This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.55 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 175,425 (6.8%) Reads that were too short: 62,748 (2.4%) Reads written (passing filters): 112,677 (4.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 26,766,941 bp (3.5%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 175425 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4302 628.5 0 4302 7 28377 628.5 0 28377 8 520 628.5 0 520 9 31093 628.5 0 31093 10 70 628.5 0 70 11 64 628.5 0 64 12 55 628.5 0 55 13 50 628.5 0 50 14 135 628.5 0 135 15 61 628.5 0 61 16 60 628.5 0 60 17 83 628.5 0 83 18 43 628.5 0 43 19 122 628.5 0 122 20 47 628.5 0 47 21 55 628.5 0 55 22 54 628.5 0 54 23 42 628.5 0 42 24 54 628.5 0 54 25 55 628.5 0 55 26 54 628.5 0 54 27 76 628.5 0 76 28 54 628.5 0 54 29 70 628.5 0 70 30 46 628.5 0 46 31 77 628.5 0 77 32 51 628.5 0 51 33 50 628.5 0 50 34 60 628.5 0 60 35 43 628.5 0 43 36 48 628.5 0 48 37 79 628.5 0 79 38 83 628.5 0 83 39 68 628.5 0 68 40 69 628.5 0 69 41 37 628.5 0 37 42 57 628.5 0 57 43 67 628.5 0 67 44 49 628.5 0 49 45 46 628.5 0 46 46 40 628.5 0 40 47 67 628.5 0 67 48 40 628.5 0 40 49 40 628.5 0 40 50 69 628.5 0 69 51 37 628.5 0 37 52 44 628.5 0 44 53 37 628.5 0 37 54 47 628.5 0 47 55 40 628.5 0 40 56 88 628.5 0 88 57 59 628.5 0 59 58 114 628.5 0 114 59 41 628.5 0 41 60 57 628.5 0 57 61 41 628.5 0 41 62 51 628.5 0 51 63 42 628.5 0 42 64 52 628.5 0 52 65 46 628.5 0 46 66 47 628.5 0 47 67 60 628.5 0 60 68 41 628.5 0 41 69 65 628.5 0 65 70 75 628.5 0 75 71 194 628.5 0 194 72 97 628.5 0 97 73 268 628.5 0 268 74 49 628.5 0 49 75 54 628.5 0 54 76 58 628.5 0 58 77 48 628.5 0 48 78 62 628.5 0 62 79 46 628.5 0 46 80 44 628.5 0 44 81 45 628.5 0 45 82 93 628.5 0 93 83 73 628.5 0 73 84 47 628.5 0 47 85 73 628.5 0 73 86 56 628.5 0 56 87 130 628.5 0 130 88 60 628.5 0 60 89 132 628.5 0 132 90 143 628.5 0 143 91 127 628.5 0 127 92 64 628.5 0 64 93 46 628.5 0 46 94 48 628.5 0 48 95 38 628.5 0 38 96 41 628.5 0 41 97 64 628.5 0 64 98 68 628.5 0 68 99 37 628.5 0 37 100 43 628.5 0 43 101 66 628.5 0 66 102 73 628.5 0 73 103 303 628.5 0 303 104 457 628.5 0 457 105 308 628.5 0 308 106 246 628.5 0 246 107 138 628.5 0 138 108 157 628.5 0 157 109 92 628.5 0 92 110 56 628.5 0 56 111 68 628.5 0 68 112 84 628.5 0 84 113 99 628.5 0 99 114 67 628.5 0 67 115 63 628.5 0 63 116 95 628.5 0 95 117 48 628.5 0 48 118 75 628.5 0 75 119 72 628.5 0 72 120 64 628.5 0 64 121 106 628.5 0 106 122 72 628.5 0 72 123 124 628.5 0 124 124 82 628.5 0 82 125 61 628.5 0 61 126 91 628.5 0 91 127 182 628.5 0 182 128 164 628.5 0 164 129 372 628.5 0 372 130 74 628.5 0 74 131 113 628.5 0 113 132 133 628.5 0 133 133 134 628.5 0 134 134 195 628.5 0 195 135 122 628.5 0 122 136 152 628.5 0 152 137 159 628.5 0 159 138 220 628.5 0 220 139 218 628.5 0 218 140 576 628.5 0 576 141 391 628.5 0 391 142 451 628.5 0 451 143 288 628.5 0 288 144 361 628.5 0 361 145 484 628.5 0 484 146 4572 628.5 0 4572 147 3425 628.5 0 3425 148 8775 628.5 0 8775 149 393 628.5 0 393 150 3437 628.5 0 3437 151 1798 628.5 0 1798 152 4807 628.5 0 4807 153 83 628.5 0 83 154 100 628.5 0 100 155 141 628.5 0 141 156 1023 628.5 0 1023 157 243 628.5 0 243 158 625 628.5 0 625 159 127 628.5 0 127 160 248 628.5 0 248 161 286 628.5 0 286 162 228 628.5 0 228 163 127 628.5 0 127 164 88 628.5 0 88 165 83 628.5 0 83 166 124 628.5 0 124 167 78 628.5 0 78 168 135 628.5 0 135 169 58 628.5 0 58 170 76 628.5 0 76 171 38 628.5 0 38 172 51 628.5 0 51 173 48 628.5 0 48 174 52 628.5 0 52 175 197 628.5 0 197 176 233 628.5 0 233 177 149 628.5 0 149 178 66 628.5 0 66 179 72 628.5 0 72 180 107 628.5 0 107 181 215 628.5 0 215 182 217 628.5 0 217 183 230 628.5 0 230 184 169 628.5 0 169 185 176 628.5 0 176 186 255 628.5 0 255 187 153 628.5 0 153 188 159 628.5 0 159 189 82 628.5 0 82 190 63 628.5 0 63 191 61 628.5 0 61 192 49 628.5 0 49 193 53 628.5 0 53 194 69 628.5 0 69 195 70 628.5 0 70 196 74 628.5 0 74 197 48 628.5 0 48 198 47 628.5 0 47 199 74 628.5 0 74 