This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.42 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 384,596 (14.9%) Reads that were too short: 319,488 (12.4%) Reads written (passing filters): 65,108 (2.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 14,742,186 bp (1.9%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 384596 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 872 628.5 0 872 7 5721 628.5 0 5721 8 15775 628.5 0 15775 9 12091 628.5 0 12091 10 65 628.5 0 65 11 56 628.5 0 56 12 52 628.5 0 52 13 62 628.5 0 62 14 55 628.5 0 55 15 31 628.5 0 31 16 61 628.5 0 61 17 42 628.5 0 42 18 67 628.5 0 67 19 58 628.5 0 58 20 129 628.5 0 129 21 62 628.5 0 62 22 114 628.5 0 114 23 53 628.5 0 53 24 46 628.5 0 46 25 42 628.5 0 42 26 56 628.5 0 56 27 72 628.5 0 72 28 47 628.5 0 47 29 35 628.5 0 35 30 34 628.5 0 34 31 48 628.5 0 48 32 44 628.5 0 44 33 196 628.5 0 196 34 50 628.5 0 50 35 63 628.5 0 63 36 34 628.5 0 34 37 25 628.5 0 25 38 51 628.5 0 51 39 42 628.5 0 42 40 35 628.5 0 35 41 39 628.5 0 39 42 51 628.5 0 51 43 39 628.5 0 39 44 35 628.5 0 35 45 42 628.5 0 42 46 50 628.5 0 50 47 52 628.5 0 52 48 52 628.5 0 52 49 58 628.5 0 58 50 50 628.5 0 50 51 50 628.5 0 50 52 35 628.5 0 35 53 41 628.5 0 41 54 41 628.5 0 41 55 40 628.5 0 40 56 19 628.5 0 19 57 40 628.5 0 40 58 28 628.5 0 28 59 34 628.5 0 34 60 37 628.5 0 37 61 37 628.5 0 37 62 43 628.5 0 43 63 82 628.5 0 82 64 93 628.5 0 93 65 44 628.5 0 44 66 75 628.5 0 75 67 90 628.5 0 90 68 51 628.5 0 51 69 49 628.5 0 49 70 29 628.5 0 29 71 51 628.5 0 51 72 74 628.5 0 74 73 62 628.5 0 62 74 76 628.5 0 76 75 86 628.5 0 86 76 82 628.5 0 82 77 69 628.5 0 69 78 61 628.5 0 61 79 51 628.5 0 51 80 46 628.5 0 46 81 33 628.5 0 33 82 56 628.5 0 56 83 37 628.5 0 37 84 33 628.5 0 33 85 60 628.5 0 60 86 60 628.5 0 60 87 55 628.5 0 55 88 75 628.5 0 75 89 68 628.5 0 68 90 59 628.5 0 59 91 84 628.5 0 84 92 72 628.5 0 72 93 82 628.5 0 82 94 127 628.5 0 127 95 186 628.5 0 186 96 95 628.5 0 95 97 66 628.5 0 66 98 63 628.5 0 63 99 102 628.5 0 102 100 48 628.5 0 48 101 52 628.5 0 52 102 48 628.5 0 48 103 44 628.5 0 44 104 27 628.5 0 27 105 48 628.5 0 48 106 48 628.5 0 48 107 69 628.5 0 69 108 48 628.5 0 48 109 46 628.5 0 46 110 41 628.5 0 41 111 42 628.5 0 42 112 33 628.5 0 33 113 50 628.5 0 50 114 25 628.5 0 25 115 25 628.5 0 25 116 39 628.5 0 39 117 40 628.5 0 40 118 57 628.5 0 57 119 59 628.5 0 59 120 78 628.5 0 78 121 67 628.5 0 67 122 65 628.5 0 65 123 60 628.5 0 60 124 92 628.5 0 92 125 119 628.5 0 119 126 239 628.5 0 239 127 251 628.5 0 251 128 106 628.5 0 106 129 121 628.5 0 121 130 167 628.5 0 167 131 4092 628.5 0 4092 132 519 628.5 0 519 133 602 628.5 0 602 134 67 628.5 0 67 135 72 628.5 0 72 136 66 628.5 0 66 137 58 628.5 0 58 138 48 628.5 0 48 139 54 628.5 0 54 140 55 628.5 0 55 141 74 628.5 0 74 142 52 628.5 0 52 143 78 628.5 0 78 144 63 628.5 0 63 145 86 628.5 0 86 146 90 628.5 0 90 147 131 628.5 0 131 148 68 628.5 0 68 149 56 628.5 0 56 150 32 628.5 0 32 151 37 628.5 0 37 152 49 628.5 0 49 153 75 628.5 0 75 154 111 628.5 0 111 155 170 628.5 0 170 156 207 628.5 0 207 157 55 628.5 0 55 158 46 628.5 0 46 159 40 628.5 0 40 160 55 628.5 0 55 161 56 628.5 0 56 162 54 628.5 0 54 163 37 628.5 0 37 164 46 628.5 0 46 165 63 628.5 0 63 166 66 628.5 0 66 167 57 628.5 0 57 168 65 628.5 0 65 169 76 628.5 0 76 170 59 628.5 0 59 171 56 628.5 0 56 172 55 628.5 0 55 173 131 628.5 0 131 174 216 628.5 0 216 175 269 628.5 0 269 176 167 628.5 0 167 177 82 628.5 0 82 178 76 628.5 0 76 179 339 628.5 0 339 180 470 628.5 0 470 181 510 628.5 0 510 182 153 628.5 0 153 183 111 628.5 0 111 184 84 628.5 0 84 185 290 628.5 0 290 186 182 628.5 0 182 187 406 628.5 0 406 188 125 628.5 0 125 189 136 628.5 0 136 190 154 628.5 0 154 191 238 628.5 0 238 192 497 628.5 0 497 193 3142 628.5 0 3142 194 1703 628.5 0 1703 195 3108 628.5 0 3108 196 231 628.5 0 231 197 137 628.5 0 137 198 131 