This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.78 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 82,710 (3.2%) Reads that were too short: 7,682 (0.3%) Reads written (passing filters): 75,028 (2.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 18,889,558 bp (2.4%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 82710 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 790 628.5 0 790 7 25595 628.5 0 25595 8 560 628.5 0 560 9 22372 628.5 0 22372 10 37 628.5 0 37 11 28 628.5 0 28 12 25 628.5 0 25 13 25 628.5 0 25 14 29 628.5 0 29 15 29 628.5 0 29 16 37 628.5 0 37 17 32 628.5 0 32 18 22 628.5 0 22 19 16 628.5 0 16 20 25 628.5 0 25 21 21 628.5 0 21 22 34 628.5 0 34 23 34 628.5 0 34 24 18 628.5 0 18 25 42 628.5 0 42 26 69 628.5 0 69 27 50 628.5 0 50 28 126 628.5 0 126 29 71 628.5 0 71 30 87 628.5 0 87 31 58 628.5 0 58 32 20 628.5 0 20 33 25 628.5 0 25 34 40 628.5 0 40 35 39 628.5 0 39 36 24 628.5 0 24 37 23 628.5 0 23 38 27 628.5 0 27 39 34 628.5 0 34 40 29 628.5 0 29 41 10 628.5 0 10 42 32 628.5 0 32 43 32 628.5 0 32 44 31 628.5 0 31 45 27 628.5 0 27 46 22 628.5 0 22 47 18 628.5 0 18 48 13 628.5 0 13 49 40 628.5 0 40 50 44 628.5 0 44 51 45 628.5 0 45 52 39 628.5 0 39 53 49 628.5 0 49 54 32 628.5 0 32 55 37 628.5 0 37 56 33 628.5 0 33 57 34 628.5 0 34 58 42 628.5 0 42 59 33 628.5 0 33 60 41 628.5 0 41 61 42 628.5 0 42 62 35 628.5 0 35 63 37 628.5 0 37 64 41 628.5 0 41 65 28 628.5 0 28 66 38 628.5 0 38 67 56 628.5 0 56 68 52 628.5 0 52 69 434 628.5 0 434 70 210 628.5 0 210 71 427 628.5 0 427 72 34 628.5 0 34 73 37 628.5 0 37 74 33 628.5 0 33 75 61 628.5 0 61 76 40 628.5 0 40 77 55 628.5 0 55 78 48 628.5 0 48 79 75 628.5 0 75 80 35 628.5 0 35 81 41 628.5 0 41 82 39 628.5 0 39 83 46 628.5 0 46 84 35 628.5 0 35 85 34 628.5 0 34 86 50 628.5 0 50 87 51 628.5 0 51 88 43 628.5 0 43 89 34 628.5 0 34 90 47 628.5 0 47 91 44 628.5 0 44 92 48 628.5 0 48 93 42 628.5 0 42 94 38 628.5 0 38 95 39 628.5 0 39 96 81 628.5 0 81 97 46 628.5 0 46 98 58 628.5 0 58 99 48 628.5 0 48 100 48 628.5 0 48 101 49 628.5 0 49 102 39 628.5 0 39 103 26 628.5 0 26 104 44 628.5 0 44 105 47 628.5 0 47 106 37 628.5 0 37 107 51 628.5 0 51 108 48 628.5 0 48 109 34 628.5 0 34 110 43 628.5 0 43 111 53 628.5 0 53 112 56 628.5 0 56 113 50 628.5 0 50 114 220 628.5 0 220 115 356 628.5 0 356 116 77 628.5 0 77 117 40 628.5 0 40 118 42 628.5 0 42 119 36 628.5 0 36 120 25 628.5 0 25 121 35 628.5 0 35 122 39 628.5 0 39 123 53 628.5 0 53 124 68 628.5 0 68 125 75 628.5 0 75 126 106 628.5 0 106 127 109 628.5 0 109 128 132 628.5 0 132 129 88 628.5 0 88 130 65 628.5 0 65 131 69 628.5 0 69 132 76 628.5 0 76 133 281 628.5 0 281 134 124 628.5 0 124 135 88 628.5 0 88 136 123 628.5 0 123 137 138 628.5 0 138 138 732 628.5 0 732 139 1999 628.5 0 1999 140 4015 628.5 0 4015 141 2865 628.5 0 2865 142 365 628.5 0 365 143 368 628.5 0 368 144 525 628.5 0 525 145 498 628.5 0 498 146 66 628.5 0 66 147 186 628.5 0 186 148 300 628.5 0 300 149 1190 628.5 0 1190 150 1945 628.5 0 1945 151 1087 628.5 0 1087 152 154 628.5 0 154 153 96 628.5 0 96 154 46 628.5 0 46 155 67 628.5 0 67 156 31 628.5 0 31 157 39 628.5 0 39 158 28 628.5 0 28 159 34 628.5 0 34 160 27 628.5 0 27 161 24 628.5 0 24 162 23 628.5 0 23 163 18 628.5 0 18 164 24 628.5 0 24 165 31 628.5 0 31 166 25 628.5 0 25 167 23 628.5 0 23 168 31 628.5 0 31 169 28 628.5 0 28 170 37 628.5 0 37 171 13 628.5 0 13 172 36 628.5 0 36 173 38 628.5 0 38 174 25 628.5 0 25 175 30 628.5 0 30 176 33 628.5 0 33 177 29 628.5 0 29 178 23 628.5 0 23 179 37 628.5 0 37 180 27 628.5 0 27 181 30 