This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.34 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 189,018 (7.3%) Reads that were too short: 43,111 (1.7%) Reads written (passing filters): 145,907 (5.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 39,098,980 bp (5.0%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 189018 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2180 628.5 0 2180 7 56499 628.5 0 56499 8 1457 628.5 0 1457 9 56943 628.5 0 56943 10 72 628.5 0 72 11 36 628.5 0 36 12 26 628.5 0 26 13 35 628.5 0 35 14 21 628.5 0 21 15 27 628.5 0 27 16 25 628.5 0 25 17 35 628.5 0 35 18 23 628.5 0 23 19 17 628.5 0 17 20 22 628.5 0 22 21 12 628.5 0 12 22 28 628.5 0 28 23 38 628.5 0 38 24 25 628.5 0 25 25 38 628.5 0 38 26 24 628.5 0 24 27 32 628.5 0 32 28 46 628.5 0 46 29 13 628.5 0 13 30 23 628.5 0 23 31 20 628.5 0 20 32 41 628.5 0 41 33 34 628.5 0 34 34 32 628.5 0 32 35 24 628.5 0 24 36 15 628.5 0 15 37 22 628.5 0 22 38 33 628.5 0 33 39 30 628.5 0 30 40 27 628.5 0 27 41 124 628.5 0 124 42 425 628.5 0 425 43 847 628.5 0 847 44 46 628.5 0 46 45 76 628.5 0 76 46 34 628.5 0 34 47 28 628.5 0 28 48 26 628.5 0 26 49 27 628.5 0 27 50 24 628.5 0 24 51 46 628.5 0 46 52 36 628.5 0 36 53 28 628.5 0 28 54 20 628.5 0 20 55 20 628.5 0 20 56 16 628.5 0 16 57 52 628.5 0 52 58 54 628.5 0 54 59 43 628.5 0 43 60 29 628.5 0 29 61 34 628.5 0 34 62 37 628.5 0 37 63 37 628.5 0 37 64 23 628.5 0 23 65 34 628.5 0 34 66 61 628.5 0 61 67 119 628.5 0 119 68 31 628.5 0 31 69 45 628.5 0 45 70 47 628.5 0 47 71 37 628.5 0 37 72 71 628.5 0 71 73 72 628.5 0 72 74 79 628.5 0 79 75 62 628.5 0 62 76 92 628.5 0 92 77 56 628.5 0 56 78 53 628.5 0 53 79 53 628.5 0 53 80 62 628.5 0 62 81 44 628.5 0 44 82 58 628.5 0 58 83 42 628.5 0 42 84 25 628.5 0 25 85 27 628.5 0 27 86 16 628.5 0 16 87 24 628.5 0 24 88 16 628.5 0 16 89 27 628.5 0 27 90 21 628.5 0 21 91 40 628.5 0 40 92 45 628.5 0 45 93 29 628.5 0 29 94 28 628.5 0 28 95 45 628.5 0 45 96 31 628.5 0 31 97 59 628.5 0 59 98 50 628.5 0 50 99 41 628.5 0 41 100 49 628.5 0 49 101 90 628.5 0 90 102 91 628.5 0 91 103 110 628.5 0 110 104 162 628.5 0 162 105 211 628.5 0 211 106 203 628.5 0 203 107 166 628.5 0 166 108 130 628.5 0 130 109 106 628.5 0 106 110 118 628.5 0 118 111 104 628.5 0 104 112 112 628.5 0 112 113 117 628.5 0 117 114 130 628.5 0 130 115 93 628.5 0 93 116 132 628.5 0 132 117 102 628.5 0 102 118 136 628.5 0 136 119 151 628.5 0 151 120 170 628.5 0 170 121 103 628.5 0 103 122 136 628.5 0 136 123 124 628.5 0 124 124 190 628.5 0 190 125 209 628.5 0 209 126 188 628.5 0 188 127 129 628.5 0 129 128 162 628.5 0 162 129 112 628.5 0 112 130 143 628.5 0 143 131 231 628.5 0 231 132 241 628.5 0 241 133 1703 628.5 0 1703 134 1112 628.5 0 1112 135 2856 628.5 0 2856 136 130 628.5 0 130 137 129 628.5 0 129 138 93 628.5 0 93 