This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.37 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 224,427 (8.7%) Reads that were too short: 86,022 (3.3%) Reads written (passing filters): 138,405 (5.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 36,817,561 bp (4.8%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 224427 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 983 628.5 0 983 7 26610 628.5 0 26610 8 22182 628.5 0 22182 9 780 628.5 0 780 10 1679 628.5 0 1679 11 56494 628.5 0 56494 12 125 628.5 0 125 13 97 628.5 0 97 14 75 628.5 0 75 15 98 628.5 0 98 16 114 628.5 0 114 17 112 628.5 0 112 18 56 628.5 0 56 19 49 628.5 0 49 20 75 628.5 0 75 21 60 628.5 0 60 22 52 628.5 0 52 23 82 628.5 0 82 24 70 628.5 0 70 25 75 628.5 0 75 26 58 628.5 0 58 27 64 628.5 0 64 28 55 628.5 0 55 29 67 628.5 0 67 30 80 628.5 0 80 31 79 628.5 0 79 32 76 628.5 0 76 33 69 628.5 0 69 34 73 628.5 0 73 35 79 628.5 0 79 36 73 628.5 0 73 37 75 628.5 0 75 38 80 628.5 0 80 39 71 628.5 0 71 40 83 628.5 0 83 41 65 628.5 0 65 42 61 628.5 0 61 43 122 628.5 0 122 44 63 628.5 0 63 45 93 628.5 0 93 46 81 628.5 0 81 47 60 628.5 0 60 48 71 628.5 0 71 49 63 628.5 0 63 50 83 628.5 0 83 51 67 628.5 0 67 52 116 628.5 0 116 53 160 628.5 0 160 54 223 628.5 0 223 55 127 628.5 0 127 56 83 628.5 0 83 57 98 628.5 0 98 58 88 628.5 0 88 59 124 628.5 0 124 60 141 628.5 0 141 61 84 628.5 0 84 62 88 628.5 0 88 63 90 628.5 0 90 64 115 628.5 0 115 65 124 628.5 0 124 66 77 628.5 0 77 67 94 628.5 0 94 68 107 628.5 0 107 69 89 628.5 0 89 70 86 628.5 0 86 71 114 628.5 0 114 72 330 628.5 0 330 73 200 628.5 0 200 74 259 628.5 0 259 75 105 628.5 0 105 76 54 628.5 0 54 77 88 628.5 0 88 78 102 628.5 0 102 79 103 628.5 0 103 80 90 628.5 0 90 81 110 628.5 0 110 82 97 628.5 0 97 83 86 628.5 0 86 84 101 628.5 0 101 85 96 628.5 0 96 86 101 628.5 0 101 87 89 628.5 0 89 88 89 628.5 0 89 89 92 628.5 0 92 90 83 628.5 0 83 91 93 628.5 0 93 92 67 628.5 0 67 93 86 628.5 0 86 94 75 628.5 0 75 95 89 628.5 0 89 96 81 628.5 0 81 97 84 628.5 0 84 98 94 628.5 0 94 99 86 628.5 0 86 100 94 628.5 0 94 101 80 628.5 0 80 102 162 628.5 0 162 103 211 628.5 0 211 104 207 628.5 0 207 105 121 628.5 0 121 106 130 628.5 0 130 107 130 628.5 0 130 108 91 628.5 0 91 109 103 628.5 0 103 110 111 628.5 0 111 111 124 628.5 0 124 112 110 628.5 0 110 113 103 628.5 0 103 114 98 628.5 0 98 115 100 628.5 0 100 116 109 628.5 0 109 117 99 628.5 0 99 118 89 628.5 0 89 119 86 628.5 0 86 120 104 628.5 0 104 121 130 628.5 0 130 122 150 628.5 0 150 123 138 628.5 0 138 124 272 628.5 0 272 125 277 628.5 0 277 126 291 628.5 0 291 127 123 628.5 0 123 128 101 628.5 0 101 129 120 628.5 0 120 130 136 628.5 0 136 131 123 628.5 0 123 132 388 628.5 0 388 133 329 628.5 0 329 134 170 628.5 0 170 135 239 628.5 0 239 136 96 628.5 0 96 137 162 628.5 0 162 138 140 628.5 0 140 139 139 