This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.40 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 100,445 (3.9%) Reads that were too short: 52,064 (2.0%) Reads written (passing filters): 48,381 (1.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,166,748 bp (1.4%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 100445 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1088 628.5 0 1088 7 11097 628.5 0 11097 8 1039 628.5 0 1039 9 12271 628.5 0 12271 10 234 628.5 0 234 11 36 628.5 0 36 12 33 628.5 0 33 13 33 628.5 0 33 14 47 628.5 0 47 15 33 628.5 0 33 16 92 628.5 0 92 17 73 628.5 0 73 18 228 628.5 0 228 19 33 628.5 0 33 20 38 628.5 0 38 21 46 628.5 0 46 22 35 628.5 0 35 23 24 628.5 0 24 24 28 628.5 0 28 25 74 628.5 0 74 26 36 628.5 0 36 27 39 628.5 0 39 28 38 628.5 0 38 29 32 628.5 0 32 30 40 628.5 0 40 31 38 628.5 0 38 32 37 628.5 0 37 33 30 628.5 0 30 34 38 628.5 0 38 35 43 628.5 0 43 36 28 628.5 0 28 37 39 628.5 0 39 38 29 628.5 0 29 39 23 628.5 0 23 40 25 628.5 0 25 41 33 628.5 0 33 42 33 628.5 0 33 43 32 628.5 0 32 44 30 628.5 0 30 45 34 628.5 0 34 46 27 628.5 0 27 47 26 628.5 0 26 48 52 628.5 0 52 49 42 628.5 0 42 50 121 628.5 0 121 51 72 628.5 0 72 52 36 628.5 0 36 53 46 628.5 0 46 54 46 628.5 0 46 55 27 628.5 0 27 56 41 628.5 0 41 57 32 628.5 0 32 58 28 628.5 0 28 59 37 628.5 0 37 60 43 628.5 0 43 61 63 628.5 0 63 62 42 628.5 0 42 63 53 628.5 0 53 64 35 628.5 0 35 65 39 628.5 0 39 66 56 628.5 0 56 67 446 628.5 0 446 68 479 628.5 0 479 69 377 628.5 0 377 70 26 628.5 0 26 71 65 628.5 0 65 72 55 628.5 0 55 73 45 628.5 0 45 74 54 628.5 0 54 75 36 628.5 0 36 76 38 628.5 0 38 77 40 628.5 0 40 78 33 628.5 0 33 79 28 628.5 0 28 80 36 628.5 0 36 81 40 628.5 0 40 82 37 628.5 0 37 83 44 628.5 0 44 84 41 628.5 0 41 85 87 628.5 0 87 86 178 628.5 0 178 87 68 628.5 0 68 88 49 628.5 0 49 89 47 628.5 0 47 90 63 628.5 0 63 91 27 628.5 0 27 92 47 628.5 0 47 93 30 628.5 0 30 94 52 628.5 0 52 95 40 628.5 0 40 96 56 628.5 0 56 97 59 628.5 0 59 98 58 628.5 0 58 99 34 628.5 0 34 100 38 628.5 0 38 101 27 628.5 0 27 102 24 628.5 0 24 103 37 628.5 0 37 104 31 628.5 0 31 105 24 628.5 0 24 106 48 628.5 0 48 107 32 628.5 0 32 108 28 628.5 0 28 109 30 628.5 0 30 110 45 628.5 0 45 111 38 628.5 0 38 112 33 628.5 0 33 113 56 628.5 0 56 114 35 628.5 0 35 115 36 628.5 0 36 116 46 628.5 0 46 117 60 628.5 0 60 118 110 628.5 0 110 119 109 628.5 0 109 120 118 628.5 0 118 121 113 628.5 0 113 122 94 628.5 0 94 123 70 628.5 0 70 124 39 628.5 0 39 125 37 628.5 0 37 126 78 628.5 0 78 127 87 628.5 0 87 128 143 628.5 0 143 129 105 628.5 0 105 130 62 628.5 0 62 131 141 628.5 0 141 132 110 628.5 0 110 133 126 628.5 0 126 134 100 628.5 0 100 135 52 628.5 0 52 136 51 628.5 0 51 137 32 628.5 0 32 138 51 628.5 0 51 139 50 628.5 0 50 140 48 628.5 0 48 141 34 628.5 0 34 142 43 628.5 0 43 143 35 628.5 0 35 144 49 628.5 0 49 145 72 628.5 0 72 146 84 628.5 0 84 147 108 628.5 0 108 148 91 628.5 0 91 149 81 628.5 0 81 150 81 628.5 0 81 151 103 628.5 0 103 152 516 628.5 0 516 153 574 628.5 0 574 154 798 628.5 0 798 155 999 628.5 0 999 156 52 628.5 0 52 157 54 628.5 0 54 158 144 628.5 0 144 159 392 628.5 0 392 160 191 628.5 0 191 161 87 628.5 0 87 162 81 628.5 0 81 163 356 628.5 0 356 164 561 628.5 0 561 165 716 628.5 0 716 166 287 628.5 0 287 167 141 628.5 0 141 168 64 628.5 0 64 169 73 628.5 0 73 170 44 628.5 0 44 171 52 628.5 0 52 172 73 628.5 0 73 173 87 628.5 0 87 174 119 628.5 0 119 175 126 628.5 0 126 176 66 628.5 0 66 177 116 628.5 0 116 178 141 628.5 0 141 179 191 628.5 0 191 180 265 628.5 0 265 181 152 628.5 0 152 182 130 628.5 0 130 183 616 628.5 0 616 184 879 628.5 0 879 185 651 628.5 0 651 186 492 628.5 0 492 187 197 628.5 0 197 188 61 628.5 0 61 189 84 628.5 0 84 190 82 628.5 0 82 191 121 628.5 0 121 192 412 628.5 0 412 193 595 628.5 0 595 