This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.98 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 50,417 (2.0%) Reads that were too short: 20,542 (0.8%) Reads written (passing filters): 29,875 (1.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 7,058,209 bp (0.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 50417 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 267 628.5 0 267 7 221 628.5 0 221 8 6395 628.5 0 6395 9 5485 628.5 0 5485 10 32 628.5 0 32 11 2 628.5 0 2 12 2 628.5 0 2 13 6 628.5 0 6 14 6 628.5 0 6 15 6 628.5 0 6 16 11 628.5 0 11 17 8 628.5 0 8 18 13 628.5 0 13 19 6 628.5 0 6 20 5 628.5 0 5 21 5 628.5 0 5 22 7 628.5 0 7 23 7 628.5 0 7 24 5 628.5 0 5 25 4 628.5 0 4 26 21 628.5 0 21 27 48 628.5 0 48 28 47 628.5 0 47 29 4 628.5 0 4 30 14 628.5 0 14 31 6 628.5 0 6 32 4 628.5 0 4 33 11 628.5 0 11 34 12 628.5 0 12 35 2 628.5 0 2 36 3 628.5 0 3 37 6 628.5 0 6 38 2 628.5 0 2 39 7 628.5 0 7 40 12 628.5 0 12 41 11 628.5 0 11 42 27 628.5 0 27 43 26 628.5 0 26 44 6 628.5 0 6 45 14 628.5 0 14 46 6 628.5 0 6 47 23 628.5 0 23 48 7 628.5 0 7 49 10 628.5 0 10 50 6 628.5 0 6 51 13 628.5 0 13 52 21 628.5 0 21 53 32 628.5 0 32 54 23 628.5 0 23 55 14 628.5 0 14 56 17 628.5 0 17 57 33 628.5 0 33 58 101 628.5 0 101 59 209 628.5 0 209 60 1953 628.5 0 1953 61 996 628.5 0 996 62 1947 628.5 0 1947 63 29 628.5 0 29 64 26 628.5 0 26 65 35 628.5 0 35 66 201 628.5 0 201 67 66 628.5 0 66 68 78 628.5 0 78 69 4 628.5 0 4 70 20 628.5 0 20 71 16 628.5 0 16 72 19 628.5 0 19 73 15 628.5 0 15 74 10 628.5 0 10 75 20 628.5 0 20 76 39 628.5 0 39 77 452 628.5 0 452 78 149 628.5 0 149 79 380 628.5 0 380 80 19 628.5 0 19 81 30 628.5 0 30 82 44 628.5 0 44 83 17 628.5 0 17 84 17 628.5 0 17 85 21 628.5 0 21 86 15 628.5 0 15 87 3 628.5 0 3 88 28 628.5 0 28 89 17 628.5 0 17 90 11 628.5 0 11 91 13 628.5 0 13 92 15 628.5 0 15 93 14 628.5 0 14 94 23 628.5 0 23 95 24 628.5 0 24 96 7 628.5 0 7 97 26 628.5 0 26 98 27 628.5 0 27 99 27 628.5 0 27 100 21 628.5 0 21 101 23 628.5 0 23 102 12 628.5 0 12 103 11 628.5 0 11 104 22 628.5 0 22 105 16 628.5 0 16 106 17 628.5 0 17 107 27 628.5 0 27 108 51 628.5 0 51 109 36 628.5 0 36 110 35 628.5 0 35 111 204 628.5 0 204 112 286 628.5 0 286 113 461 628.5 0 461 114 1039 628.5 0 1039 115 1438 628.5 0 1438 116 1564 628.5 0 1564 117 56 628.5 0 56 118 26 628.5 0 26 119 28 628.5 0 28 120 17 628.5 0 17 121 21 628.5 0 21 122 22 628.5 0 22 123 16 628.5 0 16 124 13 628.5 0 13 125 48 628.5 0 48 126 28 628.5 0 28 127 24 628.5 0 24 128 15 628.5 0 15 129 30 628.5 0 30 130 45 628.5 0 45 131 53 628.5 0 53 132 71 628.5 0 71 133 144 628.5 0 144 134 7 628.5 0 7 135 24 628.5 0 24 136 17 628.5 0 17 137 28 628.5 0 28 138 30 628.5 0 30 139 38 628.5 0 38 140 36 628.5 0 36 141 19 628.5 0 19 142 21 628.5 0 21 143 56 628.5 0 56 144 67 628.5 0 67 145 103 628.5 0 103 146 107 628.5 0 107 147 25 628.5 0 25 148 40 628.5 0 40 149 27 628.5 0 27 150 31 628.5 0 31 151 18 628.5 0 18 152 28 628.5 0 28 153 23 628.5 0 23 154 28 628.5 0 28 155 14 628.5 0 14 156 21 628.5 0 21 157 14 628.5 0 14 158 14 628.5 0 14 159 25 628.5 0 25 160 41 628.5 0 41 161 51 628.5 0 51 162 28 628.5 0 28 163 45 628.5 0 45 164 97 628.5 0 97 165 332 628.5 0 332 166 232 628.5 0 232 167 95 628.5 0 95 168 26 628.5 0 26 169 35 628.5 0 35 170 33 628.5 0 33 171 35 628.5 0 35 172 28 628.5 0 28 173 22 628.5 0 22 174 11 628.5 0 11 175 14 628.5 0 14 176 20 628.5 0 20 177 17 628.5 0 17 178 21 628.5 0 21 179 66 628.5 0 66 180 72 628.5 0 72 181 239 628.5 0 239 182 32 628.5 0 32 183 30 628.5 0 30 184 89 628.5 0 89 185 121 628.5 0 121 186 96 628.5 0 96 187 59 628.5 0 59 