This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.15 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 118,791 (4.6%) Reads that were too short: 6,669 (0.3%) Reads written (passing filters): 112,122 (4.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 30,975,013 bp (4.0%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 118791 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 842 628.5 0 842 7 49484 628.5 0 49484 8 1860 628.5 0 1860 9 43239 628.5 0 43239 10 58 628.5 0 58 11 29 628.5 0 29 12 41 628.5 0 41 13 35 628.5 0 35 14 24 628.5 0 24 15 20 628.5 0 20 16 40 628.5 0 40 17 33 628.5 0 33 18 26 628.5 0 26 19 25 628.5 0 25 20 23 628.5 0 23 21 19 628.5 0 19 22 24 628.5 0 24 23 27 628.5 0 27 24 20 628.5 0 20 25 36 628.5 0 36 26 26 628.5 0 26 27 33 628.5 0 33 28 42 628.5 0 42 29 33 628.5 0 33 30 43 628.5 0 43 31 44 628.5 0 44 32 32 628.5 0 32 33 51 628.5 0 51 34 26 628.5 0 26 35 33 628.5 0 33 36 31 628.5 0 31 37 35 628.5 0 35 38 42 628.5 0 42 39 44 628.5 0 44 40 46 628.5 0 46 41 28 628.5 0 28 42 24 628.5 0 24 43 50 628.5 0 50 44 171 628.5 0 171 45 72 628.5 0 72 46 172 628.5 0 172 47 27 628.5 0 27 48 28 628.5 0 28 49 47 628.5 0 47 50 26 628.5 0 26 51 48 628.5 0 48 52 36 628.5 0 36 53 30 628.5 0 30 54 37 628.5 0 37 55 35 628.5 0 35 56 44 628.5 0 44 57 33 628.5 0 33 58 68 628.5 0 68 59 63 628.5 0 63 60 42 628.5 0 42 61 35 628.5 0 35 62 35 628.5 0 35 63 29 628.5 0 29 64 31 628.5 0 31 65 28 628.5 0 28 66 40 628.5 0 40 67 36 628.5 0 36 68 55 628.5 0 55 69 26 628.5 0 26 70 35 628.5 0 35 71 35 628.5 0 35 72 26 628.5 0 26 73 40 628.5 0 40 74 38 628.5 0 38 75 56 628.5 0 56 76 33 628.5 0 33 77 45 628.5 0 45 78 33 628.5 0 33 79 40 628.5 0 40 80 26 628.5 0 26 81 42 628.5 0 42 82 41 628.5 0 41 83 56 628.5 0 56 84 50 628.5 0 50 85 48 628.5 0 48 86 39 628.5 0 39 87 33 628.5 0 33 88 30 628.5 0 30 89 41 628.5 0 41 90 51 628.5 0 51 91 76 628.5 0 76 92 72 628.5 0 72 93 55 628.5 0 55 94 50 628.5 0 50 95 33 628.5 0 33 96 42 628.5 0 42 97 50 628.5 0 50 98 54 628.5 0 54 99 35 628.5 0 35 100 54 628.5 0 54 101 61 628.5 0 61 102 45 628.5 0 45 103 68 628.5 0 68 104 64 628.5 0 64 105 68 628.5 0 68 106 465 628.5 0 465 107 279 628.5 0 279 108 312 628.5 0 312 109 67 628.5 0 67 110 54 628.5 0 54 111 65 628.5 0 65 112 58 628.5 0 58 113 59 628.5 0 59 114 600 628.5 0 600 115 832 628.5 0 832 116 730 628.5 0 730 117 54 628.5 0 54 118 42 628.5 0 42 119 42 628.5 0 42 120 39 628.5 0 39 121 54 628.5 0 54 122 44 628.5 0 44 123 65 628.5 0 65 124 65 628.5 0 65 125 48 628.5 0 48 126 57 628.5 0 57 127 40 628.5 0 40 128 119 628.5 0 119 129 78 628.5 0 78 130 99 628.5 0 99 131 56 628.5 0 56 132 62 628.5 0 62 133 69 628.5 0 69 134 109 628.5 0 109 135 106 628.5 0 106 136 241 628.5 0 241 137 333 628.5 0 333 138 207 628.5 0 207 139 105 628.5 0 105 140 390 628.5 0 390 141 299 628.5 0 299 142 245 628.5 0 245 143 301 628.5 0 301 144 47 628.5 0 47 145 51 628.5 0 51 146 55 628.5 0 55 147 51 628.5 0 51 148 1047 628.5 0 1047 149 580 628.5 0 580 150 771 628.5 0 771 151 36 628.5 0 36 152 35 628.5 0 35 153 44 628.5 0 44 154 32 628.5 0 32 155 166 628.5 0 166 156 151 628.5 0 151 157 156 628.5 0 156 158 87 628.5 0 87 159 45 628.5 0 45 160 51 628.5 0 51 161 82 628.5 0 82 162 68 628.5 0 68 163 207 628.5 0 207 164 157 628.5 0 157 165 246 628.5 0 246 166 63 628.5 0 63 167 39 628.5 0 39 168 41 628.5 0 41 169 43 628.5 0 43 170 39 628.5 0 39 171 30 628.5 0 30 172 26 628.5 0 26 173 28 628.5 0 28 174 27 628.5 0 27 175 38 628.5 0 38 176 24 628.5 0 24 177 27 628.5 0 27 178 30 628.5 0 30 179 57 628.5 0 57 180 104 628.5 0 104 181 85 628.5 0 85 182 86 628.5 0 86 183 47 628.5 0 47 184 30 628.5 0 30 185 43 628.5 0 43 186 44 