628.5 0 139 140 145 628.5 0 145 141 119 628.5 0 119 142 111 628.5 0 111 143 109 628.5 0 109 144 142 628.5 0 142 145 180 628.5 0 180 146 228 628.5 0 228 147 534 628.5 0 534 148 265 628.5 0 265 149 393 628.5 0 393 150 205 628.5 0 205 151 170 628.5 0 170 152 164 628.5 0 164 153 163 628.5 0 163 154 156 628.5 0 156 155 127 628.5 0 127 156 292 628.5 0 292 157 250 628.5 0 250 158 338 628.5 0 338 159 216 628.5 0 216 160 207 628.5 0 207 161 213 628.5 0 213 162 243 628.5 0 243 163 199 628.5 0 199 164 162 628.5 0 162 165 174 628.5 0 174 166 215 628.5 0 215 167 217 628.5 0 217 168 215 628.5 0 215 169 1288 628.5 0 1288 170 543 628.5 0 543 171 852 628.5 0 852 172 374 628.5 0 374 173 285 628.5 0 285 174 298 628.5 0 298 175 175 628.5 0 175 176 157 628.5 0 157 177 134 628.5 0 134 178 126 628.5 0 126 179 203 628.5 0 203 180 227 628.5 0 227 181 350 628.5 0 350 182 286 628.5 0 286 183 233 628.5 0 233 184 204 628.5 0 204 185 239 628.5 0 239 186 184 628.5 0 184 187 401 628.5 0 401 188 279 628.5 0 279 189 398 628.5 0 398 190 105 628.5 0 105 191 145 628.5 0 145 192 175 628.5 0 175 193 173 628.5 0 173 194 185 628.5 0 185 195 181 628.5 0 181 196 158 628.5 0 158 197 158 628.5 0 158 198 163 628.5 0 163 199 198 628.5 0 198 200 187 628.5 0 187 201 166 628.5 0 166 202 160 628.5 0 160 203 431 628.5 0 431 204 293 628.5 0 293 205 403 628.5 0 403 206 170 628.5 0 170 207 114 628.5 0 114 208 149 628.5 0 149 209 195 628.5 0 195 210 140 628.5 0 140 211 174 628.5 0 174 212 196 628.5 0 196 213 298 628.5 0 298 214 995 628.5 0 995 215 960 628.5 0 960 216 1208 628.5 0 1208 217 818 628.5 0 818 218 309 628.5 0 309 219 432 628.5 0 432 220 248 628.5 0 248 221 266 628.5 0 266 222 281 628.5 0 281 223 337 628.5 0 337 224 459 628.5 0 459 225 922 628.5 0 922 226 4756 628.5 0 4756 227 7499 628.5 0 7499 228 8839 628.5 0 8839 229 7695 628.5 0 7695 230 1533 628.5 0 1533 231 1243 628.5 0 1243 232 915 628.5 0 915 233 612 628.5 0 612 234 172 628.5 0 172 235 139 628.5 0 139 236 179 628.5 0 179 237 334 628.5 0 334 238 350 628.5 0 350 239 419 628.5 0 419 240 260 628.5 0 260 241 139 628.5 0 139 242 132 628.5 0 132 243 120 628.5 0 120 244 181 628.5 0 181 245 236 628.5 0 236 246 198 628.5 0 198 247 217 628.5 0 217 248 360 628.5 0 360 249 285 628.5 0 285 250 217 628.5 0 217 251 151 628.5 0 151 252 128 628.5 0 128 253 151 628.5 0 151 254 227 628.5 0 227 255 288 628.5 0 288 256 389 628.5 0 389 257 587 628.5 0 587 258 454 628.5 0 454 259 468 628.5 0 468 260 362 628.5 0 362 261 398 628.5 0 398 262 335 628.5 0 335 263 531 628.5 0 531 264 2331 628.5 0 2331 265 3739 628.5 0 3739 266 5343 628.5 0 5343 267 4429 628.5 0 4429 268 2627 628.5 0 2627 269 417 628.5 0 417 270 358 628.5 0 358 271 307 628.5 0 307 272 343 628.5 0 343 273 595 628.5 0 595 274 607 628.5 0 607 275 586 628.5 0 586 276 513 628.5 0 513 277 283 628.5 0 283 278 297 628.5 0 297 279 455 628.5 0 455 280 