This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.30 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 183,038 (7.1%) Reads that were too short: 39,218 (1.5%) Reads written (passing filters): 143,820 (5.6%) Total basepairs processed: 774,927,510 bp Total written (filtered): 39,685,781 bp (5.1%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 183038 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2016 628.5 0 2016 7 64471 628.5 0 64471 8 795 628.5 0 795 9 56705 628.5 0 56705 10 70 628.5 0 70 11 17 628.5 0 17 12 15 628.5 0 15 13 14 628.5 0 14 14 25 628.5 0 25 15 23 628.5 0 23 16 24 628.5 0 24 17 37 628.5 0 37 18 27 628.5 0 27 19 19 628.5 0 19 20 21 628.5 0 21 21 10 628.5 0 10 22 38 628.5 0 38 23 25 628.5 0 25 24 24 628.5 0 24 25 26 628.5 0 26 26 23 628.5 0 23 27 38 628.5 0 38 28 29 628.5 0 29 29 26 628.5 0 26 30 23 628.5 0 23 31 23 628.5 0 23 32 46 628.5 0 46 33 27 628.5 0 27 34 27 628.5 0 27 35 16 628.5 0 16 36 18 628.5 0 18 37 27 628.5 0 27 38 28 628.5 0 28 39 38 628.5 0 38 40 25 628.5 0 25 41 69 628.5 0 69 42 183 628.5 0 183 43 117 628.5 0 117 44 36 628.5 0 36 45 89 628.5 0 89 46 32 628.5 0 32 47 21 628.5 0 21 48 41 628.5 0 41 49 32 628.5 0 32 50 29 628.5 0 29 51 37 628.5 0 37 52 20 628.5 0 20 53 29 628.5 0 29 54 12 628.5 0 12 55 19 628.5 0 19 56 13 628.5 0 13 57 43 628.5 0 43 58 41 628.5 0 41 59 26 628.5 0 26 60 31 628.5 0 31 61 31 628.5 0 31 62 31 628.5 0 31 63 29 628.5 0 29 64 24 628.5 0 24 65 23 628.5 0 23 66 25 628.5 0 25 67 100 628.5 0 100 68 27 628.5 0 27 69 50 628.5 0 50 70 50 628.5 0 50 71 25 628.5 0 25 72 41 628.5 0 41 73 36 628.5 0 36 74 46 628.5 0 46 75 48 628.5 0 48 76 53 628.5 0 53 77 89 628.5 0 89 78 117 628.5 0 117 79 93 628.5 0 93 80 96 628.5 0 96 81 87 628.5 0 87 82 128 628.5 0 128 83 98 628.5 0 98 84 89 628.5 0 89 85 71 628.5 0 71 86 61 628.5 0 61 87 61 628.5 0 61 88 50 628.5 0 50 89 46 628.5 0 46 90 53 628.5 0 53 91 56 628.5 0 56 92 46 628.5 0 46 93 49 628.5 0 49 94 42 628.5 0 42 95 34 628.5 0 34 96 53 628.5 0 53 97 51 628.5 0 51 98 66 628.5 0 66 99 60 628.5 0 60 100 63 628.5 0 63 101 70 628.5 0 70 102 72 628.5 0 72 103 78 628.5 0 78 104 100 628.5 0 100 105 110 628.5 0 110 106 126 628.5 0 126 107 113 628.5 0 113 108 101 628.5 0 101 109 94 628.5 0 94 110 106 628.5 0 106 111 99 628.5 0 99 112 87 628.5 0 87 113 105 628.5 0 105 114 86 628.5 0 86 115 84 628.5 0 84 116 152 628.5 0 152 117 83 628.5 0 83 118 84 628.5 0 84 119 126 628.5 0 126 120 108 628.5 0 108 121 89 628.5 0 89 122 74 628.5 0 74 123 116 628.5 0 116 124 111 628.5 0 111 125 163 628.5 0 163 126 140 628.5 0 140 127 106 628.5 0 106 128 186 628.5 0 186 129 93 628.5 0 93 130 108 628.5 0 108 131 146 628.5 0 146 132 155 628.5 0 155 133 1198 628.5 0 1198 134 742 628.5 0 742 135 1609 628.5 0 1609 136 122 628.5 0 122 137 83 628.5 0 83 138 101 628.5 0 101 139 91 628.5 0 91 140 89 628.5 0 89 141 95 628.5 0 95 142 84 628.5 0 84 143 93 628.5 0 93 144 116 628.5 0 116 145 113 628.5 0 113 146 101 628.5 0 101 147 140 628.5 0 140 148 111 628.5 0 111 149 93 628.5 0 93 150 86 628.5 0 86 151 81 628.5 0 81 152 109 628.5 0 109 153 86 628.5 0 86 154 116 628.5 0 116 155 88 628.5 0 88 156 117 628.5 0 117 157 128 628.5 0 128 158 111 628.5 0 111 159 208 628.5 0 208 160 207 628.5 0 207 161 171 628.5 0 171 162 362 628.5 0 362 163 295 628.5 0 295 164 449 628.5 0 449 165 116 628.5 0 116 166 112 628.5 0 112 167 128 628.5 0 128 168 84 628.5 0 84 169 115 628.5 0 115 170 115 628.5 0 115 171 135 628.5 0 135 172 149 628.5 0 149 173 140 628.5 0 140 174 95 628.5 0 95 175 103 628.5 0 103 176 75 628.5 0 75 177 88 628.5 0 88 178 105 628.5 0 105 179 136 628.5 0 136 180 154 628.5 0 154 181 97 628.5 0 97 182 120 628.5 0 120 183 154 628.5 0 154 184 181 628.5 0 181 185 184 628.5 0 184 186 173 628.5 0 173 187 194 628.5 0 194 188 88 628.5 0 88 189 171 628.5 0 171 190 138 628.5 0 138 191 128 628.5 0 128 192 121 628.5 0 121 193 184 628.5 0 184 194 90 628.5 