This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.83 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 84,467 (3.3%) Reads that were too short: 43,318 (1.7%) Reads written (passing filters): 41,149 (1.6%) Total basepairs processed: 774,927,510 bp Total written (filtered): 10,529,115 bp (1.4%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 84467 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 718 628.5 0 718 7 13765 628.5 0 13765 8 671 628.5 0 671 9 12046 628.5 0 12046 10 80 628.5 0 80 11 32 628.5 0 32 12 30 628.5 0 30 13 43 628.5 0 43 14 52 628.5 0 52 15 40 628.5 0 40 16 48 628.5 0 48 17 44 628.5 0 44 18 37 628.5 0 37 19 33 628.5 0 33 20 54 628.5 0 54 21 46 628.5 0 46 22 37 628.5 0 37 23 35 628.5 0 35 24 45 628.5 0 45 25 77 628.5 0 77 26 40 628.5 0 40 27 47 628.5 0 47 28 43 628.5 0 43 29 45 628.5 0 45 30 38 628.5 0 38 31 60 628.5 0 60 32 40 628.5 0 40 33 46 628.5 0 46 34 55 628.5 0 55 35 64 628.5 0 64 36 49 628.5 0 49 37 37 628.5 0 37 38 112 628.5 0 112 39 40 628.5 0 40 40 45 628.5 0 45 41 43 628.5 0 43 42 40 628.5 0 40 43 47 628.5 0 47 44 56 628.5 0 56 45 59 628.5 0 59 46 43 628.5 0 43 47 59 628.5 0 59 48 51 628.5 0 51 49 48 628.5 0 48 50 52 628.5 0 52 51 56 628.5 0 56 52 47 628.5 0 47 53 58 628.5 0 58 54 61 628.5 0 61 55 54 628.5 0 54 56 48 628.5 0 48 57 53 628.5 0 53 58 34 628.5 0 34 59 60 628.5 0 60 60 49 628.5 0 49 61 54 628.5 0 54 62 53 628.5 0 53 63 55 628.5 0 55 64 46 628.5 0 46 65 51 628.5 0 51 66 65 628.5 0 65 67 382 628.5 0 382 68 481 628.5 0 481 69 244 628.5 0 244 70 29 628.5 0 29 71 60 628.5 0 60 72 66 628.5 0 66 73 44 628.5 0 44 74 53 628.5 0 53 75 61 628.5 0 61 76 60 628.5 0 60 77 38 628.5 0 38 78 64 628.5 0 64 79 52 628.5 0 52 80 37 628.5 0 37 81 71 628.5 0 71 82 32 628.5 0 32 83 32 628.5 0 32 84 33 628.5 0 33 85 47 628.5 0 47 86 88 628.5 0 88 87 41 628.5 0 41 88 28 628.5 0 28 89 63 628.5 0 63 90 53 628.5 0 53 91 42 628.5 0 42 92 45 628.5 0 45 93 49 628.5 0 49 94 56 628.5 0 56 95 42 628.5 0 42 96 63 628.5 0 63 97 66 628.5 0 66 98 71 628.5 0 71 99 58 628.5 0 58 100 46 628.5 0 46 101 51 628.5 0 51 102 45 628.5 0 45 103 41 628.5 0 41 104 49 628.5 0 49 105 53 628.5 0 53 106 50 628.5 0 50 107 39 628.5 0 39 108 36 628.5 0 36 109 51 628.5 0 51 110 48 628.5 0 48 111 46 628.5 0 46 112 32 628.5 0 32 113 54 628.5 0 54 114 47 628.5 0 47 115 41 628.5 0 41 116 41 628.5 0 41 117 61 628.5 0 61 118 64 628.5 0 64 119 54 628.5 0 54 120 107 628.5 0 107 121 51 628.5 0 51 122 79 628.5 0 79 123 68 628.5 0 68 124 44 628.5 0 44 125 43 628.5 0 43 126 48 628.5 0 48 127 66 628.5 0 66 128 71 628.5 0 71 129 121 628.5 0 121 130 80 628.5 0 80 131 224 628.5 0 224 132 78 628.5 0 78 133 120 628.5 0 120 134 75 628.5 0 75 135 48 628.5 0 48 136 42 628.5 0 42 137 53 628.5 0 53 138 44 628.5 0 44 139 53 628.5 0 53 140 35 628.5 0 35 141 41 628.5 0 41 142 38 628.5 0 38 143 43 628.5 0 43 144 71 628.5 0 71 145 68 628.5 0 68 146 52 628.5 0 52 147 66 628.5 0 66 148 63 628.5 0 63 149 66 628.5 0 66 150 56 628.5 0 56 151 58 628.5 0 58 152 213 628.5 0 213 153 177 628.5 0 177 154 288 628.5 0 288 155 201 628.5 0 201 156 66 628.5 0 66 157 46 628.5 0 46 158 99 628.5 0 99 159 442 628.5 0 442 160 155 628.5 0 155 161 82 628.5 0 82 162 61 628.5 0 61 163 109 628.5 0 109 164 100 628.5 0 100 165 114 628.5 0 114 166 73 628.5 0 73 167 56 628.5 0 56 168 48 628.5 0 48 169 47 628.5 0 47 170 37 628.5 0 37 171 66 628.5 0 66 172 68 628.5 0 68 173 44 628.5 0 44 174 62 628.5 0 62 175 58 628.5 0 58 176 47 628.5 0 47 177 75 628.5 0 75 178 49 628.5 0 49 179 