464 628.5 0 464 281 468 628.5 0 468 282 369 628.5 0 369 283 220 628.5 0 220 284 237 628.5 0 237 285 322 628.5 0 322 286 370 628.5 0 370 287 436 628.5 0 436 288 355 628.5 0 355 289 243 628.5 0 243 290 309 628.5 0 309 291 1710 628.5 0 1710 292 2182 628.5 0 2182 293 1786 628.5 0 1786 294 342 628.5 0 342 295 189 628.5 0 189 296 161 628.5 0 161 297 137 628.5 0 137 298 142 628.5 0 142 299 177 628.5 0 177 300 212 628.5 0 212 301 504 628.5 0 504 Finished in 7.26 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 138,405 Reads with adapters: 28,521 (20.6%) Reads that were too short: 86 (0.1%) Reads written (passing filters): 28,435 (20.5%) Total basepairs processed: 36,817,561 bp Total written (filtered): 7,100,828 bp (19.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28521 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 1 5 18 26 0.0 3 0 0 14 4 8 19 461 0.0 3 0 343 54 45 19 20 21616 0.0 4 19456 1622 388 116 34 21 66 0.0 4 6 51 5 1 3 22 9 0.0 4 0 0 5 0 4 23 103 0.0 4 0 0 0 70 33 24 1 0.0 4 0 0 0 0 1 26 2 0.0 4 0 0 0 0 2 32 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 1 36 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 58 2 0.0 4 1 0 0 0 1 61 2 0.0 4 0 0 1 0 1 64 1 0.0 4 0 0 0 0 1 71 5 0.0 4 0 0 0 0 5 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 77 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 90 2 0.0 4 1 0 0 0 1 91 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 119 1 0.0 4 1 120 1 0.0 4 0 1 123 13 0.0 4 0 0 0 0 13 124 53 0.0 4 0 0 0 0 53 125 5780 0.0 4 0 0 0 0 5780 126 19 0.0 4 0 0 0 0 19 127 223 0.0 4 0 0 0 0 223 128 30 0.0 4 0 0 0 0 30 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 200 2 0.0 4 0 0 0 0 2 205 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 224 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 227 2 0.0 4 0 0 0 0 2 228 4 0.0 4 0 0 0 0 4 229 6 0.0 4 0 0 0 1 5 230 1 0.0 4 0 0 0 0 1 233 3 0.0 4 0 0 0 0 3 234 2 0.0 4 0 0 0 0 2 235 4 0.0 4 0 0 0 0 4 236 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 280 2 0.0 4 0 0 0 0 2 282 8 0.0 4 0 0 0 0 8 283 3 0.0 4 0 0 0 0 3 287 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.98 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 28,435 Reads with adapters: 4,436 (15.6%) Reads written (passing filters): 28,435 (100.0%) Total basepairs processed: 7,100,828 bp Total written (filtered): 7,051,428 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4436 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.1% C: 11.2% G: 83.0% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 43 444.3 0 43 4 16 111.1 0 16 8 2 0.4 0 2 9 39 0.1 0 5 34 10 815 0.0 1 60 755 11 3451 0.0 1 164 3287 12 32 0.0 1 1 31 13 3 0.0 1 0 3 14 7 0.0 1 0 7 18 1 0.0 1 0 1 19 15 0.0 1 5 10 20 1 0.0 1 1 70 1 0.0 1 1 151 5 0.0 1 2 3 209 4 0.0 1 2 2 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.