This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.62 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 171,762 (6.7%) Reads that were too short: 39,011 (1.5%) Reads written (passing filters): 132,751 (5.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 36,258,954 bp (4.7%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 171762 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 689 628.5 0 689 7 23820 628.5 0 23820 8 27776 628.5 0 27776 9 504 628.5 0 504 10 1012 628.5 0 1012 11 56657 628.5 0 56657 12 123 628.5 0 123 13 80 628.5 0 80 14 100 628.5 0 100 15 84 628.5 0 84 16 76 628.5 0 76 17 84 628.5 0 84 18 71 628.5 0 71 19 77 628.5 0 77 20 83 628.5 0 83 21 52 628.5 0 52 22 91 628.5 0 91 23 64 628.5 0 64 24 99 628.5 0 99 25 86 628.5 0 86 26 75 628.5 0 75 27 74 628.5 0 74 28 71 628.5 0 71 29 82 628.5 0 82 30 59 628.5 0 59 31 118 628.5 0 118 32 102 628.5 0 102 33 69 628.5 0 69 34 90 628.5 0 90 35 98 628.5 0 98 36 84 628.5 0 84 37 79 628.5 0 79 38 102 628.5 0 102 39 72 628.5 0 72 40 58 628.5 0 58 41 60 628.5 0 60 42 59 628.5 0 59 43 54 628.5 0 54 44 62 628.5 0 62 45 96 628.5 0 96 46 74 628.5 0 74 47 65 628.5 0 65 48 66 628.5 0 66 49 77 628.5 0 77 50 74 628.5 0 74 51 70 628.5 0 70 52 107 628.5 0 107 53 117 628.5 0 117 54 92 628.5 0 92 55 53 628.5 0 53 56 95 628.5 0 95 57 88 628.5 0 88 58 67 628.5 0 67 59 89 628.5 0 89 60 118 628.5 0 118 61 90 628.5 0 90 62 76 628.5 0 76 63 78 628.5 0 78 64 128 628.5 0 128 65 94 628.5 0 94 66 101 628.5 0 101 67 71 628.5 0 71 68 81 628.5 0 81 69 71 628.5 0 71 70 93 628.5 0 93 71 101 628.5 0 101 72 226 628.5 0 226 73 111 628.5 0 111 74 164 628.5 0 164 75 106 628.5 0 106 76 66 628.5 0 66 77 82 628.5 0 82 78 114 628.5 0 114 79 107 628.5 0 107 80 117 628.5 0 117 81 148 628.5 0 148 82 123 628.5 0 123 83 144 628.5 0 144 84 106 628.5 0 106 85 109 628.5 0 109 86 129 628.5 0 129 87 117 628.5 0 117 88 108 628.5 0 108 89 80 628.5 0 80 90 98 628.5 0 98 91 85 628.5 0 85 92 90 628.5 0 90 93 96 628.5 0 96 94 78 628.5 0 78 95 81 628.5 0 81 96 94 628.5 0 94 97 99 628.5 0 99 98 90 628.5 0 90 99 138 628.5 0 138 100 110 628.5 0 110 101 78 628.5 0 78 102 100 628.5 0 100 103 140 628.5 0 140 104 118 628.5 0 118 105 87 628.5 0 87 106 101 628.5 0 101 107 113 628.5 0 113 108 94 628.5 0 94 109 87 628.5 0 87 110 112 628.5 0 112 111 75 628.5 0 75 112 82 628.5 0 82 113 82 628.5 0 82 114 83 628.5 0 83 115 94 628.5 0 94 116 129 628.5 0 129 117 103 628.5 0 103 118 96 628.5 0 96 119 90 628.5 0 90 120 96 628.5 0 96 121 121 628.5 0 121 122 101 628.5 0 101 123 99 628.5 0 99 124 110 628.5 0 110 125 136 628.5 0 136 126 140 628.5 0 140 127 104 628.5 0 104 128 87 628.5 0 87 129 111 628.5 0 111 130 107 628.5 0 107 131 122 628.5 0 122 132 320 628.5 0 320 133 161 628.5 0 161 134 118 628.5 0 118 135 111 628.5 0 111 136 129 628.5 0 129 137 134 628.5 0 134 138 118 628.5 0 118 139 131 628.5 0 131 140 104 628.5 0 104 141 82 628.5 0 82 142 105 628.5 0 105 143 78 628.5 0 78 144 100 628.5 0 100 145 127 628.5 0 127 146 158 628.5 0 158 147 370 628.5 0 370 148 141 628.5 0 141 149 196 628.5 0 196 150 109 628.5 0 109 151 100 628.5 0 100 152 114 628.5 0 114 153 101 628.5 0 101 154 104 628.5 0 104 155 87 628.5 0 87 156 302 628.5 0 302 157 179 628.5 0 179 158 351 628.5 0 351 159 98 628.5 0 98 160 133 628.5 0 133 161 83 628.5 0 83 162 125 628.5 0 125 163 117 628.5 0 117 164 112 628.5 0 112 165 116 628.5 0 116 166 137 628.5 0 137 167 123 628.5 0 123 168 125 628.5 0 125 169 852 628.5 0 852 170 239 628.5 0 239 171 432 628.5 0 432 172 187 628.5 0 187 173 121 628.5 0 121 174 155 628.5 0 155 175 116 628.5 0 116 176 98 628.5 0 98 177 120 628.5 0 120 178 116 628.5 0 116 179 103 628.5 0 103 180 128 628.5 0 128 181 115 628.5 0 115 182 125 628.5 0 125 183 122 628.5 0 122 184 118 628.5 0 118 185 130 628.5 0 130 186 116 628.5 0 116 187 184 628.5 0 184 188 223 628.5 0 223 189 233 628.5 0 233 190 144 628.5 0 144 191 103 628.5 0 103 192 141 628.5 0 141 193 155 628.5 0 155 194 110 628.5 0 110 195 174 628.5 0 174 196 141 628.5 0 141 197 135 628.5 0 135 198 121 628.5 0 121 199 126 628.5 0 126 200 118 628.5 0 118 201 109 628.5 0 109 202 131 628.5 0 131 203 214 628.5 0 214 204 151 628.5 0 151 205 169 628.5 0 169 206 110 628.5 