139 138 628.5 0 138 140 128 628.5 0 128 141 92 628.5 0 92 142 140 628.5 0 140 143 148 628.5 0 148 144 190 628.5 0 190 145 158 628.5 0 158 146 143 628.5 0 143 147 194 628.5 0 194 148 114 628.5 0 114 149 101 628.5 0 101 150 92 628.5 0 92 151 85 628.5 0 85 152 103 628.5 0 103 153 78 628.5 0 78 154 110 628.5 0 110 155 123 628.5 0 123 156 141 628.5 0 141 157 192 628.5 0 192 158 210 628.5 0 210 159 380 628.5 0 380 160 490 628.5 0 490 161 324 628.5 0 324 162 883 628.5 0 883 163 659 628.5 0 659 164 1313 628.5 0 1313 165 268 628.5 0 268 166 247 628.5 0 247 167 338 628.5 0 338 168 209 628.5 0 209 169 213 628.5 0 213 170 205 628.5 0 205 171 187 628.5 0 187 172 241 628.5 0 241 173 191 628.5 0 191 174 102 628.5 0 102 175 121 628.5 0 121 176 92 628.5 0 92 177 124 628.5 0 124 178 188 628.5 0 188 179 198 628.5 0 198 180 210 628.5 0 210 181 171 628.5 0 171 182 207 628.5 0 207 183 302 628.5 0 302 184 241 628.5 0 241 185 308 628.5 0 308 186 165 628.5 0 165 187 221 628.5 0 221 188 123 628.5 0 123 189 191 628.5 0 191 190 204 628.5 0 204 191 157 628.5 0 157 192 173 628.5 0 173 193 155 628.5 0 155 194 136 628.5 0 136 195 130 628.5 0 130 196 118 628.5 0 118 197 89 628.5 0 89 198 83 628.5 0 83 199 134 628.5 0 134 200 139 628.5 0 139 201 174 628.5 0 174 202 143 628.5 0 143 203 162 628.5 0 162 204 127 628.5 0 127 205 157 628.5 0 157 206 193 628.5 0 193 207 278 628.5 0 278 208 289 628.5 0 289 209 211 628.5 0 211 210 215 628.5 0 215 211 440 628.5 0 440 212 564 628.5 0 564 213 3916 628.5 0 3916 214 3089 628.5 0 3089 215 5234 628.5 0 5234 216 602 628.5 0 602 217 402 628.5 0 402 218 223 628.5 0 223 219 214 628.5 0 214 220 192 628.5 0 192 221 301 628.5 0 301 222 257 628.5 0 257 223 362 628.5 0 362 224 450 628.5 0 450 225 861 628.5 0 861 226 1166 628.5 0 1166 227 1250 628.5 0 1250 228 433 628.5 0 433 229 376 628.5 0 376 230 266 628.5 0 266 231 271 628.5 0 271 232 187 628.5 0 187 233 173 628.5 0 173 234 155 628.5 0 155 235 191 628.5 0 191 236 168 628.5 0 168 237 234 628.5 0 234 238 164 628.5 0 164 239 159 628.5 0 159 240 140 628.5 0 140 241 176 628.5 0 176 242 165 628.5 0 165 243 193 628.5 0 193 244 145 628.5 0 145 245 112 628.5 0 112 246 168 628.5 0 168 247 186 628.5 0 186 248 230 628.5 0 230 249 259 628.5 0 259 250 452 628.5 0 452 251 413 628.5 0 413 252 710 628.5 0 710 253 479 628.5 0 479 254 598 628.5 0 598 255 453 628.5 0 453 256 358 628.5 0 358 257 181 628.5 0 181 258 173 628.5 0 173 259 176 628.5 0 176 260 219 628.5 0 219 261 177 628.5 0 177 262 259 628.5 0 259 263 366 628.5 0 366 264 455 628.5 0 455 265 517 628.5 0 517 266 486 628.5 0 486 267 269 628.5 0 269 268 296 628.5 0 296 269 309 628.5 0 309 270 368 628.5 0 368 271 384 628.5 0 384 272 295 628.5 0 295 273 276 628.5 0 276 274 246 628.5 0 246 275 232 628.5 0 232 276 362 628.5 0 362 277 336 628.5 0 336 278 378 628.5 0 378 279 