This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.90 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 35,971 (1.4%) Reads that were too short: 15,813 (0.6%) Reads written (passing filters): 20,158 (0.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 5,174,783 bp (0.7%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 35971 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 287 628.5 0 287 7 185 628.5 0 185 8 6313 628.5 0 6313 9 6347 628.5 0 6347 10 12 628.5 0 12 11 1 628.5 0 1 12 2 628.5 0 2 13 3 628.5 0 3 14 1 628.5 0 1 15 1 628.5 0 1 16 1 628.5 0 1 21 2 628.5 0 2 22 2 628.5 0 2 23 3 628.5 0 3 24 3 628.5 0 3 25 1 628.5 0 1 26 4 628.5 0 4 27 6 628.5 0 6 28 4 628.5 0 4 29 1 628.5 0 1 30 7 628.5 0 7 31 1 628.5 0 1 32 3 628.5 0 3 33 7 628.5 0 7 34 13 628.5 0 13 35 4 628.5 0 4 36 2 628.5 0 2 37 2 628.5 0 2 38 1 628.5 0 1 39 6 628.5 0 6 40 5 628.5 0 5 41 13 628.5 0 13 42 7 628.5 0 7 43 6 628.5 0 6 44 9 628.5 0 9 45 3 628.5 0 3 46 6 628.5 0 6 47 13 628.5 0 13 48 5 628.5 0 5 49 4 628.5 0 4 50 6 628.5 0 6 51 10 628.5 0 10 52 8 628.5 0 8 53 9 628.5 0 9 54 7 628.5 0 7 55 6 628.5 0 6 56 9 628.5 0 9 57 14 628.5 0 14 58 45 628.5 0 45 59 75 628.5 0 75 60 623 628.5 0 623 61 246 628.5 0 246 62 590 628.5 0 590 63 8 628.5 0 8 64 7 628.5 0 7 65 10 628.5 0 10 66 137 628.5 0 137 67 31 628.5 0 31 68 28 628.5 0 28 69 4 628.5 0 4 70 11 628.5 0 11 71 3 628.5 0 3 72 11 628.5 0 11 73 6 628.5 0 6 74 5 628.5 0 5 75 20 628.5 0 20 76 20 628.5 0 20 77 230 628.5 0 230 78 77 628.5 0 77 79 191 628.5 0 191 80 12 628.5 0 12 81 20 628.5 0 20 82 13 628.5 0 13 83 8 628.5 0 8 84 14 628.5 0 14 85 10 628.5 0 10 86 12 628.5 0 12 87 2 628.5 0 2 88 24 628.5 0 24 89 11 628.5 0 11 90 32 628.5 0 32 91 6 628.5 0 6 92 5 628.5 0 5 93 15 628.5 0 15 94 10 628.5 0 10 95 9 628.5 0 9 96 8 628.5 0 8 97 23 628.5 0 23 98 24 628.5 0 24 99 13 628.5 0 13 100 9 628.5 0 9 101 24 628.5 0 24 102 8 628.5 0 8 103 6 628.5 0 6 104 14 628.5 0 14 105 15 628.5 0 15 106 9 628.5 0 9 107 17 628.5 0 17 108 13 628.5 0 13 109 10 628.5 0 10 110 17 628.5 0 17 111 92 628.5 0 92 112 106 628.5 0 106 113 146 628.5 0 146 114 318 628.5 0 318 115 425 628.5 0 425 116 622 628.5 0 622 117 12 628.5 0 12 118 7 628.5 0 7 119 10 628.5 0 10 120 8 628.5 0 8 121 11 628.5 0 11 122 27 628.5 0 27 123 13 628.5 0 13 124 13 628.5 0 13 125 23 628.5 0 23 126 12 628.5 0 12 127 15 628.5 0 15 128 5 628.5 0 5 129 14 628.5 0 14 130 15 628.5 0 15 131 14 628.5 0 14 132 27 628.5 0 27 133 48 628.5 0 48 134 7 628.5 0 7 135 9 628.5 0 9 136 15 628.5 0 15 137 12 628.5 0 12 138 8 628.5 0 8 139 29 628.5 0 29 140 39 628.5 0 39 141 10 628.5 0 10 142 5 628.5 0 5 143 15 628.5 0 15 144 23 628.5 0 23 145 44 628.5 0 44 146 109 628.5 0 109 147 14 628.5 0 14 148 17 628.5 0 17 149 10 628.5 0 10 150 14 628.5 0 14 151 9 628.5 0 9 152 14 628.5 0 14 153 9 628.5 0 9 154 8 628.5 0 8 155 8 628.5 0 8 156 15 628.5 0 15 157 12 628.5 0 12 158 8 628.5 0 8 159 10 628.5 0 10 160 15 628.5 0 15 161 6 628.5 0 6 162 7 628.5 0 7 163 16 628.5 0 16 164 28 628.5 0 28 165 177 628.5 0 177 166 77 628.5 0 77 167 42 628.5 0 42 168 13 628.5 0 13 169 20 628.5 0 20 170 8 628.5 0 8 171 26 628.5 0 26 172 18 628.5 0 18 173 16 628.5 0 16 174 5 628.5 0 5 175 10 628.5 0 10 176 7 628.5 0 7 177 11 628.5 0 11 178 13 628.5 0 13 179 31 628.5 0 31 180 54 628.5 0 54 181 335 628.5 0 335 182 11 628.5 0 11 183 14 628.5 0 14 184 15 628.5 0 15 185 33 628.5 