68 628.5 0 68 180 102 628.5 0 102 181 84 628.5 0 84 182 76 628.5 0 76 183 119 628.5 0 119 184 386 628.5 0 386 185 133 628.5 0 133 186 237 628.5 0 237 187 43 628.5 0 43 188 46 628.5 0 46 189 55 628.5 0 55 190 57 628.5 0 57 191 63 628.5 0 63 192 128 628.5 0 128 193 164 628.5 0 164 194 124 628.5 0 124 195 111 628.5 0 111 196 44 628.5 0 44 197 53 628.5 0 53 198 75 628.5 0 75 199 107 628.5 0 107 200 71 628.5 0 71 201 112 628.5 0 112 202 123 628.5 0 123 203 51 628.5 0 51 204 75 628.5 0 75 205 104 628.5 0 104 206 109 628.5 0 109 207 99 628.5 0 99 208 106 628.5 0 106 209 163 628.5 0 163 210 62 628.5 0 62 211 59 628.5 0 59 212 140 628.5 0 140 213 98 628.5 0 98 214 197 628.5 0 197 215 204 628.5 0 204 216 252 628.5 0 252 217 527 628.5 0 527 218 717 628.5 0 717 219 9550 628.5 0 9550 220 6945 628.5 0 6945 221 16161 628.5 0 16161 222 324 628.5 0 324 223 70 628.5 0 70 224 66 628.5 0 66 225 64 628.5 0 64 226 55 628.5 0 55 227 71 628.5 0 71 228 56 628.5 0 56 229 58 628.5 0 58 230 55 628.5 0 55 231 61 628.5 0 61 232 54 628.5 0 54 233 48 628.5 0 48 234 56 628.5 0 56 235 67 628.5 0 67 236 62 628.5 0 62 237 89 628.5 0 89 238 69 628.5 0 69 239 65 628.5 0 65 240 67 628.5 0 67 241 74 628.5 0 74 242 50 628.5 0 50 243 55 628.5 0 55 244 63 628.5 0 63 245 41 628.5 0 41 246 72 628.5 0 72 247 84 628.5 0 84 248 172 628.5 0 172 249 285 628.5 0 285 250 241 628.5 0 241 251 127 628.5 0 127 252 107 628.5 0 107 253 97 628.5 0 97 254 123 628.5 0 123 255 85 628.5 0 85 256 72 628.5 0 72 257 72 628.5 0 72 258 87 628.5 0 87 259 52 628.5 0 52 260 58 628.5 0 58 261 66 628.5 0 66 262 73 628.5 0 73 263 69 628.5 0 69 264 56 628.5 0 56 265 58 628.5 0 58 266 60 628.5 0 60 267 86 628.5 0 86 268 67 628.5 0 67 269 70 628.5 0 70 270 80 628.5 0 80 271 61 628.5 0 61 272 80 628.5 0 80 273 109 628.5 0 109 274 86 628.5 0 86 275 76 628.5 0 76 276 54 628.5 0 54 277 80 628.5 0 80 278 96 628.5 0 96 279 360 628.5 0 360 280 366 628.5 0 366 281 345 628.5 0 345 282 112 628.5 0 112 283 63 628.5 0 63 284 82 628.5 0 82 285 57 628.5 0 57 286 80 628.5 0 80 287 84 628.5 0 84 288 72 628.5 0 72 289 122 628.5 0 122 290 71 628.5 0 71 291 84 628.5 0 84 292 120 628.5 0 120 293 89 628.5 0 89 294 80 628.5 0 80 295 67 628.5 0 67 296 65 628.5 0 65 297 95 628.5 0 95 298 63 628.5 0 63 299 76 628.5 0 76 300 103 628.5 0 103 301 119 628.5 0 119 Finished in 1.93 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 41,149 Reads with adapters: 12,740 (31.0%) Reads that were too short: 56 (0.1%) Reads written (passing filters): 12,684 (30.8%) Total basepairs processed: 10,529,115 bp Total written (filtered): 3,481,795 bp (33.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12740 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 3 1 16 87 0.0 3 0 85 2 17 12484 0.0 3 12249 225 9 1 18 33 0.0 3 2 30 1 19 8 0.0 3 0 0 6 2 20 16 0.0 3 0 0 0 16 22 1 0.0 3 1 23 2 0.0 3 0 1 0 1 28 1 0.0 3 1 34 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 109 52 0.0 3 0 0 0 52 116 8 0.0 3 0 0 0 8 120 1 0.0 3 0 0 0 1 122 4 0.0 3 0 0 0 4 126 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 12,684 Reads with adapters: 82 (0.6%) Reads written (passing filters): 12,684 (100.0%) Total basepairs processed: 3,481,795 bp Total written (filtered): 3,481,524 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 82 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.9% C: 0.0% G: 81.7% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 59 198.2 0 59 4 22 49.5 0 22 6 1 3.1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.