This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.80 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 458,694 (17.8%) Reads that were too short: 388,854 (15.1%) Reads written (passing filters): 69,840 (2.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 15,955,053 bp (2.1%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 458694 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 896 628.5 0 896 7 5330 628.5 0 5330 8 15475 628.5 0 15475 9 14794 628.5 0 14794 10 73 628.5 0 73 11 82 628.5 0 82 12 61 628.5 0 61 13 68 628.5 0 68 14 73 628.5 0 73 15 48 628.5 0 48 16 71 628.5 0 71 17 93 628.5 0 93 18 69 628.5 0 69 19 79 628.5 0 79 20 92 628.5 0 92 21 67 628.5 0 67 22 72 628.5 0 72 23 60 628.5 0 60 24 77 628.5 0 77 25 59 628.5 0 59 26 66 628.5 0 66 27 64 628.5 0 64 28 51 628.5 0 51 29 71 628.5 0 71 30 50 628.5 0 50 31 53 628.5 0 53 32 38 628.5 0 38 33 57 628.5 0 57 34 58 628.5 0 58 35 91 628.5 0 91 36 76 628.5 0 76 37 62 628.5 0 62 38 68 628.5 0 68 39 64 628.5 0 64 40 48 628.5 0 48 41 58 628.5 0 58 42 60 628.5 0 60 43 54 628.5 0 54 44 45 628.5 0 45 45 46 628.5 0 46 46 62 628.5 0 62 47 78 628.5 0 78 48 91 628.5 0 91 49 50 628.5 0 50 50 55 628.5 0 55 51 80 628.5 0 80 52 55 628.5 0 55 53 66 628.5 0 66 54 62 628.5 0 62 55 56 628.5 0 56 56 45 628.5 0 45 57 78 628.5 0 78 58 60 628.5 0 60 59 51 628.5 0 51 60 50 628.5 0 50 61 65 628.5 0 65 62 55 628.5 0 55 63 128 628.5 0 128 64 117 628.5 0 117 65 52 628.5 0 52 66 97 628.5 0 97 67 116 628.5 0 116 68 68 628.5 0 68 69 64 628.5 0 64 70 58 628.5 0 58 71 93 628.5 0 93 72 84 628.5 0 84 73 72 628.5 0 72 74 72 628.5 0 72 75 71 628.5 0 71 76 79 628.5 0 79 77 98 628.5 0 98 78 65 628.5 0 65 79 75 628.5 0 75 80 53 628.5 0 53 81 61 628.5 0 61 82 61 628.5 0 61 83 58 628.5 0 58 84 55 628.5 0 55 85 90 628.5 0 90 86 65 628.5 0 65 87 91 628.5 0 91 88 81 628.5 0 81 89 90 628.5 0 90 90 92 628.5 0 92 91 82 628.5 0 82 92 84 628.5 0 84 93 82 628.5 0 82 94 73 628.5 0 73 95 73 628.5 0 73 96 58 628.5 0 58 97 84 628.5 0 84 98 69 628.5 0 69 99 90 628.5 0 90 100 82 628.5 0 82 101 71 628.5 0 71 102 74 628.5 0 74 103 63 628.5 0 63 104 45 628.5 0 45 105 67 628.5 0 67 106 85 628.5 0 85 107 75 628.5 0 75 108 71 628.5 0 71 109 66 628.5 0 66 110 74 628.5 0 74 111 61 628.5 0 61 112 70 628.5 0 70 113 75 628.5 0 75 114 76 628.5 0 76 115 69 628.5 0 69 116 80 628.5 0 80 117 84 628.5 0 84 118 78 628.5 0 78 119 69 628.5 0 69 120 92 628.5 0 92 121 82 628.5 0 82 122 83 628.5 0 83 123 94 628.5 0 94 124 105 628.5 0 105 125 124 628.5 0 124 126 158 628.5 0 158 127 146 628.5 0 146 128 87 628.5 0 87 129 145 628.5 0 145 130 181 628.5 0 181 131 5390 628.5 0 5390 132 518 628.5 0 518 133 418 628.5 0 418 134 102 628.5 0 102 135 113 628.5 0 113 136 88 628.5 0 88 137 112 628.5 0 112 138 79 628.5 0 79 139 95 628.5 0 95 140 91 628.5 0 91 141 127 628.5 0 127 142 108 628.5 0 108 143 116 628.5 0 116 144 106 628.5 0 106 145 93 628.5 0 93 146 119 628.5 0 119 147 141 628.5 0 141 148 115 628.5 0 115 149 105 628.5 0 105 150 105 628.5 0 105 151 129 628.5 0 129 152 115 628.5 0 115 153 134 628.5 0 134 154 149 628.5 0 149 155 142 628.5 0 142 156 160 628.5 0 160 157 97 628.5 0 97 158 113 628.5 0 113 159 114 628.5 0 114 160 126 628.5 0 126 161 104 628.5 0 104 162 123 628.5 0 123 163 124 628.5 0 124 164 125 628.5 0 125 165 152 628.5 0 152 166 127 628.5 0 127 167 152 628.5 0 152 168 141 628.5 0 141 169 149 628.5 0 149 170 110 628.5 0 110 171 139 628.5 0 139 172 148 628.5 0 148 173 199 628.5 0 199 174 228 628.5 0 228 175 253 628.5 0 253 176 205 628.5 0 205 177 144 628.5 0 144 178 133 628.5 0 133 179 172 628.5 0 172 180 290 628.5 0 290 181 309 628.5 0 309 182 163 628.5 0 163 183 159 628.5 0 159 184 158 628.5 0 158 185 381 628.5 0 381 186 272 628.5 