194 446 628.5 0 446 195 367 628.5 0 367 196 65 628.5 0 65 197 73 628.5 0 73 198 221 628.5 0 221 199 332 628.5 0 332 200 349 628.5 0 349 201 277 628.5 0 277 202 194 628.5 0 194 203 125 628.5 0 125 204 103 628.5 0 103 205 170 628.5 0 170 206 218 628.5 0 218 207 230 628.5 0 230 208 181 628.5 0 181 209 174 628.5 0 174 210 113 628.5 0 113 211 85 628.5 0 85 212 145 628.5 0 145 213 116 628.5 0 116 214 210 628.5 0 210 215 265 628.5 0 265 216 285 628.5 0 285 217 582 628.5 0 582 218 923 628.5 0 923 219 9884 628.5 0 9884 220 6622 628.5 0 6622 221 15212 628.5 0 15212 222 366 628.5 0 366 223 112 628.5 0 112 224 114 628.5 0 114 225 47 628.5 0 47 226 69 628.5 0 69 227 48 628.5 0 48 228 84 628.5 0 84 229 89 628.5 0 89 230 118 628.5 0 118 231 114 628.5 0 114 232 96 628.5 0 96 233 85 628.5 0 85 234 134 628.5 0 134 235 179 628.5 0 179 236 120 628.5 0 120 237 175 628.5 0 175 238 143 628.5 0 143 239 193 628.5 0 193 240 231 628.5 0 231 241 168 628.5 0 168 242 95 628.5 0 95 243 61 628.5 0 61 244 82 628.5 0 82 245 64 628.5 0 64 246 83 628.5 0 83 247 112 628.5 0 112 248 344 628.5 0 344 249 621 628.5 0 621 250 588 628.5 0 588 251 277 628.5 0 277 252 213 628.5 0 213 253 265 628.5 0 265 254 352 628.5 0 352 255 141 628.5 0 141 256 103 628.5 0 103 257 98 628.5 0 98 258 110 628.5 0 110 259 122 628.5 0 122 260 107 628.5 0 107 261 93 628.5 0 93 262 99 628.5 0 99 263 90 628.5 0 90 264 98 628.5 0 98 265 77 628.5 0 77 266 101 628.5 0 101 267 158 628.5 0 158 268 154 628.5 0 154 269 150 628.5 0 150 270 117 628.5 0 117 271 93 628.5 0 93 272 215 628.5 0 215 273 325 628.5 0 325 274 251 628.5 0 251 275 179 628.5 0 179 276 88 628.5 0 88 277 137 628.5 0 137 278 259 628.5 0 259 279 859 628.5 0 859 280 1061 628.5 0 1061 281 1039 628.5 0 1039 282 310 628.5 0 310 283 146 628.5 0 146 284 206 628.5 0 206 285 134 628.5 0 134 286 132 628.5 0 132 287 173 628.5 0 173 288 191 628.5 0 191 289 300 628.5 0 300 290 131 628.5 0 131 291 116 628.5 0 116 292 257 628.5 0 257 293 250 628.5 0 250 294 308 628.5 0 308 295 170 628.5 0 170 296 157 628.5 0 157 297 146 628.5 0 146 298 150 628.5 0 150 299 215 628.5 0 215 300 280 628.5 0 280 301 289 628.5 0 289 Finished in 2.31 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 48,381 Reads with adapters: 12,450 (25.7%) Reads that were too short: 45 (0.1%) Reads written (passing filters): 12,405 (25.6%) Total basepairs processed: 11,166,748 bp Total written (filtered): 3,347,405 bp (30.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12450 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 4 0.0 3 0 0 2 2 19 204 0.0 3 0 170 18 13 3 20 11649 0.0 4 10774 692 131 44 8 21 24 0.0 4 2 18 1 3 22 1 0.0 4 0 0 1 23 33 0.0 4 0 0 0 21 12 25 1 0.0 4 1 27 2 0.0 4 1 0 0 0 1 28 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 50 2 0.0 4 1 0 0 0 1 58 1 0.0 4 0 0 1 63 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 88 2 0.0 4 0 0 0 1 1 89 2 0.0 4 0 0 0 0 2 105 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 469 0.0 4 0 0 0 0 469 128 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 202 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 1 222 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 7 0.0 4 0 0 0 0 7 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 12,405 Reads with adapters: 616 (5.0%) Reads written (passing filters): 12,405 (100.0%) Total basepairs processed: 3,347,405 bp Total written (filtered): 3,338,680 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 616 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.2% C: 14.0% G: 70.3% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 71 193.8 0 71 4 21 48.5 0 21 9 2 0.0 0 0 2 10 8 0.0 1 0 8 11 272 0.0 1 13 259 12 215 0.0 1 1 214 13 12 0.0 1 0 12 15 2 0.0 1 0 2 152 5 0.0 1 3 2 172 1 0.0 1 1 210 3 0.0 1 3 225 1 0.0 1 1 252 1 0.0 1 0 1 259 1 0.0 1 1 274 1 0.0 1 1