This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.33 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 54,734 (2.1%) Reads that were too short: 8,814 (0.3%) Reads written (passing filters): 45,920 (1.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,359,568 bp (1.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 54734 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 713 628.5 0 713 7 12884 628.5 0 12884 8 491 628.5 0 491 9 12989 628.5 0 12989 10 11 628.5 0 11 11 5 628.5 0 5 12 3 628.5 0 3 13 5 628.5 0 5 14 3 628.5 0 3 15 5 628.5 0 5 16 1 628.5 0 1 17 2 628.5 0 2 18 2 628.5 0 2 19 3 628.5 0 3 20 4 628.5 0 4 21 10 628.5 0 10 22 15 628.5 0 15 23 35 628.5 0 35 24 5 628.5 0 5 25 74 628.5 0 74 26 11 628.5 0 11 27 19 628.5 0 19 28 3 628.5 0 3 29 4 628.5 0 4 30 7 628.5 0 7 31 5 628.5 0 5 32 8 628.5 0 8 33 19 628.5 0 19 34 103 628.5 0 103 35 248 628.5 0 248 36 6 628.5 0 6 37 7 628.5 0 7 38 6 628.5 0 6 39 4 628.5 0 4 40 13 628.5 0 13 41 7 628.5 0 7 42 15 628.5 0 15 43 3 628.5 0 3 44 3 628.5 0 3 45 2 628.5 0 2 46 3 628.5 0 3 48 8 628.5 0 8 49 4 628.5 0 4 50 6 628.5 0 6 51 4 628.5 0 4 52 7 628.5 0 7 53 3 628.5 0 3 54 2 628.5 0 2 55 2 628.5 0 2 56 4 628.5 0 4 57 3 628.5 0 3 58 11 628.5 0 11 59 5 628.5 0 5 60 7 628.5 0 7 61 6 628.5 0 6 62 10 628.5 0 10 63 2 628.5 0 2 64 5 628.5 0 5 65 7 628.5 0 7 66 12 628.5 0 12 67 5 628.5 0 5 68 9 628.5 0 9 69 7 628.5 0 7 70 8 628.5 0 8 71 5 628.5 0 5 72 3 628.5 0 3 73 10 628.5 0 10 74 31 628.5 0 31 75 10 628.5 0 10 76 18 628.5 0 18 77 25 628.5 0 25 78 54 628.5 0 54 79 54 628.5 0 54 80 16 628.5 0 16 81 35 628.5 0 35 82 19 628.5 0 19 83 28 628.5 0 28 84 13 628.5 0 13 85 17 628.5 0 17 86 32 628.5 0 32 87 24 628.5 0 24 88 11 628.5 0 11 89 34 628.5 0 34 90 28 628.5 0 28 91 14 628.5 0 14 92 10 628.5 0 10 93 32 628.5 0 32 94 34 628.5 0 34 95 26 628.5 0 26 96 54 628.5 0 54 97 105 628.5 0 105 98 114 628.5 0 114 99 923 628.5 0 923 100 862 628.5 0 862 101 1367 628.5 0 1367 102 59 628.5 0 59 103 21 628.5 0 21 104 23 628.5 0 23 105 16 628.5 0 16 106 33 628.5 0 33 107 45 628.5 0 45 108 50 628.5 0 50 109 64 628.5 0 64 110 93 628.5 0 93 111 886 628.5 0 886 112 1494 628.5 0 1494 113 2113 628.5 0 2113 114 458 628.5 0 458 115 1106 628.5 0 1106 116 16 628.5 0 16 117 15 628.5 0 15 118 12 628.5 0 12 119 29 628.5 0 29 120 16 628.5 0 16 121 12 628.5 0 12 122 14 628.5 0 14 123 17 628.5 0 17 124 17 628.5 0 17 125 15 628.5 0 15 126 25 628.5 0 25 127 17 628.5 0 17 128 29 628.5 0 29 129 178 628.5 0 178 130 365 628.5 0 365 131 161 628.5 0 161 132 70 628.5 0 70 133 35 628.5 0 35 134 44 628.5 0 44 135 208 628.5 0 208 136 225 628.5 0 225 137 156 628.5 0 156 138 75 628.5 0 75 139 67 628.5 0 67 140 43 628.5 0 43 141 72 628.5 0 72 142 542 628.5 0 542 143 1215 628.5 0 1215 144 1429 628.5 0 1429 145 597 628.5 0 597 146 235 628.5 0 235 147 407 628.5 0 407 148 22 628.5 0 22 149 22 628.5 0 22 150 19 628.5 0 19 151 16 628.5 0 16 152 22 628.5 0 22 153 14 628.5 0 14 154 10 628.5 0 10 155 18 628.5 0 18 156 33 628.5 0 33 157 30 628.5 0 30 158 47 628.5 0 47 159 34 628.5 0 34 160 54 628.5 0 54 161 47 628.5 0 47 162 38 628.5 0 38 163 19 628.5 0 19 164 19 628.5 0 19 165 16 628.5 0 16 166 32 628.5 0 32 167 25 628.5 0 25 168 47 628.5 0 47 169 15 628.5 0 15 170 16 628.5 0 16 171 10 628.5 0 10 172 10 628.5 0 10 173 10 628.5 0 10 174 14 628.5 0 14 175 30 628.5 0 30 176 20 628.5 0 20 177 32 628.5 0 32 178 32 628.5 0 32 179 23 628.5 0 23 180 27 628.5 0 27 181 19 628.5 0 19 182 26 628.5 0 26 183 22 628.5 0 22 184 19 628.5 0 19 185 13 628.5 0 13 186 24 628.5 0 24 187 15 628.5 0 15 188 13 628.5 0 13 189 11 628.5 0 11 190 43 628.5 0 43 191 21 628.5 0 21 192 6 628.5 0 6 193 15 628.5 0 15 194 19 628.5 0 19 195 19 628.5 0 19 196 20 628.5 0 20 197 16 628.5 0 16 