This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.82 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 82,901 (3.2%) Reads that were too short: 23,326 (0.9%) Reads written (passing filters): 59,575 (2.3%) Total basepairs processed: 774,927,510 bp Total written (filtered): 10,152,640 bp (1.3%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 82901 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 413 628.5 0 413 7 4521 628.5 0 4521 8 303 628.5 0 303 9 4246 628.5 0 4246 10 40 628.5 0 40 11 29 628.5 0 29 12 31 628.5 0 31 13 37 628.5 0 37 14 36 628.5 0 36 15 65 628.5 0 65 16 28 628.5 0 28 17 32 628.5 0 32 18 31 628.5 0 31 19 30 628.5 0 30 20 31 628.5 0 31 21 47 628.5 0 47 22 43 628.5 0 43 23 44 628.5 0 44 24 39 628.5 0 39 25 36 628.5 0 36 26 19 628.5 0 19 27 44 628.5 0 44 28 48 628.5 0 48 29 28 628.5 0 28 30 45 628.5 0 45 31 32 628.5 0 32 32 35 628.5 0 35 33 32 628.5 0 32 34 25 628.5 0 25 35 77 628.5 0 77 36 42 628.5 0 42 37 28 628.5 0 28 38 67 628.5 0 67 39 46 628.5 0 46 40 194 628.5 0 194 41 106 628.5 0 106 42 167 628.5 0 167 43 32 628.5 0 32 44 37 628.5 0 37 45 35 628.5 0 35 46 35 628.5 0 35 47 49 628.5 0 49 48 27 628.5 0 27 49 38 628.5 0 38 50 38 628.5 0 38 51 43 628.5 0 43 52 40 628.5 0 40 53 50 628.5 0 50 54 34 628.5 0 34 55 248 628.5 0 248 56 135 628.5 0 135 57 323 628.5 0 323 58 42 628.5 0 42 59 53 628.5 0 53 60 81 628.5 0 81 61 46 628.5 0 46 62 45 628.5 0 45 63 42 628.5 0 42 64 59 628.5 0 59 65 47 628.5 0 47 66 90 628.5 0 90 67 53 628.5 0 53 68 83 628.5 0 83 69 37 628.5 0 37 70 41 628.5 0 41 71 68 628.5 0 68 72 47 628.5 0 47 73 65 628.5 0 65 74 63 628.5 0 63 75 78 628.5 0 78 76 44 628.5 0 44 77 44 628.5 0 44 78 46 628.5 0 46 79 47 628.5 0 47 80 23 628.5 0 23 81 24 628.5 0 24 82 44 628.5 0 44 83 41 628.5 0 41 84 90 628.5 0 90 85 34 628.5 0 34 86 30 628.5 0 30 87 43 628.5 0 43 88 43 628.5 0 43 89 64 628.5 0 64 90 102 628.5 0 102 91 69 628.5 0 69 92 47 628.5 0 47 93 50 628.5 0 50 94 39 628.5 0 39 95 61 628.5 0 61 96 38 628.5 0 38 97 39 628.5 0 39 98 26 628.5 0 26 99 23 628.5 0 23 100 19 628.5 0 19 101 55 628.5 0 55 102 74 628.5 0 74 103 99 628.5 0 99 104 79 628.5 0 79 105 304 628.5 0 304 106 508 628.5 0 508 107 293 628.5 0 293 108 24 628.5 0 24 109 37 628.5 0 37 110 49 628.5 0 49 111 56 628.5 0 56 112 91 628.5 0 91 113 102 628.5 0 102 114 109 628.5 0 109 115 262 628.5 0 262 116 82 628.5 0 82 117 92 628.5 0 92 118 71 628.5 0 71 119 38 628.5 0 38 120 43 628.5 0 43 121 62 628.5 0 62 122 50 628.5 0 50 123 46 628.5 0 46 124 52 628.5 0 52 125 67 628.5 0 67 126 117 628.5 0 117 127 232 628.5 0 232 128 81 628.5 0 81 129 144 628.5 0 144 130 158 628.5 0 158 131 82 628.5 0 82 132 159 628.5 0 159 133 106 628.5 0 106 134 161 628.5 0 161 135 84 628.5 0 84 136 88 628.5 0 88 137 98 628.5 0 98 138 82 628.5 0 82 139 78 628.5 0 78 140 114 628.5 0 114 141 105 628.5 0 105 142 159 628.5 0 159 143 143 628.5 0 143 144 229 628.5 0 229 145 224 628.5 0 224 146 310 628.5 0 310 147 4358 628.5 0 4358 148 7329 628.5 0 7329 149 4796 628.5 0 4796 150 821 628.5 0 821 151 115 628.5 0 115 152 258 628.5 0 258 153 250 628.5 0 250 154 592 628.5 0 592 155 125 628.5 0 125 156 166 628.5 0 166 157 111 628.5 0 111 158 93 628.5 0 93 159 108 628.5 0 108 160 63 628.5 0 63 161 58 628.5 0 58 162 84 628.5 0 84 163 61 628.5 0 61 164 113 628.5 0 113 165 45 628.5 0 45 166 49 628.5 0 49 167 62 628.5 0 62 168 253 628.5 0 253 169 112 628.5 0 112 170 127 628.5 0 127 171 83 628.5 0 83 172 307 628.5 0 307 173 319 628.5 0 319 174 358 628.5 0 358 175 336 628.5 0 336 176 90 628.5 0 90 177 111 628.5 0 111 178 141 628.5 0 141 179 98 628.5 0 98 180 64 628.5 0 64 181 60 628.5 0 60 182 112 628.5 0 112 183 138 628.5 0 138 184 216 628.5 0 216 185 136 628.5 0 136 186 200 628.5 0 200 187 311 628.5 0 311 188 3487 628.5 0 3487 189 1103 628.5 0 1103 190 3225 628.5 0 3225 191 