This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.61 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 61,879 (2.4%) Reads that were too short: 12,983 (0.5%) Reads written (passing filters): 48,896 (1.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 8,471,350 bp (1.1%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 61879 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 393 628.5 0 393 7 4258 628.5 0 4258 8 247 628.5 0 247 9 5191 628.5 0 5191 10 25 628.5 0 25 11 18 628.5 0 18 12 17 628.5 0 17 13 16 628.5 0 16 14 11 628.5 0 11 15 22 628.5 0 22 16 17 628.5 0 17 17 10 628.5 0 10 18 22 628.5 0 22 19 18 628.5 0 18 20 20 628.5 0 20 21 17 628.5 0 17 22 15 628.5 0 15 23 13 628.5 0 13 24 20 628.5 0 20 25 18 628.5 0 18 26 21 628.5 0 21 27 23 628.5 0 23 28 18 628.5 0 18 29 16 628.5 0 16 30 27 628.5 0 27 31 21 628.5 0 21 32 22 628.5 0 22 33 18 628.5 0 18 34 23 628.5 0 23 35 23 628.5 0 23 36 27 628.5 0 27 37 19 628.5 0 19 38 24 628.5 0 24 39 14 628.5 0 14 40 29 628.5 0 29 41 18 628.5 0 18 42 18 628.5 0 18 43 18 628.5 0 18 44 17 628.5 0 17 45 17 628.5 0 17 46 32 628.5 0 32 47 31 628.5 0 31 48 9 628.5 0 9 49 21 628.5 0 21 50 28 628.5 0 28 51 25 628.5 0 25 52 9 628.5 0 9 53 15 628.5 0 15 54 18 628.5 0 18 55 44 628.5 0 44 56 34 628.5 0 34 57 53 628.5 0 53 58 17 628.5 0 17 59 23 628.5 0 23 60 19 628.5 0 19 61 21 628.5 0 21 62 17 628.5 0 17 63 25 628.5 0 25 64 17 628.5 0 17 65 27 628.5 0 27 66 24 628.5 0 24 67 18 628.5 0 18 68 18 628.5 0 18 69 20 628.5 0 20 70 21 628.5 0 21 71 21 628.5 0 21 72 19 628.5 0 19 73 22 628.5 0 22 74 37 628.5 0 37 75 31 628.5 0 31 76 36 628.5 0 36 77 15 628.5 0 15 78 20 628.5 0 20 79 35 628.5 0 35 80 15 628.5 0 15 81 18 628.5 0 18 82 23 628.5 0 23 83 16 628.5 0 16 84 38 628.5 0 38 85 26 628.5 0 26 86 30 628.5 0 30 87 28 628.5 0 28 88 24 628.5 0 24 89 30 628.5 0 30 90 32 628.5 0 32 91 14 628.5 0 14 92 10 628.5 0 10 93 25 628.5 0 25 94 37 628.5 0 37 95 31 628.5 0 31 96 21 628.5 0 21 97 24 628.5 0 24 98 22 628.5 0 22 99 25 628.5 0 25 100 12 628.5 0 12 101 29 628.5 0 29 102 28 628.5 0 28 103 63 628.5 0 63 104 34 628.5 0 34 105 123 628.5 0 123 106 173 628.5 0 173 107 111 628.5 0 111 108 12 628.5 0 12 109 31 628.5 0 31 110 27 628.5 0 27 111 35 628.5 0 35 112 30 628.5 0 30 113 49 628.5 0 49 114 62 628.5 0 62 115 213 628.5 0 213 116 24 628.5 0 24 117 24 628.5 0 24 118 36 628.5 0 36 119 21 628.5 0 21 120 27 628.5 0 27 121 40 628.5 0 40 122 38 628.5 0 38 123 24 628.5 0 24 124 28 628.5 0 28 125 29 628.5 0 29 126 76 628.5 0 76 127 117 628.5 0 117 128 78 628.5 0 78 129 110 628.5 0 110 130 101 628.5 0 101 131 57 628.5 0 57 132 88 628.5 0 88 133 83 628.5 0 83 134 140 628.5 0 140 135 43 628.5 0 43 136 71 628.5 0 71 137 94 628.5 0 94 138 81 628.5 0 81 139 50 628.5 0 50 140 85 628.5 0 85 141 85 628.5 0 85 142 124 628.5 0 124 143 101 628.5 0 101 144 170 628.5 0 170 145 173 628.5 0 173 146 212 628.5 0 212 147 4675 628.5 0 4675 148 7970 628.5 0 7970 149 4846 628.5 0 4846 150 811 628.5 0 811 151 74 628.5 0 74 152 138 628.5 0 138 153 77 628.5 0 77 154 123 628.5 0 123 155 39 628.5 0 39 156 122 628.5 0 122 157 29 628.5 0 29 158 27 628.5 0 27 159 43 628.5 0 43 160 25 628.5 0 25 161 30 628.5 0 30 162 52 628.5 0 52 163 30 628.5 0 30 164 27 628.5 0 27 165 27 628.5 0 27 166 24 628.5 0 24 167 35 628.5 0 35 168 163 628.5 0 163 169 47 628.5 0 47 170 86 628.5 0 86 171 65 628.5 0 65 172 156 628.5 0 156 173 111 628.5 0 111 174 200 628.5 0 200 175 82 628.5 0 82 176 23 628.5 0 23 177 32 628.5 0 32 178 39 628.5 0 39 179 33 628.5 0 33 180 35 