This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.13 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 88,921 (3.5%) Reads that were too short: 17,899 (0.7%) Reads written (passing filters): 71,022 (2.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 17,854,223 bp (2.3%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 88921 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 790 628.5 0 790 7 20560 628.5 0 20560 8 723 628.5 0 723 9 23913 628.5 0 23913 10 33 628.5 0 33 11 17 628.5 0 17 12 15 628.5 0 15 13 34 628.5 0 34 14 15 628.5 0 15 15 17 628.5 0 17 16 31 628.5 0 31 17 34 628.5 0 34 18 75 628.5 0 75 19 21 628.5 0 21 20 13 628.5 0 13 21 25 628.5 0 25 22 31 628.5 0 31 23 29 628.5 0 29 24 26 628.5 0 26 25 23 628.5 0 23 26 325 628.5 0 325 27 173 628.5 0 173 28 655 628.5 0 655 29 178 628.5 0 178 30 229 628.5 0 229 31 373 628.5 0 373 32 13 628.5 0 13 33 19 628.5 0 19 34 19 628.5 0 19 35 26 628.5 0 26 36 19 628.5 0 19 37 20 628.5 0 20 38 22 628.5 0 22 39 8 628.5 0 8 40 16 628.5 0 16 41 9 628.5 0 9 42 22 628.5 0 22 43 14 628.5 0 14 44 26 628.5 0 26 45 31 628.5 0 31 46 11 628.5 0 11 47 14 628.5 0 14 48 18 628.5 0 18 49 19 628.5 0 19 50 15 628.5 0 15 51 27 628.5 0 27 52 24 628.5 0 24 53 28 628.5 0 28 54 15 628.5 0 15 55 19 628.5 0 19 56 20 628.5 0 20 57 22 628.5 0 22 58 24 628.5 0 24 59 15 628.5 0 15 60 26 628.5 0 26 61 21 628.5 0 21 62 31 628.5 0 31 63 23 628.5 0 23 64 26 628.5 0 26 65 40 628.5 0 40 66 20 628.5 0 20 67 32 628.5 0 32 68 41 628.5 0 41 69 266 628.5 0 266 70 119 628.5 0 119 71 305 628.5 0 305 72 39 628.5 0 39 73 19 628.5 0 19 74 22 628.5 0 22 75 36 628.5 0 36 76 30 628.5 0 30 77 86 628.5 0 86 78 64 628.5 0 64 79 74 628.5 0 74 80 22 628.5 0 22 81 31 628.5 0 31 82 34 628.5 0 34 83 32 628.5 0 32 84 24 628.5 0 24 85 51 628.5 0 51 86 42 628.5 0 42 87 45 628.5 0 45 88 33 628.5 0 33 89 33 628.5 0 33 90 35 628.5 0 35 91 38 628.5 0 38 92 33 628.5 0 33 93 26 628.5 0 26 94 27 628.5 0 27 95 31 628.5 0 31 96 42 628.5 0 42 97 71 628.5 0 71 98 47 628.5 0 47 99 62 628.5 0 62 100 36 628.5 0 36 101 48 628.5 0 48 102 31 628.5 0 31 103 20 628.5 0 20 104 26 628.5 0 26 105 28 628.5 0 28 106 19 628.5 0 19 107 30 628.5 0 30 108 24 628.5 0 24 109 27 628.5 0 27 110 30 628.5 0 30 111 53 628.5 0 53 112 63 628.5 0 63 113 46 628.5 0 46 114 311 628.5 0 311 115 405 628.5 0 405 116 195 628.5 0 195 117 32 628.5 0 32 118 37 628.5 0 37 119 25 628.5 0 25 120 25 628.5 0 25 121 23 628.5 0 23 122 33 628.5 0 33 123 52 628.5 0 52 124 54 628.5 0 54 125 67 628.5 0 67 126 81 628.5 0 81 127 103 628.5 0 103 128 198 628.5 0 198 129 122 628.5 0 122 130 89 628.5 0 89 131 85 628.5 0 85 132 89 628.5 0 89 133 246 628.5 0 246 134 116 628.5 0 116 135 90 628.5 0 90 136 104 628.5 0 104 137 151 628.5 0 151 138 689 628.5 0 689 139 1743 628.5 0 1743 140 3278 628.5 0 3278 141 2371 628.5 0 2371 142 346 628.5 0 346 143 378 628.5 0 378 144 516 628.5 0 516 145 474 628.5 0 474 146 74 628.5 0 74 147 182 628.5 0 182 148 385 628.5 0 385 149 1190 628.5 0 1190 150 1852 628.5 0 1852 151 654 628.5 0 654 152 113 628.5 0 113 153 86 628.5 0 86 154 42 628.5 0 42 155 62 628.5 0 62 156 31 628.5 0 31 157 40 628.5 0 40 158 35 628.5 0 35 159 38 628.5 0 38 160 32 628.5 0 32 161 21 628.5 0 21 162 22 628.5 0 22 163 25 628.5 0 25 164 21 628.5 0 21 165 26 628.5 0 26 166 29 628.5 0 29 167 16 628.5 0 16 168 30 628.5 0 30 169 34 628.5 0 34 170 35 628.5 0 35 171 19 628.5 0 19 172 24 628.5 0 24 173 39 628.5 0 39 174 24 628.5 0 24 175 35 628.5 0 35 176 62 628.5 0 62 177 33 628.5 0 33 178 41 628.5 0 41 179 36 628.5 0 36 180 41 628.5 0 41 181 29 628.5 0 29 182 31 628.5 0 31 183 42 628.5 0 42 184 87 628.5 0 87 185 78 628.5 0 78 186 87 628.5 0 87 187 41 628.5 0 41 188 52 628.5 0 52 189 129 628.5 0 129 190 203 628.5 0 203 191 