This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.13 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 52,736 (2.0%) Reads that were too short: 7,206 (0.3%) Reads written (passing filters): 45,530 (1.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,484,371 bp (1.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 52736 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 809 628.5 0 809 7 15550 628.5 0 15550 8 398 628.5 0 398 9 12320 628.5 0 12320 10 9 628.5 0 9 11 1 628.5 0 1 12 1 628.5 0 1 13 2 628.5 0 2 15 2 628.5 0 2 17 2 628.5 0 2 18 2 628.5 0 2 20 1 628.5 0 1 22 2 628.5 0 2 24 2 628.5 0 2 25 1 628.5 0 1 28 1 628.5 0 1 29 1 628.5 0 1 30 7 628.5 0 7 31 2 628.5 0 2 32 1 628.5 0 1 33 4 628.5 0 4 35 4 628.5 0 4 37 3 628.5 0 3 38 2 628.5 0 2 39 10 628.5 0 10 40 10 628.5 0 10 41 774 628.5 0 774 42 74 628.5 0 74 43 5 628.5 0 5 46 4 628.5 0 4 47 3 628.5 0 3 48 7 628.5 0 7 49 4 628.5 0 4 50 2 628.5 0 2 51 1 628.5 0 1 52 5 628.5 0 5 53 2 628.5 0 2 54 6 628.5 0 6 56 2 628.5 0 2 57 2 628.5 0 2 58 3 628.5 0 3 59 4 628.5 0 4 60 7 628.5 0 7 61 4 628.5 0 4 62 2 628.5 0 2 64 2 628.5 0 2 65 4 628.5 0 4 66 7 628.5 0 7 67 5 628.5 0 5 68 5 628.5 0 5 70 4 628.5 0 4 71 6 628.5 0 6 72 2 628.5 0 2 73 4 628.5 0 4 74 5 628.5 0 5 75 5 628.5 0 5 76 7 628.5 0 7 77 14 628.5 0 14 78 42 628.5 0 42 79 20 628.5 0 20 80 8 628.5 0 8 81 13 628.5 0 13 82 16 628.5 0 16 83 11 628.5 0 11 84 8 628.5 0 8 85 6 628.5 0 6 86 18 628.5 0 18 87 10 628.5 0 10 88 11 628.5 0 11 89 13 628.5 0 13 90 12 628.5 0 12 91 12 628.5 0 12 92 15 628.5 0 15 93 9 628.5 0 9 94 14 628.5 0 14 95 25 628.5 0 25 96 32 628.5 0 32 97 34 628.5 0 34 98 61 628.5 0 61 99 246 628.5 0 246 100 310 628.5 0 310 101 294 628.5 0 294 102 37 628.5 0 37 103 20 628.5 0 20 104 26 628.5 0 26 105 23 628.5 0 23 106 30 628.5 0 30 107 49 628.5 0 49 108 43 628.5 0 43 109 77 628.5 0 77 110 61 628.5 0 61 111 888 628.5 0 888 112 1611 628.5 0 1611 113 2259 628.5 0 2259 114 514 628.5 0 514 115 1272 628.5 0 1272 116 15 628.5 0 15 117 10 628.5 0 10 118 8 628.5 0 8 119 30 628.5 0 30 120 14 628.5 0 14 121 6 628.5 0 6 122 14 628.5 0 14 123 12 628.5 0 12 124 10 628.5 0 10 125 20 628.5 0 20 126 18 628.5 0 18 127 17 628.5 0 17 128 21 628.5 0 21 129 118 628.5 0 118 130 271 628.5 0 271 131 112 628.5 0 112 132 36 628.5 0 36 133 30 628.5 0 30 134 25 628.5 0 25 135 219 628.5 0 219 136 211 628.5 0 211 137 93 628.5 0 93 138 37 628.5 0 37 139 56 628.5 0 56 140 50 628.5 0 50 141 50 628.5 0 50 142 701 628.5 0 701 143 1499 628.5 0 1499 144 1898 628.5 0 1898 145 645 628.5 0 645 146 82 628.5 0 82 147 137 628.5 0 137 148 7 628.5 0 7 149 13 628.5 0 13 150 15 628.5 0 15 151 24 628.5 0 24 152 14 628.5 0 14 153 11 628.5 0 11 154 8 628.5 0 8 155 10 628.5 0 10 156 11 628.5 0 11 157 16 628.5 0 16 158 16 628.5 0 16 159 25 628.5 0 25 160 29 628.5 0 29 161 35 628.5 0 35 162 22 628.5 0 22 163 14 628.5 0 14 164 9 628.5 0 9 165 9 628.5 0 9 166 15 628.5 0 15 167 17 628.5 0 17 168 18 628.5 0 18 169 10 628.5 0 10 170 13 628.5 0 13 171 10 628.5 0 10 172 10 628.5 0 10 173 10 628.5 0 10 174 9 628.5 0 9 175 15 628.5 0 15 176 10 628.5 0 10 177 24 628.5 0 24 178 12 628.5 0 12 179 19 628.5 0 19 180 11 628.5 0 11 181 5 628.5 0 5 182 12 628.5 0 12 183 5 628.5 0 5 184 9 628.5 0 9 185 10 628.5 0 10 186 14 628.5 0 14 187 13 628.5 0 13 188 16 628.5 0 16 189 20 628.5 0 20 190 54 628.5 0 54 191 