628.5 0 131 199 483 628.5 0 483 200 700 628.5 0 700 201 902 628.5 0 902 202 717 628.5 0 717 203 120 628.5 0 120 204 80 628.5 0 80 205 172 628.5 0 172 206 191 628.5 0 191 207 209 628.5 0 209 208 144 628.5 0 144 209 76 628.5 0 76 210 102 628.5 0 102 211 146 628.5 0 146 212 154 628.5 0 154 213 289 628.5 0 289 214 167 628.5 0 167 215 128 628.5 0 128 216 107 628.5 0 107 217 133 628.5 0 133 218 153 628.5 0 153 219 232 628.5 0 232 220 285 628.5 0 285 221 382 628.5 0 382 222 450 628.5 0 450 223 519 628.5 0 519 224 824 628.5 0 824 225 281 628.5 0 281 226 338 628.5 0 338 227 558 628.5 0 558 228 323 628.5 0 323 229 390 628.5 0 390 230 683 628.5 0 683 231 862 628.5 0 862 232 4424 628.5 0 4424 233 5193 628.5 0 5193 234 3787 628.5 0 3787 235 1417 628.5 0 1417 236 2091 628.5 0 2091 237 25805 628.5 0 25805 238 33713 628.5 0 33713 239 47347 628.5 0 47347 240 1456 628.5 0 1456 241 767 628.5 0 767 242 551 628.5 0 551 243 787 628.5 0 787 244 684 628.5 0 684 245 494 628.5 0 494 246 396 628.5 0 396 247 517 628.5 0 517 248 456 628.5 0 456 249 755 628.5 0 755 250 1213 628.5 0 1213 251 1416 628.5 0 1416 252 1825 628.5 0 1825 253 1985 628.5 0 1985 254 1915 628.5 0 1915 255 1632 628.5 0 1632 256 2290 628.5 0 2290 257 41925 628.5 0 41925 258 60171 628.5 0 60171 259 43857 628.5 0 43857 260 14833 628.5 0 14833 261 209 628.5 0 209 262 133 628.5 0 133 263 135 628.5 0 135 264 56 628.5 0 56 265 41 628.5 0 41 266 73 628.5 0 73 267 100 628.5 0 100 268 193 628.5 0 193 269 164 628.5 0 164 270 155 628.5 0 155 271 102 628.5 0 102 272 99 628.5 0 99 273 117 628.5 0 117 274 72 628.5 0 72 275 85 628.5 0 85 276 75 628.5 0 75 277 51 628.5 0 51 278 76 628.5 0 76 279 82 628.5 0 82 280 90 628.5 0 90 281 99 628.5 0 99 282 112 628.5 0 112 283 122 628.5 0 122 284 95 628.5 0 95 285 161 628.5 0 161 286 195 628.5 0 195 287 341 628.5 0 341 288 168 628.5 0 168 289 267 628.5 0 267 290 407 628.5 0 407 291 606 628.5 0 606 292 346 628.5 0 346 293 452 628.5 0 452 294 201 628.5 0 201 295 128 628.5 0 128 296 140 628.5 0 140 297 109 628.5 0 109 298 72 628.5 0 72 299 77 628.5 0 77 300 173 628.5 0 173 301 192 628.5 0 192 Finished in 3.10 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 65,108 Reads with adapters: 13,514 (20.8%) Reads that were too short: 42 (0.1%) Reads written (passing filters): 13,472 (20.7%) Total basepairs processed: 14,742,186 bp Total written (filtered): 3,635,775 bp (24.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13514 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 5 18 19 0.0 3 0 0 9 7 3 19 327 0.0 3 0 263 32 23 9 20 12678 0.0 4 11379 980 229 67 23 21 47 0.0 4 10 26 5 3 3 22 8 0.0 4 5 0 1 0 2 23 108 0.0 4 2 0 1 59 46 40 2 0.0 4 0 0 0 0 2 41 2 0.0 4 0 0 0 0 2 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 1 110 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 181 0.0 4 0 0 0 0 181 129 83 0.0 4 0 0 0 0 83 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 1 158 4 0.0 4 0 0 0 0 4 161 2 0.0 4 0 0 0 0 2 175 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 1 3 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 245 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 1 257 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 13,472 Reads with adapters: 3,068 (22.8%) Reads written (passing filters): 13,472 (100.0%) Total basepairs processed: 3,635,775 bp Total written (filtered): 3,602,561 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3068 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.6% C: 10.6% G: 83.7% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 210.5 0 14 4 6 52.6 0 6 6 12 3.3 0 12 7 1 0.8 0 1 9 509 0.1 0 58 451 10 2085 0.0 1 170 1915 11 401 0.0 1 17 384 12 10 0.0 1 0 10 13 9 0.0 1 0 9 14 3 0.0 1 0 3 15 1 0.0 1 0 1 22 1 0.0 1 0 1 95 1 0.0 1 0 1 150 4 0.0 1 4 151 1 0.0 1 0 1 171 1 0.0 1 0 1 177 1 0.0 1 1 208 5 0.0 1 5 209 2 0.0 1 2 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.