0 90 195 105 628.5 0 105 196 133 628.5 0 133 197 91 628.5 0 91 198 112 628.5 0 112 199 112 628.5 0 112 200 132 628.5 0 132 201 162 628.5 0 162 202 119 628.5 0 119 203 140 628.5 0 140 204 116 628.5 0 116 205 95 628.5 0 95 206 134 628.5 0 134 207 174 628.5 0 174 208 200 628.5 0 200 209 227 628.5 0 227 210 229 628.5 0 229 211 608 628.5 0 608 212 626 628.5 0 626 213 7203 628.5 0 7203 214 4545 628.5 0 4545 215 8715 628.5 0 8715 216 434 628.5 0 434 217 205 628.5 0 205 218 197 628.5 0 197 219 142 628.5 0 142 220 124 628.5 0 124 221 163 628.5 0 163 222 142 628.5 0 142 223 166 628.5 0 166 224 190 628.5 0 190 225 424 628.5 0 424 226 674 628.5 0 674 227 709 628.5 0 709 228 256 628.5 0 256 229 211 628.5 0 211 230 178 628.5 0 178 231 176 628.5 0 176 232 117 628.5 0 117 233 103 628.5 0 103 234 122 628.5 0 122 235 124 628.5 0 124 236 150 628.5 0 150 237 139 628.5 0 139 238 119 628.5 0 119 239 128 628.5 0 128 240 115 628.5 0 115 241 150 628.5 0 150 242 127 628.5 0 127 243 146 628.5 0 146 244 115 628.5 0 115 245 102 628.5 0 102 246 132 628.5 0 132 247 133 628.5 0 133 248 154 628.5 0 154 249 169 628.5 0 169 250 327 628.5 0 327 251 231 628.5 0 231 252 359 628.5 0 359 253 190 628.5 0 190 254 180 628.5 0 180 255 172 628.5 0 172 256 155 628.5 0 155 257 149 628.5 0 149 258 128 628.5 0 128 259 140 628.5 0 140 260 145 628.5 0 145 261 150 628.5 0 150 262 152 628.5 0 152 263 180 628.5 0 180 264 181 628.5 0 181 265 209 628.5 0 209 266 207 628.5 0 207 267 155 628.5 0 155 268 168 628.5 0 168 269 156 628.5 0 156 270 153 628.5 0 153 271 182 628.5 0 182 272 143 628.5 0 143 273 147 628.5 0 147 274 154 628.5 0 154 275 171 628.5 0 171 276 231 628.5 0 231 277 194 628.5 0 194 278 213 628.5 0 213 279 154 628.5 0 154 280 160 628.5 0 160 281 162 628.5 0 162 282 184 628.5 0 184 283 200 628.5 0 200 284 168 628.5 0 168 285 138 628.5 0 138 286 165 628.5 0 165 287 150 628.5 0 150 288 154 628.5 0 154 289 212 628.5 0 212 290 255 628.5 0 255 291 221 628.5 0 221 292 157 628.5 0 157 293 129 628.5 0 129 294 126 628.5 0 126 295 201 628.5 0 201 296 152 628.5 0 152 297 157 628.5 0 157 298 161 628.5 0 161 299 141 628.5 0 141 300 179 628.5 0 179 301 303 628.5 0 303 Finished in 6.53 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 143,820 Reads with adapters: 58,264 (40.5%) Reads that were too short: 170 (0.1%) Reads written (passing filters): 58,094 (40.4%) Total basepairs processed: 39,685,781 bp Total written (filtered): 15,974,046 bp (40.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58264 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 10 0.0 3 0 0 10 16 302 0.0 3 0 292 7 3 17 57545 0.0 3 56426 1080 35 4 18 137 0.0 3 14 120 3 19 30 0.0 3 3 0 27 20 38 0.0 3 0 0 0 38 21 2 0.0 3 1 0 0 1 22 1 0.0 3 1 24 2 0.0 3 0 0 1 1 28 1 0.0 3 1 32 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 1 0.0 3 1 48 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 2 0.0 3 0 1 0 1 59 1 0.0 3 1 65 1 0.0 3 0 0 0 1 67 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 84 1 0.0 3 1 86 2 0.0 3 1 0 0 1 89 1 0.0 3 1 98 1 0.0 3 0 1 99 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 4 0.0 3 0 0 1 3 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 154 3 0.0 3 0 0 0 3 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 131 0.0 3 0 0 2 129 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 1 221 1 0.0 3 0 0 0 1 248 4 0.0 3 0 0 0 4 266 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.06 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 58,094 Reads with adapters: 608 (1.0%) Reads written (passing filters): 58,094 (100.0%) Total basepairs processed: 15,974,046 bp Total written (filtered): 15,972,133 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 608 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.1% C: 8.4% G: 25.2% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 529 907.7 0 529 4 77 226.9 0 77 6 1 14.2 0 1 12 1 0.0 1 0 1