This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.88 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 131,372 (5.1%) Reads that were too short: 15,379 (0.6%) Reads written (passing filters): 115,993 (4.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 31,090,253 bp (4.0%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 131372 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1225 628.5 0 1225 7 48486 628.5 0 48486 8 1872 628.5 0 1872 9 37852 628.5 0 37852 10 91 628.5 0 91 11 55 628.5 0 55 12 84 628.5 0 84 13 96 628.5 0 96 14 77 628.5 0 77 15 54 628.5 0 54 16 73 628.5 0 73 17 72 628.5 0 72 18 57 628.5 0 57 19 41 628.5 0 41 20 48 628.5 0 48 21 38 628.5 0 38 22 48 628.5 0 48 23 36 628.5 0 36 24 45 628.5 0 45 25 51 628.5 0 51 26 49 628.5 0 49 27 65 628.5 0 65 28 91 628.5 0 91 29 80 628.5 0 80 30 79 628.5 0 79 31 71 628.5 0 71 32 59 628.5 0 59 33 73 628.5 0 73 34 55 628.5 0 55 35 55 628.5 0 55 36 68 628.5 0 68 37 73 628.5 0 73 38 68 628.5 0 68 39 55 628.5 0 55 40 91 628.5 0 91 41 74 628.5 0 74 42 73 628.5 0 73 43 73 628.5 0 73 44 266 628.5 0 266 45 157 628.5 0 157 46 286 628.5 0 286 47 49 628.5 0 49 48 35 628.5 0 35 49 50 628.5 0 50 50 46 628.5 0 46 51 83 628.5 0 83 52 84 628.5 0 84 53 108 628.5 0 108 54 48 628.5 0 48 55 46 628.5 0 46 56 83 628.5 0 83 57 112 628.5 0 112 58 503 628.5 0 503 59 415 628.5 0 415 60 336 628.5 0 336 61 60 628.5 0 60 62 51 628.5 0 51 63 43 628.5 0 43 64 36 628.5 0 36 65 36 628.5 0 36 66 53 628.5 0 53 67 57 628.5 0 57 68 64 628.5 0 64 69 46 628.5 0 46 70 40 628.5 0 40 71 49 628.5 0 49 72 49 628.5 0 49 73 71 628.5 0 71 74 53 628.5 0 53 75 92 628.5 0 92 76 69 628.5 0 69 77 82 628.5 0 82 78 51 628.5 0 51 79 44 628.5 0 44 80 64 628.5 0 64 81 47 628.5 0 47 82 67 628.5 0 67 83 77 628.5 0 77 84 66 628.5 0 66 85 57 628.5 0 57 86 60 628.5 0 60 87 51 628.5 0 51 88 68 628.5 0 68 89 60 628.5 0 60 90 70 628.5 0 70 91 114 628.5 0 114 92 129 628.5 0 129 93 187 628.5 0 187 94 88 628.5 0 88 95 66 628.5 0 66 96 62 628.5 0 62 97 110 628.5 0 110 98 67 628.5 0 67 99 80 628.5 0 80 100 60 628.5 0 60 101 87 628.5 0 87 102 80 628.5 0 80 103 63 628.5 0 63 104 84 628.5 0 84 105 119 628.5 0 119 106 713 628.5 0 713 107 423 628.5 0 423 108 613 628.5 0 613 109 94 628.5 0 94 110 79 628.5 0 79 111 78 628.5 0 78 112 90 628.5 0 90 113 75 628.5 0 75 114 526 628.5 0 526 115 660 628.5 0 660 116 546 628.5 0 546 117 87 628.5 0 87 118 59 628.5 0 59 119 63 628.5 0 63 120 44 628.5 0 44 121 64 628.5 0 64 122 72 628.5 0 72 123 84 628.5 0 84 124 109 628.5 0 109 125 85 628.5 0 85 126 69 628.5 0 69 127 88 628.5 0 88 128 386 628.5 0 386 129 270 628.5 0 270 130 239 628.5 0 239 131 92 628.5 0 92 132 97 628.5 0 97 133 79 628.5 0 79 134 194 628.5 0 194 135 171 628.5 0 171 136 423 628.5 0 423 137 338 628.5 0 338 138 246 628.5 0 246 139 167 628.5 0 167 140 439 628.5 0 439 141 427 628.5 0 427 142 595 628.5 0 595 143 939 628.5 0 939 144 103 628.5 0 103 145 