300 628.5 0 300 280 231 628.5 0 231 281 291 628.5 0 291 282 330 628.5 0 330 283 315 628.5 0 315 284 324 628.5 0 324 285 279 628.5 0 279 286 331 628.5 0 331 287 266 628.5 0 266 288 226 628.5 0 226 289 353 628.5 0 353 290 379 628.5 0 379 291 424 628.5 0 424 292 312 628.5 0 312 293 257 628.5 0 257 294 284 628.5 0 284 295 336 628.5 0 336 296 285 628.5 0 285 297 257 628.5 0 257 298 207 628.5 0 207 299 230 628.5 0 230 300 258 628.5 0 258 301 564 628.5 0 564 Finished in 7.31 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 145,907 Reads with adapters: 63,440 (43.5%) Reads that were too short: 71 (0.0%) Reads written (passing filters): 63,369 (43.4%) Total basepairs processed: 39,098,980 bp Total written (filtered): 16,724,677 bp (42.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 63440 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 3 4 18 44 0.0 3 0 0 27 10 7 19 1186 0.0 3 0 996 110 57 23 20 55085 0.0 4 51072 3147 621 183 62 21 124 0.0 4 15 83 16 5 5 22 928 0.0 4 808 62 41 13 4 23 125 0.0 4 0 1 0 87 37 25 8 0.0 4 0 0 0 3 5 28 2 0.0 4 0 0 0 0 2 29 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 1 33 1 0.0 4 1 40 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 1 49 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 1 1 62 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 70 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 80 3 0.0 4 0 0 0 0 3 82 1 0.0 4 0 0 0 0 1 86 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 1 0 0 0 1 93 2 0.0 4 1 0 0 0 1 98 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 125 14 0.0 4 0 0 0 0 14 126 61 0.0 4 0 0 0 0 61 127 5706 0.0 4 0 0 0 0 5706 128 18 0.0 4 0 0 0 0 18 129 3 0.0 4 0 0 0 0 3 130 31 0.0 4 0 0 0 0 31 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 236 3 0.0 4 0 0 0 0 3 237 2 0.0 4 0 0 0 0 2 240 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 269 2 0.0 4 0 0 0 0 2 274 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 284 2 0.0 4 0 0 0 0 2 285 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.16 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 63,369 Reads with adapters: 4,123 (6.5%) Reads written (passing filters): 63,369 (100.0%) Total basepairs processed: 16,724,677 bp Total written (filtered): 16,544,392 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4123 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 21.4% C: 13.4% G: 63.0% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 456 990.1 0 456 4 65 247.5 0 65 5 3 61.9 0 3 9 27 0.2 0 3 24 10 62 0.1 1 4 58 11 1472 0.0 1 57 1415 12 1448 0.0 1 36 1412 13 24 0.0 1 0 24 14 2 0.0 1 0 2 15 2 0.0 1 0 2 16 1 0.0 1 0 1 20 1 0.0 1 0 1 152 15 0.0 1 12 3 169 1 0.0 1 0 1 171 1 0.0 1 1 210 10 0.0 1 8 2 230 1 0.0 1 1 248 1 0.0 1 0 1 259 1 0.0 1 1 261 513 0.0 1 476 37 262 16 0.0 1 14 2 263 1 0.0 1 1