628.5 0 35 181 25 628.5 0 25 182 49 628.5 0 49 183 56 628.5 0 56 184 83 628.5 0 83 185 41 628.5 0 41 186 94 628.5 0 94 187 142 628.5 0 142 188 1336 628.5 0 1336 189 450 628.5 0 450 190 1589 628.5 0 1589 191 47 628.5 0 47 192 54 628.5 0 54 193 40 628.5 0 40 194 43 628.5 0 43 195 47 628.5 0 47 196 63 628.5 0 63 197 126 628.5 0 126 198 118 628.5 0 118 199 302 628.5 0 302 200 559 628.5 0 559 201 7782 628.5 0 7782 202 2249 628.5 0 2249 203 4396 628.5 0 4396 204 848 628.5 0 848 205 29 628.5 0 29 206 36 628.5 0 36 207 24 628.5 0 24 208 34 628.5 0 34 209 25 628.5 0 25 210 20 628.5 0 20 211 25 628.5 0 25 212 22 628.5 0 22 213 21 628.5 0 21 214 17 628.5 0 17 215 42 628.5 0 42 216 24 628.5 0 24 217 37 628.5 0 37 218 19 628.5 0 19 219 19 628.5 0 19 220 13 628.5 0 13 221 19 628.5 0 19 222 27 628.5 0 27 223 19 628.5 0 19 224 30 628.5 0 30 225 65 628.5 0 65 226 46 628.5 0 46 227 88 628.5 0 88 228 143 628.5 0 143 229 111 628.5 0 111 230 130 628.5 0 130 231 30 628.5 0 30 232 30 628.5 0 30 233 17 628.5 0 17 234 27 628.5 0 27 235 28 628.5 0 28 236 34 628.5 0 34 237 26 628.5 0 26 238 23 628.5 0 23 239 24 628.5 0 24 240 35 628.5 0 35 241 32 628.5 0 32 242 44 628.5 0 44 243 33 628.5 0 33 244 36 628.5 0 36 245 43 628.5 0 43 246 13 628.5 0 13 247 28 628.5 0 28 248 37 628.5 0 37 249 41 628.5 0 41 250 65 628.5 0 65 251 29 628.5 0 29 252 57 628.5 0 57 253 33 628.5 0 33 254 35 628.5 0 35 255 13 628.5 0 13 256 14 628.5 0 14 257 33 628.5 0 33 258 30 628.5 0 30 259 39 628.5 0 39 260 41 628.5 0 41 261 40 628.5 0 40 262 47 628.5 0 47 263 27 628.5 0 27 264 26 628.5 0 26 265 27 628.5 0 27 266 27 628.5 0 27 267 45 628.5 0 45 268 48 628.5 0 48 269 221 628.5 0 221 270 100 628.5 0 100 271 182 628.5 0 182 272 52 628.5 0 52 273 29 628.5 0 29 274 53 628.5 0 53 275 86 628.5 0 86 276 117 628.5 0 117 277 48 628.5 0 48 278 46 628.5 0 46 279 33 628.5 0 33 280 85 628.5 0 85 281 63 628.5 0 63 282 186 628.5 0 186 283 110 628.5 0 110 284 131 628.5 0 131 285 34 628.5 0 34 286 37 628.5 0 37 287 44 628.5 0 44 288 73 628.5 0 73 289 52 628.5 0 52 290 50 628.5 0 50 291 167 628.5 0 167 292 224 628.5 0 224 293 213 628.5 0 213 294 218 628.5 0 218 295 102 628.5 0 102 296 122 628.5 0 122 297 67 628.5 0 67 298 17 628.5 0 17 299 67 628.5 0 67 300 47 628.5 0 47 301 72 628.5 0 72 Finished in 1.76 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 48,896 Reads with adapters: 4,804 (9.8%) Reads that were too short: 226 (0.5%) Reads written (passing filters): 4,578 (9.4%) Total basepairs processed: 8,471,350 bp Total written (filtered): 1,255,826 bp (14.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4804 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 30 0.0 3 0 30 17 4437 0.0 3 4353 82 1 1 18 19 0.0 3 2 17 19 23 0.0 3 2 0 2 19 20 7 0.0 3 1 0 0 6 22 3 0.0 3 0 0 0 3 24 1 0.0 3 1 34 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 45 43 0.0 3 0 0 0 43 47 1 0.0 3 1 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 83 2 0.0 3 2 86 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 131 8 0.0 3 0 0 0 8 138 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 213 1 0.0 3 1 216 4 0.0 3 0 0 0 4 217 197 0.0 3 0 0 6 191 233 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 4,578 Reads with adapters: 4 (0.1%) Reads written (passing filters): 4,578 (100.0%) Total basepairs processed: 1,255,826 bp Total written (filtered): 1,255,811 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.0% C: 50.0% G: 25.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 71.5 0 2 4 1 17.9 0 1 5 1 4.5 0 1