119 628.5 0 119 146 74 628.5 0 74 147 83 628.5 0 83 148 1067 628.5 0 1067 149 731 628.5 0 731 150 848 628.5 0 848 151 64 628.5 0 64 152 46 628.5 0 46 153 67 628.5 0 67 154 93 628.5 0 93 155 431 628.5 0 431 156 243 628.5 0 243 157 381 628.5 0 381 158 121 628.5 0 121 159 53 628.5 0 53 160 62 628.5 0 62 161 107 628.5 0 107 162 105 628.5 0 105 163 374 628.5 0 374 164 342 628.5 0 342 165 499 628.5 0 499 166 83 628.5 0 83 167 46 628.5 0 46 168 89 628.5 0 89 169 57 628.5 0 57 170 97 628.5 0 97 171 18 628.5 0 18 172 29 628.5 0 29 173 42 628.5 0 42 174 45 628.5 0 45 175 47 628.5 0 47 176 49 628.5 0 49 177 45 628.5 0 45 178 56 628.5 0 56 179 85 628.5 0 85 180 309 628.5 0 309 181 156 628.5 0 156 182 190 628.5 0 190 183 85 628.5 0 85 184 68 628.5 0 68 185 95 628.5 0 95 186 96 628.5 0 96 187 42 628.5 0 42 188 69 628.5 0 69 189 54 628.5 0 54 190 57 628.5 0 57 191 76 628.5 0 76 192 50 628.5 0 50 193 79 628.5 0 79 194 45 628.5 0 45 195 58 628.5 0 58 196 63 628.5 0 63 197 56 628.5 0 56 198 59 628.5 0 59 199 53 628.5 0 53 200 53 628.5 0 53 201 67 628.5 0 67 202 339 628.5 0 339 203 169 628.5 0 169 204 156 628.5 0 156 205 44 628.5 0 44 206 57 628.5 0 57 207 42 628.5 0 42 208 68 628.5 0 68 209 78 628.5 0 78 210 54 628.5 0 54 211 63 628.5 0 63 212 52 628.5 0 52 213 101 628.5 0 101 214 122 628.5 0 122 215 125 628.5 0 125 216 119 628.5 0 119 217 77 628.5 0 77 218 61 628.5 0 61 219 50 628.5 0 50 220 54 628.5 0 54 221 50 628.5 0 50 222 67 628.5 0 67 223 88 628.5 0 88 224 73 628.5 0 73 225 62 628.5 0 62 226 85 628.5 0 85 227 56 628.5 0 56 228 77 628.5 0 77 229 52 628.5 0 52 230 56 628.5 0 56 231 71 628.5 0 71 232 94 628.5 0 94 233 153 628.5 0 153 234 200 628.5 0 200 235 138 628.5 0 138 236 95 628.5 0 95 237 51 628.5 0 51 238 54 628.5 0 54 239 71 628.5 0 71 240 58 628.5 0 58 241 63 628.5 0 63 242 95 628.5 0 95 243 90 628.5 0 90 244 121 628.5 0 121 245 77 628.5 0 77 246 58 628.5 0 58 247 70 628.5 0 70 248 75 628.5 0 75 249 78 628.5 0 78 250 63 628.5 0 63 251 92 628.5 0 92 252 102 628.5 0 102 253 145 628.5 0 145 254 87 628.5 0 87 255 172 628.5 0 172 256 58 628.5 0 58 257 74 628.5 0 74 258 85 628.5 0 85 259 66 628.5 0 66 260 87 628.5 0 87 261 214 628.5 0 214 262 147 628.5 0 147 263 141 628.5 0 141 264 101 628.5 0 101 265 94 628.5 0 94 266 82 628.5 0 82 267 93 628.5 0 93 268 85 628.5 0 85 269 123 628.5 0 123 270 133 628.5 0 133 271 497 628.5 0 497 272 402 628.5 0 402 273 259 628.5 0 259 274 97 628.5 0 97 275 92 628.5 0 92 276 83 628.5 0 83 277 84 628.5 0 84 278 136 628.5 0 136 279 189 628.5 0 189 280 147 628.5 0 147 281 94 628.5 0 94 282 80 628.5 0 80 283 132 628.5 0 132 284 135 628.5 0 135 285 140 628.5 0 140 286 82 628.5 0 82 287 77 628.5 0 77 288 87 628.5 0 87 289 64 628.5 0 64 290 117 628.5 0 117 291 132 628.5 0 132 292 213 628.5 0 213 293 186 628.5 0 186 294 463 628.5 0 463 295 1786 628.5 0 1786 296 1329 628.5 0 1329 297 1318 628.5 0 1318 298 320 628.5 0 320 299 66 628.5 0 66 300 58 628.5 0 58 301 61 628.5 0 61 Finished in 5.99 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 115,993 Reads with adapters: 40,417 (34.8%) Reads that were too short: 70 (0.1%) Reads written (passing filters): 40,347 (34.8%) Total basepairs processed: 31,090,253 bp Total written (filtered): 10,950,493 bp (35.