 0 0 2 23 1 0.0 3 0 0 0 1 29 4 0.0 3 0 0 0 4 41 5 0.0 3 0 0 0 5 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 57 1 0.0 3 0 1 64 1 0.0 3 0 0 1 81 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 13 0.0 3 0 0 1 12 235 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 2,041 Reads with adapters: 0 (0.0%) Reads written (passing filters): 2,041 (100.0%) Total basepairs processed: 563,118 bp Total written (filtered): 563,118 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 0 times.