118 628.5 0 118 192 53 628.5 0 53 193 74 628.5 0 74 194 81 628.5 0 81 195 118 628.5 0 118 196 211 628.5 0 211 197 211 628.5 0 211 198 247 628.5 0 247 199 370 628.5 0 370 200 635 628.5 0 635 201 6263 628.5 0 6263 202 2147 628.5 0 2147 203 2958 628.5 0 2958 204 855 628.5 0 855 205 134 628.5 0 134 206 115 628.5 0 115 207 52 628.5 0 52 208 46 628.5 0 46 209 31 628.5 0 31 210 60 628.5 0 60 211 40 628.5 0 40 212 34 628.5 0 34 213 49 628.5 0 49 214 87 628.5 0 87 215 141 628.5 0 141 216 94 628.5 0 94 217 59 628.5 0 59 218 25 628.5 0 25 219 36 628.5 0 36 220 56 628.5 0 56 221 52 628.5 0 52 222 54 628.5 0 54 223 60 628.5 0 60 224 93 628.5 0 93 225 126 628.5 0 126 226 130 628.5 0 130 227 159 628.5 0 159 228 293 628.5 0 293 229 228 628.5 0 228 230 274 628.5 0 274 231 133 628.5 0 133 232 62 628.5 0 62 233 54 628.5 0 54 234 73 628.5 0 73 235 60 628.5 0 60 236 72 628.5 0 72 237 45 628.5 0 45 238 47 628.5 0 47 239 98 628.5 0 98 240 79 628.5 0 79 241 171 628.5 0 171 242 52 628.5 0 52 243 105 628.5 0 105 244 100 628.5 0 100 245 118 628.5 0 118 246 78 628.5 0 78 247 81 628.5 0 81 248 97 628.5 0 97 249 149 628.5 0 149 250 275 628.5 0 275 251 102 628.5 0 102 252 162 628.5 0 162 253 101 628.5 0 101 254 79 628.5 0 79 255 40 628.5 0 40 256 50 628.5 0 50 257 60 628.5 0 60 258 95 628.5 0 95 259 103 628.5 0 103 260 164 628.5 0 164 261 136 628.5 0 136 262 125 628.5 0 125 263 129 628.5 0 129 264 78 628.5 0 78 265 85 628.5 0 85 266 94 628.5 0 94 267 94 628.5 0 94 268 105 628.5 0 105 269 817 628.5 0 817 270 419 628.5 0 419 271 748 628.5 0 748 272 127 628.5 0 127 273 76 628.5 0 76 274 127 628.5 0 127 275 228 628.5 0 228 276 299 628.5 0 299 277 145 628.5 0 145 278 157 628.5 0 157 279 90 628.5 0 90 280 269 628.5 0 269 281 284 628.5 0 284 282 552 628.5 0 552 283 288 628.5 0 288 284 374 628.5 0 374 285 141 628.5 0 141 286 118 628.5 0 118 287 144 628.5 0 144 288 223 628.5 0 223 289 166 628.5 0 166 290 128 628.5 0 128 291 572 628.5 0 572 292 701 628.5 0 701 293 974 628.5 0 974 294 840 628.5 0 840 295 577 628.5 0 577 296 719 628.5 0 719 297 231 628.5 0 231 298 64 628.5 0 64 299 135 628.5 0 135 300 147 628.5 0 147 301 207 628.5 0 207 Finished in 2.34 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 59,575 Reads with adapters: 4,803 (8.1%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 4,772 (8.0%) Total basepairs processed: 10,152,640 bp Total written (filtered): 1,276,575 bp (12.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4803 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 5 0.0 3 0 0 2 3 19 93 0.0 3 0 70 12 8 3 20 4417 0.0 4 3989 320 71 29 8 21 10 0.0 4 2 7 1 22 9 0.0 4 3 2 3 0 1 23 29 0.0 4 2 2 1 12 12 48 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 74 2 0.0 4 0 0 0 0 2 79 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 89 7 0.0 4 0 0 0 0 7 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 1 94 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 1 121 1 0.0 4 0 0 0 0 1 127 199 0.0 4 0 0 0 0 199 128 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 144 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 1 159 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 1 0 1 233 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 4,772 Reads with adapters: 1,235 (25.9%) Reads written (passing filters): 4,772 (100.0%) Total basepairs processed: 1,276,575 bp Total written (filtered): 1,261,317 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1235 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 9.9% G: 85.7% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 74.6 0 11 4 2 18.6 0 2 8 1 0.1 0 1 9 407 0.0 0 39 368 10 694 0.0 1 49 645 11 97 0.0 1 4 93 12 5 0.0 1 0 5 13 3 0.0 1 0 3 150 1 0.0 1 0 1 170 1 0.0 1 1 208 3 0.0 1 3 223 2 0.0 1 2 256 6 0.0 1 5 1 257 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.