198 17 628.5 0 17 199 11 628.5 0 11 200 12 628.5 0 12 201 16 628.5 0 16 202 25 628.5 0 25 203 28 628.5 0 28 204 22 628.5 0 22 205 26 628.5 0 26 206 21 628.5 0 21 207 24 628.5 0 24 208 28 628.5 0 28 209 29 628.5 0 29 210 19 628.5 0 19 211 28 628.5 0 28 212 15 628.5 0 15 213 25 628.5 0 25 214 26 628.5 0 26 215 20 628.5 0 20 216 35 628.5 0 35 217 40 628.5 0 40 218 28 628.5 0 28 219 27 628.5 0 27 220 27 628.5 0 27 221 32 628.5 0 32 222 52 628.5 0 52 223 75 628.5 0 75 224 121 628.5 0 121 225 57 628.5 0 57 226 85 628.5 0 85 227 26 628.5 0 26 228 25 628.5 0 25 229 28 628.5 0 28 230 27 628.5 0 27 231 22 628.5 0 22 232 28 628.5 0 28 233 62 628.5 0 62 234 49 628.5 0 49 235 32 628.5 0 32 236 39 628.5 0 39 237 31 628.5 0 31 238 47 628.5 0 47 239 36 628.5 0 36 240 39 628.5 0 39 241 93 628.5 0 93 242 97 628.5 0 97 243 129 628.5 0 129 244 72 628.5 0 72 245 45 628.5 0 45 246 24 628.5 0 24 247 30 628.5 0 30 248 25 628.5 0 25 249 17 628.5 0 17 250 23 628.5 0 23 251 36 628.5 0 36 252 52 628.5 0 52 253 39 628.5 0 39 254 56 628.5 0 56 255 60 628.5 0 60 256 83 628.5 0 83 257 91 628.5 0 91 258 73 628.5 0 73 259 70 628.5 0 70 260 46 628.5 0 46 261 50 628.5 0 50 262 149 628.5 0 149 263 217 628.5 0 217 264 242 628.5 0 242 265 151 628.5 0 151 266 32 628.5 0 32 267 33 628.5 0 33 268 37 628.5 0 37 269 33 628.5 0 33 270 51 628.5 0 51 271 50 628.5 0 50 272 50 628.5 0 50 273 57 628.5 0 57 274 39 628.5 0 39 275 79 628.5 0 79 276 102 628.5 0 102 277 224 628.5 0 224 278 174 628.5 0 174 279 67 628.5 0 67 280 78 628.5 0 78 281 76 628.5 0 76 282 583 628.5 0 583 283 194 628.5 0 194 284 393 628.5 0 393 285 124 628.5 0 124 286 53 628.5 0 53 287 46 628.5 0 46 288 48 628.5 0 48 289 44 628.5 0 44 290 118 628.5 0 118 291 190 628.5 0 190 292 269 628.5 0 269 293 123 628.5 0 123 294 631 628.5 0 631 295 831 628.5 0 831 296 332 628.5 0 332 297 57 628.5 0 57 298 61 628.5 0 61 299 66 628.5 0 66 300 60 628.5 0 60 301 33 628.5 0 33 Finished in 2.25 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 45,920 Reads with adapters: 13,632 (29.7%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 13,584 (29.6%) Total basepairs processed: 11,359,568 bp Total written (filtered): 3,699,714 bp (32.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13632 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 2 0.0 3 0 0 2 19 171 0.0 3 0 136 18 10 7 20 13097 0.0 4 11995 870 161 48 23 21 43 0.0 4 15 21 6 1 22 19 0.0 4 14 2 2 0 1 23 42 0.0 4 4 0 0 28 10 25 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 35 49 0.0 4 0 0 0 0 49 37 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 0 2 39 1 0.0 4 1 53 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 1 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 0 2 100 2 0.0 4 0 0 0 0 2 101 4 0.0 4 0 0 0 0 4 109 1 0.0 4 0 0 0 0 1 122 5 0.0 4 0 0 0 0 5 123 3 0.0 4 0 0 0 0 3 124 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 133 0.0 4 0 0 0 0 133 128 4 0.0 4 0 0 0 1 3 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 171 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 1 207 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 233 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 13,584 Reads with adapters: 712 (5.2%) Reads written (passing filters): 13,584 (100.0%) Total basepairs processed: 3,699,714 bp Total written (filtered): 3,691,878 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 712 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.6% C: 18.3% G: 66.4% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 97 212.2 0 97 4 18 53.1 0 18 5 1 13.3 0 1 9 1 0.1 0 0 1 10 12 0.0 1 1 11 11 357 0.0 1 12 345 12 174 0.0 1 8 166 13 14 0.0 1 0 14 15 1 0.0 1 0 1 16 1 0.0 1 0 1 20 1 0.0 1 1 24 32 0.0 1 24 8 25 2 0.0 1 1 1 273 1 0.0 1 0 1