363 628.5 0 363 192 70 628.5 0 70 193 44 628.5 0 44 194 42 628.5 0 42 195 48 628.5 0 48 196 52 628.5 0 52 197 44 628.5 0 44 198 53 628.5 0 53 199 60 628.5 0 60 200 27 628.5 0 27 201 30 628.5 0 30 202 49 628.5 0 49 203 60 628.5 0 60 204 85 628.5 0 85 205 67 628.5 0 67 206 51 628.5 0 51 207 65 628.5 0 65 208 55 628.5 0 55 209 213 628.5 0 213 210 281 628.5 0 281 211 185 628.5 0 185 212 95 628.5 0 95 213 135 628.5 0 135 214 202 628.5 0 202 215 317 628.5 0 317 216 34 628.5 0 34 217 52 628.5 0 52 218 41 628.5 0 41 219 61 628.5 0 61 220 54 628.5 0 54 221 43 628.5 0 43 222 69 628.5 0 69 223 71 628.5 0 71 224 78 628.5 0 78 225 52 628.5 0 52 226 48 628.5 0 48 227 39 628.5 0 39 228 71 628.5 0 71 229 69 628.5 0 69 230 107 628.5 0 107 231 60 628.5 0 60 232 67 628.5 0 67 233 226 628.5 0 226 234 487 628.5 0 487 235 526 628.5 0 526 236 280 628.5 0 280 237 69 628.5 0 69 238 72 628.5 0 72 239 72 628.5 0 72 240 96 628.5 0 96 241 98 628.5 0 98 242 110 628.5 0 110 243 120 628.5 0 120 244 102 628.5 0 102 245 126 628.5 0 126 246 101 628.5 0 101 247 77 628.5 0 77 248 135 628.5 0 135 249 134 628.5 0 134 250 239 628.5 0 239 251 102 628.5 0 102 252 121 628.5 0 121 253 887 628.5 0 887 254 1268 628.5 0 1268 255 2141 628.5 0 2141 256 369 628.5 0 369 257 88 628.5 0 88 258 81 628.5 0 81 259 81 628.5 0 81 260 93 628.5 0 93 261 81 628.5 0 81 262 105 628.5 0 105 263 69 628.5 0 69 264 96 628.5 0 96 265 75 628.5 0 75 266 97 628.5 0 97 267 101 628.5 0 101 268 154 628.5 0 154 269 143 628.5 0 143 270 150 628.5 0 150 271 125 628.5 0 125 272 151 628.5 0 151 273 482 628.5 0 482 274 666 628.5 0 666 275 439 628.5 0 439 276 223 628.5 0 223 277 41 628.5 0 41 278 110 628.5 0 110 279 138 628.5 0 138 280 120 628.5 0 120 281 124 628.5 0 124 282 122 628.5 0 122 283 139 628.5 0 139 284 81 628.5 0 81 285 134 628.5 0 134 286 80 628.5 0 80 287 96 628.5 0 96 288 101 628.5 0 101 289 98 628.5 0 98 290 66 628.5 0 66 291 71 628.5 0 71 292 119 628.5 0 119 293 154 628.5 0 154 294 178 628.5 0 178 295 194 628.5 0 194 296 157 628.5 0 157 297 158 628.5 0 158 298 189 628.5 0 189 299 190 628.5 0 190 300 264 628.5 0 264 301 441 628.5 0 441 Finished in 3.63 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 71,022 Reads with adapters: 21,191 (29.8%) Reads that were too short: 39 (0.1%) Reads written (passing filters): 21,152 (29.8%) Total basepairs processed: 17,854,223 bp Total written (filtered): 5,777,781 bp (32.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21191 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 3 2 18 13 0.0 3 0 0 9 3 1 19 327 0.0 3 0 253 41 26 7 20 20523 0.0 4 18744 1370 294 89 26 21 55 0.0 4 7 43 2 2 1 22 4 0.0 4 1 1 1 0 1 23 54 0.0 4 1 1 1 28 23 28 3 0.0 4 0 0 0 0 3 32 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 1 79 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 2 0.0 4 0 0 0 0 2 104 1 0.0 4 0 0 0 0 1 108 13 0.0 4 0 0 0 0 13 109 1 0.0 4 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 142 0.0 4 0 0 0 0 142 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 239 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.74 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 21,152 Reads with adapters: 1,454 (6.9%) Reads written (passing filters): 21,152 (100.0%) Total basepairs processed: 5,777,781 bp Total written (filtered): 5,756,714 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1454 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.6% C: 15.3% G: 73.6% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 155 330.5 0 155 4 23 82.6 0 23 5 1 20.7 0 1 6 1 5.2 0 1 9 8 0.1 0 2 6 10 21 0.0 1 0 21 11 870 0.0 1 31 839 12 313 0.0 1 18 295 13 16 0.0 1 0 16 15 6 0.0 1 0 6 24 1 0.0 1 0 1 152 24 0.0 1 18 6 153 3 0.0 1 1 2 171 1 0.0 1 1 191 1 0.0 1 0 1 210 10 0.0 1 9 1