6 628.5 0 6 192 8 628.5 0 8 193 19 628.5 0 19 194 9 628.5 0 9 195 10 628.5 0 10 196 15 628.5 0 15 197 19 628.5 0 19 198 7 628.5 0 7 199 16 628.5 0 16 200 13 628.5 0 13 201 16 628.5 0 16 202 24 628.5 0 24 203 16 628.5 0 16 204 17 628.5 0 17 205 13 628.5 0 13 206 22 628.5 0 22 207 21 628.5 0 21 208 21 628.5 0 21 209 21 628.5 0 21 210 19 628.5 0 19 211 16 628.5 0 16 212 15 628.5 0 15 213 17 628.5 0 17 214 14 628.5 0 14 215 17 628.5 0 17 216 9 628.5 0 9 217 18 628.5 0 18 218 26 628.5 0 26 219 21 628.5 0 21 220 24 628.5 0 24 221 34 628.5 0 34 222 28 628.5 0 28 223 37 628.5 0 37 224 146 628.5 0 146 225 45 628.5 0 45 226 97 628.5 0 97 227 16 628.5 0 16 228 12 628.5 0 12 229 24 628.5 0 24 230 21 628.5 0 21 231 16 628.5 0 16 232 23 628.5 0 23 233 32 628.5 0 32 234 20 628.5 0 20 235 21 628.5 0 21 236 26 628.5 0 26 237 25 628.5 0 25 238 19 628.5 0 19 239 31 628.5 0 31 240 24 628.5 0 24 241 36 628.5 0 36 242 45 628.5 0 45 243 51 628.5 0 51 244 36 628.5 0 36 245 25 628.5 0 25 246 18 628.5 0 18 247 22 628.5 0 22 248 11 628.5 0 11 249 26 628.5 0 26 250 22 628.5 0 22 251 34 628.5 0 34 252 31 628.5 0 31 253 24 628.5 0 24 254 31 628.5 0 31 255 29 628.5 0 29 256 34 628.5 0 34 257 44 628.5 0 44 258 37 628.5 0 37 259 39 628.5 0 39 260 61 628.5 0 61 261 27 628.5 0 27 262 58 628.5 0 58 263 71 628.5 0 71 264 77 628.5 0 77 265 38 628.5 0 38 266 19 628.5 0 19 267 18 628.5 0 18 268 22 628.5 0 22 269 22 628.5 0 22 270 36 628.5 0 36 271 34 628.5 0 34 272 26 628.5 0 26 273 38 628.5 0 38 274 26 628.5 0 26 275 30 628.5 0 30 276 88 628.5 0 88 277 160 628.5 0 160 278 83 628.5 0 83 279 43 628.5 0 43 280 36 628.5 0 36 281 53 628.5 0 53 282 345 628.5 0 345 283 116 628.5 0 116 284 184 628.5 0 184 285 62 628.5 0 62 286 35 628.5 0 35 287 26 628.5 0 26 288 40 628.5 0 40 289 49 628.5 0 49 290 127 628.5 0 127 291 167 628.5 0 167 292 377 628.5 0 377 293 134 628.5 0 134 294 886 628.5 0 886 295 1269 628.5 0 1269 296 485 628.5 0 485 297 50 628.5 0 50 298 38 628.5 0 38 299 46 628.5 0 46 300 34 628.5 0 34 301 27 628.5 0 27 Finished in 2.05 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 45,530 Reads with adapters: 12,950 (28.4%) Reads that were too short: 52 (0.1%) Reads written (passing filters): 12,898 (28.3%) Total basepairs processed: 11,484,371 bp Total written (filtered): 3,539,308 bp (30.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12950 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 103 0.0 3 0 101 2 17 12688 0.0 3 12449 226 13 18 29 0.0 3 6 20 3 19 9 0.0 3 2 0 5 2 20 6 0.0 3 1 0 0 5 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 29 28 0.0 3 0 0 0 28 32 6 0.0 3 0 0 0 6 37 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 62 6 0.0 3 0 0 0 6 65 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 144 8 0.0 3 0 0 0 8 145 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 31 0.0 3 0 0 0 31 221 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.46 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 12,898 Reads with adapters: 229 (1.8%) Reads written (passing filters): 12,898 (100.0%) Total basepairs processed: 3,539,308 bp Total written (filtered): 3,538,613 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 229 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.0% C: 13.1% G: 30.1% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 221 201.5 0 221 4 8 50.4 0 8