628.5 0 30 182 26 628.5 0 26 183 39 628.5 0 39 184 34 628.5 0 34 185 52 628.5 0 52 186 36 628.5 0 36 187 38 628.5 0 38 188 46 628.5 0 46 189 63 628.5 0 63 190 86 628.5 0 86 191 123 628.5 0 123 192 40 628.5 0 40 193 23 628.5 0 23 194 50 628.5 0 50 195 24 628.5 0 24 196 22 628.5 0 22 197 29 628.5 0 29 198 23 628.5 0 23 199 39 628.5 0 39 200 24 628.5 0 24 201 44 628.5 0 44 202 27 628.5 0 27 203 33 628.5 0 33 204 26 628.5 0 26 205 23 628.5 0 23 206 28 628.5 0 28 207 34 628.5 0 34 208 24 628.5 0 24 209 159 628.5 0 159 210 62 628.5 0 62 211 38 628.5 0 38 212 40 628.5 0 40 213 33 628.5 0 33 214 38 628.5 0 38 215 33 628.5 0 33 216 18 628.5 0 18 217 32 628.5 0 32 218 25 628.5 0 25 219 24 628.5 0 24 220 25 628.5 0 25 221 21 628.5 0 21 222 31 628.5 0 31 223 28 628.5 0 28 224 26 628.5 0 26 225 24 628.5 0 24 226 22 628.5 0 22 227 21 628.5 0 21 228 18 628.5 0 18 229 32 628.5 0 32 230 26 628.5 0 26 231 30 628.5 0 30 232 29 628.5 0 29 233 61 628.5 0 61 234 93 628.5 0 93 235 91 628.5 0 91 236 54 628.5 0 54 237 27 628.5 0 27 238 54 628.5 0 54 239 30 628.5 0 30 240 28 628.5 0 28 241 44 628.5 0 44 242 45 628.5 0 45 243 43 628.5 0 43 244 49 628.5 0 49 245 44 628.5 0 44 246 40 628.5 0 40 247 45 628.5 0 45 248 71 628.5 0 71 249 83 628.5 0 83 250 189 628.5 0 189 251 35 628.5 0 35 252 43 628.5 0 43 253 317 628.5 0 317 254 484 628.5 0 484 255 734 628.5 0 734 256 93 628.5 0 93 257 27 628.5 0 27 258 50 628.5 0 50 259 26 628.5 0 26 260 41 628.5 0 41 261 36 628.5 0 36 262 45 628.5 0 45 263 26 628.5 0 26 264 43 628.5 0 43 265 28 628.5 0 28 266 33 628.5 0 33 267 39 628.5 0 39 268 54 628.5 0 54 269 75 628.5 0 75 270 95 628.5 0 95 271 49 628.5 0 49 272 78 628.5 0 78 273 147 628.5 0 147 274 201 628.5 0 201 275 115 628.5 0 115 276 68 628.5 0 68 277 40 628.5 0 40 278 75 628.5 0 75 279 69 628.5 0 69 280 43 628.5 0 43 281 60 628.5 0 60 282 72 628.5 0 72 283 107 628.5 0 107 284 29 628.5 0 29 285 82 628.5 0 82 286 69 628.5 0 69 287 76 628.5 0 76 288 94 628.5 0 94 289 100 628.5 0 100 290 54 628.5 0 54 291 70 628.5 0 70 292 85 628.5 0 85 293 69 628.5 0 69 294 81 628.5 0 81 295 191 628.5 0 191 296 143 628.5 0 143 297 118 628.5 0 118 298 108 628.5 0 108 299 140 628.5 0 140 300 166 628.5 0 166 301 438 628.5 0 438 Finished in 3.47 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 75,028 Reads with adapters: 23,318 (31.1%) Reads that were too short: 39 (0.1%) Reads written (passing filters): 23,279 (31.0%) Total basepairs processed: 18,889,558 bp Total written (filtered): 6,371,802 bp (33.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23318 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 4 16 182 0.0 3 0 178 3 1 17 22783 0.0 3 22292 480 8 3 18 64 0.0 3 6 53 5 19 19 0.0 3 2 0 13 4 20 21 0.0 3 0 0 0 21 24 1 0.0 3 1 26 1 0.0 3 0 0 0 1 34 154 0.0 3 0 0 1 153 37 18 0.0 3 0 0 0 18 42 1 0.0 3 0 0 0 1 43 16 0.0 3 0 0 0 16 44 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 146 17 0.0 3 0 0 0 17 147 2 0.0 3 0 0 0 2 148 2 0.0 3 0 0 0 2 149 9 0.0 3 0 0 1 8 162 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 260 1 0.0 3 1 280 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.82 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 23,279 Reads with adapters: 151 (0.6%) Reads written (passing filters): 23,279 (100.0%) Total basepairs processed: 6,371,802 bp Total written (filtered): 6,371,340 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 151 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.8% C: 13.9% G: 54.3% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 142 363.7 0 142 4 9 90.9 0 9