188 37 628.5 0 37 189 38 628.5 0 38 190 155 628.5 0 155 191 135 628.5 0 135 192 109 628.5 0 109 193 59 628.5 0 59 194 59 628.5 0 59 195 45 628.5 0 45 196 63 628.5 0 63 197 97 628.5 0 97 198 62 628.5 0 62 199 38 628.5 0 38 200 33 628.5 0 33 201 33 628.5 0 33 202 46 628.5 0 46 203 322 628.5 0 322 204 83 628.5 0 83 205 180 628.5 0 180 206 48 628.5 0 48 207 86 628.5 0 86 208 142 628.5 0 142 209 176 628.5 0 176 210 410 628.5 0 410 211 45 628.5 0 45 212 92 628.5 0 92 213 33 628.5 0 33 214 103 628.5 0 103 215 68 628.5 0 68 216 128 628.5 0 128 217 210 628.5 0 210 218 152 628.5 0 152 219 78 628.5 0 78 220 31 628.5 0 31 221 26 628.5 0 26 222 32 628.5 0 32 223 43 628.5 0 43 224 182 628.5 0 182 225 113 628.5 0 113 226 181 628.5 0 181 227 105 628.5 0 105 228 55 628.5 0 55 229 72 628.5 0 72 230 82 628.5 0 82 231 80 628.5 0 80 232 119 628.5 0 119 233 100 628.5 0 100 234 107 628.5 0 107 235 137 628.5 0 137 236 562 628.5 0 562 237 451 628.5 0 451 238 514 628.5 0 514 239 289 628.5 0 289 240 58 628.5 0 58 241 55 628.5 0 55 242 85 628.5 0 85 243 117 628.5 0 117 244 198 628.5 0 198 245 1830 628.5 0 1830 246 2759 628.5 0 2759 247 2346 628.5 0 2346 248 69 628.5 0 69 249 40 628.5 0 40 250 50 628.5 0 50 251 92 628.5 0 92 252 61 628.5 0 61 253 72 628.5 0 72 254 132 628.5 0 132 255 105 628.5 0 105 256 54 628.5 0 54 257 54 628.5 0 54 258 61 628.5 0 61 259 96 628.5 0 96 260 228 628.5 0 228 261 141 628.5 0 141 262 209 628.5 0 209 263 164 628.5 0 164 264 171 628.5 0 171 265 458 628.5 0 458 266 692 628.5 0 692 267 333 628.5 0 333 268 66 628.5 0 66 269 55 628.5 0 55 270 428 628.5 0 428 271 284 628.5 0 284 272 312 628.5 0 312 273 192 628.5 0 192 274 55 628.5 0 55 275 48 628.5 0 48 276 54 628.5 0 54 277 141 628.5 0 141 278 234 628.5 0 234 279 134 628.5 0 134 280 107 628.5 0 107 281 81 628.5 0 81 282 58 628.5 0 58 283 82 628.5 0 82 284 139 628.5 0 139 285 116 628.5 0 116 286 163 628.5 0 163 287 208 628.5 0 208 288 59 628.5 0 59 289 62 628.5 0 62 290 74 628.5 0 74 291 62 628.5 0 62 292 61 628.5 0 61 293 131 628.5 0 131 294 103 628.5 0 103 295 91 628.5 0 91 296 74 628.5 0 74 297 88 628.5 0 88 298 88 628.5 0 88 299 93 628.5 0 93 300 94 628.5 0 94 301 92 628.5 0 92 Finished in 1.48 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 29,875 Reads with adapters: 6,214 (20.8%) Reads that were too short: 16 (0.1%) Reads written (passing filters): 6,198 (20.7%) Total basepairs processed: 7,058,209 bp Total written (filtered): 1,653,397 bp (23.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6214 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 9 0.0 3 0 0 6 3 19 120 0.0 3 0 101 7 6 6 20 5552 0.0 4 4989 422 97 35 9 21 23 0.0 4 11 9 1 1 1 22 152 0.0 4 135 12 3 0 2 23 35 0.0 4 1 0 0 22 12 25 1 0.0 4 0 0 0 1 26 2 0.0 4 0 0 0 1 1 33 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 119 5 0.0 4 0 0 0 0 5 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 288 0.0 4 0 0 0 0 288 131 1 0.0 4 0 0 0 0 1 138 1 0.0 4 1 140 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 6,198 Reads with adapters: 1,418 (22.9%) Reads written (passing filters): 6,198 (100.0%) Total basepairs processed: 1,653,397 bp Total written (filtered): 1,637,695 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1418 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 8.5% G: 86.2% T: 3.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 96.8 0 2 4 5 24.2 0 5 9 315 0.0 0 44 271 10 968 0.0 1 74 894 11 113 0.0 1 5 108 12 2 0.0 1 0 2 13 2 0.0 1 0 2 150 6 0.0 1 5 1 153 1 0.0 1 0 1 169 1 0.0 1 1 208 1 0.0 1 1 209 1 0.0 1 1 229 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.