0 110 207 112 628.5 0 112 208 95 628.5 0 95 209 106 628.5 0 106 210 106 628.5 0 106 211 127 628.5 0 127 212 128 628.5 0 128 213 171 628.5 0 171 214 538 628.5 0 538 215 481 628.5 0 481 216 637 628.5 0 637 217 353 628.5 0 353 218 153 628.5 0 153 219 165 628.5 0 165 220 129 628.5 0 129 221 120 628.5 0 120 222 135 628.5 0 135 223 177 628.5 0 177 224 229 628.5 0 229 225 366 628.5 0 366 226 2213 628.5 0 2213 227 3475 628.5 0 3475 228 3065 628.5 0 3065 229 4148 628.5 0 4148 230 1134 628.5 0 1134 231 1090 628.5 0 1090 232 857 628.5 0 857 233 690 628.5 0 690 234 110 628.5 0 110 235 83 628.5 0 83 236 107 628.5 0 107 237 153 628.5 0 153 238 178 628.5 0 178 239 195 628.5 0 195 240 127 628.5 0 127 241 113 628.5 0 113 242 105 628.5 0 105 243 90 628.5 0 90 244 105 628.5 0 105 245 175 628.5 0 175 246 143 628.5 0 143 247 133 628.5 0 133 248 218 628.5 0 218 249 182 628.5 0 182 250 153 628.5 0 153 251 130 628.5 0 130 252 115 628.5 0 115 253 94 628.5 0 94 254 129 628.5 0 129 255 126 628.5 0 126 256 150 628.5 0 150 257 181 628.5 0 181 258 143 628.5 0 143 259 178 628.5 0 178 260 165 628.5 0 165 261 240 628.5 0 240 262 181 628.5 0 181 263 208 628.5 0 208 264 792 628.5 0 792 265 1179 628.5 0 1179 266 1696 628.5 0 1696 267 1357 628.5 0 1357 268 866 628.5 0 866 269 180 628.5 0 180 270 188 628.5 0 188 271 151 628.5 0 151 272 129 628.5 0 129 273 222 628.5 0 222 274 274 628.5 0 274 275 245 628.5 0 245 276 303 628.5 0 303 277 154 628.5 0 154 278 169 628.5 0 169 279 219 628.5 0 219 280 209 628.5 0 209 281 203 628.5 0 203 282 194 628.5 0 194 283 181 628.5 0 181 284 149 628.5 0 149 285 128 628.5 0 128 286 176 628.5 0 176 287 195 628.5 0 195 288 170 628.5 0 170 289 129 628.5 0 129 290 148 628.5 0 148 291 616 628.5 0 616 292 643 628.5 0 643 293 524 628.5 0 524 294 163 628.5 0 163 295 126 628.5 0 126 296 108 628.5 0 108 297 108 628.5 0 108 298 100 628.5 0 100 299 125 628.5 0 125 300 120 628.5 0 120 301 187 628.5 0 187 Finished in 5.96 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 132,751 Reads with adapters: 23,806 (17.9%) Reads that were too short: 146 (0.1%) Reads written (passing filters): 23,660 (17.8%) Total basepairs processed: 36,258,954 bp Total written (filtered): 6,457,050 bp (17.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23806 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 150 0.0 3 0 147 3 17 22647 0.0 3 22160 475 11 1 18 55 0.0 3 13 40 1 1 19 16 0.0 3 0 0 15 1 20 12 0.0 3 0 0 0 12 21 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 1 38 1 0.0 3 1 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 67 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 1 80 2 0.0 3 2 81 1 0.0 3 1 88 1 0.0 3 0 0 1 91 4 0.0 3 0 0 0 4 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 110 3 0.0 3 0 0 0 3 113 1 0.0 3 0 0 0 1 116 1 0.0 3 1 117 4 0.0 3 0 2 2 118 4 0.0 3 0 2 1 1 126 7 0.0 3 0 0 1 6 127 561 0.0 3 0 0 1 560 128 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 11 0.0 3 0 0 0 11 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 29 0.0 3 0 0 1 28 163 9 0.0 3 0 0 0 9 164 17 0.0 3 0 0 0 17 165 5 0.0 3 0 0 0 5 167 5 0.0 3 0 0 0 5 168 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 4 0.0 3 0 0 0 4 175 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 40 0.0 3 0 0 0 40 183 27 0.0 3 0 0 1 26 185 10 0.0 3 0 0 0 10 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 70 0.0 3 0 0 2 68 218 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 2 0.0 3 0 0 0 2 245 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 267 10 0.0 3 0 0 0 10 268 1 0.0 3 1 269 1 0.0 3 0 0 0 1 274 4 0.0 3 0 0 0 4 275 3 0.0 3 0 0 0 3 276 3 0.0 3 0 0 0 3 277 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 284 7 0.0 3 0 0 0 7 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.81 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 23,660 Reads with adapters: 40 (0.2%) Reads written (passing filters): 23,660 (100.0%) Total basepairs processed: 6,457,050 bp Total written (filtered): 6,456,928 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 40 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.0% C: 52.5% G: 2.5% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 38 369.7 0 38 4 2 92.4 0 2