200 82 628.5 0 82 201 68 628.5 0 68 202 82 628.5 0 82 203 115 628.5 0 115 204 155 628.5 0 155 205 144 628.5 0 144 206 195 628.5 0 195 207 128 628.5 0 128 208 232 628.5 0 232 209 223 628.5 0 223 210 156 628.5 0 156 211 148 628.5 0 148 212 492 628.5 0 492 213 196 628.5 0 196 214 246 628.5 0 246 215 106 628.5 0 106 216 67 628.5 0 67 217 53 628.5 0 53 218 101 628.5 0 101 219 171 628.5 0 171 220 362 628.5 0 362 221 255 628.5 0 255 222 256 628.5 0 256 223 136 628.5 0 136 224 371 628.5 0 371 225 125 628.5 0 125 226 283 628.5 0 283 227 114 628.5 0 114 228 116 628.5 0 116 229 115 628.5 0 115 230 125 628.5 0 125 231 110 628.5 0 110 232 119 628.5 0 119 233 110 628.5 0 110 234 136 628.5 0 136 235 146 628.5 0 146 236 232 628.5 0 232 237 174 628.5 0 174 238 171 628.5 0 171 239 134 628.5 0 134 240 94 628.5 0 94 241 136 628.5 0 136 242 124 628.5 0 124 243 188 628.5 0 188 244 209 628.5 0 209 245 184 628.5 0 184 246 143 628.5 0 143 247 141 628.5 0 141 248 120 628.5 0 120 249 177 628.5 0 177 250 210 628.5 0 210 251 253 628.5 0 253 252 340 628.5 0 340 253 417 628.5 0 417 254 809 628.5 0 809 255 1505 628.5 0 1505 256 12261 628.5 0 12261 257 11956 628.5 0 11956 258 15888 628.5 0 15888 259 3005 628.5 0 3005 260 177 628.5 0 177 261 225 628.5 0 225 262 281 628.5 0 281 263 295 628.5 0 295 264 230 628.5 0 230 265 152 628.5 0 152 266 154 628.5 0 154 267 217 628.5 0 217 268 315 628.5 0 315 269 382 628.5 0 382 270 129 628.5 0 129 271 81 628.5 0 81 272 95 628.5 0 95 273 116 628.5 0 116 274 144 628.5 0 144 275 141 628.5 0 141 276 79 628.5 0 79 277 100 628.5 0 100 278 106 628.5 0 106 279 177 628.5 0 177 280 449 628.5 0 449 281 508 628.5 0 508 282 555 628.5 0 555 283 278 628.5 0 278 284 289 628.5 0 289 285 303 628.5 0 303 286 174 628.5 0 174 287 127 628.5 0 127 288 112 628.5 0 112 289 138 628.5 0 138 290 104 628.5 0 104 291 67 628.5 0 67 292 77 628.5 0 77 293 92 628.5 0 92 294 136 628.5 0 136 295 160 628.5 0 160 296 204 628.5 0 204 297 131 628.5 0 131 298 100 628.5 0 100 299 86 628.5 0 86 300 116 628.5 0 116 301 186 628.5 0 186 Finished in 5.35 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 112,677 Reads with adapters: 33,859 (30.0%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 33,821 (30.0%) Total basepairs processed: 26,766,941 bp Total written (filtered): 9,069,679 bp (33.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33859 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 4 2 18 17 0.0 3 0 0 11 6 19 479 0.0 3 0 362 72 29 16 20 31176 0.0 4 28670 1943 419 101 43 21 72 0.0 4 9 55 5 2 1 22 11 0.0 4 0 1 8 2 23 217 0.0 4 127 13 4 51 22 24 2 0.0 4 2 26 1 0.0 4 0 0 0 1 28 7 0.0 4 0 0 0 0 7 29 1 0.0 4 0 1 30 1 0.0 4 1 31 1 0.0 4 0 0 0 0 1 34 9 0.0 4 1 0 0 0 8 36 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 57 1 0.0 4 1 60 3 0.0 4 0 0 0 0 3 61 4 0.0 4 0 0 0 0 4 62 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 7 0.0 4 0 0 0 0 7 126 46 0.0 4 0 0 0 0 46 127 1749 0.0 4 0 0 0 0 1749 128 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 130 7 0.0 4 0 0 0 1 6 132 1 0.0 4 0 0 1 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 1 165 1 0.0 4 0 0 0 1 192 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.16 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 33,821 Reads with adapters: 1,704 (5.0%) Reads written (passing filters): 33,821 (100.0%) Total basepairs processed: 9,069,679 bp Total written (filtered): 9,000,730 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1704 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.1% C: 15.0% G: 60.7% T: 11.6% none/other: 1.6% Overview of removed sequences length count expect max.err error counts 3 249 528.5 0 249 4 40 132.1 0 40 9 20 0.1 0 5 15 10 50 0.0 1 1 49 11 455 0.0 1 11 444 12 655 0.0 1 14 641 13 30 0.0 1 1 29 14 1 0.0 1 0 1 125 1 0.0 1 0 1 152 7 0.0 1 4 3 171 2 0.0 1 2 210 3 0.0 1 2 1 264 1 0.0 1 1 267 2 0.0 1 2 269 3 0.0 1 3 270 2 0.0 1 2 271 95 0.0 1 92 3 272 16 0.0 1 14 2 273 40 0.0 1 39 1 274 30 0.0 1 29 1 275 2 0.0 1 2