0 272 187 443 628.5 0 443 188 211 628.5 0 211 189 194 628.5 0 194 190 242 628.5 0 242 191 273 628.5 0 273 192 351 628.5 0 351 193 2084 628.5 0 2084 194 1552 628.5 0 1552 195 3042 628.5 0 3042 196 230 628.5 0 230 197 222 628.5 0 222 198 191 628.5 0 191 199 315 628.5 0 315 200 449 628.5 0 449 201 450 628.5 0 450 202 356 628.5 0 356 203 155 628.5 0 155 204 135 628.5 0 135 205 184 628.5 0 184 206 205 628.5 0 205 207 226 628.5 0 226 208 178 628.5 0 178 209 154 628.5 0 154 210 192 628.5 0 192 211 225 628.5 0 225 212 209 628.5 0 209 213 300 628.5 0 300 214 234 628.5 0 234 215 242 628.5 0 242 216 199 628.5 0 199 217 245 628.5 0 245 218 242 628.5 0 242 219 337 628.5 0 337 220 325 628.5 0 325 221 354 628.5 0 354 222 463 628.5 0 463 223 594 628.5 0 594 224 923 628.5 0 923 225 411 628.5 0 411 226 450 628.5 0 450 227 803 628.5 0 803 228 466 628.5 0 466 229 474 628.5 0 474 230 713 628.5 0 713 231 1039 628.5 0 1039 232 5689 628.5 0 5689 233 7137 628.5 0 7137 234 4854 628.5 0 4854 235 1445 628.5 0 1445 236 2038 628.5 0 2038 237 30070 628.5 0 30070 238 43369 628.5 0 43369 239 52756 628.5 0 52756 240 1363 628.5 0 1363 241 761 628.5 0 761 242 747 628.5 0 747 243 987 628.5 0 987 244 942 628.5 0 942 245 819 628.5 0 819 246 545 628.5 0 545 247 683 628.5 0 683 248 740 628.5 0 740 249 1103 628.5 0 1103 250 1739 628.5 0 1739 251 2023 628.5 0 2023 252 2262 628.5 0 2262 253 2538 628.5 0 2538 254 2274 628.5 0 2274 255 1775 628.5 0 1775 256 2314 628.5 0 2314 257 44845 628.5 0 44845 258 72462 628.5 0 72462 259 55750 628.5 0 55750 260 25065 628.5 0 25065 261 234 628.5 0 234 262 151 628.5 0 151 263 168 628.5 0 168 264 147 628.5 0 147 265 145 628.5 0 145 266 152 628.5 0 152 267 168 628.5 0 168 268 246 628.5 0 246 269 301 628.5 0 301 270 230 628.5 0 230 271 152 628.5 0 152 272 158 628.5 0 158 273 160 628.5 0 160 274 139 628.5 0 139 275 133 628.5 0 133 276 181 628.5 0 181 277 154 628.5 0 154 278 136 628.5 0 136 279 179 628.5 0 179 280 203 628.5 0 203 281 176 628.5 0 176 282 166 628.5 0 166 283 166 628.5 0 166 284 179 628.5 0 179 285 157 628.5 0 157 286 162 628.5 0 162 287 170 628.5 0 170 288 164 628.5 0 164 289 204 628.5 0 204 290 311 628.5 0 311 291 732 628.5 0 732 292 466 628.5 0 466 293 605 628.5 0 605 294 297 628.5 0 297 295 184 628.5 0 184 296 172 628.5 0 172 297 172 628.5 0 172 298 160 628.5 0 160 299 171 628.5 0 171 300 199 628.5 0 199 301 176 628.5 0 176 Finished in 2.96 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 69,840 Reads with adapters: 20,359 (29.2%) Reads that were too short: 37 (0.1%) Reads written (passing filters): 20,322 (29.1%) Total basepairs processed: 15,955,053 bp Total written (filtered): 5,600,780 bp (35.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20359 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 104 0.0 3 0 102 2 17 14850 0.0 3 14541 296 11 2 18 72 0.0 3 1 66 1 4 19 5247 0.0 3 5142 86 18 1 20 19 0.0 3 1 7 0 11 21 2 0.0 3 0 0 2 22 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 60 11 0.0 3 0 0 0 11 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 100 2 0.0 3 0 0 0 2 102 1 0.0 3 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 248 2 0.0 3 0 1 1 249 1 0.0 3 0 1 251 2 0.0 3 0 0 1 1 282 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.71 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 20,322 Reads with adapters: 173 (0.9%) Reads written (passing filters): 20,322 (100.0%) Total basepairs processed: 5,600,780 bp Total written (filtered): 5,600,232 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 173 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.7% C: 5.8% G: 16.8% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 149 317.5 0 149 4 20 79.4 0 20 5 3 19.8 0 3 6 1 5.0 0 1