628.5 0 44 187 29 628.5 0 29 188 46 628.5 0 46 189 44 628.5 0 44 190 28 628.5 0 28 191 37 628.5 0 37 192 51 628.5 0 51 193 49 628.5 0 49 194 30 628.5 0 30 195 34 628.5 0 34 196 33 628.5 0 33 197 30 628.5 0 30 198 44 628.5 0 44 199 34 628.5 0 34 200 48 628.5 0 48 201 52 628.5 0 52 202 176 628.5 0 176 203 93 628.5 0 93 204 83 628.5 0 83 205 33 628.5 0 33 206 33 628.5 0 33 207 27 628.5 0 27 208 34 628.5 0 34 209 60 628.5 0 60 210 41 628.5 0 41 211 27 628.5 0 27 212 42 628.5 0 42 213 64 628.5 0 64 214 46 628.5 0 46 215 54 628.5 0 54 216 67 628.5 0 67 217 47 628.5 0 47 218 43 628.5 0 43 219 26 628.5 0 26 220 28 628.5 0 28 221 38 628.5 0 38 222 47 628.5 0 47 223 64 628.5 0 64 224 46 628.5 0 46 225 38 628.5 0 38 226 45 628.5 0 45 227 38 628.5 0 38 228 39 628.5 0 39 229 44 628.5 0 44 230 43 628.5 0 43 231 34 628.5 0 34 232 54 628.5 0 54 233 65 628.5 0 65 234 55 628.5 0 55 235 65 628.5 0 65 236 58 628.5 0 58 237 49 628.5 0 49 238 48 628.5 0 48 239 51 628.5 0 51 240 29 628.5 0 29 241 50 628.5 0 50 242 42 628.5 0 42 243 41 628.5 0 41 244 63 628.5 0 63 245 45 628.5 0 45 246 33 628.5 0 33 247 48 628.5 0 48 248 66 628.5 0 66 249 67 628.5 0 67 250 45 628.5 0 45 251 33 628.5 0 33 252 84 628.5 0 84 253 65 628.5 0 65 254 56 628.5 0 56 255 58 628.5 0 58 256 51 628.5 0 51 257 56 628.5 0 56 258 45 628.5 0 45 259 35 628.5 0 35 260 51 628.5 0 51 261 72 628.5 0 72 262 79 628.5 0 79 263 71 628.5 0 71 264 40 628.5 0 40 265 56 628.5 0 56 266 59 628.5 0 59 267 42 628.5 0 42 268 59 628.5 0 59 269 47 628.5 0 47 270 67 628.5 0 67 271 165 628.5 0 165 272 171 628.5 0 171 273 132 628.5 0 132 274 79 628.5 0 79 275 87 628.5 0 87 276 56 628.5 0 56 277 49 628.5 0 49 278 78 628.5 0 78 279 78 628.5 0 78 280 89 628.5 0 89 281 69 628.5 0 69 282 44 628.5 0 44 283 53 628.5 0 53 284 72 628.5 0 72 285 57 628.5 0 57 286 49 628.5 0 49 287 34 628.5 0 34 288 68 628.5 0 68 289 53 628.5 0 53 290 57 628.5 0 57 291 57 628.5 0 57 292 89 628.5 0 89 293 78 628.5 0 78 294 125 628.5 0 125 295 329 628.5 0 329 296 273 628.5 0 273 297 325 628.5 0 325 298 105 628.5 0 105 299 49 628.5 0 49 300 43 628.5 0 43 301 56 628.5 0 56 Finished in 5.29 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 112,122 Reads with adapters: 49,648 (44.3%) Reads that were too short: 85 (0.1%) Reads written (passing filters): 49,563 (44.2%) Total basepairs processed: 30,975,013 bp Total written (filtered): 13,718,546 bp (44.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49648 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 13 0.0 3 0 0 13 16 251 0.0 3 0 248 3 17 49045 0.0 3 48054 958 29 4 18 112 0.0 3 7 101 3 1 19 29 0.0 3 3 0 24 2 20 43 0.0 3 0 0 0 43 22 2 0.0 3 2 27 1 0.0 3 0 0 0 1 30 3 0.0 3 0 0 0 3 33 1 0.0 3 1 38 1 0.0 3 0 0 1 43 17 0.0 3 0 0 0 17 44 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 69 15 0.0 3 0 0 0 15 70 10 0.0 3 0 0 0 10 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 75 1 0.0 3 1 80 1 0.0 3 0 1 103 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 124 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 1 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 59 0.0 3 0 0 3 56 221 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.74 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 49,563 Reads with adapters: 180 (0.4%) Reads written (passing filters): 49,563 (100.0%) Total basepairs processed: 13,718,546 bp Total written (filtered): 13,717,977 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 180 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.2% C: 35.0% G: 20.0% T: 7.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 158 774.4 0 158 4 17 193.6 0 17 5 4 48.4 0 4 7 1 3.0 0 1