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 40417 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 4 5 18 34 0.0 3 0 0 8 15 11 19 497 0.0 3 0 341 60 70 26 20 39147 0.0 4 35379 2820 672 219 57 21 112 0.0 4 29 65 12 3 3 22 30 0.0 4 12 4 10 2 2 23 252 0.0 4 0 0 0 150 102 24 5 0.0 4 0 0 0 0 5 25 2 0.0 4 0 0 0 1 1 27 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 1 35 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 1 1 77 2 0.0 4 2 78 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 111 1 0.0 4 1 113 1 0.0 4 0 0 0 0 1 119 1 0.0 4 1 120 1 0.0 4 0 0 0 1 121 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 222 0.0 4 0 0 0 0 222 128 4 0.0 4 1 0 0 1 2 129 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 1 134 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 163 3 0.0 4 0 0 0 0 3 172 1 0.0 4 1 179 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 1 212 1 0.0 4 0 0 1 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 219 2 0.0 4 0 0 0 0 2 221 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 1 225 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 7 0.0 4 0 0 0 0 7 230 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 1 233 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 237 10 0.0 4 0 0 0 1 9 238 1 0.0 4 0 0 1 239 1 0.0 4 0 0 0 0 1 240 2 0.0 4 1 1 245 2 0.0 4 0 0 0 0 2 254 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 1 265 1 0.0 4 0 0 1 274 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.50 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 40,347 Reads with adapters: 9,867 (24.5%) Reads written (passing filters): 40,347 (100.0%) Total basepairs processed: 10,950,493 bp Total written (filtered): 10,781,858 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9867 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 9.7% G: 84.1% T: 3.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 30 630.4 0 30 4 22 157.6 0 22 6 1 9.9 0 1 7 4 2.5 0 4 8 4 0.6 0 4 9 1843 0.2 0 219 1624 10 5270 0.0 1 459 4811 11 2307 0.0 1 121 2186 12 33 0.0 1 1 32 13 27 0.0 1 0 27 14 4 0.0 1 0 4 18 4 0.0 1 4 19 14 0.0 1 11 3 20 1 0.0 1 1 22 7 0.0 1 6 1 70 1 0.0 1 1 81 1 0.0 1 0 1 89 1 0.0 1 1 90 2 0.0 1 2 105 1 0.0 1 0 1 106 1 0.0 1 1 107 2 0.0 1 1 1 115 1 0.0 1 1 147 1 0.0 1 0 1 150 6 0.0 1 5 1 151 2 0.0 1 1 1 171 1 0.0 1 1 177 2 0.0 1 1 1 204 1 0.0 1 1 207 1 0.0 1 1 208 6 0.0 1 5 1 215 57 0.0 1 51 6 216 2 0.0 1 2 238 1 0.0 1 1 241 2 0.0 1 2 267 184 0.0 1 174 10 268 10 0.0 1 10 269 2 0.0 1 1 1 270 2 0.0 1 2 271 6 0.0 1 5 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.