0 33 186 34 628.5 0 34 187 18 628.5 0 18 188 13 628.5 0 13 189 22 628.5 0 22 190 91 628.5 0 91 191 69 628.5 0 69 192 49 628.5 0 49 193 66 628.5 0 66 194 70 628.5 0 70 195 21 628.5 0 21 196 17 628.5 0 17 197 19 628.5 0 19 198 16 628.5 0 16 199 11 628.5 0 11 200 15 628.5 0 15 201 7 628.5 0 7 202 25 628.5 0 25 203 162 628.5 0 162 204 28 628.5 0 28 205 110 628.5 0 110 206 16 628.5 0 16 207 33 628.5 0 33 208 61 628.5 0 61 209 84 628.5 0 84 210 189 628.5 0 189 211 22 628.5 0 22 212 88 628.5 0 88 213 27 628.5 0 27 214 136 628.5 0 136 215 22 628.5 0 22 216 35 628.5 0 35 217 42 628.5 0 42 218 37 628.5 0 37 219 32 628.5 0 32 220 20 628.5 0 20 221 17 628.5 0 17 222 14 628.5 0 14 223 20 628.5 0 20 224 55 628.5 0 55 225 31 628.5 0 31 226 51 628.5 0 51 227 20 628.5 0 20 228 26 628.5 0 26 229 27 628.5 0 27 230 35 628.5 0 35 231 55 628.5 0 55 232 103 628.5 0 103 233 44 628.5 0 44 234 45 628.5 0 45 235 82 628.5 0 82 236 200 628.5 0 200 237 185 628.5 0 185 238 240 628.5 0 240 239 121 628.5 0 121 240 47 628.5 0 47 241 38 628.5 0 38 242 64 628.5 0 64 243 102 628.5 0 102 244 124 628.5 0 124 245 2408 628.5 0 2408 246 3792 628.5 0 3792 247 2941 628.5 0 2941 248 32 628.5 0 32 249 9 628.5 0 9 250 31 628.5 0 31 251 84 628.5 0 84 252 28 628.5 0 28 253 23 628.5 0 23 254 25 628.5 0 25 255 29 628.5 0 29 256 27 628.5 0 27 257 21 628.5 0 21 258 19 628.5 0 19 259 25 628.5 0 25 260 127 628.5 0 127 261 84 628.5 0 84 262 147 628.5 0 147 263 45 628.5 0 45 264 75 628.5 0 75 265 289 628.5 0 289 266 576 628.5 0 576 267 344 628.5 0 344 268 27 628.5 0 27 269 18 628.5 0 18 270 207 628.5 0 207 271 96 628.5 0 96 272 90 628.5 0 90 273 80 628.5 0 80 274 47 628.5 0 47 275 53 628.5 0 53 276 31 628.5 0 31 277 61 628.5 0 61 278 106 628.5 0 106 279 23 628.5 0 23 280 27 628.5 0 27 281 23 628.5 0 23 282 40 628.5 0 40 283 24 628.5 0 24 284 38 628.5 0 38 285 33 628.5 0 33 286 61 628.5 0 61 287 76 628.5 0 76 288 25 628.5 0 25 289 28 628.5 0 28 290 50 628.5 0 50 291 26 628.5 0 26 292 29 628.5 0 29 293 99 628.5 0 99 294 38 628.5 0 38 295 21 628.5 0 21 296 26 628.5 0 26 297 42 628.5 0 42 298 37 628.5 0 37 299 50 628.5 0 50 300 42 628.5 0 42 301 43 628.5 0 43 Finished in 0.96 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 20,158 Reads with adapters: 6,248 (31.0%) Reads that were too short: 27 (0.1%) Reads written (passing filters): 6,221 (30.9%) Total basepairs processed: 5,174,783 bp Total written (filtered): 1,714,033 bp (33.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6248 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 53 0.0 3 0 51 1 1 17 6106 0.0 3 6003 103 18 26 0.0 3 15 11 19 6 0.0 3 0 0 5 1 20 6 0.0 3 0 0 0 6 28 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 77 2 0.0 3 0 0 0 2 78 7 0.0 3 0 0 0 7 81 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 148 14 0.0 3 0 0 0 14 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 160 3 0.0 3 0 0 1 2 169 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 6,221 Reads with adapters: 31 (0.5%) Reads written (passing filters): 6,221 (100.0%) Total basepairs processed: 1,714,033 bp Total written (filtered): 1,713,936 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 31 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.1% C: 0.0% G: 0.0% T: 12.9% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 27 97.2 0 27 4 4 24.3 0 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.